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Q15742 (NAB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NGFI-A-binding protein 2
Alternative name(s):
EGR-1-binding protein 2
Melanoma-associated delayed early response protein
Short name=Protein MADER
Gene names
Name:NAB2
Synonyms:MADER
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability By similarity.

Subunit structure

Homomultimers may associate with EGR1 bound to DNA By similarity.

Subcellular location

Nucleus By similarity. Note: Isoform 2 is not localized to the nucleus By similarity.

Tissue specificity

Widely expressed at low levels. Highly expressed in melanoma cell lines.

Induction

By serum and phorbol myristate acetate (PMA) stimulation.

Domain

The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.

The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

Post-translational modification

Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain. Ref.12

Sequence similarities

Belongs to the NAB family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15742-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15742-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASP
     323-525: Missing.
Isoform 3 (identifier: Q15742-3)

The sequence of this isoform differs from the canonical sequence as follows:
     426-489: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525NGFI-A-binding protein 2
PRO_0000077042

Regions

Region35 – 11379NCD1
Region267 – 35690NCD2
Region353 – 38432Necessary for nuclear localization By similarity

Amino acid modifications

Modified residue61Phosphoserine Ref.9 Ref.11
Modified residue1591Phosphoserine Ref.13
Modified residue1621Phosphoserine Ref.13
Modified residue1711Phosphoserine By similarity
Cross-link379Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable
Cross-link517Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable

Natural variations

Alternative sequence320 – 3223LTI → ASP in isoform 2.
VSP_003385
Alternative sequence323 – 525203Missing in isoform 2.
VSP_003386
Alternative sequence426 – 48964Missing in isoform 3.
VSP_003387

Experimental info

Sequence conflict257 – 2582PR → Q Ref.6
Sequence conflict257 – 2582PR → Q Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 38CF6CFEFE3756F9

FASTA52556,594
        10         20         30         40         50         60 
MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET 

        70         80         90        100        110        120 
FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 

       130        140        150        160        170        180 
PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 

       190        200        210        220        230        240 
DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE 

       250        260        270        280        290        300 
MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI 

       310        320        330        340        350        360 
IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 

       370        380        390        400        410        420 
KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY VPPYRPSLEE DSASLSGESL 

       430        440        450        460        470        480 
DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP 

       490        500        510        520 
CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ 

« Hide

Isoform 2 [UniParc].

Checksum: 6C7ACFBFEAD113E0
Show »

FASTA32234,356
Isoform 3 [UniParc].

Checksum: F7C955EEFDAFBA94
Show »

FASTA46149,748

References

« Hide 'large scale' references
[1]"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli."
Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J.
Mol. Cell. Biol. 16:3545-3553(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Placenta.
[2]"Genomic organization of the Mader/NAB2 gene."
Gerlinger M., Johnson J.P.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Skin.
[6]"Mader: a novel nuclear protein over expressed in human melanomas."
Kirsch K.H., Korradi Y., Johnson J.P.
Oncogene 12:963-971(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 50-525 (ISOFORM 1).
[7]Johnson J.P.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 3).
[8]"The Nab2 and Stat6 genes share a common transcription termination region."
Svaren J., Apel E.D., Simburger K.S., Jenkins N.A., Gilbert D.J., Copeland N.G., Milbrandt J.
Genomics 41:33-39(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators."
Garcia-Gutierrez P., Juarez-Vicente F., Gallardo-Chamizo F., Charnay P., Garcia-Dominguez M.
EMBO Rep. 12:1018-1023(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION AT LYS-379 AND LYS-517 BY EGR2.
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159 AND SER-162, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48361 mRNA. Translation: AAC50589.1.
AF268380 Genomic DNA. Translation: AAF72545.1.
AK314229 mRNA. Translation: BAG36900.1.
CH471054 Genomic DNA. Translation: EAW96989.1.
BC065931 mRNA. Translation: AAH65931.1.
X70991 mRNA. Translation: CAA50318.1.
AJ011081 Genomic DNA. Translation: CAA09472.1.
CCDSCCDS8930.1. [Q15742-1]
RefSeqNP_005958.1. NM_005967.3. [Q15742-1]
XP_005268951.1. XM_005268894.2. [Q15742-3]
UniGeneHs.159223.

3D structure databases

ProteinModelPortalQ15742.
SMRQ15742. Positions 228-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110747. 7 interactions.
IntActQ15742. 2 interactions.
STRING9606.ENSP00000300131.

PTM databases

PhosphoSiteQ15742.

Polymorphism databases

DMDM12643729.

Proteomic databases

MaxQBQ15742.
PaxDbQ15742.
PRIDEQ15742.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300131; ENSP00000300131; ENSG00000166886. [Q15742-1]
ENST00000342556; ENSP00000341491; ENSG00000166886. [Q15742-3]
ENST00000357680; ENSP00000350309; ENSG00000166886. [Q15742-2]
GeneID4665.
KEGGhsa:4665.
UCSCuc001smz.3. human. [Q15742-1]

Organism-specific databases

CTD4665.
GeneCardsGC12P057457.
HGNCHGNC:7627. NAB2.
HPACAB004510.
HPA027464.
MIM602381. gene.
neXtProtNX_Q15742.
Orphanet2126. Solitary fibrous tumor.
PharmGKBPA31432.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG307377.
HOGENOMHOG000026777.
HOVERGENHBG003127.
InParanoidQ15742.
OMAPGPESYA.
OrthoDBEOG776SPQ.
PhylomeDBQ15742.
TreeFamTF315501.

Gene expression databases

BgeeQ15742.
CleanExHS_NAB2.
GenevestigatorQ15742.

Family and domain databases

InterProIPR006989. NAB_co-repressor_dom.
IPR006988. Nab_N.
[Graphical view]
PfamPF04904. NCD1. 1 hit.
PF04905. NCD2. 1 hit.
[Graphical view]
ProDomPD342192. Nab_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

GeneWikiNAB2.
GenomeRNAi4665.
NextBio17978.
PROQ15742.
SOURCESearch...

Entry information

Entry nameNAB2_HUMAN
AccessionPrimary (citable) accession number: Q15742
Secondary accession number(s): B2RAK3, O76006, Q14797
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM