Q15742 (NAB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NGFI-A-binding protein 2

Alternative name(s):
EGR-1-binding protein 2
Melanoma-associated delayed early response protein
Short name=Protein MADER

Gene names

Name:	NAB2
Synonyms:	MADER

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability By similarity.
Subunit structure	Homomultimers may associate with EGR1 bound to DNA By similarity.
Subcellular location	Nucleus By similarity. Note: Isoform 2 is not localized to the nucleus By similarity.
Tissue specificity	Widely expressed at low levels. Highly expressed in melanoma cell lines.
Induction	By serum and phorbol myristate acetate (PMA) stimulation.
Domain	The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization. The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Post-translational modification	Sumoylation by EGR2 represses EGR2 transcriptional activity in hindbrain.
Sequence similarities	Belongs to the NAB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Traceable author statement. Source: ProtInc negative regulation of transcription from RNA polymerase III promoter Inferred from direct assay Ref.1. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15742-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15742-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-322: LTI → ASP
     323-525: Missing.

Isoform 3 (identifier: Q15742-3)

The sequence of this isoform differs from the canonical sequence as follows:
     426-489: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	NGFI-A-binding protein 2		PRO_0000077042
Regions
	Region	35 – 113	79	NCD1
	Region	267 – 356	90	NCD2
	Region	353 – 384	32	Necessary for nuclear localization By similarity
Amino acid modifications
	Modified residue	6	1	Phosphoserine Ref.11 Ref.12 Ref.13
	Modified residue	159	1	Phosphoserine Ref.9
	Modified residue	162	1	Phosphoserine Ref.9 Ref.11
	Modified residue	171	1	Phosphoserine Ref.10
	Modified residue	479	1	Phosphoserine Ref.11
	Cross-link	379		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable
	Cross-link	517		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) Probable
Natural variations
	Alternative sequence	320 – 322	3	LTI → ASP in isoform 2.		VSP_003385
	Alternative sequence	323 – 525	203	Missing in isoform 2.		VSP_003386
	Alternative sequence	426 – 489	64	Missing in isoform 3.		VSP_003387
Experimental info
	Sequence conflict	257 – 258	2	PR → Q Ref.6
	Sequence conflict	257 – 258	2	PR → Q Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 38CF6CFEFE3756F9	FASTA	525	56,594
	10 20 30 40 50 60 MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET 70 80 90 100 110 120 FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 130 140 150 160 170 180 PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 190 200 210 220 230 240 DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE 250 260 270 280 290 300 MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI 310 320 330 340 350 360 IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 370 380 390 400 410 420 KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY VPPYRPSLEE DSASLSGESL 430 440 450 460 470 480 DGHLQAVGSC PRLTPPPADL PLALPAHGLW SRHILQQTLM DEGLRLARLV SHDRVGRLSP 490 500 510 520 CVPAKPPLAE FEEGLLDRCP APGPHPALVE GRRSSVKVEA EASRQ
	Isoform 2 [UniParc]. Checksum: 6C7ACFBFEAD113E0	FASTA	322	34,356
	10 20 30 40 50 60 MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET 70 80 90 100 110 120 FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 130 140 150 160 170 180 PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 190 200 210 220 230 240 DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE 250 260 270 280 290 300 MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI 310 320 IYGRFDSKRR EGKQLSLHEA SP
	Isoform 3 [UniParc]. Checksum: F7C955EEFDAFBA94	FASTA	461	49,748
	10 20 30 40 50 60 MHRAPSPTAE QPPGGGDSAR RTLQPRLKPS ARAMALPRTL GELQLYRVLQ RANLLSYYET 70 80 90 100 110 120 FIQQGGDDVQ QLCEAGEEEF LEIMALVGMA TKPLHVRRLQ KALREWATNP GLFSQPVPAV 130 140 150 160 170 180 PVSSIPLFKI SETAGTRKGS MSNGHGSPGE KAGSARSFSP KSPLELGEKL SPLPGGPGAG 190 200 210 220 230 240 DPRIWPGRST PESDVGAGGE EEAGSPPFSP PAGGGVPEGT GAGGLAAGGT GGGPDRLEPE 250 260 270 280 290 300 MVRMVVESVE RIFRSFPRGD AGEVTSLLKL NKKLARSVGH IFEMDDNDSQ KEEEIRKYSI 310 320 330 340 350 360 IYGRFDSKRR EGKQLSLHEL TINEAAAQFC MRDNTLLLRR VELFSLSRQV ARESTYLSSL 370 380 390 400 410 420 KGSRLHPEEL GGPPLKKLKQ EVGEQSHPEI QQPPPGPESY VPPYRPSLEE DSASLSGESL 430 440 450 460 DGHLQEFEEG LLDRCPAPGP HPALVEGRRS SVKVEAEASR Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NAB2, a corepressor of NGFI-A (Egr-1) and Krox20, is induced by proliferative and differentiative stimuli." Svaren J., Sevetson B.R., Apel E.D., Zimonjic D.B., Popescu N.C., Milbrandt J. Mol. Cell. Biol. 16:3545-3553(1996) [PubMed: 8668170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[2]	"Genomic organization of the Mader/NAB2 gene." Gerlinger M., Johnson J.P. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[6]	"Mader: a novel nuclear protein over expressed in human melanomas." Kirsch K.H., Korradi Y., Johnson J.P. Oncogene 12:963-971(1996) [PubMed: 8649813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 50-525 (ISOFORM 1).
[7]	Johnson J.P. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM 3).
[8]	"The Nab2 and Stat6 genes share a common transcription termination region." Svaren J., Apel E.D., Simburger K.S., Jenkins N.A., Gilbert D.J., Copeland N.G., Milbrandt J. Genomics 41:33-39(1997) [PubMed: 9126479] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-159 AND SER-162, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6; SER-162 AND SER-479, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"The transcription factor Krox20 is an E3 ligase that sumoylates its Nab coregulators." Garcia-Gutierrez P., Juarez-Vicente F., Gallardo-Chamizo F., Charnay P., Garcia-Dominguez M. EMBO Rep. 12:1018-1023(2011) [PubMed: 21836637] [Abstract] Cited for: SUMOYLATION AT LYS-379 AND LYS-517 BY EGR2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U48361 mRNA. Translation: AAC50589.1. AF268380 Genomic DNA. Translation: AAF72545.1. AK314229 mRNA. Translation: BAG36900.1. CH471054 Genomic DNA. Translation: EAW96989.1. BC065931 mRNA. Translation: AAH65931.1. X70991 mRNA. Translation: CAA50318.1. AJ011081 Genomic DNA. Translation: CAA09472.1.
IPI	IPI00019432. IPI00220866. IPI00220867.
RefSeq	NP_005958.1. NM_005967.3.
UniGene	Hs.159223.
3D structure databases
ProteinModelPortal	Q15742.
SMR	Q15742. Positions 234-365.
ModBase	Search...
Protein-protein interaction databases
STRING	Q15742.
PTM databases
PhosphoSite	Q15742.
Polymorphism databases
DMDM	12643729.
Proteomic databases
PRIDE	Q15742.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000300131; ENSP00000300131; ENSG00000166886.
GeneID	4665.
KEGG	hsa:4665.
UCSC	uc001smz.1. human.
Organism-specific databases
CTD	4665.
GeneCards	GC12P057457.
H-InvDB	HIX0010744.
HGNC	HGNC:7627. NAB2.
HPA	CAB004510. HPA027464.
MIM	602381. gene.
neXtProt	NX_Q15742.
PharmGKB	PA31432.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07949.
GeneTree	ENSGT00390000006330.
HOGENOM	HBG447154.
HOVERGEN	HBG003127.
InParanoid	Q15742.
OMA	PGPESYA.
OrthoDB	EOG4DZ1V7.
PhylomeDB	Q15742.
Gene expression databases
ArrayExpress	Q15742.
Bgee	Q15742.
CleanEx	HS_NAB2.
Genevestigator	Q15742.
GermOnline	ENSG00000166886. Homo sapiens.
Family and domain databases
InterPro	IPR006989. NAB_co-repressor_dom. IPR006988. Nab_N. [Graphical view]
Pfam	PF04904. NCD1. 1 hit. PF04905. NCD2. 1 hit. [Graphical view]
ProDom	PD342192. Nab_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	17978.
SOURCE	Search...

Entry information

Entry name

NAB2_HUMAN

Accession

Primary (citable) accession number: Q15742
Secondary accession number(s): B2RAK3, O76006, Q14797

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.