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Protein

Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A

Gene

INPP5J

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).By similarity

Catalytic activityi

D-myo-inositol 1,4,5-trisphosphate + H2O = myo-inositol 1,4-bisphosphate + phosphate.
1D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O = 1D-myo-inositol 1,3,4-trisphosphate + phosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS11950-MONOMER.
ZFISH:HS11950-MONOMER.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-HSA-1855204. Synthesis of IP3 and IP4 in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A (EC:3.1.3.56)
Alternative name(s):
Inositol polyphosphate 5-phosphatase J
Gene namesi
Name:INPP5J
Synonyms:PIB5PA, PIPP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:8956. INPP5J.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Predominantly localized to membrane ruffles.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • dendritic shaft Source: Ensembl
  • growth cone Source: Ensembl
  • plasma membrane Source: Ensembl
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi27124.
OpenTargetsiENSG00000185133.
PharmGKBiPA164720918.

Polymorphism and mutation databases

DMDMi116242713.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002097381 – 1006Phosphatidylinositol 4,5-bisphosphate 5-phosphatase AAdd BLAST1006

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei56Omega-N-methylarginine; alternateBy similarity1
Modified residuei65Omega-N-methylarginineBy similarity1
Modified residuei76Asymmetric dimethylarginineBy similarity1
Modified residuei83Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei83Omega-N-methylarginine; alternateBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei324PhosphoserineBy similarity1
Modified residuei903PhosphoserineCombined sources1
Modified residuei990PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ15735.
MaxQBiQ15735.
PaxDbiQ15735.
PeptideAtlasiQ15735.
PRIDEiQ15735.

PTM databases

DEPODiQ15735.
iPTMnetiQ15735.
PhosphoSitePlusiQ15735.

Expressioni

Gene expression databases

BgeeiENSG00000185133.
CleanExiHS_INPP5J.
ExpressionAtlasiQ15735. baseline and differential.
GenevisibleiQ15735. HS.

Organism-specific databases

HPAiHPA034539.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC23Q9UJX23EBI-10236940,EBI-396137
DNAJA3Q96EY1-23EBI-10236940,EBI-3952284
EPS8Q129293EBI-10236940,EBI-375576
GOLGA2Q083793EBI-10236940,EBI-618309
TRIM27P143733EBI-10236940,EBI-719493
TRIM54Q9BYV23EBI-10236940,EBI-2130429

Protein-protein interaction databases

BioGridi118015. 6 interactors.
IntActiQ15735. 16 interactors.
STRINGi9606.ENSP00000384534.

Structurei

3D structure databases

ProteinModelPortaliQ15735.
SMRiQ15735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni425 – 728CatalyticSequence analysisAdd BLAST304
Regioni729 – 840Required for ruffle localizationBy similarityAdd BLAST112

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi102 – 107RSXSXX motif 16
Motifi345 – 350SH3-bindingSequence analysis6
Motifi350 – 355RSXSXX motif 26
Motifi874 – 879RSXSXX motif 36
Motifi885 – 890RSXSXX motif 46
Motifi911 – 916RSXSXX motif 56

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 371Pro-richAdd BLAST250
Compositional biasi840 – 937Ser-richAdd BLAST98

Domaini

The 5 Arg-Ser-Xaa-Ser-Xaa-Xaa (RSXSXX) motifs may constitute binding sites for the 14-3-3 protein.

Sequence similaritiesi

Keywords - Domaini

Repeat, SH3-binding

Phylogenomic databases

eggNOGiKOG0565. Eukaryota.
COG5411. LUCA.
GeneTreeiENSGT00760000119075.
HOGENOMiHOG000046051.
HOVERGENiHBG082135.
InParanoidiQ15735.
KOiK01106.
OMAiNIYQVTF.
OrthoDBiEOG091G055L.
PhylomeDBiQ15735.
TreeFamiTF317034.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15735-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGQSSRGSR RPGTRAGLGS LPMPQGVAQT GAPSKVDSSF QLPAKKNAAL
60 70 80 90 100
GPSEPRLALA PVGPRAAMSA SSEGPRLALA SPRPILAPLC TPEGQKTATA
110 120 130 140 150
HRSSSLAPTS VGQLVMSASA GPKPPPATTG SVLAPTSLGL VMPASAGPRS
160 170 180 190 200
PPVTLGPNLA PTSRDQKQEP PASVGPKPTL AASGLSLALA SEEQPPELPS
210 220 230 240 250
TPSPVPSPVL SPTQEQALAP ASTASGAASV GQTSARKRDA PAPRPLPASE
260 270 280 290 300
GHLQPPAQTS GPTGSPPCIQ TSPDPRLSPS FRARPEALHS SPEDPVLPRP
310 320 330 340 350
PQTLPLDVGQ GPSEPGTHSP GLLSPTFRPG APSGQTVPPP LPKPPRSPSR
360 370 380 390 400
SPSHSPNRSP CVPPAPDMAL PRLGTQSTGP GRCLSPNLQA QEAPAPVTTS
410 420 430 440 450
SSTSTLSSSP WSAQPTWKSD PGFRITVVTW NVGTAMPPDD VTSLLHLGGG
460 470 480 490 500
DDSDGADMIA IGLQEVNSML NKRLKDALFT DQWSELFMDA LGPFNFVLVS
510 520 530 540 550
SVRMQGVILL LFAKYYHLPF LRDVQTDCTR TGLGGYWGNK GGVSVRLAAF
560 570 580 590 600
GHMLCFLNCH LPAHMDKAEQ RKDNFQTILS LQQFQGPGAQ GILDHDLVFW
610 620 630 640 650
FGDLNFRIES YDLHFVKFAI DSDQLHQLWE KDQLNMAKNT WPILKGFQEG
660 670 680 690 700
PLNFAPTFKF DVGTNKYDTS AKKRKPAWTD RILWKVKAPG GGPSPSGRKS
710 720 730 740 750
HRLQVTQHSY RSHMEYTVSD HKPVAAQFLL QFAFRDDMPL VRLEVADEWV
760 770 780 790 800
RPEQAVVRYR METVFARSSW DWIGLYRVGF RHCKDYVAYV WAKHEDVDGN
810 820 830 840 850
TYQVTFSEES LPKGHGDFIL GYYSHNHSIL IGITEPFQIS LPSSELASSS
860 870 880 890 900
TDSSGTSSEG EDDSTLELLA PKSRSPSPGK SKRHRSRSPG LARFPGLALR
910 920 930 940 950
PSSRERRGAS RSPSPQSRRL SRVAPDRSSN GSSRGSSEEG PSGLPGPWAF
960 970 980 990 1000
PPAVPRSLGL LPALRLETVD PGGGGSWGPD REALAPNSLS PSPQGHRGLE

EGGLGP
Length:1,006
Mass (Da):107,197
Last modified:October 17, 2006 - v3
Checksum:iADB6382AA33E15AC
GO
Isoform 2 (identifier: Q15735-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-367: Missing.

Show »
Length:639
Mass (Da):70,464
Checksum:i3ED5CD8BBB3398D0
GO
Isoform 3 (identifier: Q15735-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-423: Missing.

Note: No experimental confirmation available.
Show »
Length:638
Mass (Da):70,239
Checksum:i3F8ECF77ECC71C26
GO

Sequence cautioni

The sequence AAD15618 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti610 – 612SYD → ARG (Ref. 5) Curated3
Sequence conflicti662V → A in BAC86611 (PubMed:14702039).Curated1
Sequence conflicti791W → R in AAI09289 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_028107333S → I.Corresponds to variant rs12485025dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0072961 – 367Missing in isoform 2. 2 PublicationsAdd BLAST367
Alternative sequenceiVSP_02101756 – 423Missing in isoform 3. 1 PublicationAdd BLAST368

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092859 mRNA. Translation: BAG52616.1.
AK095944 mRNA. Translation: BAC04657.1.
AK126610 mRNA. Translation: BAC86611.1.
AC005005 Genomic DNA. Translation: AAD15618.1. Sequence problems.
CH471095 Genomic DNA. Translation: EAW59936.1.
BC109288 mRNA. Translation: AAI09289.1.
U45975 mRNA. Translation: AAB03216.1.
CCDSiCCDS46687.1. [Q15735-3]
CCDS63453.1. [Q15735-1]
CCDS63455.1. [Q15735-2]
RefSeqiNP_001002837.1. NM_001002837.2. [Q15735-3]
NP_001271214.1. NM_001284285.1. [Q15735-1]
NP_001271217.1. NM_001284288.1. [Q15735-2]
NP_001271218.1. NM_001284289.1. [Q15735-2]
XP_016884260.1. XM_017028771.1. [Q15735-1]
UniGeneiHs.517549.

Genome annotation databases

EnsembliENST00000331075; ENSP00000333262; ENSG00000185133. [Q15735-1]
ENST00000400294; ENSP00000383150; ENSG00000185133. [Q15735-2]
ENST00000404390; ENSP00000384534; ENSG00000185133. [Q15735-3]
ENST00000405300; ENSP00000384596; ENSG00000185133. [Q15735-2]
GeneIDi27124.
KEGGihsa:27124.
UCSCiuc003ajs.5. human. [Q15735-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK092859 mRNA. Translation: BAG52616.1.
AK095944 mRNA. Translation: BAC04657.1.
AK126610 mRNA. Translation: BAC86611.1.
AC005005 Genomic DNA. Translation: AAD15618.1. Sequence problems.
CH471095 Genomic DNA. Translation: EAW59936.1.
BC109288 mRNA. Translation: AAI09289.1.
U45975 mRNA. Translation: AAB03216.1.
CCDSiCCDS46687.1. [Q15735-3]
CCDS63453.1. [Q15735-1]
CCDS63455.1. [Q15735-2]
RefSeqiNP_001002837.1. NM_001002837.2. [Q15735-3]
NP_001271214.1. NM_001284285.1. [Q15735-1]
NP_001271217.1. NM_001284288.1. [Q15735-2]
NP_001271218.1. NM_001284289.1. [Q15735-2]
XP_016884260.1. XM_017028771.1. [Q15735-1]
UniGeneiHs.517549.

3D structure databases

ProteinModelPortaliQ15735.
SMRiQ15735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118015. 6 interactors.
IntActiQ15735. 16 interactors.
STRINGi9606.ENSP00000384534.

PTM databases

DEPODiQ15735.
iPTMnetiQ15735.
PhosphoSitePlusiQ15735.

Polymorphism and mutation databases

DMDMi116242713.

Proteomic databases

EPDiQ15735.
MaxQBiQ15735.
PaxDbiQ15735.
PeptideAtlasiQ15735.
PRIDEiQ15735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000331075; ENSP00000333262; ENSG00000185133. [Q15735-1]
ENST00000400294; ENSP00000383150; ENSG00000185133. [Q15735-2]
ENST00000404390; ENSP00000384534; ENSG00000185133. [Q15735-3]
ENST00000405300; ENSP00000384596; ENSG00000185133. [Q15735-2]
GeneIDi27124.
KEGGihsa:27124.
UCSCiuc003ajs.5. human. [Q15735-1]

Organism-specific databases

CTDi27124.
DisGeNETi27124.
GeneCardsiINPP5J.
HGNCiHGNC:8956. INPP5J.
HPAiHPA034539.
MIMi606481. gene.
neXtProtiNX_Q15735.
OpenTargetsiENSG00000185133.
PharmGKBiPA164720918.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0565. Eukaryota.
COG5411. LUCA.
GeneTreeiENSGT00760000119075.
HOGENOMiHOG000046051.
HOVERGENiHBG082135.
InParanoidiQ15735.
KOiK01106.
OMAiNIYQVTF.
OrthoDBiEOG091G055L.
PhylomeDBiQ15735.
TreeFamiTF317034.

Enzyme and pathway databases

BioCyciMetaCyc:HS11950-MONOMER.
ZFISH:HS11950-MONOMER.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
R-HSA-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-HSA-1855204. Synthesis of IP3 and IP4 in the cytosol.

Miscellaneous databases

GeneWikiiPIB5PA.
GenomeRNAii27124.
PROiQ15735.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185133.
CleanExiHS_INPP5J.
ExpressionAtlasiQ15735. baseline and differential.
GenevisibleiQ15735. HS.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPI5PA_HUMAN
AccessioniPrimary (citable) accession number: Q15735
Secondary accession number(s): B3KS54
, Q32M61, Q6ZTH6, Q8N902, Q9UDT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.