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Protein

ETS-related transcription factor Elf-2

Gene

ELF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.
Isoform 2 may function in repression of RUNX1-mediated transactivation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi208 – 29083ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

SignaLinkiQ15723.

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-2
Alternative name(s):
E74-like factor 2
New ETS-related factor
Gene namesi
Name:ELF2Imported
Synonyms:NERF1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:3317. ELF2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27745.

Polymorphism and mutation databases

DMDMi68052029.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593ETS-related transcription factor Elf-2PRO_0000204087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei185 – 1851Phosphoserine1 Publication
Modified residuei191 – 1911Phosphoserine1 Publication
Modified residuei372 – 3721Phosphoserine1 Publication
Modified residuei430 – 4301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ15723.
PaxDbiQ15723.
PRIDEiQ15723.

Expressioni

Tissue specificityi

Expressed in all fetal and adult tissues examined. Among fetal tissues, highest levels of expression detected in heart, lung, liver and kidney, and lower levels in brain. Among adult tissues, highest levels of expression detected in heart, placenta, lung, skeletal muscle, spleen, thymus, testis and ovary. Moderate expression in prostate, small intestine, kidney, liver and pancreas, and weak expression in colon, brain and peripheral blood lymphocytes.1 Publication

Gene expression databases

BgeeiQ15723.
CleanExiHS_ELF2.
ExpressionAtlasiQ15723. baseline and differential.
GenevisibleiQ15723. HS.

Organism-specific databases

HPAiHPA006057.

Interactioni

Subunit structurei

Interacts with the LIM domains of LMO2 (By similarity). Interacts via its N-terminal region with RUNX1.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RUNX1Q011962EBI-956941,EBI-925904

Protein-protein interaction databases

BioGridi108313. 11 interactions.
IntActiQ15723. 7 interactions.
MINTiMINT-4533234.
STRINGi9606.ENSP00000265495.

Structurei

3D structure databases

ProteinModelPortaliQ15723.
SMRiQ15723. Positions 181-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ETS family.Sequence Analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG291231.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000049253.
HOVERGENiHBG007183.
InParanoidiQ15723.
KOiK09428.
OMAiPMKKKKA.
OrthoDBiEOG73RBD1.
PhylomeDBiQ15723.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 51 Publication (identifier: Q15723-5) [UniParc]FASTAAdd to basket

Also known as: NERF-2b1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA
60 70 80 90 100
AQVLVYDDET YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE
110 120 130 140 150
ALLHMESPTC LRDSRSPVEV FVPPCVSTPE FIHAAMRPDV ITETVVEVST
160 170 180 190 200
EESEPMDTSP IPTSPDSHEP MKKKKVGRKP KTQQSPISNG SPELGIKKKP
210 220 230 240 250
REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL VDSKAVSKLW
260 270 280 290 300
GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID
310 320 330 340 350
DDKSETCNED LAGTTDEKSL ERVSLSAESL LKAASSVRSG KNSSPINCSR
360 370 380 390 400
AEKGVARVVN ITSPGHDASS RSPTTTASVS ATAAPRTVRV AMQVPVVMTS
410 420 430 440 450
LGQKISTVAV QSVNAGAPLI TSTSPTTATS PKVVIQTIPT VMPASTENGD
460 470 480 490 500
KITMQPAKII TIPATQLAQC QLQTKSNLTG SGSINIVGTP LAVRALTPVS
510 520 530 540 550
IAHGTPVMRL SMPTQQASGQ TPPRVISAVI KGPEVKSEAV AKKQEHDVKT
560 570 580 590
LQLVEEKPAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK
Length:593
Mass (Da):63,967
Last modified:June 21, 2005 - v2
Checksum:iB67013B453559910
GO
Isoform 11 Publication (identifier: Q15723-1) [UniParc]FASTAAdd to basket

Also known as: NERF-21 Publication

, NERF-2a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     117-129: PVEVFVPPCVSTP → P

Show »
Length:581
Mass (Da):62,711
Checksum:i5D5E3169FF486A90
GO
Isoform 21 Publication (identifier: Q15723-2) [UniParc]FASTAAdd to basket

Also known as: NERF-1a1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA
     117-129: PVEVFVPPCVSTP → P

Show »
Length:521
Mass (Da):56,133
Checksum:iA54F8DAA008C164D
GO
Isoform 31 Publication (identifier: Q15723-3) [UniParc]FASTAAdd to basket

Also known as: NERF-1b1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA

Show »
Length:533
Mass (Da):57,389
Checksum:iDA45A5D12ECFD7E6
GO
Isoform 4Curated (identifier: Q15723-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA
     175-203: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:504
Mass (Da):54,261
Checksum:i0C6A1D98E54342CE
GO

Sequence cautioni

The sequence AAH65025.1 differs from that shown. Reason: Frameshift at position 541. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7979MTSAV…ENVET → MATSLHEGPTNQLDLLIRA in isoform 2, isoform 3 and isoform 4. 3 PublicationsVSP_014154Add
BLAST
Alternative sequencei117 – 12913PVEVF…CVSTP → P in isoform 1 and isoform 2. 2 PublicationsVSP_014155Add
BLAST
Alternative sequencei175 – 20329Missing in isoform 4. 1 PublicationVSP_014156Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43188 mRNA. Translation: AAB37759.1.
U43189 mRNA. Translation: AAB37760.1.
U43189 mRNA. Translation: AAB37761.1.
AF256222 mRNA. Translation: AAF67195.1.
AF256223 mRNA. Translation: AAF67196.1.
AC024032 Genomic DNA. No translation available.
AC093602 Genomic DNA. No translation available.
BC034951 mRNA. Translation: AAH34951.1.
BC065025 mRNA. Translation: AAH65025.1. Frameshift.
CCDSiCCDS3744.1. [Q15723-1]
CCDS3745.1. [Q15723-3]
CCDS64062.1. [Q15723-2]
CCDS64063.1. [Q15723-4]
PIRiG02318.
RefSeqiNP_001263386.1. NM_001276457.1. [Q15723-4]
NP_001263387.1. NM_001276458.1. [Q15723-2]
NP_001263388.1. NM_001276459.1.
NP_006865.1. NM_006874.3. [Q15723-3]
NP_973728.1. NM_201999.2. [Q15723-1]
XP_005262860.1. XM_005262803.1. [Q15723-5]
XP_005262861.1. XM_005262804.1. [Q15723-1]
UniGeneiHs.634040.

Genome annotation databases

EnsembliENST00000358635; ENSP00000351458; ENSG00000109381. [Q15723-3]
ENST00000379549; ENSP00000368867; ENSG00000109381. [Q15723-4]
ENST00000379550; ENSP00000368868; ENSG00000109381. [Q15723-5]
ENST00000394235; ENSP00000377782; ENSG00000109381. [Q15723-1]
ENST00000510408; ENSP00000426997; ENSG00000109381. [Q15723-2]
GeneIDi1998.
KEGGihsa:1998.
UCSCiuc003ihm.2. human. [Q15723-3]
uc003ihp.1. human. [Q15723-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43188 mRNA. Translation: AAB37759.1.
U43189 mRNA. Translation: AAB37760.1.
U43189 mRNA. Translation: AAB37761.1.
AF256222 mRNA. Translation: AAF67195.1.
AF256223 mRNA. Translation: AAF67196.1.
AC024032 Genomic DNA. No translation available.
AC093602 Genomic DNA. No translation available.
BC034951 mRNA. Translation: AAH34951.1.
BC065025 mRNA. Translation: AAH65025.1. Frameshift.
CCDSiCCDS3744.1. [Q15723-1]
CCDS3745.1. [Q15723-3]
CCDS64062.1. [Q15723-2]
CCDS64063.1. [Q15723-4]
PIRiG02318.
RefSeqiNP_001263386.1. NM_001276457.1. [Q15723-4]
NP_001263387.1. NM_001276458.1. [Q15723-2]
NP_001263388.1. NM_001276459.1.
NP_006865.1. NM_006874.3. [Q15723-3]
NP_973728.1. NM_201999.2. [Q15723-1]
XP_005262860.1. XM_005262803.1. [Q15723-5]
XP_005262861.1. XM_005262804.1. [Q15723-1]
UniGeneiHs.634040.

3D structure databases

ProteinModelPortaliQ15723.
SMRiQ15723. Positions 181-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108313. 11 interactions.
IntActiQ15723. 7 interactions.
MINTiMINT-4533234.
STRINGi9606.ENSP00000265495.

Polymorphism and mutation databases

DMDMi68052029.

Proteomic databases

MaxQBiQ15723.
PaxDbiQ15723.
PRIDEiQ15723.

Protocols and materials databases

DNASUi1998.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358635; ENSP00000351458; ENSG00000109381. [Q15723-3]
ENST00000379549; ENSP00000368867; ENSG00000109381. [Q15723-4]
ENST00000379550; ENSP00000368868; ENSG00000109381. [Q15723-5]
ENST00000394235; ENSP00000377782; ENSG00000109381. [Q15723-1]
ENST00000510408; ENSP00000426997; ENSG00000109381. [Q15723-2]
GeneIDi1998.
KEGGihsa:1998.
UCSCiuc003ihm.2. human. [Q15723-3]
uc003ihp.1. human. [Q15723-1]

Organism-specific databases

CTDi1998.
GeneCardsiGC04M139978.
H-InvDBHIX0164010.
HGNCiHGNC:3317. ELF2.
HPAiHPA006057.
neXtProtiNX_Q15723.
PharmGKBiPA27745.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG291231.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000049253.
HOVERGENiHBG007183.
InParanoidiQ15723.
KOiK09428.
OMAiPMKKKKA.
OrthoDBiEOG73RBD1.
PhylomeDBiQ15723.
TreeFamiTF318679.

Enzyme and pathway databases

SignaLinkiQ15723.

Miscellaneous databases

ChiTaRSiELF2. human.
GeneWikiiELF2.
GenomeRNAii1998.
NextBioi35501567.
PROiQ15723.

Gene expression databases

BgeeiQ15723.
CleanExiHS_ELF2.
ExpressionAtlasiQ15723. baseline and differential.
GenevisibleiQ15723. HS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1."
    Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A.
    Mol. Cell. Biol. 16:5091-5106(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 5), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Fetal brain1 Publication, Fetal liver1 Publication and SpleenImported.
  2. Wilkinson D.A., Neale G.A.M., Mao S., Fernandes E.R., Davenport J.W., Naeve C.W., Goorha R.M.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Chronic myeloid leukemia cell.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
    Tissue: TestisImported.
  5. "Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias."
    Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.
    Mol. Cell. Biol. 19:3635-3644(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RUNX1.
  6. "Isoforms of the Ets transcription factor NERF/ELF-2 physically interact with AML1 and mediate opposing effects on AML1-mediated transcription of the B cell-specific blk gene."
    Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.
    J. Biol. Chem. 279:19512-19522(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH RUNX1.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiELF2_HUMAN
AccessioniPrimary (citable) accession number: Q15723
Secondary accession number(s): E9PCX3
, Q15724, Q15725, Q6P1K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.