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Q15723 (ELF2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ETS-related transcription factor Elf-2
Alternative name(s):
E74-like factor 2
New ETS-related factor
Gene names
Name:ELF2
Synonyms:NERF
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter. Ref.1 Ref.5

Isoform 2 may function in repression of RUNX1-mediated transactivation. Ref.1 Ref.5

Subunit structure

Interacts with the LIM domains of LMO2 By similarity. Interacts via its N-terminal region with RUNX1. Ref.4 Ref.5 UniProtKB Q9JHC9

Subcellular location

Nucleus.

Tissue specificity

Expressed in all fetal and adult tissues examined. Among fetal tissues, highest levels of expression detected in heart, lung, liver and kidney, and lower levels in brain. Among adult tissues, highest levels of expression detected in heart, placenta, lung, skeletal muscle, spleen, thymus, testis and ovary. Moderate expression in prostate, small intestine, kidney, liver and pancreas, and weak expression in colon, brain and peripheral blood lymphocytes. Ref.1

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RUNX1Q011962EBI-956941,EBI-925904

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 5 Ref.1 (identifier: Q15723-5)

Also known as: NERF-2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 Ref.1 (identifier: Q15723-1)

Also known as: NERF-2; NERF-2a;

The sequence of this isoform differs from the canonical sequence as follows:
     117-129: PVEVFVPPCVSTP → P
Isoform 2 Ref.1 (identifier: Q15723-2)

Also known as: NERF-1a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA
     117-129: PVEVFVPPCVSTP → P
Isoform 3 Ref.1 (identifier: Q15723-3)

Also known as: NERF-1b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA
Isoform 4 (identifier: Q15723-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA
     175-203: Missing.
     544-546: QEH → ART
     547-593: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 593593ETS-related transcription factor Elf-2
PRO_0000204087

Regions

DNA binding208 – 29083ETS

Amino acid modifications

Modified residue1851Phosphoserine Ref.9
Modified residue1911Phosphoserine Ref.8
Modified residue3721Phosphoserine Ref.6
Modified residue4301Phosphoserine Ref.9

Natural variations

Alternative sequence1 – 7979MTSAV…ENVET → MATSLHEGPTNQLDLLIRA in isoform 2, isoform 3 and isoform 4. Ref.1
VSP_014154
Alternative sequence117 – 12913PVEVF…CVSTP → P in isoform 1 and isoform 2. Ref.1
VSP_014155
Alternative sequence175 – 20329Missing in isoform 4.
VSP_014156
Alternative sequence544 – 5463QEH → ART in isoform 4.
VSP_014157
Alternative sequence547 – 59347Missing in isoform 4.
VSP_014158

Sequences

Sequence LengthMass (Da)Tools
Isoform 5 (NERF-2b) [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: B67013B453559910

FASTA59363,967
        10         20         30         40         50         60 
MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET 

        70         80         90        100        110        120 
YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 

       130        140        150        160        170        180 
FVPPCVSTPE FIHAAMRPDV ITETVVEVST EESEPMDTSP IPTSPDSHEP MKKKKVGRKP 

       190        200        210        220        230        240 
KTQQSPISNG SPELGIKKKP REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL 

       250        260        270        280        290        300 
VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID 

       310        320        330        340        350        360 
DDKSETCNED LAGTTDEKSL ERVSLSAESL LKAASSVRSG KNSSPINCSR AEKGVARVVN 

       370        380        390        400        410        420 
ITSPGHDASS RSPTTTASVS ATAAPRTVRV AMQVPVVMTS LGQKISTVAV QSVNAGAPLI 

       430        440        450        460        470        480 
TSTSPTTATS PKVVIQTIPT VMPASTENGD KITMQPAKII TIPATQLAQC QLQTKSNLTG 

       490        500        510        520        530        540 
SGSINIVGTP LAVRALTPVS IAHGTPVMRL SMPTQQASGQ TPPRVISAVI KGPEVKSEAV 

       550        560        570        580        590 
AKKQEHDVKT LQLVEEKPAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK 

« Hide

Isoform 1 (NERF-2) (NERF-2a) [UniParc].

Checksum: 5D5E3169FF486A90
Show »

FASTA58162,711
Isoform 2 (NERF-1a) [UniParc].

Checksum: A54F8DAA008C164D
Show »

FASTA52156,133
Isoform 3 (NERF-1b) [UniParc].

Checksum: DA45A5D12ECFD7E6
Show »

FASTA53357,389
Isoform 4 [UniParc].

Checksum: F295506C5509D17C
Show »

FASTA45749,265

References

« Hide 'large scale' references
[1]"Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1."
Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A.
Mol. Cell. Biol. 16:5091-5106(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 5), FUNCTION, TISSUE SPECIFICITY.
Tissue: Fetal brain, Fetal liver and Spleen.
[2]Wilkinson D.A., Neale G.A.M., Mao S., Fernandes E.R., Davenport J.W., Naeve C.W., Goorha R.M.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Chronic myeloid leukemia cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
Tissue: Testis.
[4]"Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias."
Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D.
Mol. Cell. Biol. 19:3635-3644(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RUNX1.
[5]"Isoforms of the Ets transcription factor NERF/ELF-2 physically interact with AML1 and mediate opposing effects on AML1-mediated transcription of the B cell-specific blk gene."
Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A.
J. Biol. Chem. 279:19512-19522(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH RUNX1.
[6]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[7]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[9]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-430, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43188 mRNA. Translation: AAB37759.1.
U43189 mRNA. Translation: AAB37760.1.
U43189 mRNA. Translation: AAB37761.1.
AF256222 mRNA. Translation: AAF67195.1.
AF256223 mRNA. Translation: AAF67196.1.
BC034951 mRNA. Translation: AAH34951.1.
BC065025 mRNA. Translation: AAH65025.1.
PIRG02318.
RefSeqNP_001263386.1. NM_001276457.1.
NP_001263387.1. NM_001276458.1.
NP_001263388.1. NM_001276459.1.
NP_006865.1. NM_006874.3.
NP_973728.1. NM_201999.2.
XP_005262860.1. XM_005262803.1.
XP_005262861.1. XM_005262804.1.
UniGeneHs.634040.

3D structure databases

ProteinModelPortalQ15723.
SMRQ15723. Positions 210-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108313. 7 interactions.
IntActQ15723. 7 interactions.
MINTMINT-4533234.
STRING9606.ENSP00000265495.

Polymorphism databases

DMDM68052029.

Proteomic databases

PaxDbQ15723.
PRIDEQ15723.

Protocols and materials databases

DNASU1998.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000265495; ENSP00000265495; ENSG00000109381. [Q15723-1]
ENST00000358635; ENSP00000351458; ENSG00000109381. [Q15723-3]
ENST00000379550; ENSP00000368868; ENSG00000109381. [Q15723-5]
ENST00000394235; ENSP00000377782; ENSG00000109381. [Q15723-1]
ENST00000510408; ENSP00000426997; ENSG00000109381. [Q15723-2]
GeneID1998.
KEGGhsa:1998.
UCSCuc003ihm.2. human. [Q15723-3]
uc003ihp.1. human. [Q15723-1]

Organism-specific databases

CTD1998.
GeneCardsGC04M139978.
H-InvDBHIX0164010.
HGNCHGNC:3317. ELF2.
HPAHPA006057.
neXtProtNX_Q15723.
PharmGKBPA27745.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG291231.
HOGENOMHOG000049253.
HOVERGENHBG007183.
InParanoidQ15723.
KOK09428.
OMALTQCQLQ.
OrthoDBEOG73RBD1.
PhylomeDBQ15723.
TreeFamTF318679.

Enzyme and pathway databases

SignaLinkQ15723.

Gene expression databases

ArrayExpressQ15723.
BgeeQ15723.
CleanExHS_ELF2.
GenevestigatorQ15723.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiELF2.
GenomeRNAi1998.
NextBio8081.
PROQ15723.

Entry information

Entry nameELF2_HUMAN
AccessionPrimary (citable) accession number: Q15723
Secondary accession number(s): Q15724, Q15725, Q6P1K5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM