ETS-related transcription factor Elf-2

Names and origin

Protein names

Recommended name:
    ETS-related transcription factor Elf-2
Alternative name(s):
    E74-like factor 2
    New ETS-related factor

Gene names

Name:	ELF2
Synonyms:	NERF

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter. Isoform 2 may function in repression of RUNX1-mediated transactivation. Ref.1 Ref.5
Subunit structure	Interacts with the LIM domains of LMO2 By similarity. Interacts via its N-terminal region with RUNX1. Ref.5 Ref.4 UniProtKB Q9JHC9
Subcellular location	Nucleus.
Tissue specificity	Expressed in all fetal and adult tissues examined. Among fetal tissues, highest levels of expression detected in heart, lung, liver and kidney, and lower levels in brain. Among adult tissues, highest levels of expression detected in heart, placenta, lung, skeletal muscle, spleen, thymus, testis and ovary. Moderate expression in prostate, small intestine, kidney, liver and pancreas, and weak expression in colon, brain and peripheral blood lymphocytes. Ref.1
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleus Ref.5 Inferred by curator. Source: UniProtKB
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB sequence-specific DNA binding Inferred from direct assay. Source: UniProtKB transcription activator activity Ref.1 Inferred from direct assay. Source: UniProtKB transcription factor activity Inferred from direct assay. Source: UniProtKB transcription repressor activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
RUNX1	Q01196	2	EBI-956941,EBI-925904

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 5 Ref.1 (identifier: Q15723-5) Also known as: NERF-2b; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 Ref.1 (identifier: Q15723-1) Also known as: NERF-2; NERF-2a; The sequence of this isoform differs from the canonical sequence as follows: 117-129: PVEVFVPPCVSTP → P

Isoform 2 Ref.1 (identifier: Q15723-2) Also known as: NERF-1a; The sequence of this isoform differs from the canonical sequence as follows: 1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA 117-129: PVEVFVPPCVSTP → P

Isoform 3 Ref.1 (identifier: Q15723-3) Also known as: NERF-1b; The sequence of this isoform differs from the canonical sequence as follows: 1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA

Isoform 4 (identifier: Q15723-4) The sequence of this isoform differs from the canonical sequence as follows: 1-79: MTSAVVDSGG...QEVETENVET → MATSLHEGPTNQLDLLIRA 175-203: Missing. 544-546: QEH → ART 547-593: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 593

593

ETS-related transcription factor Elf-2

PRO_0000204087

Regions

DNA binding

208 – 290

83

ETS

Amino acid modifications

Modified residue

191

1

Phosphoserine Ref.6

Modified residue

363

1

Phosphoserine Ref.7

Modified residue

372

1

Phosphoserine Ref.7

Natural variations

Alternative sequence

1 – 79

79

MTSAV…ENVET → MATSLHEGPTNQLDLLIRA in isoform 2, isoform 3 and isoform 4. Ref.1

VSP_014154

Alternative sequence

117 – 129

13

PVEVF…CVSTP → P in isoform 1 and isoform 2. Ref.1

VSP_014155

Alternative sequence

175 – 203

29

Missing in isoform 4.

VSP_014156

Alternative sequence

544 – 546

3

QEH → ART in isoform 4.

VSP_014157

Alternative sequence

547 – 593

47

Missing in isoform 4.

VSP_014158

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Sequences

Sequence		Length	Mass (Da)
Isoform 5 (NERF-2b) [UniParc]. Last modified June 21, 2005. Version 2. Checksum: B67013B453559910 Show »	FASTA	593	63,967
10 20 30 40 50 60 MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET 70 80 90 100 110 120 YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 130 140 150 160 170 180 FVPPCVSTPE FIHAAMRPDV ITETVVEVST EESEPMDTSP IPTSPDSHEP MKKKKVGRKP 190 200 210 220 230 240 KTQQSPISNG SPELGIKKKP REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL 250 260 270 280 290 300 VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID 310 320 330 340 350 360 DDKSETCNED LAGTTDEKSL ERVSLSAESL LKAASSVRSG KNSSPINCSR AEKGVARVVN 370 380 390 400 410 420 ITSPGHDASS RSPTTTASVS ATAAPRTVRV AMQVPVVMTS LGQKISTVAV QSVNAGAPLI 430 440 450 460 470 480 TSTSPTTATS PKVVIQTIPT VMPASTENGD KITMQPAKII TIPATQLAQC QLQTKSNLTG 490 500 510 520 530 540 SGSINIVGTP LAVRALTPVS IAHGTPVMRL SMPTQQASGQ TPPRVISAVI KGPEVKSEAV 550 560 570 580 590 AKKQEHDVKT LQLVEEKPAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK « Hide
Isoform 1 (NERF-2) (NERF-2a). Checksum: 5D5E3169FF486A90 Show »	FASTA	581	62,711
10 20 30 40 50 60 MTSAVVDSGG TILELSSNGV ENQEESEKVS EYPAVIVEPV PSARLEQGYA AQVLVYDDET 70 80 90 100 110 120 YMMQDVAEEQ EVETENVETV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI 130 140 150 160 170 180 HAAMRPDVIT ETVVEVSTEE SEPMDTSPIP TSPDSHEPMK KKKVGRKPKT QQSPISNGSP 190 200 210 220 230 240 ELGIKKKPRE GKGNTTYLWE FLLDLLQDKN TCPRYIKWTQ REKGIFKLVD SKAVSKLWGK 250 260 270 280 290 300 HKNKPDMNYE TMGRALRYYY QRGILAKVEG QRLVYQFKDM PKNIVVIDDD KSETCNEDLA 310 320 330 340 350 360 GTTDEKSLER VSLSAESLLK AASSVRSGKN SSPINCSRAE KGVARVVNIT SPGHDASSRS 370 380 390 400 410 420 PTTTASVSAT AAPRTVRVAM QVPVVMTSLG QKISTVAVQS VNAGAPLITS TSPTTATSPK 430 440 450 460 470 480 VVIQTIPTVM PASTENGDKI TMQPAKIITI PATQLAQCQL QTKSNLTGSG SINIVGTPLA 490 500 510 520 530 540 VRALTPVSIA HGTPVMRLSM PTQQASGQTP PRVISAVIKG PEVKSEAVAK KQEHDVKTLQ 550 560 570 580 LVEEKPADGN KTVTHVVVVS APSAIALPVT MKTEGLVTCE K « Hide
Isoform 2 (NERF-1a). Checksum: A54F8DAA008C164D Show »	FASTA	521	56,133
10 20 30 40 50 60 MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPEFI 70 80 90 100 110 120 HAAMRPDVIT ETVVEVSTEE SEPMDTSPIP TSPDSHEPMK KKKVGRKPKT QQSPISNGSP 130 140 150 160 170 180 ELGIKKKPRE GKGNTTYLWE FLLDLLQDKN TCPRYIKWTQ REKGIFKLVD SKAVSKLWGK 190 200 210 220 230 240 HKNKPDMNYE TMGRALRYYY QRGILAKVEG QRLVYQFKDM PKNIVVIDDD KSETCNEDLA 250 260 270 280 290 300 GTTDEKSLER VSLSAESLLK AASSVRSGKN SSPINCSRAE KGVARVVNIT SPGHDASSRS 310 320 330 340 350 360 PTTTASVSAT AAPRTVRVAM QVPVVMTSLG QKISTVAVQS VNAGAPLITS TSPTTATSPK 370 380 390 400 410 420 VVIQTIPTVM PASTENGDKI TMQPAKIITI PATQLAQCQL QTKSNLTGSG SINIVGTPLA 430 440 450 460 470 480 VRALTPVSIA HGTPVMRLSM PTQQASGQTP PRVISAVIKG PEVKSEAVAK KQEHDVKTLQ 490 500 510 520 LVEEKPADGN KTVTHVVVVS APSAIALPVT MKTEGLVTCE K « Hide
Isoform 3 (NERF-1b). Checksum: DA45A5D12ECFD7E6 Show »	FASTA	533	57,389
10 20 30 40 50 60 MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 70 80 90 100 110 120 FVPPCVSTPE FIHAAMRPDV ITETVVEVST EESEPMDTSP IPTSPDSHEP MKKKKVGRKP 130 140 150 160 170 180 KTQQSPISNG SPELGIKKKP REGKGNTTYL WEFLLDLLQD KNTCPRYIKW TQREKGIFKL 190 200 210 220 230 240 VDSKAVSKLW GKHKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK DMPKNIVVID 250 260 270 280 290 300 DDKSETCNED LAGTTDEKSL ERVSLSAESL LKAASSVRSG KNSSPINCSR AEKGVARVVN 310 320 330 340 350 360 ITSPGHDASS RSPTTTASVS ATAAPRTVRV AMQVPVVMTS LGQKISTVAV QSVNAGAPLI 370 380 390 400 410 420 TSTSPTTATS PKVVIQTIPT VMPASTENGD KITMQPAKII TIPATQLAQC QLQTKSNLTG 430 440 450 460 470 480 SGSINIVGTP LAVRALTPVS IAHGTPVMRL SMPTQQASGQ TPPRVISAVI KGPEVKSEAV 490 500 510 520 530 AKKQEHDVKT LQLVEEKPAD GNKTVTHVVV VSAPSAIALP VTMKTEGLVT CEK « Hide
Isoform 4. Checksum: F295506C5509D17C Show »	FASTA	457	49,265
10 20 30 40 50 60 MATSLHEGPT NQLDLLIRAV EASVHSSNAH CTDKTIEAAE ALLHMESPTC LRDSRSPVEV 70 80 90 100 110 120 FVPPCVSTPE FIHAAMRPDV ITETVVEVST EESEPMDTSP IPTSPDSHEP MKKKKGNTTY 130 140 150 160 170 180 LWEFLLDLLQ DKNTCPRYIK WTQREKGIFK LVDSKAVSKL WGKHKNKPDM NYETMGRALR 190 200 210 220 230 240 YYYQRGILAK VEGQRLVYQF KDMPKNIVVI DDDKSETCNE DLAGTTDEKS LERVSLSAES 250 260 270 280 290 300 LLKAASSVRS GKNSSPINCS RAEKGVARVV NITSPGHDAS SRSPTTTASV SATAAPRTVR 310 320 330 340 350 360 VAMQVPVVMT SLGQKISTVA VQSVNAGAPL ITSTSPTTAT SPKVVIQTIP TVMPASTENG 370 380 390 400 410 420 DKITMQPAKI ITIPATQLAQ CQLQTKSNLT GSGSINIVGT PLAVRALTPV SIAHGTPVMR 430 440 450 LSMPTQQASG QTPPRVISAV IKGPEVKSEA VAKKART « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of NERF, a novel transcription factor related to the Ets factor ELF-1." Oettgen P., Akbarali Y., Boltax J., Best J., Kunsch C., Libermann T.A. Mol. Cell. Biol. 16:5091-5106(1996) [PubMed: 8756667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 5), FUNCTION, TISSUE SPECIFICITY. Tissue: Fetal brain, Fetal liver and Spleen.
[2]	Wilkinson D.A., Neale G.A.M., Mao S., Fernandes E.R., Davenport J.W., Naeve C.W., Goorha R.M. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Chronic myeloid leukemia cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Testis.
[4]	"Functional and physical interactions between AML1 proteins and an ETS protein, MEF: implications for the pathogenesis of t(8;21)-positive leukemias." Mao S., Frank R.C., Zhang J., Miyazaki Y., Nimer S.D. Mol. Cell. Biol. 19:3635-3644(1999) [PubMed: 10207087] [Abstract] Cited for: INTERACTION WITH RUNX1.
[5]	"Isoforms of the Ets transcription factor NERF/ELF-2 physically interact with AML1 and mediate opposing effects on AML1-mediated transcription of the B cell-specific blk gene." Cho J.-Y., Akbarali Y., Zerbini L.F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D.-E., Libermann T.A. J. Biol. Chem. 279:19512-19522(2004) [PubMed: 14970218] [Abstract] Cited for: FUNCTION, INTERACTION WITH RUNX1.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-363 AND SER-372, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U43188 mRNA. Translation: AAB37759.1. U43189 mRNA. Translation: AAB37760.1. U43189 mRNA. Translation: AAB37761.1. AF256222 mRNA. Translation: AAF67195.1. AF256223 mRNA. Translation: AAF67196.1. BC034951 mRNA. Translation: AAH34951.1. BC065025 mRNA. Translation: AAH65025.1.
IPI	IPI00009995. IPI00396265. IPI00439925. IPI00607557. IPI00607896.
PIR	G02318.
RefSeq	NP_006865.1. NP_973728.1.
UniGene	Hs.634040
3D structure databases
HSSP	HSSP built from PDB template 2STT based on UniProtKB P14921.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15723. 1 interaction.
STRING	Q15723.
PTM databases
PhosphoSite	Q15723.
Genome annotation databases
Ensembl	ENST00000265495; ENSP00000265495; ENSG00000109381; Homo sapiens. [Genome view] ENST00000358635; ENSP00000351458; ENSG00000109381; Homo sapiens. [Genome view] ENST00000379549; ENSP00000368867; ENSG00000109381; Homo sapiens. [Genome view] ENST00000379550; ENSP00000368868; ENSG00000109381; Homo sapiens. [Genome view] ENST00000394235; ENSP00000377782; ENSG00000109381; Homo sapiens. [Genome view] ENST00000420916; ENSP00000397796; ENSG00000109381; Homo sapiens. [Genome view]
GeneID	1998.
KEGG	hsa:1998.
UCSC	uc003ihm.1. human. uc003ihn.1. human. uc003iho.1. human. uc003ihp.1. human.
Organism-specific databases
CTD	1998.
GeneCards	GC04M140198.
H-InvDB	HIX0024562.
HGNC	HGNC:3317. ELF2.
HPA	HPA006057.
PharmGKB	PA27745.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q15723.
HOVERGEN	Q15723.
OMA	VPTQQAS.
Enzyme and pathway databases
Pathway_Interaction_DB	angiopoietinreceptor_pathway. Angiopoietin receptor Tie2-mediated signaling.
Gene expression databases
ArrayExpress	Q15723.
Bgee	Q15723.
CleanEx	HS_ELF2.
Genevestigator	Q15723.
GermOnline	ENSG00000109381. Homo sapiens.
Family and domain databases
InterPro	IPR000418. Ets. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	8081.

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Entry information

Entry name

ELF2_HUMAN

Accession

Primary (citable) accession number: Q15723
Secondary accession number(s): Q15724, Q15725, Q6P1K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents