Q15722 (LT4R1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leukotriene B4 receptor 1
Short name=LTB4-R 1
Short name=LTB4-R1

Alternative name(s):
Chemoattractant receptor-like 1
G-protein coupled receptor 16
P2Y purinoceptor 7
Short name=P2Y7

Gene names

Name:	LTB4R
Synonyms:	BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
Post-translational modification	Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization. Ref.9
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Sequence caution	The sequence AAB16747.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Traceable author statement Ref.3. Source: ProtInc immune response Traceable author statement Ref.3. Source: ProtInc inflammatory response Traceable author statement Ref.3. Source: ProtInc muscle contraction Traceable author statement Ref.1. Source: ProtInc phospholipase C-activating G-protein coupled receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.3. Source: ProtInc
Molecular_function	leukotriene B4 receptor activity Inferred from electronic annotation. Source: Compara leukotriene receptor activity Traceable author statement Ref.3. Source: ProtInc nucleotide binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Leukotriene B4 receptor 1		PRO_0000069708
Regions
	Topological domain	1 – 19	19	Extracellular Potential
	Transmembrane	20 – 42	23	Helical; Name=1; Potential
	Topological domain	43 – 54	12	Cytoplasmic Potential
	Transmembrane	55 – 75	21	Helical; Name=2; Potential
	Topological domain	76 – 91	16	Extracellular Potential
	Transmembrane	92 – 113	22	Helical; Name=3; Potential
	Topological domain	114 – 138	25	Cytoplasmic Potential
	Transmembrane	139 – 159	21	Helical; Name=4; Potential
	Topological domain	160 – 178	19	Extracellular Potential
	Transmembrane	179 – 199	21	Helical; Name=5; Potential
	Topological domain	200 – 221	22	Cytoplasmic Potential
	Transmembrane	222 – 242	21	Helical; Name=6; Potential
	Topological domain	243 – 268	26	Extracellular Potential
	Transmembrane	269 – 289	21	Helical; Name=7; Potential
	Topological domain	290 – 352	63	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	346	1	L → F. Ref.7 Ref.8 Corresponds to variant rs17849864 [ dbSNP | Ensembl ].		VAR_060679
Experimental info
	Mutagenesis	308	1	T → P or A: No effect on affinity for leukotriene B4, induces resistance to desensitization by GRK6, but minor effect on phosphorylation by GRK6. Ref.9
	Mutagenesis	310	1	S → A: No effect on affinity for leukotriene B4 or on desensitization by GRK6. Ref.9
	Sequence conflict	246	1	G → R in CAA67001. Ref.4
	Sequence conflict	272	1	A → V in AAC50628. Ref.1
	Sequence conflict	293	1	L → V in AAC50628. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q15722 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 5A7BFC0A659AC81C	FASTA	352	37,557
	10 20 30 40 50 60 MNTTSSAAPP SLGVEFISLL AIILLSVALA VGLPGNSFVV WSILKRMQKR SVTALMVLNL 70 80 90 100 110 120 ALADLAVLLT APFFLHFLAQ GTWSFGLAGC RLCHYVCGVS MYASVLLITA MSLDRSLAVA 130 140 150 160 170 180 RPFVSQKLRT KAMARRVLAG IWVLSFLLAT PVLAYRTVVP WKTNMSLCFP RYPSEGHRAF 190 200 210 220 230 240 HLIFEAVTGF LLPFLAVVAS YSDIGRRLQA RRFRRSRRTG RLVVLIILTF AAFWLPYHVV 250 260 270 280 290 300 NLAEAGRALA GQAAGLGLVG KRLSLARNVL IALAFLSSSV NPVLYACAGG GLLRSAGVGF 310 320 330 340 350 VAKLLEGTGS EASSTRRGGS LGQTARSGPA ALEPGPSESL TASSPLKLNE LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells." Akbar G.K.M., Dasari V.R., Webb T., Ayyanathan K., Pillarisetti K., Sandhu A.K., Athwal R.S., Daniel J.L., Ashby B., Barnard E.A., Kunapuli S.P. J. Biol. Chem. 271:18363-18367(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"New members of the chemokine receptor gene family." Raport C.J., Schweickart V.L., Chantry D., Eddy R.L. Jr., Shows T.B., Godiska R., Gray P.W. J. Leukoc. Biol. 59:18-23(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis." Yokomizo T., Izumi T., Chang K., Takuwa Y., Shimizu T. Nature 387:620-624(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of cDNA encoding a putative chemoattractant receptor." Owman C.S.O., Nilsson C., Lolait S.J. Genomics 37:187-194(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cell-specific transcriptional regulation of human leukotriene B(4) receptor gene." Kato K., Yokomizo T., Izumi T., Shimizu T. J. Exp. Med. 192:413-420(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Isolation of complete coding sequence for leukotriene B4 receptor (LTB4R)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PHE-346.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PHE-346. Tissue: Lung.
[9]	"Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization." Gaudreau R., Le Gouill C., Venne M.-H., Stankova J., Rola-Pleszczynski M. J. Biol. Chem. 277:31567-31576(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-308 AND SER-310.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41070 mRNA. Translation: AAC50628.1. U33448 Genomic DNA. Translation: AAB16747.1. Different initiation. D89079 mRNA. Translation: BAA20424.1. D89078 mRNA. Translation: BAA20423.1. X98356 mRNA. Translation: CAA67001.1. AB008193 Genomic DNA. Translation: BAB00611.1. AY322535 Genomic DNA. Translation: AAP84348.1. BT007267 mRNA. Translation: AAP35931.1. BC004545 mRNA. Translation: AAH04545.1.
IPI	IPI00019382.
RefSeq	NP_001137391.1. NM_001143919.2. NP_858043.1. NM_181657.3.
UniGene	Hs.567248.
3D structure databases
ProteinModelPortal	Q15722.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15722. 1 interaction.
STRING	9606.ENSP00000307445.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q15722.
Polymorphism databases
DMDM	3041713.
Proteomic databases
PaxDb	Q15722.
PRIDE	Q15722.
Protocols and materials databases
DNASU	1241.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000345363; ENSP00000307445; ENSG00000213903. ENST00000396782; ENSP00000380002; ENSG00000213903. ENST00000396789; ENSP00000380008; ENSG00000213903.
GeneID	1241.
KEGG	hsa:1241.
UCSC	uc001wos.3. human.
Organism-specific databases
CTD	1241.
GeneCards	GC14P024780.
H-InvDB	HIX0172389.
HGNC	HGNC:6713. LTB4R.
HPA	HPA003873.
MIM	601531. gene.
neXtProt	NX_Q15722.
PharmGKB	PA30476.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG329990.
HOGENOM	HOG000008594.
HOVERGEN	HBG104134.
InParanoid	Q15722.
KO	K04296.
OMA	PGNSFVV.
OrthoDB	EOG4RV2SB.
PhylomeDB	Q15722.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q15722.
Bgee	Q15722.
CleanEx	HS_LTB4R.
Genevestigator	Q15722.
GermOnline	ENSG00000196943. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR003981. Leukotriene_B4_rcpt. IPR003983. Leukotriene_B4_typ-1_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01477. LTB1RECEPTOR. PR01476. LTBRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q15722.
ChEMBL	CHEMBL3911.
ChiTaRS	LTB4R. human.
GenomeRNAi	1241.
NextBio	5065.
SOURCE	Search...

Entry information

Entry name

LT4R1_HUMAN

Accession

Primary (citable) accession number: Q15722
Secondary accession number(s): Q13305 , Q53XV5, Q92641, Q9BSU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.