Reviewed, UniProtKB/Swiss-Prot Q15722 (LT4R1_HUMAN)

Names and origin

Protein names

Recommended name:
    Leukotriene B4 receptor 1
      Short name=LTB4-R 1
Alternative name(s):
    P2Y purinoceptor 7
      Short name=P2Y7
    Chemoattractant receptor-like 1
    G-protein coupled receptor 16

Gene names

Name:	LTB4R
Synonyms:	BLT, BLT1, BLTR, CMKRL1, GPR16, P2RY7

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Expressed at highest levels in heart, skeletal muscle and at lower levels in brain and liver. High level of expression in lymphoid tissues.
Post-translational modification	Phosphorylated by GRK6 upon leukotriene B4 binding; which promotes desensitization.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to IP3 second messenger (phospholipase C activating) Ref.1 Traceable author statement. Source: ProtInc cell motion Ref.3 Traceable author statement. Source: ProtInc immune response Ref.3 Traceable author statement. Source: ProtInc inflammatory response Ref.3 Traceable author statement. Source: ProtInc muscle contraction Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Ref.3 Traceable author statement. Source: ProtInc
Molecular function	nucleotide binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Leukotriene B4 receptor 1		PRO_0000069708
Regions
	Topological domain	1 – 19	19	Extracellular Potential
	Transmembrane	20 – 42	23	1 Potential
	Topological domain	43 – 54	12	Cytoplasmic Potential
	Transmembrane	55 – 75	21	2 Potential
	Topological domain	76 – 91	16	Extracellular Potential
	Transmembrane	92 – 113	22	3 Potential
	Topological domain	114 – 138	25	Cytoplasmic Potential
	Transmembrane	139 – 159	21	4 Potential
	Topological domain	160 – 178	19	Extracellular Potential
	Transmembrane	179 – 199	21	5 Potential
	Topological domain	200 – 221	22	Cytoplasmic Potential
	Transmembrane	222 – 242	21	6 Potential
	Topological domain	243 – 268	26	Extracellular Potential
	Transmembrane	269 – 289	21	7 Potential
	Topological domain	290 – 352	63	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	308	1	T → P or A: No effect on affinity for leukotriene B4, induces resistance to desensitization by GRK6, but minor effect on phosphorylation by GRK6
	Mutagenesis	310	1	S → A: No effect on affinity for leukotriene B4 or on desensitization by GRK6
	Sequence conflict	246	1	G → R in CAA67001. Ref.4
	Sequence conflict	272	1	A → V in AAC50628. Ref.1
	Sequence conflict	293	1	L → V in AAC50628. Ref.1
	Sequence conflict	346	1	L → F in AAH04545. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q15722-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 5A7BFC0A659AC81C	FASTA	352	37,557
	10 20 30 40 50 60 MNTTSSAAPP SLGVEFISLL AIILLSVALA VGLPGNSFVV WSILKRMQKR SVTALMVLNL 70 80 90 100 110 120 ALADLAVLLT APFFLHFLAQ GTWSFGLAGC RLCHYVCGVS MYASVLLITA MSLDRSLAVA 130 140 150 160 170 180 RPFVSQKLRT KAMARRVLAG IWVLSFLLAT PVLAYRTVVP WKTNMSLCFP RYPSEGHRAF 190 200 210 220 230 240 HLIFEAVTGF LLPFLAVVAS YSDIGRRLQA RRFRRSRRTG RLVVLIILTF AAFWLPYHVV 250 260 270 280 290 300 NLAEAGRALA GQAAGLGLVG KRLSLARNVL IALAFLSSSV NPVLYACAGG GLLRSAGVGF 310 320 330 340 350 VAKLLEGTGS EASSTRRGGS LGQTARSGPA ALEPGPSESL TASSPLKLNE LN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel P2 purinoceptor from human erythroleukemia cells." Akbar G.K.M., Dasari V.R., Webb T., Ayyanathan K., Pillarisetti K., Sandhu A.K., Athwal R.S., Daniel J.L., Ashby B., Barnard E.A., Kunapuli S.P. J. Biol. Chem. 271:18363-18367(1996) [PubMed: 8702478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"New members of the chemokine receptor gene family." Raport C.J., Schweickart V.L., Chantry D., Eddy R.L. Jr., Shows T.B., Godiska R., Gray P.W. J. Leukoc. Biol. 59:18-23(1996) [PubMed: 8558062] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis." Yokomizo T., Izumi T., Chang K., Takuwa Y., Shimizu T. Nature 387:620-624(1997) [PubMed: 9177352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of cDNA encoding a putative chemoattractant receptor." Owman C.S.O., Nilsson C., Lolait S.J. Genomics 37:187-194(1996) [PubMed: 8921391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cell-specific transcriptional regulation of human leukotriene B(4) receptor gene." Kato K., Yokomizo T., Izumi T., Shimizu T. J. Exp. Med. 192:413-420(2000) [PubMed: 10934229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Isolation of complete coding sequence for leukotriene B4 receptor (LTB4R)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization." Gaudreau R., Le Gouill C., Venne M.-H., Stankova J., Rola-Pleszczynski M. J. Biol. Chem. 277:31567-31576(2002) [PubMed: 12077128] [Abstract] Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-308 AND SER-310.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U41070 mRNA. Translation: AAC50628.1. U33448 Genomic DNA. Translation: AAB16747.1. Different initiation. D89079 mRNA. Translation: BAA20424.1. D89078 mRNA. Translation: BAA20423.1. X98356 mRNA. Translation: CAA67001.1. AB008193 Genomic DNA. Translation: BAB00611.1. AY322535 Genomic DNA. Translation: AAP84348.1. BC004545 mRNA. Translation: AAH04545.1.
RefSeq	NP_858043.1.
UniGene	Hs.655431
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q15722.
Genome annotation databases
Ensembl	ENSG00000213903. Homo sapiens. [Contig view]
GeneID	1241.
KEGG	hsa:1241.
Organism-specific databases
H-InvDB	HIX0011567.
HGNC	HGNC:6713. LTB4R.
HPA	HPA003873.
MIM	601531. gene.
PharmGKB	PA30476.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q15722.
Gene expression databases
ArrayExpress	Q15722.
CleanEx	HS_LTB4R.
GermOnline	ENSG00000196943. Homo sapiens.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR003981. LTB4_rcpt. IPR003983. LTB4_t1_rcpt. [Graphical view]
PANTHER	PTHR19264:SF245. LTB4_t1_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01477. LTB1RECEPTOR. PR01476. LTBRECEPTOR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	5065.
SOURCE	Search...

Entry information

Entry name

LT4R1_HUMAN

Accession

Primary (citable) accession number: Q15722
Secondary accession number(s): Q13305, Q92641, Q9BSU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	November 25, 2008
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)