Q15717 (ELAV1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ELAV-like protein 1

Alternative name(s):
Hu-antigen R
Short name=HuR

Gene names

Name:	ELAVL1
Synonyms:	HUR

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Ref.14
Subunit structure	Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ref.5 Ref.9 Ref.14
Subcellular location	Cytoplasm. Nucleus. Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2. Ref.7 Ref.14
Tissue specificity	Ubiquitous.
Post-translational modification	Phosphorylated by MAPKAPK2. Ref.7 Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. Ref.6
Sequence similarities	Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Ligand	RNA-binding
PTM	Acetylation Methylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	3'-UTR-mediated mRNA stabilization Inferred from direct assay Ref.7. Source: UniProtKB gene expression Traceable author statement. Source: Reactome mRNA metabolic process Traceable author statement. Source: Reactome multicellular organismal development Traceable author statement PubMed 9003489. Source: ProtInc positive regulation of translation Inferred from direct assay PubMed 21613615. Source: MGI
Cellular_component	cytosol Traceable author statement. Source: Reactome nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	mRNA 3'-UTR AU-rich region binding Inferred from direct assay PubMed 23056314. Source: MGI nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.18

Chain

2 – 326

325

ELAV-like protein 1

PRO_0000081577

Regions

Domain

20 – 98

79

RRM 1

Domain

106 – 186

81

RRM 2

Domain

244 – 322

79

RRM 3

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.18

Modified residue

2

1

Phosphoserine Ref.18

Modified residue

202

1

Phosphoserine Ref.12 Ref.15 Ref.16 Ref.18

Modified residue

217

1

Omega-N-methylated arginine; by CARM1 Ref.6

Experimental info

Sequence conflict

180

1

T → A in AAB41913. Ref.1

Secondary structure

1

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326

Helix Strand Turn

Details...

Beta strand

20 – 26

7

Helix

33 – 41

9

Beta strand

46 – 53

8

Beta strand

55 – 57

3

Beta strand

60 – 70

11

Helix

71 – 81

11

Beta strand

92 – 95

4

Helix

101 – 103

3

Beta strand

107 – 111

5

Helix

119 – 126

8

Helix

127 – 129

3

Beta strand

132 – 139

8

Turn

141 – 143

3

Beta strand

146 – 156

11

Helix

157 – 167

11

Beta strand

180 – 183

4

Sequences

	Sequence		Length	Mass (Da)
	Q15717 [UniParc]. Last modified May 15, 2002. Version 2. Checksum: 0B86143805264DEF	FASTA	326	36,092
	10 20 30 40 50 60 MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS 70 80 90 100 110 120 LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS EVIKDANLYI SGLPRTMTQK 130 140 150 160 170 180 DVEDMFSRFG RIINSRVLVD QTTGLSRGVA FIRFDKRSEA EEAITSFNGH KPPGSSEPIT 190 200 210 220 230 240 VKFAANPNQN KNVALLSQLY HSPARRFGGP VHHQAQRFRF SPMGVDHMSG LSGVNVPGNA 250 260 270 280 290 300 SSGWCIFIYN LGQDADEGIL WQMFGPFGAV TNVKVIRDFN TNKCKGFGFV TMTNYEEAAM 310 320 AIASLNGYRL GDKILQVSFK TNKSHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein." Ma W.-J., Cheng S., Campbell C., Wright A., Furneaux H.M. J. Biol. Chem. 271:8144-8151(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[5]	"Delineation of mRNA export pathways by the use of cell-permeable peptides." Gallouzi I.-E., Steitz J.A. Science 294:1895-1901(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ANP32A.
[6]	"Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." Li H., Park S., Kilburn B., Jelinek M.A., Henschen-Edman A., Aswad D.W., Stallcup M.R., Laird-Offringa I.A. J. Biol. Chem. 277:44623-44630(2002) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ARG-217.
[7]	"Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2." Tran H., Maurer F., Nagamine Y. Mol. Cell. Biol. 23:7177-7188(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING, SUBCELLULAR LOCATION, PHOSPHORYLATION BY MAPKAPK2.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells." Hoeck J., Weinmann L., Ender C., Ruedel S., Kremmer E., Raabe M., Urlaub H., Meister G. EMBO Rep. 8:1052-1060(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EIF2C1 AND EIF2C2.
[10]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins." Ray D., Kazan H., Chan E.T., Pena Castillo L., Chaudhry S., Talukder S., Blencowe B.J., Morris Q., Hughes T.R. Nat. Biotechnol. 27:667-670(2009) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING.
[14]	"Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs." Weidensdorfer D., Stoehr N., Baude A., Lederer M., Koehn M., Schierhorn A., Buchmeier S., Wahle E., Huettelmaiery S. RNA 15:104-115(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A MRNP COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[15]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[16]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[17]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-202, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U38175 mRNA. Translation: AAB41913.1.
BT009793 mRNA. Translation: AAP88795.1.
CH471139 Genomic DNA. Translation: EAW68949.1.
BC003376 mRNA. Translation: AAH03376.1.

IPI

IPI00940851.

RefSeq

NP_001410.2. NM_001419.2.

UniGene

Hs.184492.
Hs.713744.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HI9	X-ray	2.00	A/B/C/D	18-99	[»]
4ED5	X-ray	2.00	A/B	18-186	[»]
4EGL	X-ray	2.90	A	18-186	[»]
4FXV	X-ray	1.90	A/B/C/D	20-99	[»]

ProteinModelPortal

Q15717.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15717. 21 interactions.

MINT

MINT-5001300.

STRING

9606.ENSP00000385269.

PTM databases

PhosphoSite

Q15717.

Polymorphism databases

DMDM

20981691.

Proteomic databases

PaxDb

Q15717.

PeptideAtlas

Q15717.

PRIDE

Q15717.

Protocols and materials databases

DNASU

1994.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000407627; ENSP00000385269; ENSG00000066044.
ENST00000596459; ENSP00000472197; ENSG00000066044.

GeneID

1994.

KEGG

hsa:1994.

UCSC

uc002mjb.3. human.

Organism-specific databases

CTD

1994.

GeneCards

GC19M008023.

HGNC

HGNC:3312. ELAVL1.

HPA

CAB005256.

MIM

603466. gene.

neXtProt

NX_Q15717.

PharmGKB

PA27740.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0724.

HOGENOM

HOG000231162.

HOVERGEN

HBG002295.

InParanoid

Q15717.

KO

K13088.

OrthoDB

EOG4Z8XWZ.

PhylomeDB

Q15717.

Enzyme and pathway databases

Reactome

REACT_21257. Metabolism of RNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q15717.

Bgee

Q15717.

CleanEx

HS_ELAVL1.

Genevestigator

Q15717.

GermOnline

ENSG00000066044. Homo sapiens.

Family and domain databases

Gene3D

3.30.70.330. 3 hits.

InterPro

IPR006548. ELAD_HUD_SF.
IPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]

Pfam

PF00076. RRM_1. 3 hits.
[Graphical view]

PRINTS

PR00961. HUDSXLRNA.

SMART

SM00360. RRM. 3 hits.
[Graphical view]

TIGRFAMs

TIGR01661. ELAV_HUD_SF. 1 hit.

PROSITE

PS50102. RRM. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q15717.

ChEMBL

CHEMBL1250379.

EvolutionaryTrace

Q15717.

GenomeRNAi

1994.

NextBio

8063.

SOURCE

Search...

Entry information

Entry name

ELAV1_HUMAN

Accession

Primary (citable) accession number: Q15717
Secondary accession number(s): Q53XN6, Q9BTT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 15, 2002
Last modified:	May 1, 2013
This is version 136 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.