Q15717 (ELAV1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 136.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: ELAV-like protein 1 Alternative name(s): Hu-antigen R Short name=HuR | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 326 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. Ref.14 |
| Subunit structure | Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with ANP32A, EIF2C1 and EIF2C2. Ref.5 Ref.9 Ref.14 |
| Subcellular location | Cytoplasm. Nucleus. Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2. Ref.7 Ref.14 |
| Tissue specificity | Ubiquitous. |
| Post-translational modification | Phosphorylated by MAPKAPK2. Ref.7 Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. Ref.6 |
| Sequence similarities | Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Nucleus |
| Domain | Repeat |
| Ligand | RNA-binding |
| PTM | Acetylation Methylation Phosphoprotein |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | 3'-UTR-mediated mRNA stabilization Inferred from direct assay Ref.7. Source: UniProtKB gene expressionTraceable author statement. Source: Reactome mRNA metabolic processTraceable author statement. Source: Reactome multicellular organismal developmentTraceable author statement PubMed 9003489. Source: ProtInc positive regulation of translationInferred from direct assay PubMed 21613615. Source: MGI |
| Cellular_component | cytosol Traceable author statement. Source: Reactome nucleoplasmTraceable author statement. Source: Reactome |
| Molecular_function | mRNA 3'-UTR AU-rich region binding Inferred from direct assay PubMed 23056314. Source: MGI nucleotide bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.18 | |||||||||||||||||||||||||||||||||||
| Chain | 2 – 326 | 325 | ELAV-like protein 1 | PRO_0000081577 | ||||||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||||||
| Domain | 20 – 98 | 79 | RRM 1 | |||||||||||||||||||||||||||||||||||
| Domain | 106 – 186 | 81 | RRM 2 | |||||||||||||||||||||||||||||||||||
| Domain | 244 – 322 | 79 | RRM 3 | |||||||||||||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||||||||||||
| Modified residue | 2 | 1 | N-acetylserine Ref.18 | |||||||||||||||||||||||||||||||||||
| Modified residue | 2 | 1 | Phosphoserine Ref.18 | |||||||||||||||||||||||||||||||||||
| Modified residue | 202 | 1 | Phosphoserine Ref.12 Ref.15 Ref.16 Ref.18 | |||||||||||||||||||||||||||||||||||
| Modified residue | 217 | 1 | Omega-N-methylated arginine; by CARM1 Ref.6 | |||||||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||||||
| Sequence conflict | 180 | 1 | T → A in AAB41913. Ref.1 | |||||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||||
| Beta strand | 20 – 26 | 7 | ||||||||||||||||||||||||||||||||||||
| Helix | 33 – 41 | 9 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 46 – 53 | 8 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 55 – 57 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 60 – 70 | 11 | ||||||||||||||||||||||||||||||||||||
| Helix | 71 – 81 | 11 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 92 – 95 | 4 | ||||||||||||||||||||||||||||||||||||
| Helix | 101 – 103 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 107 – 111 | 5 | ||||||||||||||||||||||||||||||||||||
| Helix | 119 – 126 | 8 | ||||||||||||||||||||||||||||||||||||
| Helix | 127 – 129 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 132 – 139 | 8 | ||||||||||||||||||||||||||||||||||||
| Turn | 141 – 143 | 3 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 146 – 156 | 11 | ||||||||||||||||||||||||||||||||||||
| Helix | 157 – 167 | 11 | ||||||||||||||||||||||||||||||||||||
| Beta strand | 180 – 183 | 4 | ||||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein." Ma W.-J., Cheng S., Campbell C., Wright A., Furneaux H.M. J. Biol. Chem. 271:8144-8151(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix. |
| [5] | "Delineation of mRNA export pathways by the use of cell-permeable peptides." Gallouzi I.-E., Steitz J.A. Science 294:1895-1901(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ANP32A. |
| [6] | "Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." Li H., Park S., Kilburn B., Jelinek M.A., Henschen-Edman A., Aswad D.W., Stallcup M.R., Laird-Offringa I.A. J. Biol. Chem. 277:44623-44630(2002) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ARG-217. |
| [7] | "Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2." Tran H., Maurer F., Nagamine Y. Mol. Cell. Biol. 23:7177-7188(2003) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING, SUBCELLULAR LOCATION, PHOSPHORYLATION BY MAPKAPK2. |
| [8] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma. |
| [9] | "Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells." Hoeck J., Weinmann L., Ender C., Ruedel S., Kremmer E., Raabe M., Urlaub H., Meister G. EMBO Rep. 8:1052-1060(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EIF2C1 AND EIF2C2. |
| [10] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma. |
| [11] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma. |
| [12] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [13] | "Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins." Ray D., Kazan H., Chan E.T., Pena Castillo L., Chaudhry S., Talukder S., Blencowe B.J., Morris Q., Hughes T.R. Nat. Biotechnol. 27:667-670(2009) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING. |
| [14] | "Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs." Weidensdorfer D., Stoehr N., Baude A., Lederer M., Koehn M., Schierhorn A., Buchmeier S., Wahle E., Huettelmaiery S. RNA 15:104-115(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A MRNP COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY. |
| [15] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Leukemic T-cell. |
| [16] | "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma. |
| [17] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| [18] | "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-202, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | U38175 mRNA. Translation: AAB41913.1. BT009793 mRNA. Translation: AAP88795.1. CH471139 Genomic DNA. Translation: EAW68949.1. BC003376 mRNA. Translation: AAH03376.1. | ||||||||||||||||||||||||||||||
| IPI | IPI00940851. | ||||||||||||||||||||||||||||||
| RefSeq | NP_001410.2. NM_001419.2. | ||||||||||||||||||||||||||||||
| UniGene | Hs.184492. Hs.713744. | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||||||||||||||||||||
| ProteinModelPortal | Q15717. | ||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| IntAct | Q15717. 21 interactions. | ||||||||||||||||||||||||||||||
| MINT | MINT-5001300. | ||||||||||||||||||||||||||||||
| STRING | 9606.ENSP00000385269. | ||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||
| PhosphoSite | Q15717. | ||||||||||||||||||||||||||||||
Polymorphism databases | |||||||||||||||||||||||||||||||
| DMDM | 20981691. | ||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||
| PaxDb | Q15717. | ||||||||||||||||||||||||||||||
| PeptideAtlas | Q15717. | ||||||||||||||||||||||||||||||
| PRIDE | Q15717. | ||||||||||||||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||||||||||||||
| DNASU | 1994. | ||||||||||||||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENST00000407627; ENSP00000385269; ENSG00000066044. ENST00000596459; ENSP00000472197; ENSG00000066044. | ||||||||||||||||||||||||||||||
| GeneID | 1994. | ||||||||||||||||||||||||||||||
| KEGG | hsa:1994. | ||||||||||||||||||||||||||||||
| UCSC | uc002mjb.3. human. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| CTD | 1994. | ||||||||||||||||||||||||||||||
| GeneCards | GC19M008023. | ||||||||||||||||||||||||||||||
| HGNC | HGNC:3312. ELAVL1. | ||||||||||||||||||||||||||||||
| HPA | CAB005256. | ||||||||||||||||||||||||||||||
| MIM | 603466. gene. | ||||||||||||||||||||||||||||||
| neXtProt | NX_Q15717. | ||||||||||||||||||||||||||||||
| PharmGKB | PA27740. | ||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| eggNOG | COG0724. | ||||||||||||||||||||||||||||||
| HOGENOM | HOG000231162. | ||||||||||||||||||||||||||||||
| HOVERGEN | HBG002295. | ||||||||||||||||||||||||||||||
| InParanoid | Q15717. | ||||||||||||||||||||||||||||||
| KO | K13088. | ||||||||||||||||||||||||||||||
| OrthoDB | EOG4Z8XWZ. | ||||||||||||||||||||||||||||||
| PhylomeDB | Q15717. | ||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||
| Reactome | REACT_21257. Metabolism of RNA. REACT_71. Gene Expression. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | Q15717. | ||||||||||||||||||||||||||||||
| Bgee | Q15717. | ||||||||||||||||||||||||||||||
| CleanEx | HS_ELAVL1. | ||||||||||||||||||||||||||||||
| Genevestigator | Q15717. | ||||||||||||||||||||||||||||||
| GermOnline | ENSG00000066044. Homo sapiens. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| Gene3D | 3.30.70.330. 3 hits. | ||||||||||||||||||||||||||||||
| InterPro | IPR006548. ELAD_HUD_SF. IPR002343. Hud_Sxl_RNA. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view] | ||||||||||||||||||||||||||||||
| Pfam | PF00076. RRM_1. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR00961. HUDSXLRNA. | ||||||||||||||||||||||||||||||
| SMART | SM00360. RRM. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| TIGRFAMs | TIGR01661. ELAV_HUD_SF. 1 hit. | ||||||||||||||||||||||||||||||
| PROSITE | PS50102. RRM. 3 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Other | |||||||||||||||||||||||||||||||
| BindingDB | Q15717. | ||||||||||||||||||||||||||||||
| ChEMBL | CHEMBL1250379. | ||||||||||||||||||||||||||||||
| EvolutionaryTrace | Q15717. | ||||||||||||||||||||||||||||||
| GenomeRNAi | 1994. | ||||||||||||||||||||||||||||||
| NextBio | 8063. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | ELAV1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15717 Secondary accession number(s): Q53XN6, Q9BTT1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
