Reviewed, UniProtKB/Swiss-Prot Q15717 (ELAV1_HUMAN)

Names and origin

Protein names

Recommended name:
    ELAV-like protein 1
Alternative name(s):
    Hu-antigen R
      Short name=HuR

Gene names

Name:	ELAVL1
Synonyms:	HUR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs.
Subunit structure	Interacts with ANP32A. Ref.3
Tissue specificity	Ubiquitous.
Post-translational modification	Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. Ref.4
Sequence similarities	Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Domain	Repeat
Ligand	RNA-binding
PTM	Methylation Phosphoprotein
Gene Ontology (GO)
Biological process	multicellular organismal development Traceable author statement. Source: ProtInc
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct mRNA binding Traceable author statement. Source: ProtInc nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-374260,EBI-374260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	ELAV-like protein 1		PRO_0000081577
Regions
	Domain	20 – 98	79	RRM 1
	Domain	106 – 186	81	RRM 2
	Domain	244 – 322	79	RRM 3
Amino acid modifications
	Modified residue	202	1	Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8
	Modified residue	217	1	Omega-N-methylated arginine; by CARM1 Ref.4
Experimental info
	Sequence conflict	180	1	T → A in AAB41913. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q15717-1 [UniParc]. Last modified May 15, 2002. Version 2. Checksum: 0B86143805264DEF	FASTA	326	36,092
	10 20 30 40 50 60 MSNGYEDHMA EDCRGDIGRT NLIVNYLPQN MTQDELRSLF SSIGEVESAK LIRDKVAGHS 70 80 90 100 110 120 LGYGFVNYVT AKDAERAINT LNGLRLQSKT IKVSYARPSS EVIKDANLYI SGLPRTMTQK 130 140 150 160 170 180 DVEDMFSRFG RIINSRVLVD QTTGLSRGVA FIRFDKRSEA EEAITSFNGH KPPGSSEPIT 190 200 210 220 230 240 VKFAANPNQN KNVALLSQLY HSPARRFGGP VHHQAQRFRF SPMGVDHMSG LSGVNVPGNA 250 260 270 280 290 300 SSGWCIFIYN LGQDADEGIL WQMFGPFGAV TNVKVIRDFN TNKCKGFGFV TMTNYEEAAM 310 320 AIASLNGYRL GDKILQVSFK TNKSHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein." Ma W.-J., Cheng S., Campbell C., Wright A., Furneaux H.M. J. Biol. Chem. 271:8144-8151(1996) [PubMed: 8626503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix.
[3]	"Delineation of mRNA export pathways by the use of cell-permeable peptides." Gallouzi I.-E., Steitz J.A. Science 294:1895-1901(2001) [PubMed: 11729309] [Abstract] Cited for: INTERACTION WITH ANP32A.
[4]	"Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase." Li H., Park S., Kilburn B., Jelinek M.A., Henschen-Edman A., Aswad D.W., Stallcup M.R., Laird-Offringa I.A. J. Biol. Chem. 277:44623-44630(2002) [PubMed: 12237300] [Abstract] Cited for: METHYLATION AT ARG-217.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY.
[9]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38175 mRNA. Translation: AAB41913.1. BC003376 mRNA. Translation: AAH03376.1.
IPI	IPI00301936.
RefSeq	NP_001410.2.
UniGene	Hs.184492 Hs.713744
3D structure databases
HSSP	HSSP built from PDB template 1FXL based on UniProtKB P26378.
SMR	Q15717. Positions 19-184.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15717. 3 interactions.
PTM databases
PhosphoSite	Q15717.
Proteomic databases
PeptideAtlas	Q15717.
PRIDE	Q15717.
Genome annotation databases
Ensembl	ENSG00000066044. Homo sapiens. [Contig view]
GeneID	1994.
KEGG	hsa:1994.
Organism-specific databases
GeneCards	GC19M007929.
H-InvDB	HIX0017616.
HGNC	HGNC:3312. ELAVL1.
HPA	CAB005256.
MIM	603466. gene.
PharmGKB	PA27740.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q15717.
HOVERGEN	Q15717.
OMA	Q15717. SFNGLRL.
Gene expression databases
ArrayExpress	Q15717.
Bgee	Q15717.
CleanEx	HS_ELAVL1.
GermOnline	ENSG00000066044. Homo sapiens.
Family and domain databases
InterPro	IPR012677. a_b_plait_nuc_bd. IPR006548. ELAD_HUD_SF. IPR002343. Hud_Sxl_RNA. IPR000504. RRM_RNP1. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 3 hits.
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
PRINTS	PR00961. HUDSXLRNA.
SMART	SM00360. RRM. 3 hits. [Graphical view]
TIGRFAMs	TIGR01661. ELAV_HUD_SF. 1 hit.
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	8063.
SOURCE	Search...

Entry information

Entry name

ELAV1_HUMAN

Accession

Primary (citable) accession number: Q15717
Secondary accession number(s): Q9BTT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 15, 2002
Last modified:	June 16, 2009
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)