Q15697 (ZN174_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein 174

Alternative name(s):
AW-1
Zinc finger and SCAN domain-containing protein 8

Gene names

Name:	ZNF174
Synonyms:	ZSCAN8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor. Ref.1
Subunit structure	Homodimer. Ref.4
Subcellular location	Nucleus.
Tissue specificity	Expressed in a variety of organs, but most strongly in adult testis and ovary followed by small intestine, colon, prostate, thymus, spleen, pancreas, skeletal muscle, heart, brain and kidney. Also expressed in umbilical vein endothelial cells, foreskin fibroblast and Hep-G2 cells.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. Contains 1 SCAN box domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: UniProtKB viral reproduction Inferred from electronic annotation. Source: InterPro
Cellular_component	actin cytoskeleton Inferred from direct assay. Source: HPA cytoplasm Inferred from direct assay. Source: HPA nucleus Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome transcription regulatory region DNA binding Inferred from direct assay Ref.1. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15697-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15697-2)

The sequence of this isoform differs from the canonical sequence as follows:
     210-234: APRMRSDNKENPQQEGAKGAKPCAV → LLIEKTDPNMATDELPCKLWLSFIA
     235-407: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 407

407

Zinc finger protein 174

PRO_0000047439

Regions

Domain

59 – 124

66

SCAN box

Zinc finger

326 – 348

23

C2H2-type 1

Zinc finger

354 – 376

23

C2H2-type 2

Zinc finger

382 – 405

24

C2H2-type 3

Natural variations

Alternative sequence

210 – 234

25

APRMR…KPCAV → LLIEKTDPNMATDELPCKLW LSFIA in isoform 2.

VSP_006897

Alternative sequence

235 – 407

173

Missing in isoform 2.

VSP_006898

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

407

Helix Strand Turn

Details...

Beta strand

41 – 43

3

Helix

44 – 50

7

Helix

61 – 75

15

Helix

82 – 97

16

Helix

101 – 107

7

Helix

114 – 125

12

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1FAB4A631EA2ABCC	FASTA	407	46,455
	10 20 30 40 50 60 MAAKMEITLS SNTEASSKQE RHIIAKLEEK RGPPLQKNCP DPELCRQSFR RFCYQEVSGP 70 80 90 100 110 120 QEALSQLRQL CRQWLQPELH TKEQILELLV MEQFLTILPP EIQARVRHRC PMSSKEIVTL 130 140 150 160 170 180 VEDFHRASKK PKQWVAVCMQ GQKVLLEKTG SQLGEQELPD FQPQTPRRDL RESSPAEPSQ 190 200 210 220 230 240 AGAYDRLSPH HWEKSPLLQE PTPKLAGTEA PRMRSDNKEN PQQEGAKGAK PCAVSAGRSK 250 260 270 280 290 300 GNGLQNPEPR GANMSEPRLS RRQVSSPNAQ KPFAHYQRHC RVEYISSPLK SHPLRELKKS 310 320 330 340 350 360 KGGKRSLSNR LQHLGHQPTR SAKKPYKCDD CGKSFTWNSE LKRHKRVHTG ERPYTCGECG 370 380 390 400 NCFGRQSTLK LHQRIHTGEK PYQCGQCGKS FRQSSNLHQH HRLHHGD
	Isoform 2 [UniParc]. Checksum: A2A40D0A1AA96BC6	FASTA	234	26,884
	10 20 30 40 50 60 MAAKMEITLS SNTEASSKQE RHIIAKLEEK RGPPLQKNCP DPELCRQSFR RFCYQEVSGP 70 80 90 100 110 120 QEALSQLRQL CRQWLQPELH TKEQILELLV MEQFLTILPP EIQARVRHRC PMSSKEIVTL 130 140 150 160 170 180 VEDFHRASKK PKQWVAVCMQ GQKVLLEKTG SQLGEQELPD FQPQTPRRDL RESSPAEPSQ 190 200 210 220 230 AGAYDRLSPH HWEKSPLLQE PTPKLAGTEL LIEKTDPNMA TDELPCKLWL SFIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a novel zinc-finger protein with transcription repressor activity." Williams A.J., Khachigian L.M., Shows T., Collins T. J. Biol. Chem. 270:22143-22152(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Cervix and Lung.
[4]	"Mammalian SCAN domain dimer is a domain-swapped homolog of the HIV capsid C-terminal domain." Ivanov D., Stone J.R., Maki J.L., Collins T., Wagner G. Mol. Cell 17:137-143(2005) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 37-132, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U31248 mRNA. Translation: AAB04897.1.
BT006923 mRNA. Translation: AAP35569.1.
BC000876 mRNA. Translation: AAH00876.1.
BC001161 mRNA. Translation: AAH01161.1.

IPI

IPI00018955.
IPI00029669.

PIR

I39152.

RefSeq

NP_001027463.1. NM_001032292.2.
NP_003441.1. NM_003450.2.

UniGene

Hs.155204.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y7Q	NMR	-	A/B	37-132	[»]

ProteinModelPortal

Q15697.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15697. 2 interactions.

STRING

9606.ENSP00000268655.

PTM databases

PhosphoSite

Q15697.

Polymorphism databases

DMDM

3123173.

Proteomic databases

PaxDb

Q15697.

PRIDE

Q15697.

Protocols and materials databases

DNASU

7727.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000268655; ENSP00000268655; ENSG00000103343.
ENST00000344823; ENSP00000339781; ENSG00000103343.
ENST00000571936; ENSP00000460397; ENSG00000103343.
ENST00000575752; ENSP00000461502; ENSG00000103343.

GeneID

7727.

KEGG

hsa:7727.

UCSC

uc002cva.2. human.
uc002cvb.3. human.

Organism-specific databases

CTD

7727.

GeneCards

GC16P003451.

HGNC

HGNC:12963. ZNF174.

HPA

HPA009656.

MIM

603900. gene.

neXtProt

NX_Q15697.

PharmGKB

PA37545.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5048.

HOGENOM

HOG000234619.

HOVERGEN

HBG018163.

InParanoid

Q15697.

KO

K09230.

OMA

FAHYQRH.

OrthoDB

EOG4QRH42.

Gene expression databases

ArrayExpress

Q15697.

Bgee

Q15697.

CleanEx

HS_ZNF174.

Genevestigator

Q15697.

GermOnline

ENSG00000103343. Homo sapiens.

Family and domain databases

Gene3D

3.30.160.60. 3 hits.

InterPro

IPR008916. Retrov_capsid_C.
IPR003309. Tscrpt_reg_SCAN.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

Pfam

PF02023. SCAN. 1 hit.
[Graphical view]

SMART

SM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]

SUPFAM

SSF47353. Retrov_capsid_C. 1 hit.

PROSITE

PS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ZNF174. human.

EvolutionaryTrace

Q15697.

GenomeRNAi

7727.

NextBio

29924.

SOURCE

Search...

Entry information

Entry name

ZN174_HUMAN

Accession

Primary (citable) accession number: Q15697
Secondary accession number(s): Q53Y68, Q9BQ34

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.