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Q15696

- U2AFM_HUMAN

UniProt

Q15696 - U2AFM_HUMAN

Protein

U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2

Gene

ZRSR2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (01 May 1997)
      Previous versions | rss
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    Functioni

    Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri166 – 19429C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri306 – 33328C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nucleotide binding Source: InterPro
    3. pre-mRNA 3'-splice site binding Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB
    2. RNA splicing Source: HGNC
    3. spliceosomal complex assembly Source: UniProtKB

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
    Alternative name(s):
    CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2
    Renal carcinoma antigen NY-REN-20
    U2(RNU2) small nuclear RNA auxiliary factor 1-like 2
    U2AF35-related protein
    Short name:
    URP
    Gene namesi
    Name:ZRSR2
    Synonyms:U2AF1-RS2, U2AF1L2, U2AF1RS2, URP
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome X

    Organism-specific databases

    HGNCiHGNC:23019. ZRSR2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. U12-type spliceosomal complex Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162410930.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 482482U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2PRO_0000082001Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei349 – 3491Phosphoserine3 Publications
    Modified residuei384 – 3841Phosphoserine3 Publications

    Post-translational modificationi

    Phosphorylated in the RS domain by SRPK1.5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ15696.
    PaxDbiQ15696.
    PRIDEiQ15696.

    PTM databases

    PhosphoSiteiQ15696.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Gene expression databases

    ArrayExpressiQ15696.
    BgeeiQ15696.
    CleanExiHS_ZRSR2.
    GenevestigatoriQ15696.

    Organism-specific databases

    HPAiHPA047500.

    Interactioni

    Subunit structurei

    Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts (via RS domain) with SRSF1 and SRSF2. Interacts with U2AF2/U2AF65.3 Publications

    Protein-protein interaction databases

    BioGridi113865. 5 interactions.
    IntActiQ15696. 2 interactions.
    STRINGi9606.ENSP00000303015.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15696.
    SMRiQ15696. Positions 228-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini198 – 304107RRMPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi46 – 494Poly-Glu
    Compositional biasi118 – 1236Poly-Glu
    Compositional biasi414 – 46956Arg/Ser-rich (RS domain)Add
    BLAST

    Sequence similaritiesi

    Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation
    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri166 – 19429C3H1-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri306 – 33328C3H1-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG304335.
    HOGENOMiHOG000049279.
    HOVERGENiHBG054605.
    InParanoidiQ15696.
    OMAiERMGHHD.
    PhylomeDBiQ15696.
    TreeFamiTF324447.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR009145. U2_small.
    IPR000571. Znf_CCCH.
    [Graphical view]
    PANTHERiPTHR12620. PTHR12620. 1 hit.
    PfamiPF00642. zf-CCCH. 1 hit.
    [Graphical view]
    PRINTSiPR01848. U2AUXFACTOR.
    SMARTiSM00360. RRM. 1 hit.
    SM00356. ZnF_C3H1. 2 hits.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    PS50103. ZF_C3H1. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q15696-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPEKMTFP EKPSHKKYRA ALKKEKRKKR RQELARLRDS GLSQKEEEED    50
    TFIEEQQLEE EKLLERERQR LHEEWLLREQ KAQEEFRIKK EKEEAAKKRQ 100
    EEQERKLKEQ WEEQQRKERE EEEQKRQEKK EKEEALQKML DQAENELENG 150
    TTWQNPEPPV DFRVMEKDRA NCPFYSKTGA CRFGDRCSRK HNFPTSSPTL 200
    LIKSMFTTFG MEQCRRDDYD PDASLEYSEE ETYQQFLDFY EDVLPEFKNV 250
    GKVIQFKVSC NLEPHLRGNV YVQYQSEEEC QAALSLFNGR WYAGRQLQCE 300
    FCPVTRWKMA ICGLFEIQQC PRGKHCNFLH VFRNPNNEFW EANRDIYLSP 350
    DRTGSSFGKN SERRERMGHH DDYYSRLRGR RNPSPDHSYK RNGESERKSS 400
    RHRGKKSHKR TSKSRERHNS RSRGRNRDRS RDRSRGRGSR SRSRSRSRRS 450
    RRSRSQSSSR SRSRGRRRSG NRDRTVQSPK SK 482
    Length:482
    Mass (Da):58,045
    Last modified:May 1, 1997 - v2
    Checksum:i1DACC8A6CA4727A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49677 mRNA. Translation: BAA08533.1.
    AC004106 Genomic DNA. No translation available.
    AC096510 Genomic DNA. No translation available.
    BC113454 mRNA. Translation: AAI13455.1.
    BC113480 mRNA. Translation: AAI13481.1.
    CCDSiCCDS14172.1.
    RefSeqiNP_005080.1. NM_005089.3.
    UniGeneiHs.171909.

    Genome annotation databases

    EnsembliENST00000307771; ENSP00000303015; ENSG00000169249.
    GeneIDi8233.
    KEGGihsa:8233.
    UCSCiuc004cxg.4. human.

    Polymorphism databases

    DMDMi2833266.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D49677 mRNA. Translation: BAA08533.1 .
    AC004106 Genomic DNA. No translation available.
    AC096510 Genomic DNA. No translation available.
    BC113454 mRNA. Translation: AAI13455.1 .
    BC113480 mRNA. Translation: AAI13481.1 .
    CCDSi CCDS14172.1.
    RefSeqi NP_005080.1. NM_005089.3.
    UniGenei Hs.171909.

    3D structure databases

    ProteinModelPortali Q15696.
    SMRi Q15696. Positions 228-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113865. 5 interactions.
    IntActi Q15696. 2 interactions.
    STRINGi 9606.ENSP00000303015.

    PTM databases

    PhosphoSitei Q15696.

    Polymorphism databases

    DMDMi 2833266.

    Proteomic databases

    MaxQBi Q15696.
    PaxDbi Q15696.
    PRIDEi Q15696.

    Protocols and materials databases

    DNASUi 8233.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307771 ; ENSP00000303015 ; ENSG00000169249 .
    GeneIDi 8233.
    KEGGi hsa:8233.
    UCSCi uc004cxg.4. human.

    Organism-specific databases

    CTDi 8233.
    GeneCardsi GC0XP015808.
    H-InvDB HIX0017758.
    HGNCi HGNC:23019. ZRSR2.
    HPAi HPA047500.
    MIMi 300028. gene.
    neXtProti NX_Q15696.
    PharmGKBi PA162410930.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG304335.
    HOGENOMi HOG000049279.
    HOVERGENi HBG054605.
    InParanoidi Q15696.
    OMAi ERMGHHD.
    PhylomeDBi Q15696.
    TreeFami TF324447.

    Miscellaneous databases

    ChiTaRSi ZRSR2. human.
    GeneWikii ZRSR2.
    GenomeRNAii 8233.
    NextBioi 30981.
    PROi Q15696.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15696.
    Bgeei Q15696.
    CleanExi HS_ZRSR2.
    Genevestigatori Q15696.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR009145. U2_small.
    IPR000571. Znf_CCCH.
    [Graphical view ]
    PANTHERi PTHR12620. PTHR12620. 1 hit.
    Pfami PF00642. zf-CCCH. 1 hit.
    [Graphical view ]
    PRINTSi PR01848. U2AUXFACTOR.
    SMARTi SM00360. RRM. 1 hit.
    SM00356. ZnF_C3H1. 2 hits.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    PS50103. ZF_C3H1. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and mapping of human homologues of an imprinted mouse gene U2af1-rs1."
      Kitagawa K., Wang X., Hatada I., Yamaoka T., Nojima H., Inazawa J., Abe T., Mitsuya K., Oshimura M., Murata A., Monden M., Mukai T.
      Genomics 30:257-263(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The DNA sequence of the human X chromosome."
      Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
      , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
      Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA."
      Tronchere H., Wang J., Fu X.D.
      Nature 388:397-400(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION, INTERACTION WITH SRSF1; SRSF2; SRPK1 AND U2AF2.
    5. Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN.
      Tissue: Renal cell carcinoma.
    6. "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome."
      Will C.L., Schneider C., Hossbach M., Urlaub H., Rauhut R., Elbashir S., Tuschl T., Luehrmann R.
      RNA 10:929-941(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE U11/U12 SPLICEOSOME COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing."
      Shen H., Zheng X., Luecke S., Green M.R.
      Genes Dev. 24:2389-2394(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE U11/U12 SPLICEOSOME COMPLEX, RNA-BINDING.
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiU2AFM_HUMAN
    AccessioniPrimary (citable) accession number: Q15696
    Secondary accession number(s): Q14D69
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3