ID   U2AFL_HUMAN             Reviewed;         479 AA.
AC   Q15695; B2R901; Q13570; Q2M3R8;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   24-JAN-2024, entry version 172.
DE   RecName: Full=U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 1 {ECO:0000305};
DE   AltName: Full=CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 1;
DE   AltName: Full=U2(RNU2) small nuclear RNA auxiliary factor 1-like 1;
GN   Name=ZRSR2P1 {ECO:0000312|HGNC:HGNC:12456};
GN   Synonyms=U2AF1-RS1, U2AF1L1, U2AF1P, U2AF1RS1, U2AFBPL, ZRSR1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=8586425; DOI=10.1006/geno.1995.9879;
RA   Kitagawa K., Wang X., Hatada I., Yamaoka T., Nojima H., Inazawa J., Abe T.,
RA   Mitsuya K., Oshimura M., Murata A., Monden M., Mukai T.;
RT   "Isolation and mapping of human homologues of an imprinted mouse gene
RT   U2af1-rs1.";
RL   Genomics 30:257-263(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8630064; DOI=10.1006/bbrc.1996.0716;
RA   Pearsall R.S., Shibata H., Brozowska A., Yoshino K., Okuda K., Dejong P.J.,
RA   Plass C., Chapman V.M., Hayashizaki Y., Held W.A.;
RT   "Absence of imprinting in U2AFBPL, a human homologue of the imprinted mouse
RT   gene U2afbp-rs.";
RL   Biochem. Biophys. Res. Commun. 222:171-177(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Small intestine;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15372022; DOI=10.1038/nature02919;
RA   Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA   Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA   She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA   Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA   Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA   Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA   Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA   Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA   Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA   Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA   Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA   Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA   Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT   "The DNA sequence and comparative analysis of human chromosome 5.";
RL   Nature 431:268-274(2004).
CC   -!- INTERACTION:
CC       Q15695; O60333-2: KIF1B; NbExp=3; IntAct=EBI-12270264, EBI-10975473;
CC       Q15695; P55081: MFAP1; NbExp=3; IntAct=EBI-12270264, EBI-1048159;
CC       Q15695; Q6BDI9: REP15; NbExp=5; IntAct=EBI-12270264, EBI-12048237;
CC       Q15695; A7MD48: SRRM4; NbExp=3; IntAct=EBI-12270264, EBI-3867173;
CC       Q15695; P26368-2: U2AF2; NbExp=3; IntAct=EBI-12270264, EBI-11097439;
CC       Q15695; Q15696: ZRSR2; NbExp=4; IntAct=EBI-12270264, EBI-6657923;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- CAUTION: Defined as a pseudogene by HGNC. However, proteomics data
CC       suggest the existence of the protein. Appears to have arisen by
CC       retrotransposition of ZRSR2. It is uncertain if ZRSR2P1 is the ortholog
CC       of mouse ZRSR1, synteny is not conserved. {ECO:0000305}.
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DR   EMBL; D49676; BAA08532.1; -; mRNA.
DR   EMBL; U51224; AAA98669.1; -; Genomic_DNA.
DR   EMBL; AK313576; BAG36348.1; -; mRNA.
DR   EMBL; AC008536; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q15695; -.
DR   SMR; Q15695; -.
DR   IntAct; Q15695; 9.
DR   iPTMnet; Q15695; -.
DR   PhosphoSitePlus; Q15695; -.
DR   SwissPalm; Q15695; -.
DR   BioMuta; ZRSR1; -.
DR   DMDM; 2833265; -.
DR   jPOST; Q15695; -.
DR   MassIVE; Q15695; -.
DR   MaxQB; Q15695; -.
DR   PaxDb; 9606-ENSP00000375133; -.
DR   PeptideAtlas; Q15695; -.
DR   ProteomicsDB; 60703; -.
DR   Pumba; Q15695; -.
DR   UCSC; uc021ycm.2; human.
DR   AGR; HGNC:12456; -.
DR   GeneCards; ZRSR2P1; -.
DR   HGNC; HGNC:12456; ZRSR2P1.
DR   MIM; 601079; gene.
DR   neXtProt; NX_Q15695; -.
DR   eggNOG; KOG2202; Eukaryota.
DR   HOGENOM; CLU_029117_1_0_1; -.
DR   InParanoid; Q15695; -.
DR   PhylomeDB; Q15695; -.
DR   TreeFam; TF324447; -.
DR   PathwayCommons; Q15695; -.
DR   Pharos; Q15695; Tdark.
DR   PRO; PR:Q15695; -.
DR   Proteomes; UP000005640; Unplaced.
DR   RNAct; Q15695; Protein.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0089701; C:U2AF complex; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030628; F:pre-mRNA 3'-splice site binding; IBA:GO_Central.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   CDD; cd12540; RRM_U2AFBPL; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR003954; RRM_dom_euk.
DR   InterPro; IPR009145; U2AF_small.
DR   InterPro; IPR000571; Znf_CCCH.
DR   PANTHER; PTHR12620:SF33; U2 SMALL NUCLEAR RIBONUCLEOPROTEIN AUXILIARY FACTOR 35 KDA SUBUNIT-RELATED PROTEIN 1-RELATED; 1.
DR   PANTHER; PTHR12620; U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   Pfam; PF00642; zf-CCCH; 1.
DR   PRINTS; PR01848; U2AUXFACTOR.
DR   SMART; SM00361; RRM_1; 1.
DR   SMART; SM00356; ZnF_C3H1; 2.
DR   SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR   PROSITE; PS50102; RRM; 1.
DR   PROSITE; PS50103; ZF_C3H1; 2.
DR   Genevisible; Q15695; HS.
PE   1: Evidence at protein level;
KW   Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein;
KW   Reference proteome; Repeat; Ribonucleoprotein; RNA-binding;
KW   Ubl conjugation; Zinc; Zinc-finger.
FT   CHAIN           1..479
FT                   /note="U2 small nuclear ribonucleoprotein auxiliary factor
FT                   35 kDa subunit-related protein 1"
FT                   /id="PRO_0000081999"
FT   DOMAIN          203..309
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   ZN_FING         171..199
FT                   /note="C3H1-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT   ZN_FING         311..338
FT                   /note="C3H1-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT   REGION          1..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          356..479
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        363..405
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        406..429
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        444..466
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         354
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q15696"
FT   MOD_RES         389
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q15696"
FT   CROSSLNK        67
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2)"
FT                   /evidence="ECO:0000250|UniProtKB:Q15696"
FT   CONFLICT        135
FT                   /note="K -> E (in Ref. 3; BAG36348)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        195
FT                   /note="K -> R (in Ref. 3; BAG36348)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   479 AA;  57643 MW;  96F326694BD4E7C0 CRC64;
     MAALEKMTFP KKMTFPEKPS HKKYRAALKK EKRKKRRQEL ARLRDSGLSQ EEEEDTFIEE
     QQLEEEKLLE RERERLHEEW LLREQKAQEE FRIKKEKEEA AKKWLEEQER KLKEQWKEQQ
     RKEREEEEQK QQEKKEKEEA VQKMLDQAEN DLENSTTWQN PEPPVDFRVM EKDRANCPFY
     SKTGACRFGD RCSRKHNFPT SSPTLLIKSM FTTFGMEQCR RDDYDPDASL EYSEEETYQQ
     FLDFYEDVLP EFKNVGKVIQ FKVSCNLEPH LRGNVYVQYQ SEEECQAALS LFNGRWYAGR
     QLQCEFCPVT RWKMAICGLF EIQQCPRGKH CNFLHVFRNP NNEFWEANRD IYLSSDQTGS
     SFGKNSERRE KMGHHDHYYS RQRGRRNPSP DHTYKRNGES ERKKSSHRGK KSHKRTSKSR
     ERHNSPSRGR NRHRSWDQGR RSQSRRSHRS RSQSSSRCRS RGRRKSGNRD RTVQSPQSK
//