Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q15669

- RHOH_HUMAN

UniProt

Q15669 - RHOH_HUMAN

Protein

Rho-related GTP-binding protein RhoH

Gene

RHOH

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Negative regulator of hematopoietic progenitor cell proliferation, survival and migration. Critical regulator of thymocyte development and T-cell antigen receptor (TCR) signaling by mediating recruitment and activation of ZAP70. Required for phosphorylation of CD3Z, membrane translocation of ZAP70 and subsequent activation of the ZAP70-mediated pathways. Essential for efficient beta-selection and positive selection by promoting the ZAP70-dependent phosphorylation of the LAT signalosome during pre-TCR and TCR signaling. Crucial for thymocyte maturation during DN3 to DN4 transition and during positive selection. Plays critical roles in mast cell function by facilitating phosphorylation of SYK in Fc epsilon RI-mediated signal transduction. Essential for the phosphorylation of LAT, LCP2, PLCG1 and PLCG2 and for Ca2+ mobilization in mast cells By similarity. Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins. Inhibits the activation of NF-kappa-B by TNF and IKKB and the activation of CRK/p38 by TNF. Inhibits activities of RAC1, RHOA and CDC42. Negatively regulates leukotriene production in neutrophils.By similarity2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188GTPBy similarity
    Nucleotide bindingi58 – 625GTPBy similarity
    Nucleotide bindingi116 – 1194GTPBy similarity

    GO - Molecular functioni

    1. GTPase inhibitor activity Source: UniProtKB
    2. GTP binding Source: UniProtKB
    3. kinase inhibitor activity Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. Rho GTPase binding Source: UniProtKB

    GO - Biological processi

    1. mast cell activation Source: Ensembl
    2. negative regulation of catalytic activity Source: GOC
    3. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    4. negative regulation of phosphorylation Source: GOC
    5. regulation of small GTPase mediated signal transduction Source: Reactome
    6. regulation of transcription, DNA-templated Source: UniProtKB
    7. small GTPase mediated signal transduction Source: Reactome
    8. T cell differentiation Source: UniProtKB

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_11051. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho-related GTP-binding protein RhoH
    Alternative name(s):
    GTP-binding protein TTF
    Translocation three four protein
    Gene namesi
    Name:RHOH
    Synonyms:ARHH, TTF
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:686. RHOH.

    Subcellular locationi

    Cytoplasm 2 Publications. Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
    Note: Colocalizes together with ZAP70 in the immunological synapse.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytosol Source: Reactome
    3. immunological synapse Source: Ensembl
    4. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.

    Organism-specific databases

    Orphaneti324294. T-cell immunodeficiency with epidermodysplasia verruciformis.
    PharmGKBiPA24979.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Rho-related GTP-binding protein RhoHPRO_0000198867Add
    BLAST
    Propeptidei189 – 1913Removed in mature formBy similarityPRO_0000281218

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Cysteine methyl esterBy similarity
    Lipidationi188 – 1881S-geranylgeranyl cysteineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine by LCK. Phosphorylated by FYN. Phosphorylation enhances the interactions with ZAP70 and SYK and is critical for its function in thymocyte development By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    MaxQBiQ15669.
    PaxDbiQ15669.
    PRIDEiQ15669.

    Expressioni

    Tissue specificityi

    Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis.2 Publications

    Inductioni

    By CSF2/GM-CSF. Down-regulated by phorbol myristate acetate (PMA).2 Publications

    Gene expression databases

    ArrayExpressiQ15669.
    BgeeiQ15669.
    CleanExiHS_RHOH.
    GenevestigatoriQ15669.

    Organism-specific databases

    HPAiHPA030345.

    Interactioni

    Subunit structurei

    Interacts with ZAP70 (via SH2 domains) and the interaction is enhanced by its phosphorylation by LCK. Interacts with SYK and the interaction is enhanced by its phosphorylation by FYN By similarity. Interacts with GDI1 and GDI2. Interacts with PAK7/PAK5 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi106892. 6 interactions.
    MINTiMINT-1216771.
    STRINGi9606.ENSP00000371219.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15669.
    SMRiQ15669. Positions 4-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni73 – 8614Interaction with ZAP70By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi33 – 419Effector regionBy similarity

    Domaini

    The region involved in interaction with ZAP70 is a non-canonical immunoreceptor tyrosine-based activation motif (ITAM).By similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiQ15669.
    KOiK07873.
    OMAiASCINAI.
    OrthoDBiEOG7TJ3K0.
    PhylomeDBiQ15669.
    TreeFamiTF331219.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q15669-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSSIKCVLV GDSAVGKTSL LVRFTSETFP EAYKPTVYEN TGVDVFMDGI    50
    QISLGLWDTA GNDAFRSIRP LSYQQADVVL MCYSVANHNS FLNLKNKWIG 100
    EIRSNLPCTP VLVVATQTDQ REMGPHRASC VNAMEGKKLA QDVRAKGYLE 150
    CSALSNRGVQ QVFECAVRTA VNQARRRNRR RLFSINECKI F 191
    Length:191
    Mass (Da):21,331
    Last modified:November 1, 1997 - v1
    Checksum:iC5BF8929AD1E59D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35227 mRNA. Translation: CAA84538.1.
    AF498975 mRNA. Translation: AAM21122.1.
    BC014261 mRNA. Translation: AAH14261.1.
    CCDSiCCDS3458.1.
    PIRiI38367.
    RefSeqiNP_001265288.1. NM_001278359.1.
    NP_001265289.1. NM_001278360.1.
    NP_001265290.1. NM_001278361.1.
    NP_001265291.1. NM_001278362.1.
    NP_001265292.1. NM_001278363.1.
    NP_001265293.1. NM_001278364.1.
    NP_001265294.1. NM_001278365.1.
    NP_001265295.1. NM_001278366.1.
    NP_001265296.1. NM_001278367.1.
    NP_001265297.1. NM_001278368.1.
    NP_001265298.1. NM_001278369.1.
    NP_004301.1. NM_004310.4.
    XP_006714076.1. XM_006714013.1.
    UniGeneiHs.654594.
    Hs.738915.

    Genome annotation databases

    EnsembliENST00000381799; ENSP00000371219; ENSG00000168421.
    ENST00000505618; ENSP00000425010; ENSG00000168421.
    GeneIDi399.
    KEGGihsa:399.
    UCSCiuc003guz.2. human.

    Polymorphism databases

    DMDMi2500200.

    Keywords - Coding sequence diversityi

    Chromosomal rearrangement

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35227 mRNA. Translation: CAA84538.1 .
    AF498975 mRNA. Translation: AAM21122.1 .
    BC014261 mRNA. Translation: AAH14261.1 .
    CCDSi CCDS3458.1.
    PIRi I38367.
    RefSeqi NP_001265288.1. NM_001278359.1.
    NP_001265289.1. NM_001278360.1.
    NP_001265290.1. NM_001278361.1.
    NP_001265291.1. NM_001278362.1.
    NP_001265292.1. NM_001278363.1.
    NP_001265293.1. NM_001278364.1.
    NP_001265294.1. NM_001278365.1.
    NP_001265295.1. NM_001278366.1.
    NP_001265296.1. NM_001278367.1.
    NP_001265297.1. NM_001278368.1.
    NP_001265298.1. NM_001278369.1.
    NP_004301.1. NM_004310.4.
    XP_006714076.1. XM_006714013.1.
    UniGenei Hs.654594.
    Hs.738915.

    3D structure databases

    ProteinModelPortali Q15669.
    SMRi Q15669. Positions 4-171.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 106892. 6 interactions.
    MINTi MINT-1216771.
    STRINGi 9606.ENSP00000371219.

    Polymorphism databases

    DMDMi 2500200.

    Proteomic databases

    MaxQBi Q15669.
    PaxDbi Q15669.
    PRIDEi Q15669.

    Protocols and materials databases

    DNASUi 399.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000381799 ; ENSP00000371219 ; ENSG00000168421 .
    ENST00000505618 ; ENSP00000425010 ; ENSG00000168421 .
    GeneIDi 399.
    KEGGi hsa:399.
    UCSCi uc003guz.2. human.

    Organism-specific databases

    CTDi 399.
    GeneCardsi GC04P040192.
    HGNCi HGNC:686. RHOH.
    HPAi HPA030345.
    MIMi 602037. gene.
    neXtProti NX_Q15669.
    Orphaneti 324294. T-cell immunodeficiency with epidermodysplasia verruciformis.
    PharmGKBi PA24979.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi Q15669.
    KOi K07873.
    OMAi ASCINAI.
    OrthoDBi EOG7TJ3K0.
    PhylomeDBi Q15669.
    TreeFami TF331219.

    Enzyme and pathway databases

    Reactomei REACT_11051. Rho GTPase cycle.

    Miscellaneous databases

    GeneWikii RhoH.
    GenomeRNAii 399.
    NextBioi 1673.
    PROi Q15669.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15669.
    Bgeei Q15669.
    CleanExi HS_RHOH.
    Genevestigatori Q15669.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "TTF, a gene encoding a novel small G protein, fuses to the lymphoma-associated LAZ3 gene by t(3;4) chromosomal translocation."
      Dallery E., Galiegue-Zouitina S., Collyn-D'Hooghe M., Quief S., Denis C., Hildebrand M.-P., Lantoine D., Deweindt C., Tilly H., Bastard C., Kerckaert J.-P.
      Oncogene 10:2171-2178(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: B-cell.
    4. "The hematopoiesis-specific GTP-binding protein RhoH is GTPase deficient and modulates activities of other Rho GTPases by an inhibitory function."
      Li X., Bu X., Lu B., Avraham H., Flavell R.A., Lim B.
      Mol. Cell. Biol. 22:1158-1171(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GDI1 AND GDI2, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY.
    5. "Multiple Rho proteins regulate the subcellular targeting of PAK5."
      Wu X., Frost J.A.
      Biochem. Biophys. Res. Commun. 351:328-335(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PAK7.
    6. Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiRHOH_HUMAN
    AccessioniPrimary (citable) accession number: Q15669
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 142 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3