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Protein

NF-kappa-B inhibitor beta

Gene

NFKBIB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

GO - Molecular functioni

  • signal transducer activity Source: ProtInc
  • transcription coactivator activity Source: ProtInc

GO - Biological processi

  • cellular response to lipopolysaccharide Source: Ensembl
  • positive regulation of NF-kappaB transcription factor activity Source: Reactome
  • signal transduction Source: ProtInc
  • transcription, DNA-templated Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104825-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1810476. RIP-mediated NFkB activation via ZBP1.
R-HSA-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-HSA-5603027. IKBKG deficiency causes anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) (via TLR).
R-HSA-933542. TRAF6 mediated NF-kB activation.
SABIO-RKQ15653.
SignaLinkiQ15653.
SIGNORiQ15653.

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor beta
Short name:
NF-kappa-BIB
Alternative name(s):
I-kappa-B-beta
Short name:
IkB-B
Short name:
IkB-beta
Short name:
IkappaBbeta
Thyroid receptor-interacting protein 9
Short name:
TR-interacting protein 9
Short name:
TRIP-9
Gene namesi
Name:NFKBIB
Synonyms:IKBB, TRIP9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:7798. NFKBIB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19S → A: No degradation; when associated with A-23. 1 Publication1
Mutagenesisi23S → A: No degradation; when associated with A-19. 1 Publication1

Organism-specific databases

DisGeNETi4793.
OpenTargetsiENSG00000104825.
ENSG00000282905.
PharmGKBiPA31602.

Polymorphism and mutation databases

BioMutaiNFKBIB.
DMDMi57015399.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670041 – 356NF-kappa-B inhibitor betaAdd BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Phosphoserine; by RPS6KA11 Publication1
Modified residuei23Phosphoserine; by RPS6KA11 Publication1
Modified residuei183PhosphoserineCombined sources1
Modified residuei313Phosphoserine; by CK21 Publication1
Modified residuei315Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ15653.
MaxQBiQ15653.
PaxDbiQ15653.
PeptideAtlasiQ15653.
PRIDEiQ15653.

PTM databases

iPTMnetiQ15653.
PhosphoSitePlusiQ15653.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

BgeeiENSG00000104825.
CleanExiHS_NFKBIB.
ExpressionAtlasiQ15653. baseline and differential.
GenevisibleiQ15653. HS.

Organism-specific databases

HPAiCAB010447.
HPA063734.

Interactioni

Subunit structurei

Interacts with THRB (via ligand-binding domain) (PubMed:7776974). Interacts with RELA and REL (By similarity). Interacts with COMMD1 (PubMed:16573520). Interacts with inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2 (PubMed:10657303, PubMed:12672800, PubMed:15024091). Interacts with DDRGK1; positively regulates NFKBIB phosphorylation and degradation (PubMed:23675531).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P143402EBI-352889,EBI-465733From a different organism.
FBXW11Q9UKB12EBI-352889,EBI-355189
RELAQ042066EBI-352889,EBI-73886
VPS52Q8N1B43EBI-352889,EBI-2799833

Protein-protein interaction databases

BioGridi110860. 35 interactors.
DIPiDIP-27532N.
IntActiQ15653. 32 interactors.
MINTiMINT-1131598.
STRINGi9606.ENSP00000312988.

Structurei

3D structure databases

ProteinModelPortaliQ15653.
SMRiQ15653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 86ANK 1Add BLAST30
Repeati93 – 122ANK 2Add BLAST30
Repeati126 – 155ANK 3Add BLAST30
Repeati206 – 235ANK 4Add BLAST30
Repeati240 – 269ANK 5Add BLAST30
Repeati273 – 302ANK 6Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi186 – 195Asp/Glu-rich (acidic)10

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOVERGENiHBG019039.
InParanoidiQ15653.
KOiK02581.
OMAiDEWCDSG.
OrthoDBiEOG091G092R.
PhylomeDBiQ15653.
TreeFamiTF320166.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15653-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGVACLGKA ADADEWCDSG LGSLGPDAAA PGGPGLGAEL GPGLSWAPLV
60 70 80 90 100
FGYVTEDGDT ALHLAVIHQH EPFLDFLLGF SAGTEYMDLQ NDLGQTALHL
110 120 130 140 150
AAILGETSTV EKLYAAGAGL CVAERRGHTA LHLACRVGAH ACARALLQPR
160 170 180 190 200
PRRPREAPDT YLAQGPDRTP DTNHTPVALY PDSDLEKEEE ESEEDWKLQL
210 220 230 240 250
EAENYEGHTP LHVAVIHKDV EMVRLLRDAG ADLDKPEPTC GRSPLHLAVE
260 270 280 290 300
AQAADVLELL LRAGANPAAR MYGGRTPLGS AMLRPNPILA RLLRAHGAPE
310 320 330 340 350
PEGEDEKSGP CSSSSDSDSG DEGDEYDDIV VHSSRSQTRL PPTPASKPLP

DDPRPV
Length:356
Mass (Da):37,771
Last modified:January 4, 2005 - v2
Checksum:iE84575971B6F81CC
GO
Isoform 2 (identifier: Q15653-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-338: DEYDDIVVHSSRSQT → VSQEERQGSPAGGSG
     339-356: Missing.

Show »
Length:338
Mass (Da):35,530
Checksum:iF024666B851DF976
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19S → T in AAC41742 (PubMed:7776974).Curated1
Sequence conflicti319S → G in AAC41742 (PubMed:7776974).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020771339R → W.1 PublicationCorresponds to variant rs17886215dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012409324 – 338DEYDD…SRSQT → VSQEERQGSPAGGSG in isoform 2. 2 PublicationsAdd BLAST15
Alternative sequenceiVSP_012410339 – 356Missing in isoform 2. 2 PublicationsAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40407 Genomic DNA. Translation: AAC41742.1.
BT006743 mRNA. Translation: AAP35389.1.
AY736284 Genomic DNA. Translation: AAU10088.1.
AK290569 mRNA. Translation: BAF83258.1.
CH471126 Genomic DNA. Translation: EAW56843.1.
BC007197 mRNA. Translation: AAH07197.1.
BC015528 mRNA. Translation: AAH15528.1.
CCDSiCCDS12524.1. [Q15653-1]
RefSeqiNP_001230045.1. NM_001243116.1.
NP_002494.2. NM_002503.4. [Q15653-1]
UniGeneiHs.9731.

Genome annotation databases

EnsembliENST00000313582; ENSP00000312988; ENSG00000104825. [Q15653-1]
ENST00000572515; ENSP00000459728; ENSG00000104825. [Q15653-2]
ENST00000634647; ENSP00000489145; ENSG00000282905. [Q15653-2]
ENST00000635517; ENSP00000489138; ENSG00000282905. [Q15653-1]
GeneIDi4793.
KEGGihsa:4793.
UCSCiuc002ojw.4. human. [Q15653-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40407 Genomic DNA. Translation: AAC41742.1.
BT006743 mRNA. Translation: AAP35389.1.
AY736284 Genomic DNA. Translation: AAU10088.1.
AK290569 mRNA. Translation: BAF83258.1.
CH471126 Genomic DNA. Translation: EAW56843.1.
BC007197 mRNA. Translation: AAH07197.1.
BC015528 mRNA. Translation: AAH15528.1.
CCDSiCCDS12524.1. [Q15653-1]
RefSeqiNP_001230045.1. NM_001243116.1.
NP_002494.2. NM_002503.4. [Q15653-1]
UniGeneiHs.9731.

3D structure databases

ProteinModelPortaliQ15653.
SMRiQ15653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110860. 35 interactors.
DIPiDIP-27532N.
IntActiQ15653. 32 interactors.
MINTiMINT-1131598.
STRINGi9606.ENSP00000312988.

PTM databases

iPTMnetiQ15653.
PhosphoSitePlusiQ15653.

Polymorphism and mutation databases

BioMutaiNFKBIB.
DMDMi57015399.

Proteomic databases

EPDiQ15653.
MaxQBiQ15653.
PaxDbiQ15653.
PeptideAtlasiQ15653.
PRIDEiQ15653.

Protocols and materials databases

DNASUi4793.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000313582; ENSP00000312988; ENSG00000104825. [Q15653-1]
ENST00000572515; ENSP00000459728; ENSG00000104825. [Q15653-2]
ENST00000634647; ENSP00000489145; ENSG00000282905. [Q15653-2]
ENST00000635517; ENSP00000489138; ENSG00000282905. [Q15653-1]
GeneIDi4793.
KEGGihsa:4793.
UCSCiuc002ojw.4. human. [Q15653-1]

Organism-specific databases

CTDi4793.
DisGeNETi4793.
GeneCardsiNFKBIB.
HGNCiHGNC:7798. NFKBIB.
HPAiCAB010447.
HPA063734.
MIMi604495. gene.
neXtProtiNX_Q15653.
OpenTargetsiENSG00000104825.
ENSG00000282905.
PharmGKBiPA31602.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOVERGENiHBG019039.
InParanoidiQ15653.
KOiK02581.
OMAiDEWCDSG.
OrthoDBiEOG091G092R.
PhylomeDBiQ15653.
TreeFamiTF320166.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104825-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1810476. RIP-mediated NFkB activation via ZBP1.
R-HSA-445989. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-HSA-5603027. IKBKG deficiency causes anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) (via TLR).
R-HSA-933542. TRAF6 mediated NF-kB activation.
SABIO-RKQ15653.
SignaLinkiQ15653.
SIGNORiQ15653.

Miscellaneous databases

ChiTaRSiNFKBIB. human.
GeneWikiiNFKBIB.
GenomeRNAii4793.
PROiQ15653.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104825.
CleanExiHS_NFKBIB.
ExpressionAtlasiQ15653. baseline and differential.
GenevisibleiQ15653. HS.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIKBB_HUMAN
AccessioniPrimary (citable) accession number: Q15653
Secondary accession number(s): A8K3F4, Q96BJ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 4, 2005
Last modified: November 2, 2016
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.