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Q15645

- PCH2_HUMAN

UniProt

Q15645 - PCH2_HUMAN

Protein

Pachytene checkpoint protein 2 homolog

Gene

TRIP13

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (10 Jan 2006)
      Previous versions | rss
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    Functioni

    Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi179 – 1868ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. nucleoside-triphosphatase activity Source: InterPro
    3. protein binding Source: IntAct
    4. transcription cofactor activity Source: ProtInc

    GO - Biological processi

    1. double-strand break repair Source: UniProtKB
    2. female meiosis I Source: Ensembl
    3. male meiosis I Source: Ensembl
    4. oocyte maturation Source: Ensembl
    5. oogenesis Source: UniProtKB
    6. reciprocal meiotic recombination Source: UniProtKB
    7. regulation of RNA biosynthetic process Source: GOC
    8. spermatid development Source: Ensembl
    9. spermatogenesis Source: UniProtKB
    10. synaptonemal complex assembly Source: UniProtKB
    11. transcription from RNA polymerase II promoter Source: ProtInc

    Keywords - Biological processi

    Differentiation, Meiosis, Oogenesis, Spermatogenesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pachytene checkpoint protein 2 homolog
    Alternative name(s):
    Human papillomavirus type 16 E1 protein-binding protein
    Short name:
    16E1-BP
    Short name:
    HPV16 E1 protein-binding protein
    Thyroid hormone receptor interactor 13
    Thyroid receptor-interacting protein 13
    Short name:
    TR-interacting protein 13
    Short name:
    TRIP-13
    Gene namesi
    Name:TRIP13
    Synonyms:PCH2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:12307. TRIP13.

    Subcellular locationi

    GO - Cellular componenti

    1. male germ cell nucleus Source: Ensembl
    2. nucleus Source: ProtInc

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36986.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Pachytene checkpoint protein 2 homologPRO_0000084782Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ15645.
    PaxDbiQ15645.
    PeptideAtlasiQ15645.
    PRIDEiQ15645.

    PTM databases

    PhosphoSiteiQ15645.

    Expressioni

    Gene expression databases

    BgeeiQ15645.
    CleanExiHS_TRIP13.
    GenevestigatoriQ15645.

    Organism-specific databases

    HPAiHPA005727.
    HPA053093.

    Interactioni

    Subunit structurei

    Specifically interacts with the ligand binding domain of the thyroid receptor (TR). This interaction does not require the presence of thyroid hormone for its interaction. Interacts with HPV16 E1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARL11Q969Q43EBI-358993,EBI-751892
    C11orf54Q9H0W93EBI-358993,EBI-740204
    COMTQ9BWC73EBI-358993,EBI-743305
    DDAH2O958653EBI-358993,EBI-749139
    GLYCTKQ8IVS83EBI-358993,EBI-748515
    HDHD3Q9BSH53EBI-358993,EBI-745201
    LASP1Q148474EBI-358993,EBI-742828
    LOXL4Q96JB63EBI-358993,EBI-749562
    MAD2L1BPQ150135EBI-358993,EBI-712181
    NIF3L1Q9GZT83EBI-358993,EBI-740897
    PARK2O602603EBI-358993,EBI-716346
    PPP2CAP677753EBI-358993,EBI-712311
    TLDC1Q6P9B63EBI-358993,EBI-746504
    WDYHV1Q96HA83EBI-358993,EBI-741158

    Protein-protein interaction databases

    BioGridi114730. 74 interactions.
    DIPiDIP-34493N.
    IntActiQ15645. 58 interactions.
    MINTiMINT-1146352.
    STRINGi9606.ENSP00000166345.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15645.
    SMRiQ15645. Positions 71-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family. PCH2 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0464.
    HOGENOMiHOG000234557.
    HOVERGENiHBG052830.
    InParanoidiQ15645.
    OMAiAASHWVL.
    OrthoDBiEOG71VST9.
    PhylomeDBiQ15645.
    TreeFamiTF313507.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR001270. ClpA/B.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    PRINTSiPR00300. CLPPROTEASEA.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q15645-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDEAVGDLKQ ALPCVAESPT VHVEVHQRGS STAKKEDINL SVRKLLNRHN    50
    IVFGDYTWTE FDEPFLTRNV QSVSIIDTEL KVKDSQPIDL SACTVALHIF 100
    QLNEDGPSSE NLEEETENII AANHWVLPAA EFHGLWDSLV YDVEVKSHLL 150
    DYVMTTLLFS DKNVNSNLIT WNRVVLLHGP PGTGKTSLCK ALAQKLTIRL 200
    SSRYRYGQLI EINSHSLFSK WFSESGKLVT KMFQKIQDLI DDKDALVFVL 250
    IDEVESLTAA RNACRAGTEP SDAIRVVNAV LTQIDQIKRH SNVVILTTSN 300
    ITEKIDVAFV DRADIKQYIG PPSAAAIFKI YLSCLEELMK CQIIYPRQQL 350
    LTLRELEMIG FIENNVSKLS LLLNDISRKS EGLSGRVLRK LPFLAHALYV 400
    QAPTVTIEGF LQALSLAVDK QFEERKKLAA YI 432
    Length:432
    Mass (Da):48,551
    Last modified:January 10, 2006 - v2
    Checksum:iDFB0B37462D1D581
    GO
    Isoform 2 (identifier: Q15645-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         171-406: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:196
    Mass (Da):22,084
    Checksum:i74AF9425508B518A
    GO

    Sequence cautioni

    The sequence AAC41732.1 differs from that shown. Reason: Frameshift at positions 1 and 5.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei171 – 406236Missing in isoform 2. 1 PublicationVSP_016957Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U96131 mRNA. Translation: AAB64095.1.
    CR456744 mRNA. Translation: CAG33025.1.
    AC122719 Genomic DNA. No translation available.
    CH471102 Genomic DNA. Translation: EAX08185.1.
    CH471102 Genomic DNA. Translation: EAX08186.1.
    BC000404 mRNA. Translation: AAH00404.1.
    BC019294 mRNA. Translation: AAH19294.1.
    L40384 mRNA. Translation: AAC41732.1. Frameshift.
    CCDSiCCDS3858.1. [Q15645-1]
    RefSeqiNP_004228.1. NM_004237.3. [Q15645-1]
    UniGeneiHs.436187.

    Genome annotation databases

    EnsembliENST00000166345; ENSP00000166345; ENSG00000071539. [Q15645-1]
    GeneIDi9319.
    KEGGihsa:9319.
    UCSCiuc003jbr.3. human. [Q15645-1]

    Polymorphism databases

    DMDMi85541056.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U96131 mRNA. Translation: AAB64095.1 .
    CR456744 mRNA. Translation: CAG33025.1 .
    AC122719 Genomic DNA. No translation available.
    CH471102 Genomic DNA. Translation: EAX08185.1 .
    CH471102 Genomic DNA. Translation: EAX08186.1 .
    BC000404 mRNA. Translation: AAH00404.1 .
    BC019294 mRNA. Translation: AAH19294.1 .
    L40384 mRNA. Translation: AAC41732.1 . Frameshift.
    CCDSi CCDS3858.1. [Q15645-1 ]
    RefSeqi NP_004228.1. NM_004237.3. [Q15645-1 ]
    UniGenei Hs.436187.

    3D structure databases

    ProteinModelPortali Q15645.
    SMRi Q15645. Positions 71-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114730. 74 interactions.
    DIPi DIP-34493N.
    IntActi Q15645. 58 interactions.
    MINTi MINT-1146352.
    STRINGi 9606.ENSP00000166345.

    PTM databases

    PhosphoSitei Q15645.

    Polymorphism databases

    DMDMi 85541056.

    Proteomic databases

    MaxQBi Q15645.
    PaxDbi Q15645.
    PeptideAtlasi Q15645.
    PRIDEi Q15645.

    Protocols and materials databases

    DNASUi 9319.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000166345 ; ENSP00000166345 ; ENSG00000071539 . [Q15645-1 ]
    GeneIDi 9319.
    KEGGi hsa:9319.
    UCSCi uc003jbr.3. human. [Q15645-1 ]

    Organism-specific databases

    CTDi 9319.
    GeneCardsi GC05P000892.
    HGNCi HGNC:12307. TRIP13.
    HPAi HPA005727.
    HPA053093.
    MIMi 604507. gene.
    neXtProti NX_Q15645.
    PharmGKBi PA36986.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0464.
    HOGENOMi HOG000234557.
    HOVERGENi HBG052830.
    InParanoidi Q15645.
    OMAi AASHWVL.
    OrthoDBi EOG71VST9.
    PhylomeDBi Q15645.
    TreeFami TF313507.

    Miscellaneous databases

    GeneWikii TRIP13.
    GenomeRNAii 9319.
    NextBioi 34909.
    PROi Q15645.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q15645.
    CleanExi HS_TRIP13.
    Genevestigatori Q15645.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR001270. ClpA/B.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    PRINTSi PR00300. CLPPROTEASEA.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two classes of human papillomavirus type 16 E1 mutants suggest pleiotropic conformational constraints affecting E1 multimerization, E2 interaction, and interaction with cellular proteins."
      Yasugi T., Vidal M., Sakai H., Howley P.M., Benson J.D.
      J. Virol. 71:5942-5951(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH HPV16 E1.
    2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    5. "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor."
      Lee J.W., Choi H.-S., Gyuris J., Brent R., Moore D.D.
      Mol. Endocrinol. 9:243-254(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-417 (ISOFORM 2), INTERACTION WITH THRA.
    6. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPCH2_HUMAN
    AccessioniPrimary (citable) accession number: Q15645
    Secondary accession number(s): C9K0T3, D3DTC0, O15324
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 10, 2006
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3