Reviewed, UniProtKB/Swiss-Prot Q15637 (SF01_HUMAN)

Names and origin

Protein names

Recommended name:
    Splicing factor 1
Alternative name(s):
    Zinc finger protein 162
    Transcription factor ZFM1
    Zinc finger gene in MEN1 locus
    Mammalian branch point-binding protein mBBP
      Short name=BBP

Gene names

Name:	SF1
Synonyms:	ZFM1, ZNF162

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Ref.1 Ref.7 Ref.9
Subunit structure	Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Ref.1 Ref.7 Ref.9 Ref.8
Subcellular location	Nucleus.
Tissue specificity	Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart. Ref.3
Post-translational modification	Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463. Ref.7 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Sequence similarities	Belongs to the BBP/SF1 family. Contains 1 CCHC-type zinc finger. Contains 1 KH domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Repressor
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nuclear mRNA 3'-splice site recognition Traceable author statement. Source: HGNC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribosome Ref.1 Non-traceable author statement. Source: UniProtKB spliceosome Inferred from direct assay. Source: HGNC
Molecular function	RNA binding Ref.1 Traceable author statement. Source: UniProtKB RNA polymerase II transcription factor activity Ref.9 Traceable author statement. Source: ProtInc transcription corepressor activity Ref.9 Traceable author statement. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
E2	P03120	2	EBI-744603,EBI-1779322	From a different organism.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q15637-1)

Also known as: SF1-HL1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15637-2)

Also known as: SF1-Bo; Bone;

The sequence of this isoform differs from the canonical sequence as follows:
     597-639: YAPPPPPPPP...PAPPPPPPQN → IPPRGGDGPS...WWTGWFGKAA

Isoform 3 (identifier: Q15637-3)

Also known as: ZFM1-A; ZFM1-ABCDEF;

The sequence of this isoform differs from the canonical sequence as follows:
     587-639: PPPPGSAGMM...PAPPPPPPQN → RSIECLLCLL...PSPRRRWPEP

Isoform 4 (identifier: Q15637-4)

Also known as: ZFM1-B; ZFM1-ABCDF;

The sequence of this isoform differs from the canonical sequence as follows:
     528-548: NTTTTTTSAGTGSIPPWQQQQ → RSLPAAAMARAMRVRTFRAHW
     549-639: Missing.

Isoform 5 (identifier: Q15637-5)

The sequence of this isoform differs from the canonical sequence as follows:
     10-10: L → LGKLGPPGLP...QQPGPAGGGG
     528-548: NTTTTTTSAGTGSIPPWQQQQ → RSLPAAAMARAMRVRTFRAHW

Note: No experimental confirmation available.

Isoform 6 (identifier: Q15637-6)

Also known as: ZFM1-D; B6;

The sequence of this isoform differs from the canonical sequence as follows:
     448-548: DQYLGSTPVG...GSIPPWQQQQ → GKSVPGKYACGLWGLSPASRKRYDAATTYGHDA
     555-639: PGAPQMQGNP...PAPPPPPPQN → QWAAPTPSLW...WWTGWFGKAA

Note: Phosphorylated on Ser-463.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.6

Chain

2 – 639

638

Splicing factor 1

PRO_0000050129

Regions

Domain

141 – 222

82

KH

Zinc finger

277 – 296

20

CCHC-type

Motif

15 – 19

5

Nuclear localization signal Potential

Compositional bias

324 – 637

314

Pro-rich

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.6

Modified residue

20

1

Phosphoserine; by PKG Ref.7 Ref.14 Ref.15 Ref.16

Modified residue

80

1

Phosphoserine Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16

Modified residue

82

1

Phosphoserine Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16

Modified residue

87

1

Phosphotyrosine Ref.14

Modified residue

89

1

Phosphoserine Ref.16

Natural variations

Alternative sequence

10

1

L → LGKLGPPGLPPLPGPKGGFE PGPPPAPGPGAGLLAPGPPP PPPVGSMGALTAAFPFAALP PPPPPPPPPPPQQPPPPPPP PSPGASYPPPQPPPPPPLYQ RVSPPQPPPPQPPRKDQQPG PAGGGG in isoform 5.

VSP_008833

Alternative sequence

448 – 548

101

DQYLG…WQQQQ → GKSVPGKYACGLWGLSPASR KRYDAATTYGHDA in isoform 6.

VSP_008834

Alternative sequence

528 – 548

21

NTTTT…WQQQQ → RSLPAAAMARAMRVRTFRAH W in isoform 4 and isoform 5.

VSP_008835

Alternative sequence

549 – 639

91

Missing in isoform 4.

VSP_008836

Alternative sequence

555 – 639

85

PGAPQ…PPPQN → QWAAPTPSLWSSSPMATTAA AASATPSAQQQYGFQYPLAM AAKIPPRGGDGPSHESEDFP RPLVTLPGRQPQQRPWWTGW FGKAA in isoform 6.

VSP_008837

Alternative sequence

587 – 639

53

PPPPG…PPPQN → RSIECLLCLLSLLTQLPLPL PKPGRQDPSPRRRWPEP in isoform 3.

VSP_008838

Alternative sequence

597 – 639

43

YAPPP…PPPQN → IPPRGGDGPSHESEDFPRPL VTLPGRQPQQRPWWTGWFGK AA in isoform 2.

VSP_008839

Natural variant

357

1

S → T Ref.1

VAR_017196

Experimental info

Mutagenesis

15 – 17

3

KKR → EED: Abolishes interaction with U2AF2.

Mutagenesis

16 – 18

3

KRK → EDE: Abolishes interaction with U2AF2.

Mutagenesis

20

1

S → A: Strongly decreases interaction with U2AF2 and spliceosome assembly. Ref.7

Mutagenesis

20

1

S → T: Decreases interaction with U2AF2. Ref.7

Mutagenesis

21

1

R → A: Decreases interaction with U2AF2 and spliceosome assembly. Ref.18

Mutagenesis

21

1

R → K: No effect. Ref.18

Mutagenesis

22

1

W → A: Abolishes interaction with U2AF2. Ref.18

Mutagenesis

22

1

W → F: No effect. Ref.18

Mutagenesis

151

1

N → A: Decreases RNA-binding. Ref.17

Mutagenesis

160

1

R → A: Strongly reduces RNA-binding. Ref.17

Mutagenesis

184

1

K → A: Abolishes RNA-binding. Ref.17

Mutagenesis

244

1

L → A: Decreases RNA-binding. Ref.17

Mutagenesis

247

1

L → A: Decreases RNA-binding. Ref.17

Mutagenesis

254

1

L → A: Slightly decreases RNA-binding. Ref.17

Mutagenesis

255

1

R → A: Slightly decreases RNA-binding. Ref.17

Sequence conflict

269

1

E → G in BAA05116. Ref.3

Sequence conflict

269

1

E → G in BAA05117. Ref.3

Sequence conflict

348

1

A → R in AAB03514. Ref.2

Sequence conflict

348

1

A → R in AAB04033. Ref.2

Sequence conflict

377

1

R → W in BAA05116. Ref.3

Sequence conflict

377

1

R → W in BAA05117. Ref.3

Sequence conflict

591

1

G → V in AAB04033. Ref.2

Sequence conflict

623

1

M → I in AAB04033. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

639

Helix Strand Turn

Details...

Beta strand

136 – 141

6

Turn

144 – 146

3

Helix

150 – 157

8

Beta strand

159 – 161

3

Helix

162 – 170

9

Beta strand

174 – 190

17

Beta strand

203 – 211

9

Helix

212 – 226

15

Turn

227 – 230

4

Helix

238 – 244

7

Helix

245 – 248

4

Turn

249 – 252

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SF1-HL1) [UniParc]. Last modified January 23, 2007. Version 4. Checksum: EEBC6A02B29DAE4D	FASTA	639	68,330
	10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA NNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 550 560 570 580 590 600 IPPWQQQQAA AAASPGAPQM QGNPTMVPLP PGVQPPLPPG APPPPPPPPP GSAGMMYAPP 610 620 630 PPPPPPMDPS NFVTMMGMGV AGMPPFGMPP APPPPPPQN
	Isoform 2 (SF1-Bo) (Bone). Checksum: 06044F670EDC10C9	FASTA	638	68,633
	10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA NNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 550 560 570 580 590 600 IPPWQQQQAA AAASPGAPQM QGNPTMVPLP PGVQPPLPPG APPPPPPPPP GSAGMMIPPR 610 620 630 GGDGPSHESE DFPRPLVTLP GRQPQQRPWW TGWFGKAA
	Isoform 3 (ZFM1-A) (ZFM1-ABCDEF). Checksum: C07C74BBDC4093B7	FASTA	623	67,277
	10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA NNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQNTT TTTTSAGTGS 550 560 570 580 590 600 IPPWQQQQAA AAASPGAPQM QGNPTMVPLP PGVQPPLPPG APPPPPRSIE CLLCLLSLLT 610 620 QLPLPLPKPG RQDPSPRRRW PEP
	Isoform 4 (ZFM1-B) (ZFM1-ABCDF). Checksum: DDB42B66ABA2AD18	FASTA	548	59,712
	10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA NNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMDQY LGSTPVGSGV YRLHQGKGMM PPPPMGMMPP 490 500 510 520 530 540 PPPPPSGQPP PPPSGPLPPW QQQQQQPPPP PPPSSSMASS TPLPWQQRSL PAAAMARAMR VRTFRAHW
	Isoform 5. Checksum: 379C21D0B513F706	FASTA	764	80,620
	10 20 30 40 50 60 MATGANATPL GKLGPPGLPP LPGPKGGFEP GPPPAPGPGA GLLAPGPPPP PPVGSMGALT 70 80 90 100 110 120 AAFPFAALPP PPPPPPPPPP QQPPPPPPPP SPGASYPPPQ PPPPPPLYQR VSPPQPPPPQ 130 140 150 160 170 180 PPRKDQQPGP AGGGGDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ 190 200 210 220 230 240 LQIEDLTRKL RTGDLGIPPN PEDRSPSPEP IYNSEGKRLN TREFRTRKKL EEERHNLITE 250 260 270 280 290 300 MVALNPDFKP PADYKPPATR VSDKVMIPQD EYPEINFVGL LIGPRGNTLK NIEKECNAKI 310 320 330 340 350 360 MIRGKGSVKE GKVGRKDGQM LPGEDEPLHA LVTANTMENV KKAVEQIRNI LKQGIETPED 370 380 390 400 410 420 QNDLRKMQLR ELARLNGTLR EDDNRILRPW QSSETRSITN TTVCTKCGGA GHIASDCKFQ 430 440 450 460 470 480 RPGDPQSAQD KARMDKEYLS LMAELGEAPV PASVGSTSGP ATTPLASAPR PAAPANNPPP 490 500 510 520 530 540 PSLMSTTQSR PPWMNSGPSE SRPYHGMHGG GPGGPGGGPH SFPHPLPSLT GGHGGHPMQH 550 560 570 580 590 600 NPNGPPPPWM QPPPPPMNQG PHPPGHHGPP PMDQYLGSTP VGSGVYRLHQ GKGMMPPPPM 610 620 630 640 650 660 GMMPPPPPPP SGQPPPPPSG PLPPWQQQQQ QPPPPPPPSS SMASSTPLPW QQRSLPAAAM 670 680 690 700 710 720 ARAMRVRTFR AHWAAAAASP GAPQMQGNPT MVPLPPGVQP PLPPGAPPPP PPPPPGSAGM 730 740 750 760 MYAPPPPPPP PMDPSNFVTM MGMGVAGMPP FGMPPAPPPP PPQN
	Isoform 6 (ZFM1-D) (B6). Checksum: 5DA04D16D93EBDD5	FASTA	571	61,889
	10 20 30 40 50 60 MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED 70 80 90 100 110 120 LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH NLITEMVALN 130 140 150 160 170 180 PDFKPPADYK PPATRVSDKV MIPQDEYPEI NFVGLLIGPR GNTLKNIEKE CNAKIMIRGK 190 200 210 220 230 240 GSVKEGKVGR KDGQMLPGED EPLHALVTAN TMENVKKAVE QIRNILKQGI ETPEDQNDLR 250 260 270 280 290 300 KMQLRELARL NGTLREDDNR ILRPWQSSET RSITNTTVCT KCGGAGHIAS DCKFQRPGDP 310 320 330 340 350 360 QSAQDKARMD KEYLSLMAEL GEAPVPASVG STSGPATTPL ASAPRPAAPA NNPPPPSLMS 370 380 390 400 410 420 TTQSRPPWMN SGPSESRPYH GMHGGGPGGP GGGPHSFPHP LPSLTGGHGG HPMQHNPNGP 430 440 450 460 470 480 PPPWMQPPPP PMNQGPHPPG HHGPPPMGKS VPGKYACGLW GLSPASRKRY DAATTYGHDA 490 500 510 520 530 540 AAAAASQWAA PTPSLWSSSP MATTAAAASA TPSAQQQYGF QYPLAMAAKI PPRGGDGPSH 550 560 570 ESEDFPRPLV TLPGRQPQQR PWWTGWFGKA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA." Arning S., Grueter P., Bilbe G., Kraemer A. RNA 2:794-810(1996) [PubMed: 8752089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 20-30 AND 136-150, VARIANT THR-357, FUNCTION, INTERACTION WITH U1 SNRNA, RNA-BINDING. Tissue: Bone and Cervix carcinoma.
[2]	"Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins." Caslini C., Spinelli O., Cazzaniga G., Golay J., De Gioia L., Pedretti A., Breviario F., Amaru R., Barbui T., Biondi A., Introna M., Rambaldi A. Genomics 42:268-277(1997) [PubMed: 9192847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 6). Tissue: Myeloid leukemia cell.
[3]	"Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1)." Toda T., Iida A., Miwa T., Nakamura Y., Imai T. Hum. Mol. Genet. 3:465-470(1994) [PubMed: 7912130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), TISSUE SPECIFICITY. Tissue: Brain cortex, Cerebellum and Fetal liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 4 AND 5). Tissue: Brain, Eye, Kidney, Muscle and Skin.
[5]	"Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene." Kraemer A., Quentin M., Mulhauser F. Gene 211:29-37(1998) [PubMed: 9573336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-295.
[6]	Bienvenut W.V., Kanor S., Tissot J.-D., Quadroni M. Submitted (MAY-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-15, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: T-cell.
[7]	"Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly." Wang X., Bruderer S., Rafi Z., Xue J., Milburn P.J., Kraemer A., Robinson P.J. EMBO J. 18:4549-4559(1999) [PubMed: 10449420] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-28; 94-103; 228-239 AND 298-308, FUNCTION, INTERACTION WITH U2AF2, MUTAGENESIS OF SER-20, PHOSPHORYLATION AT SER-20.
[8]	"Large-scale proteomic analysis of the human spliceosome." Rappsilber J., Ryder U., Lamond A.I., Mann M. Genome Res. 12:1231-1245(2002) [PubMed: 12176931] [Abstract] Cited for: PROTEIN SEQUENCE OF 110-135, MASS SPECTROMETRY, INTERACTION WITH THE SPLICEOSOME.
[9]	"The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription." Zhang D., Paley A.J., Childs G. J. Biol. Chem. 273:18086-18091(1998) [PubMed: 9660765] [Abstract] Cited for: FUNCTION, INTERACTION WITH EWSR1; FUS AND TAF15.
[10]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY. Tissue: Epithelium.
[13]	"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line." Giorgianni F., Zhao Y., Desiderio D.M., Beranova-Giorgianni S. Electrophoresis 28:2027-2034(2007) [PubMed: 17487921] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80 AND SER-82, MASS SPECTROMETRY.
[14]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20; SER-80; SER-82 AND TYR-87, MASS SPECTROMETRY.
[15]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463 (ISOFORM 6), MASS SPECTROMETRY.
[16]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-82 AND SER-89, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463 (ISOFORM 6), MASS SPECTROMETRY.
[17]	"Structural basis for recognition of the intron branch site RNA by splicing factor 1." Liu Z., Luyten I., Bottomley M.J., Messias A.C., Houngninou-Molango S., Sprangers R., Zanier K., Kraemer A., Sattler M. Science 294:1098-1102(2001) [PubMed: 11691992] [Abstract] Cited for: STRUCTURE BY NMR OF 133-255 IN COMPLEX WITH THE BRANCH SITE SEQUENCE 5'-UAUACUAACAA-3', MUTAGENESIS OF ASN-151; ARG-160; LYS-184; LEU-244; LEU-247; LEU-254 AND ARG-255.
[18]	"Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP." Selenko P., Gregorovic G., Sprangers R., Stier G., Rhani Z., Kraemer A., Sattler M. Mol. Cell 11:965-976(2003) [PubMed: 12718882] [Abstract] Cited for: STRUCTURE BY NMR OF 13-25 IN COMPLEX WITH U2AF2, MUTAGENESIS OF 16-LYS--ARG-18; 17-LYS-LYS-18; ARG-21 AND TRP-22.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y08765 mRNA. Translation: CAA70018.1.
Y08766 mRNA. Translation: CAA70019.1.
L49345 mRNA. Translation: AAB03514.1.
L49380 mRNA. Translation: AAB04033.1.
D26120 mRNA. Translation: BAA05116.1.
D26120 mRNA. Translation: BAA05117.1.
BC000773 mRNA. Translation: AAH00773.1. Different initiation.
BC008080 mRNA. Translation: AAH08080.1.
BC008724 mRNA. Translation: AAH08724.1.
BC011657 mRNA. No translation available.
BC020217 mRNA. Translation: AAH20217.1.
BC038446 mRNA. Translation: AAH38446.1.
AJ000051, AJ000052 Genomic DNA. Translation: CAA03883.1.

IPI

IPI00294627.
IPI00386114.
IPI00386117.
IPI00386118.
IPI00386119.
IPI00386120.

PIR

G02919.

RefSeq

NP_004621.2.
NP_973724.1.
NP_973727.1.

UniGene

Hs.502829
Hs.708209

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K1G	NMR	-	A	133-260	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15637. 7 interactions.

Proteomic databases

PRIDE

Q15637.

Genome annotation databases

Ensembl

ENSG00000168066. Homo sapiens. [Contig view]

GeneID

7536.

KEGG

hsa:7536.

UCSC

uc001oaz.1. human.
uc001oba.1. human.
uc001obb.1. human.
uc001obc.1. human.
uc001obd.1. human.

Organism-specific databases

GeneCards

GC11M064290.

HGNC

HGNC:12950. SF1.

MIM

601516. gene.

PharmGKB

PA26841.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

Q15637.

OMA

Q15637. GSAGMMY.

Gene expression databases

ArrayExpress

Q15637.

Bgee

Q15637.

CleanEx

HS_SF1.

GermOnline

ENSG00000168066. Homo sapiens.

Family and domain databases

InterPro

IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR001878. Znf_CCHC.
[Graphical view]

Pfam

PF00013. KH_1. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]

SMART

SM00322. KH. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]

PROSITE

PS50084. KH_TYPE_1. 1 hit.
PS50158. ZF_CCHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

29487.

SOURCE

Search...

Entry information

Entry name

SF01_HUMAN

Accession

Primary (citable) accession number: Q15637
Secondary accession number(s): Q14818 , Q14819, Q15913, Q8IY00, Q92744, Q92745, Q969H7, Q9BW01, Q9UEI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 96 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)