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Protein

Translocating chain-associated membrane protein 1

Gene

TRAM1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stimulatory or required for the translocation of secretory proteins across the ER membrane.

GO - Molecular functioni

  • receptor activity Source: ProtInc

GO - Biological processi

  • cotranslational protein targeting to membrane Source: ProtInc
  • viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000067167-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocating chain-associated membrane protein 1
Gene namesi
Name:TRAM1
Synonyms:TRAM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:20568. TRAM1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 29CytoplasmicSequence analysisAdd BLAST28
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 76LumenalSequence analysisAdd BLAST26
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Topological domaini98 – 121CytoplasmicSequence analysisAdd BLAST24
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 159LumenalSequence analysisAdd BLAST17
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 192CytoplasmicSequence analysisAdd BLAST12
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 217LumenalSequence analysis4
Transmembranei218 – 238HelicalSequence analysisAdd BLAST21
Topological domaini239 – 251CytoplasmicSequence analysisAdd BLAST13
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Topological domaini273 – 297LumenalSequence analysisAdd BLAST25
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 374CytoplasmicSequence analysisAdd BLAST56

GO - Cellular componenti

  • endoplasmic reticulum Source: ParkinsonsUK-UCL
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: ProtInc
  • rough endoplasmic reticulum Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi23471.
OpenTargetsiENSG00000067167.
PharmGKBiPA134955644.

Polymorphism and mutation databases

BioMutaiTRAM1.
DMDMi18202507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001855302 – 374Translocating chain-associated membrane protein 1Add BLAST373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...)Curated1
Modified residuei365PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ15629.
PaxDbiQ15629.
PeptideAtlasiQ15629.
PRIDEiQ15629.
TopDownProteomicsiQ15629-1. [Q15629-1]

PTM databases

iPTMnetiQ15629.
PhosphoSitePlusiQ15629.
SwissPalmiQ15629.

Expressioni

Gene expression databases

BgeeiENSG00000067167.
CleanExiHS_TRAM1.
ExpressionAtlasiQ15629. baseline and differential.
GenevisibleiQ15629. HS.

Organism-specific databases

HPAiHPA023929.

Interactioni

Subunit structurei

Interacts with human cytomegalovirus/HHV-5 protein US2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BCAP31P515725EBI-1788852,EBI-77683

Protein-protein interaction databases

BioGridi117032. 20 interactors.
DIPiDIP-45978N.
IntActiQ15629. 21 interactors.
MINTiMINT-4825850.
STRINGi9606.ENSP00000262213.

Structurei

3D structure databases

ProteinModelPortaliQ15629.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 326TLCPROSITE-ProRule annotationAdd BLAST210

Sequence similaritiesi

Belongs to the TRAM family.Curated
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1608. Eukaryota.
ENOG410XSB3. LUCA.
GeneTreeiENSGT00510000046470.
HOGENOMiHOG000007494.
HOVERGENiHBG054402.
InParanoidiQ15629.
KOiK14010.
OMAiLQLAYWF.
OrthoDBiEOG091G077N.
PhylomeDBiQ15629.
TreeFamiTF314319.

Family and domain databases

InterProiIPR006634. TLC-dom.
IPR013599. TRAM1.
IPR016447. Translocation_assoc_membrane.
[Graphical view]
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005449. Translocation_assoc_membrane. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15629-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIRKKSTKS PPVLSHEFVL QNHADIVSCV AMVFLLGLMF EITAKASIIF
60 70 80 90 100
VTLQYNVTLP ATEEQATESV SLYYYGIKDL ATVFFYMLVA IIIHAVIQEY
110 120 130 140 150
MLDKINRRMH FSKTKHSKFN ESGQLSAFYL FACVWGTFIL ISENYISDPT
160 170 180 190 200
ILWRAYPHNL MTFQMKFFYI SQLAYWLHAF PELYFQKTKK EDIPRQLVYI
210 220 230 240 250
GLYLFHIAGA YLLNLNHLGL VLLVLHYFVE FLFHISRLFY FSNEKYQKGF
260 270 280 290 300
SLWAVLFVLG RLLTLILSVL TVGFGLARAE NQKLDFSTGN FNVLAVRIAV
310 320 330 340 350
LASICVTQAF MMWKFINFQL RRWREHSAFQ APAVKKKPTV TKGRSSKKGT
360 370
ENGVNGTLTS NVADSPRNKK EKSS
Length:374
Mass (Da):43,072
Last modified:January 23, 2007 - v3
Checksum:i9232ADA33DE20076
GO
Isoform 2 (identifier: Q15629-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.
Show »
Length:343
Mass (Da):39,683
Checksum:i14266BD8762EF466
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0562131 – 31Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63679 mRNA. Translation: CAA45218.1.
AK303411 mRNA. Translation: BAG64464.1.
AC022731 Genomic DNA. No translation available.
AC120194 Genomic DNA. No translation available.
BC000687 mRNA. Translation: AAH00687.1.
BC037738 mRNA. Translation: AAH37738.1.
CCDSiCCDS6207.1. [Q15629-1]
PIRiS30034.
RefSeqiNP_001304733.1. NM_001317804.1. [Q15629-2]
NP_001304734.1. NM_001317805.1.
NP_055109.1. NM_014294.5. [Q15629-1]
UniGeneiHs.491988.

Genome annotation databases

EnsembliENST00000262213; ENSP00000262213; ENSG00000067167. [Q15629-1]
GeneIDi23471.
KEGGihsa:23471.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63679 mRNA. Translation: CAA45218.1.
AK303411 mRNA. Translation: BAG64464.1.
AC022731 Genomic DNA. No translation available.
AC120194 Genomic DNA. No translation available.
BC000687 mRNA. Translation: AAH00687.1.
BC037738 mRNA. Translation: AAH37738.1.
CCDSiCCDS6207.1. [Q15629-1]
PIRiS30034.
RefSeqiNP_001304733.1. NM_001317804.1. [Q15629-2]
NP_001304734.1. NM_001317805.1.
NP_055109.1. NM_014294.5. [Q15629-1]
UniGeneiHs.491988.

3D structure databases

ProteinModelPortaliQ15629.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117032. 20 interactors.
DIPiDIP-45978N.
IntActiQ15629. 21 interactors.
MINTiMINT-4825850.
STRINGi9606.ENSP00000262213.

PTM databases

iPTMnetiQ15629.
PhosphoSitePlusiQ15629.
SwissPalmiQ15629.

Polymorphism and mutation databases

BioMutaiTRAM1.
DMDMi18202507.

Proteomic databases

EPDiQ15629.
PaxDbiQ15629.
PeptideAtlasiQ15629.
PRIDEiQ15629.
TopDownProteomicsiQ15629-1. [Q15629-1]

Protocols and materials databases

DNASUi23471.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262213; ENSP00000262213; ENSG00000067167. [Q15629-1]
GeneIDi23471.
KEGGihsa:23471.

Organism-specific databases

CTDi23471.
DisGeNETi23471.
GeneCardsiTRAM1.
HGNCiHGNC:20568. TRAM1.
HPAiHPA023929.
MIMi605190. gene.
neXtProtiNX_Q15629.
OpenTargetsiENSG00000067167.
PharmGKBiPA134955644.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1608. Eukaryota.
ENOG410XSB3. LUCA.
GeneTreeiENSGT00510000046470.
HOGENOMiHOG000007494.
HOVERGENiHBG054402.
InParanoidiQ15629.
KOiK14010.
OMAiLQLAYWF.
OrthoDBiEOG091G077N.
PhylomeDBiQ15629.
TreeFamiTF314319.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000067167-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

ChiTaRSiTRAM1. human.
GenomeRNAii23471.
PROiQ15629.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000067167.
CleanExiHS_TRAM1.
ExpressionAtlasiQ15629. baseline and differential.
GenevisibleiQ15629. HS.

Family and domain databases

InterProiIPR006634. TLC-dom.
IPR013599. TRAM1.
IPR016447. Translocation_assoc_membrane.
[Graphical view]
PfamiPF08390. TRAM1. 1 hit.
PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005449. Translocation_assoc_membrane. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAM1_HUMAN
AccessioniPrimary (citable) accession number: Q15629
Secondary accession number(s): B4E0K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.