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Protein

Dixin

Gene

DIXDC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.2 Publications

GO - Molecular functioni

  • gamma-tubulin binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dixin
Alternative name(s):
Coiled-coil protein DIX1
Short name:
Coiled-coil-DIX1
DIX domain-containing protein 1
Gene namesi
Name:DIXDC1
Synonyms:CCD1, KIAA1735
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:23695. DIXDC1.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB
  • focal adhesion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi648D → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1
Mutagenesisi651F → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1
Mutagenesisi655K → A: Loss of interaction with DVL2. Abolishes activation of Wnt signaling. 1 Publication1

Organism-specific databases

DisGeNETi85458.
OpenTargetsiENSG00000150764.
PharmGKBiPA134988674.

Polymorphism and mutation databases

DMDMi147641721.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872231 – 683DixinAdd BLAST683
Isoform 2 (identifier: Q155Q3-2)
Initiator methionineiRemoved

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186PhosphoserineBy similarity1
Modified residuei231PhosphoserineBy similarity1
Modified residuei590PhosphoserineCombined sources1
Isoform 2 (identifier: Q155Q3-2)
Lipidationi2N-myristoyl glycine1

Post-translational modificationi

Phosphorylated on tyrosine and serine residues.1 Publication
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ155Q3.
MaxQBiQ155Q3.
PaxDbiQ155Q3.
PeptideAtlasiQ155Q3.
PRIDEiQ155Q3.

PTM databases

iPTMnetiQ155Q3.
PhosphoSitePlusiQ155Q3.

Expressioni

Tissue specificityi

Ubiquitously expressed with higher expression in cardiac and skeletal muscles.1 Publication

Gene expression databases

BgeeiENSG00000150764.
CleanExiHS_DIXDC1.
ExpressionAtlasiQ155Q3. baseline and differential.
GenevisibleiQ155Q3. HS.

Organism-specific databases

HPAiHPA039424.
HPA039658.

Interactioni

Subunit structurei

Isoform 1 but not isoform 2 binds filamentous actin. Interacts with the complex composed of DVL2 and Rac. Interacts with AXIN1; competes with MAP3K1. Interacts with MAP3K4 preventing MAP3K4 interaction with AXIN1. Directly interacts (via DIX domain) with DVL2 (via DIX domain). Interacts with gamma-tubulin.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DVL2O146412EBI-1104700,EBI-740850
Dvl2Q608384EBI-1104700,EBI-641940From a different organism.

GO - Molecular functioni

  • gamma-tubulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124542. 19 interactors.
IntActiQ155Q3. 20 interactors.
MINTiMINT-8080298.
STRINGi9606.ENSP00000394352.

Structurei

Secondary structure

1683
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi601 – 606Combined sources6
Beta strandi614 – 620Combined sources7
Helixi627 – 634Combined sources8
Beta strandi640 – 648Combined sources9
Turni649 – 651Combined sources3
Beta strandi652 – 658Combined sources7
Beta strandi671 – 678Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PZ7X-ray2.44A597-683[»]
ProteinModelPortaliQ155Q3.
SMRiQ155Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 127CHPROSITE-ProRule annotationAdd BLAST108
Domaini600 – 680DIXPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni127 – 300Actin-bindingAdd BLAST174

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili279 – 452Sequence analysisAdd BLAST174

Domaini

The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.1 Publication
The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.1 Publication

Sequence similaritiesi

Belongs to the DIXDC1 family.Curated
Contains 1 CH (calponin-homology) domain.PROSITE-ProRule annotation
Contains 1 DIX domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFNC. Eukaryota.
ENOG410XPPR. LUCA.
GeneTreeiENSGT00390000013552.
HOVERGENiHBG107812.
InParanoidiQ155Q3.
OMAiETSWEEQ.
OrthoDBiEOG091G03RC.
PhylomeDBiQ155Q3.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR001158. DIX.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00778. DIX. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00021. DAX. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS50841. DIX. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q155Q3-1) [UniParc]FASTAAdd to basket
Also known as: l-DIXDC1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLACLTRGNL LDVLQEGFNE QQLQAYVAWV NAQLKKRPAV KPVQDLRQDL
60 70 80 90 100
RDGVILAYLI EIVAGEKLSG VQLSPGNQQE MKNNVEKVLQ FVASKKIRMH
110 120 130 140 150
QTSAKDIVDG NLKSIMRLVL ALAAHFKPGS SRTVNQGRDS RAPLQSHRPH
160 170 180 190 200
CATAVAQGAA AALADVCHDM SRSGRDVFRY RQRNSSMDEE IENPYWSVRA
210 220 230 240 250
LVQQYEGQQR SPSESSCSSL TSPSPIHSAK SESIITQSEE KADFVIIPAE
260 270 280 290 300
GIENRTEGTD SPLSRDWRPG SPGTYLETSW EEQLLEQQEY LEKEMEEAKK
310 320 330 340 350
MISGLQALLL NGSLPEDEQE RPLALCEPGV NPEEQLIIIQ SRLDQSMEEN
360 370 380 390 400
QDLKKELLKC KQEARNLQGI KDALQQRLTQ QDTSVLQLKQ ELLRANMDKD
410 420 430 440 450
ELHNQNVDLQ RKLDERNRLL GEYKKELGQK DRLLQQHQAK LEEALRKLSD
460 470 480 490 500
VSYHQVDLER ELEHKDVLLA HCMKREADEA TNYNSHNSQS NGFLLPTAGK
510 520 530 540 550
GATSVSNRGT SDLQLVRDAL RSLRNSFSGH DPQHHTIDSL EQGISSLMER
560 570 580 590 600
LHVMETQKKQ ERKVRVKSPR TQVGSEYRES WPPNSKLPHS QSSPTVSSTC
610 620 630 640 650
TKVLYFTDRS LTPFMVNIPK RLEEVTLKDF KAAIDREGNH RYHFKALDPE
660 670 680
FGTVKEEIFH DDDAIPGWEG KIVAWVEEDH GEN
Note: Major isoform. Ubiquitously expressed.
Length:683
Mass (Da):77,478
Last modified:May 15, 2007 - v2
Checksum:iE643D1A69B00238C
GO
Isoform 2 (identifier: Q155Q3-2) [UniParc]FASTAAdd to basket
Also known as: s-DIXDC1

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.
     212-219: PSESSCSS → MGGTQVKC

Note: Major isoform. Preferentially expressed in cardiac and skeletal muscles.
Show »
Length:472
Mass (Da):53,942
Checksum:i70C4312A59CAAF47
GO
Isoform 3 (identifier: Q155Q3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-219: S → R
     220-683: Missing.

Note: No experimental confirmation available.
Show »
Length:219
Mass (Da):24,428
Checksum:iF27E0386517E1EAB
GO
Isoform 4 (identifier: Q155Q3-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MLACLTRGNLLDVLQEGFNE → MGTQVVMRFNNSLLPTEPS
     219-219: S → R
     220-683: Missing.

Note: No experimental confirmation available.
Show »
Length:218
Mass (Da):24,313
Checksum:i7EC65502524E280C
GO

Sequence cautioni

The sequence AAH35509 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH64479 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAB21826 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB71039 differs from that shown. Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140S → P in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti144L → R in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti168H → Q in ABG25914 (PubMed:16814745).Curated1
Sequence conflicti378L → S in BAB71039 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032294300K → R.Corresponds to variant rs34575249dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0253781 – 211Missing in isoform 2. 2 PublicationsAdd BLAST211
Alternative sequenceiVSP_0545651 – 20MLACL…EGFNE → MGTQVVMRFNNSLLPTEPS in isoform 4. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_025379212 – 219PSESSCSS → MGGTQVKC in isoform 2. 2 Publications8
Alternative sequenceiVSP_025380219S → R in isoform 3 and isoform 4. 1 Publication1
Alternative sequenceiVSP_025381220 – 683Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST464

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ642016 mRNA. Translation: ABG25914.1.
AB051522 mRNA. Translation: BAB21826.1. Different initiation.
AP000907 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67171.1.
BC033034 mRNA. Translation: AAH33034.1.
BC035509 mRNA. Translation: AAH35509.1. Sequence problems.
BC041626 mRNA. Translation: AAH41626.2.
BC048294 mRNA. Translation: AAH48294.1.
BC064479 mRNA. Translation: AAH64479.1. Sequence problems.
BC128600 mRNA. Translation: AAI28601.1.
AK055899 mRNA. Translation: BAB71039.1. Sequence problems.
CCDSiCCDS60957.1. [Q155Q3-5]
CCDS73381.1. [Q155Q3-1]
CCDS73382.1. [Q155Q3-2]
RefSeqiNP_001033043.1. NM_001037954.3. [Q155Q3-1]
NP_001265471.1. NM_001278542.1. [Q155Q3-5]
NP_219493.1. NM_033425.4. [Q155Q3-2]
UniGeneiHs.655626.

Genome annotation databases

EnsembliENST00000440460; ENSP00000394352; ENSG00000150764. [Q155Q3-1]
ENST00000529225; ENSP00000434130; ENSG00000150764. [Q155Q3-5]
ENST00000615255; ENSP00000480808; ENSG00000150764. [Q155Q3-2]
GeneIDi85458.
KEGGihsa:85458.
UCSCiuc001pmj.4. human. [Q155Q3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ642016 mRNA. Translation: ABG25914.1.
AB051522 mRNA. Translation: BAB21826.1. Different initiation.
AP000907 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67171.1.
BC033034 mRNA. Translation: AAH33034.1.
BC035509 mRNA. Translation: AAH35509.1. Sequence problems.
BC041626 mRNA. Translation: AAH41626.2.
BC048294 mRNA. Translation: AAH48294.1.
BC064479 mRNA. Translation: AAH64479.1. Sequence problems.
BC128600 mRNA. Translation: AAI28601.1.
AK055899 mRNA. Translation: BAB71039.1. Sequence problems.
CCDSiCCDS60957.1. [Q155Q3-5]
CCDS73381.1. [Q155Q3-1]
CCDS73382.1. [Q155Q3-2]
RefSeqiNP_001033043.1. NM_001037954.3. [Q155Q3-1]
NP_001265471.1. NM_001278542.1. [Q155Q3-5]
NP_219493.1. NM_033425.4. [Q155Q3-2]
UniGeneiHs.655626.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PZ7X-ray2.44A597-683[»]
ProteinModelPortaliQ155Q3.
SMRiQ155Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124542. 19 interactors.
IntActiQ155Q3. 20 interactors.
MINTiMINT-8080298.
STRINGi9606.ENSP00000394352.

PTM databases

iPTMnetiQ155Q3.
PhosphoSitePlusiQ155Q3.

Polymorphism and mutation databases

DMDMi147641721.

Proteomic databases

EPDiQ155Q3.
MaxQBiQ155Q3.
PaxDbiQ155Q3.
PeptideAtlasiQ155Q3.
PRIDEiQ155Q3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000440460; ENSP00000394352; ENSG00000150764. [Q155Q3-1]
ENST00000529225; ENSP00000434130; ENSG00000150764. [Q155Q3-5]
ENST00000615255; ENSP00000480808; ENSG00000150764. [Q155Q3-2]
GeneIDi85458.
KEGGihsa:85458.
UCSCiuc001pmj.4. human. [Q155Q3-1]

Organism-specific databases

CTDi85458.
DisGeNETi85458.
GeneCardsiDIXDC1.
HGNCiHGNC:23695. DIXDC1.
HPAiHPA039424.
HPA039658.
MIMi610493. gene.
neXtProtiNX_Q155Q3.
OpenTargetsiENSG00000150764.
PharmGKBiPA134988674.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFNC. Eukaryota.
ENOG410XPPR. LUCA.
GeneTreeiENSGT00390000013552.
HOVERGENiHBG107812.
InParanoidiQ155Q3.
OMAiETSWEEQ.
OrthoDBiEOG091G03RC.
PhylomeDBiQ155Q3.

Miscellaneous databases

ChiTaRSiDIXDC1. human.
GeneWikiiDIXDC1.
GenomeRNAii85458.
PROiQ155Q3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000150764.
CleanExiHS_DIXDC1.
ExpressionAtlasiQ155Q3. baseline and differential.
GenevisibleiQ155Q3. HS.

Family and domain databases

CDDicd00014. CH. 1 hit.
Gene3Di1.10.418.10. 1 hit.
InterProiIPR001715. CH-domain.
IPR001158. DIX.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00307. CH. 1 hit.
PF00778. DIX. 1 hit.
[Graphical view]
SMARTiSM00033. CH. 1 hit.
SM00021. DAX. 1 hit.
[Graphical view]
SUPFAMiSSF47576. SSF47576. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50021. CH. 1 hit.
PS50841. DIX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDIXC1_HUMAN
AccessioniPrimary (citable) accession number: Q155Q3
Secondary accession number(s): A1A5D8
, E9PRV4, Q6P2J8, Q6PIK4, Q86SR7, Q8IVY4, Q96N69, Q9C0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: November 30, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.