Q15573 (TAF1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA box-binding protein-associated factor RNA polymerase I subunit A

Alternative name(s):
RNA polymerase I-specific TBP-associated factor 48 kDa
Short name=TAFI48
TATA box-binding protein-associated factor 1A
Short name=TBP-associated factor 1A
Transcription factor SL1
Transcription initiation factor SL1/TIF-IB subunit A

Gene names

Name:

TAF1A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Ref.1 Ref.7
Subunit structure	Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1B. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with UBFT. Ref.1 Ref.6
Subcellular location	Nucleus.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW termination of RNA polymerase I transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase I promoter Traceable author statement. Source: Reactome transcription from RNA polymerase II promoter Traceable author statement. Source: ProtInc transcription initiation from RNA polymerase I promoter Traceable author statement. Source: Reactome
Cellular component	RNA polymerase I transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15573-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15573-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	TATA box-binding protein-associated factor RNA polymerase I subunit A		PRO_0000227987
Natural variations
	Alternative sequence	1 – 114	114	Missing in isoform 2.		VSP_017635
	Natural variant	98	1	I → M. Corresponds to variant rs17163271 [ dbSNP | Ensembl ].		VAR_052253

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 130330D78493C4D8	FASTA	450	52,676
	10 20 30 40 50 60 MSDFSEELKG PVTDDEEVET SVLSGAGMHF PWLQTYVETV AIGGKRRKDF AQTTSACLSF 70 80 90 100 110 120 IQEALLKHQW QQAAEYMYSY FQTLEDSDSY KRQAAPEIIW KLGSEILFYH PKSNMESFNT 130 140 150 160 170 180 FANRMKNIGV MNYLKISLQH ALYLLHHGML KDAKRNLSEA ETWRHGENTS SREILINLIQ 190 200 210 220 230 240 AYKGLLQYYT WSEKKMELSK LDKDDYAYNA VAQDVFNHSW KTSANISALI KIPGVWDPFV 250 260 270 280 290 300 KSYVEMLEFY GDRDGAQEVL TNYAYDEKFP SNPNAHIYLY NFLKRQKAPR SKLISVLKIL 310 320 330 340 350 360 YQIVPSHKLM LEFHTLLRKS EKEEHRKLGL EVLFGVLDFA GCTKNITAWK YLAKYLKNIL 370 380 390 400 410 420 MGNHLAWVQE EWNSRKNWWP GFHFSYFWAK SDWKEDTALA CEKAFVAGLL LGKGCRYFRY 430 440 450 ILKQDHQILG KKIKRMKRSV KKYSIVNPRL
	Isoform 2 [UniParc]. Checksum: 16966A610BFC1078	FASTA	336	39,615
	10 20 30 40 50 60 MESFNTFANR MKNIGVMNYL KISLQHALYL LHHGMLKDAK RNLSEAETWR HGENTSSREI 70 80 90 100 110 120 LINLIQAYKG LLQYYTWSEK KMELSKLDKD DYAYNAVAQD VFNHSWKTSA NISALIKIPG 130 140 150 160 170 180 VWDPFVKSYV EMLEFYGDRD GAQEVLTNYA YDEKFPSNPN AHIYLYNFLK RQKAPRSKLI 190 200 210 220 230 240 SVLKILYQIV PSHKLMLEFH TLLRKSEKEE HRKLGLEVLF GVLDFAGCTK NITAWKYLAK 250 260 270 280 290 300 YLKNILMGNH LAWVQEEWNS RKNWWPGFHF SYFWAKSDWK EDTALACEKA FVAGLLLGKG 310 320 330 CRYFRYILKQ DHQILGKKIK RMKRSVKKYS IVNPRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits." Comai L., Zomerdijk J.C.B.M., Beckmann H., Zhou S., Admon A., Tjian R. Science 266:1966-1972(1994) [PubMed: 7801123] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 68-84; 113-123; 155-169; 183-193; 205-219; 221-230; 404-413 AND 442-449, FUNCTION, INTERACTION WITH TBP; TAF1B AND TAF1C.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Embryo and Kidney.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin.
[6]	"Coactivator and promoter-selective properties of RNA polymerase I TAFs." Beckmann H., Chen J.L., O'Brien T., Tjian R. Science 270:1506-1509(1995) [PubMed: 7491500] [Abstract] Cited for: INTERACTION WITH UBTF.
[7]	"TBP-TAF complex SL1 directs RNA polymerase I pre-initiation complex formation and stabilizes upstream binding factor at the rDNA promoter." Friedrich J.K., Panov K.I., Cabart P., Russell J., Zomerdijk J.C.B.M. J. Biol. Chem. 280:29551-29558(2005) [PubMed: 15970593] [Abstract] Cited for: FUNCTION OF THE SL1/TIF-IB COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39060 mRNA. Translation: AAA62862.1. AK001054 mRNA. Translation: BAA91482.1. AK315740 mRNA. Translation: BAG38095.1. AL592148 Genomic DNA. Translation: CAI40466.1. AL592148 Genomic DNA. Translation: CAI40467.1. CH471100 Genomic DNA. Translation: EAW93271.1. CH471100 Genomic DNA. Translation: EAW93272.1. BC013808 mRNA. Translation: AAH13808.1.
IPI	IPI00024263. IPI00157541.
PIR	I61580.
RefSeq	NP_001188465.1. NM_001201536.1. NP_005672.1. NM_005681.3. NP_647603.1. NM_139352.2.
UniGene	Hs.153088.
3D structure databases
ProteinModelPortal	Q15573.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15573. 1 interaction.
MINT	MINT-4329083.
STRING	Q15573.
PTM databases
PhosphoSite	Q15573.
Polymorphism databases
DMDM	74739864.
Proteomic databases
PRIDE	Q15573.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000352967; ENSP00000327072; ENSG00000143498.
GeneID	9015.
KEGG	hsa:9015.
UCSC	uc001hni.1. human. uc001hnj.1. human.
Organism-specific databases
CTD	9015.
GeneCards	GC01M222731.
HGNC	HGNC:11532. TAF1A.
MIM	604903. gene.
neXtProt	NX_Q15573.
PharmGKB	PA36307.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG12357.
GeneTree	ENSGT00390000011405.
HOGENOM	HBG713810.
HOVERGEN	HBG053462.
InParanoid	Q15573.
OMA	QIVPSHK.
PhylomeDB	Q15573.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
ArrayExpress	Q15573.
Bgee	Q15573.
CleanEx	HS_TAF1A.
Genevestigator	Q15573.
GermOnline	ENSG00000143498. Homo sapiens.
Family and domain databases
InterPro	IPR016629. RNA_pol_I_TAF1A/TAFI48. [Graphical view]
KO	K15212.
PIRSF	PIRSF015161. TAFI48. 1 hit.
ProtoNet	Search...
Other
NextBio	33775.
SOURCE	Search...

Entry information

Entry name

TAF1A_HUMAN

Accession

Primary (citable) accession number: Q15573
Secondary accession number(s): B2RDZ8, D3DTB7, Q9NWA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.