Q15562 (TEAD2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional enhancer factor TEF-4

Alternative name(s):
TEA domain family member 2
Short name=TEAD-2

Gene names

Name:	TEAD2
Synonyms:	TEF4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. Ref.6 Ref.7
Subunit structure	Interacts with YAP1 and WWTR1/TAZ. Ref.6 Ref.7
Subcellular location	Nucleus.
Sequence similarities	Contains 1 TEA DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryonic heart tube morphogenesis Inferred from electronic annotation. Source: Compara hippo signaling cascade Inferred from direct assay Ref.6Ref.7. Source: UniProtKB lateral mesoderm development Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from electronic annotation. Source: Compara neural tube closure Inferred from electronic annotation. Source: Compara notochord development Inferred from electronic annotation. Source: Compara paraxial mesoderm development Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome vasculogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	nucleoplasm Traceable author statement. Source: Reactome transcription factor complex Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.6Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15562-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15562-2)

The sequence of this isoform differs from the canonical sequence as follows:
     155-155: P → PQVV

Isoform 3 (identifier: Q15562-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     155-155: P → PQVV

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 447

447

Transcriptional enhancer factor TEF-4

PRO_0000205932

Regions

DNA binding

40 – 107

68

TEA

Region

172 – 447

276

Transcriptional activation Potential

Compositional bias

152 – 216

65

Pro-rich

Natural variations

Alternative sequence

1 – 131

131

Missing in isoform 3.

VSP_045126

Alternative sequence

155

1

P → PQVV in isoform 2 and isoform 3.

VSP_045127

Experimental info

Sequence conflict

144

1

P → L in CAA64214. Ref.5

Secondary structure

1

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447

Helix Strand Turn

Details...

Beta strand

229 – 238

10

Beta strand

249 – 255

7

Beta strand

268 – 270

3

Helix

271 – 277

7

Helix

285 – 291

7

Helix

294 – 296

3

Beta strand

297 – 304

8

Beta strand

326 – 337

12

Beta strand

340 – 349

10

Beta strand

352 – 362

11

Beta strand

364 – 366

3

Beta strand

369 – 378

10

Helix

381 – 390

10

Helix

396 – 403

8

Beta strand

406 – 414

9

Turn

415 – 417

3

Beta strand

420 – 430

11

Beta strand

433 – 435

3

Beta strand

438 – 445

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: A9077F55638A3AC7	FASTA	447	49,243
	10 20 30 40 50 60 MGEPRAGAAL DDGSGWTGSE EGSEEGTGGS EGAGGDGGPD AEGVWSPDIE QSFQEALAIY 70 80 90 100 110 120 PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSREIQSKLK 130 140 150 160 170 180 DQVSKDKAFQ TMATMSSAQL ISAPSLQAKL GPTGPQASEL FQFWSGGSGP PWNVPDVKPF 190 200 210 220 230 240 SQTPFTLSLT PPSTDLPGYE PPQALSPLPP PTPSPPAWQA RGLGTARLQL VEFSAFVEPP 250 260 270 280 290 300 DAVDSYQRHL FVHISQHCPS PGAPPLESVD VRQIYDKFPE KKGGLRELYD RGPPHAFFLV 310 320 330 340 350 360 KFWADLNWGP SGEEAGAGGS ISSGGFYGVS SQYESLEHMT LTCSSKVCSF GKQVVEKVET 370 380 390 400 410 420 ERAQLEDGRF VYRLLRSPMC EYLVNFLHKL RQLPERYMMN SVLENFTILQ VVTNRDTQEL 430 440 LLCTAYVFEV STSERGAQHH IYRLVRD
	Isoform 2 [UniParc]. Checksum: F90CF8D5E42E20E6	FASTA	450	49,569
	10 20 30 40 50 60 MGEPRAGAAL DDGSGWTGSE EGSEEGTGGS EGAGGDGGPD AEGVWSPDIE QSFQEALAIY 70 80 90 100 110 120 PPCGRRKIIL SDEGKMYGRN ELIARYIKLR TGKTRTRKQV SSHIQVLARR KSREIQSKLK 130 140 150 160 170 180 DQVSKDKAFQ TMATMSSAQL ISAPSLQAKL GPTGPQVVQA SELFQFWSGG SGPPWNVPDV 190 200 210 220 230 240 KPFSQTPFTL SLTPPSTDLP GYEPPQALSP LPPPTPSPPA WQARGLGTAR LQLVEFSAFV 250 260 270 280 290 300 EPPDAVDSYQ RHLFVHISQH CPSPGAPPLE SVDVRQIYDK FPEKKGGLRE LYDRGPPHAF 310 320 330 340 350 360 FLVKFWADLN WGPSGEEAGA GGSISSGGFY GVSSQYESLE HMTLTCSSKV CSFGKQVVEK 370 380 390 400 410 420 VETERAQLED GRFVYRLLRS PMCEYLVNFL HKLRQLPERY MMNSVLENFT ILQVVTNRDT 430 440 450 QELLLCTAYV FEVSTSERGA QHHIYRLVRD
	Isoform 3 [UniParc]. Checksum: 91BCE65CE4E8A7D1	FASTA	319	35,403
	10 20 30 40 50 60 MATMSSAQLI SAPSLQAKLG PTGPQVVQAS ELFQFWSGGS GPPWNVPDVK PFSQTPFTLS 70 80 90 100 110 120 LTPPSTDLPG YEPPQALSPL PPPTPSPPAW QARGLGTARL QLVEFSAFVE PPDAVDSYQR 130 140 150 160 170 180 HLFVHISQHC PSPGAPPLES VDVRQIYDKF PEKKGGLREL YDRGPPHAFF LVKFWADLNW 190 200 210 220 230 240 GPSGEEAGAG GSISSGGFYG VSSQYESLEH MTLTCSSKVC SFGKQVVEKV ETERAQLEDG 250 260 270 280 290 300 RFVYRLLRSP MCEYLVNFLH KLRQLPERYM MNSVLENFTI LQVVTNRDTQ ELLLCTAYVF 310 EVSTSERGAQ HHIYRLVRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Placenta and Testis.
[2]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Skin.
[5]	"A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain." Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I. J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-367. Tissue: Brain.
[6]	"TEAD mediates YAP-dependent gene induction and growth control." Zhao B., Ye X., Yu J., Li L., Li W., Li S., Yu J., Lin J.D., Wang C.Y., Chinnaiyan A.M., Lai Z.C., Guan K.L. Genes Dev. 22:1962-1971(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YAP1.
[7]	"TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L. J. Biol. Chem. 284:13355-13362(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH WWTR1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK093236 mRNA. Translation: BAC04104.1.
AK290736 mRNA. Translation: BAF83425.1.
AK300241 mRNA. Translation: BAG62008.1.
AC010524 Genomic DNA. No translation available.
CH471177 Genomic DNA. Translation: EAW52469.1.
BC051301 mRNA. Translation: AAH51301.1.
BC007556 mRNA. Translation: AAH07556.1.
X94440 mRNA. Translation: CAA64214.1.

IPI

IPI00018174.

PIR

G01116.

RefSeq

NP_001243587.1. NM_001256658.1.
NP_001243588.1. NM_001256659.1.
NP_001243589.1. NM_001256660.1.
NP_001243590.1. NM_001256661.1.
NP_001243591.1. NM_001256662.1.
NP_003589.1. NM_003598.1.

UniGene

Hs.515534.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3L15	X-ray	2.00	A/B	217-447	[»]

ProteinModelPortal

Q15562.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59318N.

IntAct

Q15562. 1 interaction.

STRING

9606.ENSP00000310701.

PTM databases

PhosphoSite

Q15562.

Polymorphism databases

DMDM

21264529.

Proteomic databases

PaxDb

Q15562.

PRIDE

Q15562.

Protocols and materials databases

DNASU

8463.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000311227; ENSP00000310701; ENSG00000074219.
ENST00000377214; ENSP00000366419; ENSG00000074219.
ENST00000539846; ENSP00000437928; ENSG00000074219.
ENST00000601519; ENSP00000469672; ENSG00000074219.

GeneID

8463.

KEGG

hsa:8463.

UCSC

uc002pnh.3. human.

Organism-specific databases

CTD

8463.

GeneCards

GC19M049843.

HGNC

HGNC:11715. TEAD2.

MIM

601729. gene.

neXtProt

NX_Q15562.

PharmGKB

PA36433.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG246992.

HOVERGEN

HBG056905.

InParanoid

Q15562.

KO

K09448.

OMA

TSERGAQ.

OrthoDB

EOG483D4Q.

PhylomeDB

Q15562.

Enzyme and pathway databases

Reactome

REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q15562.

Bgee

Q15562.

CleanEx

HS_TEAD2.

Genevestigator

Q15562.

GermOnline

ENSG00000074219. Homo sapiens.

Family and domain databases

InterPro

IPR000818. TEA/ATTS.
IPR016361. TEF.
[Graphical view]

PANTHER

PTHR11834. PTHR11834. 1 hit.

Pfam

PF01285. TEA. 1 hit.
[Graphical view]

PIRSF

PIRSF002603. TEF. 1 hit.

PRINTS

PR00065. TEADOMAIN.

SMART

SM00426. TEA. 1 hit.
[Graphical view]

PROSITE

PS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TEAD2. human.

EvolutionaryTrace

Q15562.

GenomeRNAi

8463.

NextBio

31676.

SOURCE

Search...

Entry information

Entry name

TEAD2_HUMAN

Accession

Primary (citable) accession number: Q15562
Secondary accession number(s): B4DTJ6, Q8NA25, Q96IG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 27, 2002
Last modified:	May 1, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.