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Q15560

- TCEA2_HUMAN

UniProt

Q15560 - TCEA2_HUMAN

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Protein

Transcription elongation factor A protein 2

Gene

TCEA2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri257 – 29741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. DNA-templated transcription, elongation Source: UniProtKB
  2. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  3. regulation of DNA-templated transcription, elongation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 2
Alternative name(s):
Testis-specific S-II
Transcription elongation factor S-II protein 2
Transcription elongation factor TFIIS.l
Gene namesi
Name:TCEA2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 20

Organism-specific databases

HGNCiHGNC:11614. TCEA2.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: HPA
  2. nucleus Source: HPA
  3. transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36373.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Transcription elongation factor A protein 2PRO_0000121449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki58 – 58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ15560.
PaxDbiQ15560.
PRIDEiQ15560.

PTM databases

PhosphoSiteiQ15560.

Expressioni

Tissue specificityi

Testis and ovary specific.1 Publication

Gene expression databases

BgeeiQ15560.
CleanExiHS_TCEA2.
ExpressionAtlasiQ15560. baseline and differential.
GenevestigatoriQ15560.

Organism-specific databases

HPAiHPA043836.

Interactioni

Subunit structurei

Interacts with the basal transcription factor GTF2B. Interacts with REXO1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MAGEA11P433642EBI-710310,EBI-739552
MARK3P274482EBI-710310,EBI-707595

Protein-protein interaction databases

BioGridi112781. 32 interactions.
IntActiQ15560. 16 interactions.
STRINGi9606.ENSP00000343515.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi139 – 15113Combined sources
Helixi155 – 1595Combined sources
Helixi163 – 17816Combined sources
Turni181 – 1833Combined sources
Helixi184 – 19714Combined sources
Beta strandi199 – 2024Combined sources
Helixi203 – 2097Combined sources
Helixi215 – 2206Combined sources
Helixi223 – 2275Combined sources
Turni229 – 2335Combined sources
Beta strandi235 – 2373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LW4NMR-A130-239[»]
ProteinModelPortaliQ15560.
SMRiQ15560. Positions 5-93, 130-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 8378TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini138 – 254117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri257 – 29741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1594.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiQ15560.
KOiK03145.
OMAiGADCEHI.
OrthoDBiEOG7GQXWQ.
PhylomeDBiQ15560.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q15560-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMGKEEEIAR IARRLDKMVT KKSAEGAMDL LRELKAMPIT LHLLQSTRVG
60 70 80 90 100
MSVNALRKQS SDEEVIALAK SLIKSWKKLL DASDAKARER GRGMPLPTSS
110 120 130 140 150
RDASEAPDPS RKRPELPRAP STPRITTFPP VPVTCDAVRN KCREMLTAAL
160 170 180 190 200
QTDHDHVAIG ADCERLSAQI EECIFRDVGN TDMKYKNRVR SRISNLKDAK
210 220 230 240 250
NPDLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTK EAIREHQMAR
260 270 280 290
TGGTQTDLFT CGKCRKKNCT YTQVQTRSSD EPMTTFVVCN ECGNRWKFC
Length:299
Mass (Da):33,601
Last modified:November 1, 1996 - v1
Checksum:iFF0EAE8EDC7BCBE9
GO
Isoform 2 (identifier: Q15560-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Note: No experimental confirmation available.

Show »
Length:272
Mass (Da):30,569
Checksum:iB0023436A8268207
GO

Sequence cautioni

The sequence CAD11900.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAD11901.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551A → T in BAG51383. (PubMed:14702039)Curated
Sequence conflicti164 – 1641E → V in BAG51383. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2727Missing in isoform 2. 1 PublicationVSP_047345Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50495 mRNA. Translation: BAA09089.1.
AK027824 mRNA. Translation: BAG51383.1.
AL590548 Genomic DNA. Translation: CAD11900.1. Sequence problems.
AL590548 Genomic DNA. Translation: CAD11901.1. Sequence problems.
BC018896 mRNA. Translation: AAH18896.1.
U86749 Genomic DNA. Translation: AAC39553.1.
CCDSiCCDS13553.1. [Q15560-1]
CCDS13554.1. [Q15560-2]
PIRiJC4577.
RefSeqiNP_003186.1. NM_003195.4. [Q15560-1]
NP_942016.1. NM_198723.1. [Q15560-2]
XP_006723937.1. XM_006723874.1. [Q15560-2]
UniGeneiHs.505004.
Hs.736326.

Genome annotation databases

EnsembliENST00000343484; ENSP00000343515; ENSG00000171703. [Q15560-1]
ENST00000361317; ENSP00000354552; ENSG00000171703. [Q15560-2]
GeneIDi6919.
KEGGihsa:6919.
UCSCiuc021wgp.1. human. [Q15560-1]

Polymorphism databases

DMDMi28380177.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50495 mRNA. Translation: BAA09089.1 .
AK027824 mRNA. Translation: BAG51383.1 .
AL590548 Genomic DNA. Translation: CAD11900.1 . Sequence problems.
AL590548 Genomic DNA. Translation: CAD11901.1 . Sequence problems.
BC018896 mRNA. Translation: AAH18896.1 .
U86749 Genomic DNA. Translation: AAC39553.1 .
CCDSi CCDS13553.1. [Q15560-1 ]
CCDS13554.1. [Q15560-2 ]
PIRi JC4577.
RefSeqi NP_003186.1. NM_003195.4. [Q15560-1 ]
NP_942016.1. NM_198723.1. [Q15560-2 ]
XP_006723937.1. XM_006723874.1. [Q15560-2 ]
UniGenei Hs.505004.
Hs.736326.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LW4 NMR - A 130-239 [» ]
ProteinModelPortali Q15560.
SMRi Q15560. Positions 5-93, 130-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112781. 32 interactions.
IntActi Q15560. 16 interactions.
STRINGi 9606.ENSP00000343515.

PTM databases

PhosphoSitei Q15560.

Polymorphism databases

DMDMi 28380177.

Proteomic databases

MaxQBi Q15560.
PaxDbi Q15560.
PRIDEi Q15560.

Protocols and materials databases

DNASUi 6919.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000343484 ; ENSP00000343515 ; ENSG00000171703 . [Q15560-1 ]
ENST00000361317 ; ENSP00000354552 ; ENSG00000171703 . [Q15560-2 ]
GeneIDi 6919.
KEGGi hsa:6919.
UCSCi uc021wgp.1. human. [Q15560-1 ]

Organism-specific databases

CTDi 6919.
GeneCardsi GC20P062681.
H-InvDB HIX0174737.
HGNCi HGNC:11614. TCEA2.
HPAi HPA043836.
MIMi 604784. gene.
neXtProti NX_Q15560.
PharmGKBi PA36373.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1594.
GeneTreei ENSGT00390000017794.
HOGENOMi HOG000195015.
HOVERGENi HBG055022.
InParanoidi Q15560.
KOi K03145.
OMAi GADCEHI.
OrthoDBi EOG7GQXWQ.
PhylomeDBi Q15560.
TreeFami TF314970.

Miscellaneous databases

GeneWikii TCEA2.
GenomeRNAii 6919.
NextBioi 27067.
PROi Q15560.
SOURCEi Search...

Gene expression databases

Bgeei Q15560.
CleanExi HS_TCEA2.
ExpressionAtlasi Q15560. baseline and differential.
Genevestigatori Q15560.

Family and domain databases

Gene3Di 1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProi IPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view ]
Pfami PF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view ]
PIRSFi PIRSF006704. TF_IIS. 1 hit.
SMARTi SM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view ]
SUPFAMi SSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsi TIGR01385. TFSII. 1 hit.
PROSITEi PS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II."
    Umehara T., Kida S., Yamamoto T., Horikoshi M.
    Gene 167:297-302(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GTF2B.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Placenta.
  3. "The DNA sequence and comparative analysis of human chromosome 20."
    Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E.
    , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
    Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  5. "Genomic characterization of a testis-specific TFIIS (TCEA2) gene."
    Weaver Z.A., Kane C.M.
    Genomics 46:516-519(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
    Tissue: Brain.
  6. "RNA polymerase II complexes in the very early phase of transcription are not susceptible to TFIIS-induced exonucleolytic cleavage."
    Sijbrandi R., Fiedler U., Timmers H.T.M.
    Nucleic Acids Res. 30:2290-2298(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Identification of EloA-BP1, a novel Elongin A binding protein with an exonuclease homology domain."
    Tamura K., Miyata K., Sugahara K., Onishi S., Shuin T., Aso T.
    Biochem. Biophys. Res. Commun. 309:189-195(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REXO1.
  8. "Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells."
    Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X.
    Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-58.
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTCEA2_HUMAN
AccessioniPrimary (citable) accession number: Q15560
Secondary accession number(s): B3KNM1, Q8TD37, Q8TD38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3