Q15560 (TCEA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor A protein 2

Alternative name(s):
Testis-specific S-II
Transcription elongation factor S-II protein 2
Transcription elongation factor TFIIS.l

Gene names

Name:

TCEA2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Ref.5
Subunit structure	Interacts with the basal transcription factor GTF2B. Interacts with REXO1. Ref.1 Ref.6
Subcellular location	Nucleus.
Tissue specificity	Testis and ovary specific. Ref.1
Sequence similarities	Belongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.
Caution	It is uncertain whether Met-1 or Met-2 is the initiator.
Sequence caution	The sequence CAD11900.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAD11901.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, elongation Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of DNA-dependent transcription, elongation Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	centrosome Inferred from direct assay. Source: HPA transcription elongation factor complex Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAGEA11	P43364	2	EBI-710310,EBI-739552
MARK3	P27448	2	EBI-710310,EBI-707595

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 299

299

Transcription elongation factor A protein 2

PRO_0000121449

Regions

Domain

6 – 83

78

TFIIS N-terminal

Domain

138 – 254

117

TFIIS central

Zinc finger

257 – 297

41

TFIIS-type

Amino acid modifications

Cross-link

58

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Secondary structure

1

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299

Helix Strand Turn

Details...

Helix

139 – 151

13

Helix

155 – 159

5

Helix

163 – 178

16

Turn

181 – 183

3

Helix

184 – 197

14

Beta strand

199 – 202

4

Helix

203 – 209

7

Helix

215 – 220

6

Helix

223 – 227

5

Turn

229 – 233

5

Beta strand

235 – 237

3

Sequences

	Sequence		Length	Mass (Da)
	Q15560 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FF0EAE8EDC7BCBE9	FASTA	299	33,601
	10 20 30 40 50 60 MMGKEEEIAR IARRLDKMVT KKSAEGAMDL LRELKAMPIT LHLLQSTRVG MSVNALRKQS 70 80 90 100 110 120 SDEEVIALAK SLIKSWKKLL DASDAKARER GRGMPLPTSS RDASEAPDPS RKRPELPRAP 130 140 150 160 170 180 STPRITTFPP VPVTCDAVRN KCREMLTAAL QTDHDHVAIG ADCERLSAQI EECIFRDVGN 190 200 210 220 230 240 TDMKYKNRVR SRISNLKDAK NPDLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTK 250 260 270 280 290 EAIREHQMAR TGGTQTDLFT CGKCRKKNCT YTQVQTRSSD EPMTTFVVCN ECGNRWKFC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II." Umehara T., Kida S., Yamamoto T., Horikoshi M. Gene 167:297-302(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INTERACTION WITH GTF2B.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Genomic characterization of a testis-specific TFIIS (TCEA2) gene." Weaver Z.A., Kane C.M. Genomics 46:516-519(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24. Tissue: Brain.
[5]	"RNA polymerase II complexes in the very early phase of transcription are not susceptible to TFIIS-induced exonucleolytic cleavage." Sijbrandi R., Fiedler U., Timmers H.T.M. Nucleic Acids Res. 30:2290-2298(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Identification of EloA-BP1, a novel Elongin A binding protein with an exonuclease homology domain." Tamura K., Miyata K., Sugahara K., Onishi S., Shuin T., Aso T. Biochem. Biophys. Res. Commun. 309:189-195(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH REXO1.
[7]	"Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells." Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X. Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-58, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50495 mRNA. Translation: BAA09089.1.
AL590548 Genomic DNA. Translation: CAD11900.1. Sequence problems.
AL590548 Genomic DNA. Translation: CAD11901.1. Sequence problems.
BC018896 mRNA. Translation: AAH18896.1.
U86749 Genomic DNA. Translation: AAC39553.1.

IPI

IPI00018403.

PIR

JC4577.

RefSeq

NP_003186.1. NM_003195.4.
NP_942016.1. NM_198723.1.

UniGene

Hs.505004.
Hs.736326.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LW4	NMR	-	A	130-239	[»]

ProteinModelPortal

Q15560.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15560. 15 interactions.

STRING

9606.ENSP00000343515.

PTM databases

PhosphoSite

Q15560.

Polymorphism databases

DMDM

28380177.

Proteomic databases

PaxDb

Q15560.

PRIDE

Q15560.

Protocols and materials databases

DNASU

6919.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000343484; ENSP00000343515; ENSG00000171703.
ENST00000361317; ENSP00000354552; ENSG00000171703.
ENST00000458442; ENSP00000416026; ENSG00000171703.

GeneID

6919.

KEGG

hsa:6919.

UCSC

uc021wgp.1. human.

Organism-specific databases

CTD

6919.

GeneCards

GC20P062681.

H-InvDB

HIX0174737.

HGNC

HGNC:11614. TCEA2.

HPA

HPA043836.

MIM

604784. gene.

neXtProt

NX_Q15560.

PharmGKB

PA36373.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1594.

HOGENOM

HOG000195015.

HOVERGEN

HBG055022.

KO

K03145.

OMA

AVGADCE.

Gene expression databases

ArrayExpress

Q15560.

Bgee

Q15560.

CleanEx

HS_TCEA2.

Genevestigator

Q15560.

GermOnline

ENSG00000171703. Homo sapiens.

Family and domain databases

Gene3D

1.10.472.30. 1 hit.
1.20.930.10. 1 hit.

InterPro

IPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]

Pfam

PF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]

PIRSF

PIRSF006704. TF_IIS. 1 hit.

SMART

SM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]

SUPFAM

SSF46942. TFIIS_centre. 1 hit.
SSF47676. TFIIS_conserved. 1 hit.

TIGRFAMs

TIGR01385. TFSII. 1 hit.

PROSITE

PS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

6919.

NextBio

27067.

SOURCE

Search...

Entry information

Entry name

TCEA2_HUMAN

Accession

Primary (citable) accession number: Q15560
Secondary accession number(s): Q8TD37, Q8TD38

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.