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Q15560 (TCEA2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription elongation factor A protein 2
Alternative name(s):
Testis-specific S-II
Transcription elongation factor S-II protein 2
Transcription elongation factor TFIIS.l
Gene names
Name:TCEA2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Ref.6

Subunit structure

Interacts with the basal transcription factor GTF2B. Interacts with REXO1. Ref.1 Ref.7

Subcellular location

Nucleus.

Tissue specificity

Testis and ovary specific. Ref.1

Sequence similarities

Belongs to the TFS-II family.

Contains 1 TFIIS central domain.

Contains 1 TFIIS N-terminal domain.

Contains 1 TFIIS-type zinc finger.

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.

Sequence caution

The sequence CAD11900.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAD11901.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAGEA11P433642EBI-710310,EBI-739552
MARK3P274482EBI-710310,EBI-707595

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15560-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15560-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Transcription elongation factor A protein 2
PRO_0000121449

Regions

Domain6 – 8378TFIIS N-terminal
Domain138 – 254117TFIIS central
Zinc finger257 – 29741TFIIS-type

Amino acid modifications

Cross-link58Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.8

Natural variations

Alternative sequence1 – 2727Missing in isoform 2.
VSP_047345

Experimental info

Sequence conflict551A → T in BAG51383. Ref.2
Sequence conflict1641E → V in BAG51383. Ref.2

Secondary structure

..................... 299
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FF0EAE8EDC7BCBE9

FASTA29933,601
        10         20         30         40         50         60 
MMGKEEEIAR IARRLDKMVT KKSAEGAMDL LRELKAMPIT LHLLQSTRVG MSVNALRKQS 

        70         80         90        100        110        120 
SDEEVIALAK SLIKSWKKLL DASDAKARER GRGMPLPTSS RDASEAPDPS RKRPELPRAP 

       130        140        150        160        170        180 
STPRITTFPP VPVTCDAVRN KCREMLTAAL QTDHDHVAIG ADCERLSAQI EECIFRDVGN 

       190        200        210        220        230        240 
TDMKYKNRVR SRISNLKDAK NPDLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTK 

       250        260        270        280        290 
EAIREHQMAR TGGTQTDLFT CGKCRKKNCT YTQVQTRSSD EPMTTFVVCN ECGNRWKFC 

« Hide

Isoform 2 [UniParc].

Checksum: B0023436A8268207
Show »

FASTA27230,569

References

« Hide 'large scale' references
[1]"Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II."
Umehara T., Kida S., Yamamoto T., Horikoshi M.
Gene 167:297-302(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH GTF2B.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta.
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Skin.
[5]"Genomic characterization of a testis-specific TFIIS (TCEA2) gene."
Weaver Z.A., Kane C.M.
Genomics 46:516-519(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
Tissue: Brain.
[6]"RNA polymerase II complexes in the very early phase of transcription are not susceptible to TFIIS-induced exonucleolytic cleavage."
Sijbrandi R., Fiedler U., Timmers H.T.M.
Nucleic Acids Res. 30:2290-2298(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Identification of EloA-BP1, a novel Elongin A binding protein with an exonuclease homology domain."
Tamura K., Miyata K., Sugahara K., Onishi S., Shuin T., Aso T.
Biochem. Biophys. Res. Commun. 309:189-195(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH REXO1.
[8]"Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells."
Tan F., Lu L., Cai Y., Wang J., Xie Y., Wang L., Gong Y., Xu B.-E., Wu J., Luo Y., Qiang B., Yuan J., Sun X., Peng X.
Proteomics 8:2885-2896(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-58.
Tissue: Cervix carcinoma.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50495 mRNA. Translation: BAA09089.1.
AK027824 mRNA. Translation: BAG51383.1.
AL590548 Genomic DNA. Translation: CAD11900.1. Sequence problems.
AL590548 Genomic DNA. Translation: CAD11901.1. Sequence problems.
BC018896 mRNA. Translation: AAH18896.1.
U86749 Genomic DNA. Translation: AAC39553.1.
CCDSCCDS13553.1. [Q15560-1]
CCDS13554.1. [Q15560-2]
PIRJC4577.
RefSeqNP_003186.1. NM_003195.4. [Q15560-1]
NP_942016.1. NM_198723.1. [Q15560-2]
XP_006723937.1. XM_006723874.1. [Q15560-2]
UniGeneHs.505004.
Hs.736326.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LW4NMR-A130-239[»]
ProteinModelPortalQ15560.
SMRQ15560. Positions 2-86, 130-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112781. 32 interactions.
IntActQ15560. 16 interactions.
STRING9606.ENSP00000343515.

PTM databases

PhosphoSiteQ15560.

Polymorphism databases

DMDM28380177.

Proteomic databases

MaxQBQ15560.
PaxDbQ15560.
PRIDEQ15560.

Protocols and materials databases

DNASU6919.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000343484; ENSP00000343515; ENSG00000171703. [Q15560-1]
ENST00000361317; ENSP00000354552; ENSG00000171703. [Q15560-2]
GeneID6919.
KEGGhsa:6919.
UCSCuc021wgp.1. human. [Q15560-1]

Organism-specific databases

CTD6919.
GeneCardsGC20P062681.
H-InvDBHIX0174737.
HGNCHGNC:11614. TCEA2.
HPAHPA043836.
MIM604784. gene.
neXtProtNX_Q15560.
PharmGKBPA36373.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1594.
HOGENOMHOG000195015.
HOVERGENHBG055022.
KOK03145.
OMAGADCEHI.
OrthoDBEOG7GQXWQ.
PhylomeDBQ15560.
TreeFamTF314970.

Gene expression databases

ArrayExpressQ15560.
BgeeQ15560.
CleanExHS_TCEA2.
GenevestigatorQ15560.

Family and domain databases

Gene3D1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR017890. TFS2M.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFPIRSF006704. TF_IIS. 1 hit.
SMARTSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsTIGR01385. TFSII. 1 hit.
PROSITEPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiTCEA2.
GenomeRNAi6919.
NextBio27067.
PROQ15560.
SOURCESearch...

Entry information

Entry nameTCEA2_HUMAN
AccessionPrimary (citable) accession number: Q15560
Secondary accession number(s): B3KNM1, Q8TD37, Q8TD38
Entry history
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM