Q15544 (TAF11_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 11

Alternative name(s):
TFIID subunit p30-beta
Transcription initiation factor TFIID 28 kDa subunit
Short name=TAF(II)28
Short name=TAFII-28
Short name=TAFII28

Gene names

Name:	TAF11
Synonyms:	TAF2I
ORF Names:	PRO2134

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core TAFII present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).
Subunit structure	TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF13 both in vitro and intracellularly; also interacts directly with TBP.
Subcellular location	Nucleus.
Domain	TBP and TAFII18 bind to distinct domains of TAFII28.
Sequence similarities	Belongs to the TAF11 family.
Sequence caution	The sequence AAF22038.1 differs from that shown. Reason: Frameshift at position 208.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation by host of viral transcription Inferred from direct assay PubMed 9108034. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Inferred by curator Ref.1. Source: UniProtKB viral reproduction Traceable author statement. Source: Reactome
Cellular_component	transcription factor TFIID complex Inferred from direct assay Ref.1PubMed 8670810. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: Compara transcription coactivator activity Inferred from direct assay PubMed 8670810. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TAF13	Q15543	2	EBI-1027005,EBI-1026992

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15544-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15544-2)

The sequence of this isoform differs from the canonical sequence as follows:
     137-211: LIQSITGTSV...NSKHKKIIFF → HWMCVRSGEKCHHYNPNI

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 211

211

Transcription initiation factor TFIID subunit 11

PRO_0000118902

Amino acid modifications

Modified residue

1

1

N-acetylmethionine Ref.11

Natural variations

Alternative sequence

137 – 211

75

LIQSI…KIIFF → HWMCVRSGEKCHHYNPNI in isoform 2.

VSP_044936

Natural variant

68

1

T → R. Ref.6
Corresponds to variant rs15922 [ dbSNP | Ensembl ].

VAR_016333

Natural variant

155

1

S → F.
Corresponds to variant rs11537996 [ dbSNP | Ensembl ].

VAR_052261

Secondary structure

1

.

.

.

.

.

.

.

.

.

211

Helix Strand Turn

Details...

Helix

115 – 126

12

Helix

131 – 142

12

Helix

148 – 175

28

Helix

184 – 196

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 77C7BDC667D19A8F	FASTA	211	23,307
	10 20 30 40 50 60 MDDAHESPSD KGGETGESDE TAAVPGDPGA TDTDGIPEET DGDADVDLKE AAAEEGELES 70 80 90 100 110 120 QDVSDLTTVE REDSSLLNPA AKKLKIDTKE KKEKKQKVDE DEIQKMQILV SSFSEEQLNR 130 140 150 160 170 180 YEMYRRSAFP KAAIKRLIQS ITGTSVSQNV VIAMSGISKV FVGEVVEEAL DVCEKWGEMP 190 200 210 PLQPKHMREA VRRLKSKGQI PNSKHKKIIF F
	Isoform 2 [UniParc]. Checksum: 73B2AE01BABD971B	FASTA	154	17,142
	10 20 30 40 50 60 MDDAHESPSD KGGETGESDE TAAVPGDPGA TDTDGIPEET DGDADVDLKE AAAEEGELES 70 80 90 100 110 120 QDVSDLTTVE REDSSLLNPA AKKLKIDTKE KKEKKQKVDE DEIQKMQILV SSFSEEQLNR 130 140 150 YEMYRRSAFP KAAIKRHWMC VRSGEKCHHY NPNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of hTAFII18, hTAFII20 and hTAFII28: three subunits of the human transcription factor TFIID." Mengus G., May M., Jacq X., Staub A., Tora L., Chambon P., Davidson I. EMBO J. 14:1520-1531(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]	"Isolation and characterization of a cDNA encoding a human TFIID subunit containing a variety of putative structural motifs including direct repeats." Kuzuhara T., Horikoshi M. Biol. Pharm. Bull. 19:122-126(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Subthalamic nucleus and Testis.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]	NIEHS SNPs program Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-68.
[7]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[9]	"Functional prediction of the coding sequences of 33 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Bi J., Zhang Y., Liu M., He F. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-211 (ISOFORM 1). Tissue: Fetal liver.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family." Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D. Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 113-201 IN COMPLEX WITH TAF13.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X83928 mRNA. Translation: CAA58780.1.
D63705 mRNA. Translation: BAA23620.1.
AK302226 mRNA. Translation: BAG63580.1.
AK313475 mRNA. Translation: BAG36260.1.
CR542068 mRNA. Translation: CAG46865.1.
BT019404 mRNA. Translation: AAV38211.1.
BT019405 mRNA. Translation: AAV38212.1.
AY217767 Genomic DNA. Translation: AAO25652.1.
AL033520 Genomic DNA. Translation: CAB96725.1.
BC021972 mRNA. Translation: AAH21972.1.
AF118094 mRNA. Translation: AAF22038.1. Sequence problems.

IPI

IPI00018110.
IPI00910537.

PIR

JC4540.

RefSeq

NP_001257417.1. NM_001270488.1.
NP_005634.1. NM_005643.3.

UniGene

Hs.112444.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BH8	X-ray	3.00	B	113-201	[»]
1BH9	X-ray	2.60	B	113-201	[»]

ProteinModelPortal

Q15544.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-495N.

IntAct

Q15544. 4 interactions.

STRING

9606.ENSP00000354633.

PTM databases

PhosphoSite

Q15544.

Polymorphism databases

DMDM

3024707.

Proteomic databases

PaxDb

Q15544.

PRIDE

Q15544.

Protocols and materials databases

DNASU

6882.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361288; ENSP00000354633; ENSG00000064995.
ENST00000420584; ENSP00000408121; ENSG00000064995.

GeneID

6882.

KEGG

hsa:6882.

UCSC

uc003ojw.1. human.

Organism-specific databases

CTD

6882.

GeneCards

GC06M034892.

HGNC

HGNC:11544. TAF11.

MIM

600772. gene.

neXtProt

NX_Q15544.

PharmGKB

PA36319.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5251.

HOGENOM

HOG000006355.

HOVERGEN

HBG079228.

InParanoid

Q15544.

KO

K03135.

OMA

DDAHESP.

OrthoDB

EOG4B8JFB.

PhylomeDB

Q15544.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

Q15544.

Bgee

Q15544.

CleanEx

HS_TAF11.

Genevestigator

Q15544.

GermOnline

ENSG00000064995. Homo sapiens.

Family and domain databases

Gene3D

1.10.20.10. 1 hit.

InterPro

IPR009072. Histone-fold.
IPR006809. TAFII28.
[Graphical view]

PANTHER

PTHR13218. PTHR13218. 1 hit.

Pfam

PF04719. TAFII28. 1 hit.
[Graphical view]

SUPFAM

SSF47113. Histone-fold. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q15544.

GenomeRNAi

6882.

NextBio

26887.

SOURCE

Search...

Entry information

Entry name

TAF11_HUMAN

Accession

Primary (citable) accession number: Q15544
Secondary accession number(s): B2R8R3, B4DY18, Q9UHS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.