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Protein

Transcription initiation factor TFIID subunit 5

Gene

TAF5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.

GO - Molecular functioni

  • DNA binding transcription factor activity Source: UniProtKB
  • identical protein binding Source: IntAct
  • protein dimerization activity Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SignaLinkiQ15542

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 5
Alternative name(s):
Transcription initiation factor TFIID 100 kDa subunit
Short name:
TAF(II)100
Short name:
TAFII-100
Short name:
TAFII100
Gene namesi
Name:TAF5
Synonyms:TAF2D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000148835.10
HGNCiHGNC:11539 TAF5
MIMi601787 gene
neXtProtiNX_Q15542

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000148835
PharmGKBiPA36314

Polymorphism and mutation databases

BioMutaiTAF5
DMDMi78103206

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512571 – 800Transcription initiation factor TFIID subunit 5Add BLAST800

Proteomic databases

EPDiQ15542
MaxQBiQ15542
PaxDbiQ15542
PeptideAtlasiQ15542
PRIDEiQ15542
TopDownProteomicsiQ15542-2 [Q15542-2]

PTM databases

iPTMnetiQ15542
PhosphoSitePlusiQ15542

Expressioni

Gene expression databases

BgeeiENSG00000148835
CleanExiHS_TAF5
GenevisibleiQ15542 HS

Organism-specific databases

HPAiHPA006195
HPA006474

Interactioni

Subunit structurei

Homodimer. TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP. Interacts with SV40 Large T antigen.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-1560145,EBI-1560145

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi112740, 45 interactors
CORUMiQ15542
DIPiDIP-28150N
IntActiQ15542, 20 interactors
MINTiQ15542
STRINGi9606.ENSP00000358854

Structurei

Secondary structure

1800
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi198 – 202Combined sources5
Helixi203 – 205Combined sources3
Helixi214 – 226Combined sources13
Helixi230 – 236Combined sources7
Helixi237 – 239Combined sources3
Helixi240 – 253Combined sources14
Helixi257 – 267Combined sources11
Helixi268 – 270Combined sources3
Helixi273 – 275Combined sources3
Helixi276 – 283Combined sources8
Helixi288 – 291Combined sources4
Helixi295 – 300Combined sources6
Helixi302 – 304Combined sources3
Beta strandi305 – 310Combined sources6
Helixi311 – 321Combined sources11
Helixi328 – 335Combined sources8
Beta strandi338 – 342Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NXPX-ray2.17A/B/C/D/E/F/G/H188-343[»]
ProteinModelPortaliQ15542
SMRiQ15542
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15542

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 124LisHPROSITE-ProRule annotationAdd BLAST33
Repeati468 – 507WD 1Add BLAST40
Repeati541 – 580WD 2Add BLAST40
Repeati583 – 624WD 3Add BLAST42
Repeati625 – 666WD 4Add BLAST42
Repeati667 – 706WD 5Add BLAST40
Repeati709 – 748WD 6Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 49Poly-Gly4
Compositional biasi399 – 407Poly-Lys9

Domaini

Distinct domains of TAF5/TAFII100 are required for functional interaction with transcription factor TFIIFB (RAP30) and incorporation into the TFIID complex.

Sequence similaritiesi

Belongs to the WD repeat TAF5 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0263 Eukaryota
ENOG410XPM5 LUCA
GeneTreeiENSGT00910000144058
HOVERGENiHBG050226
InParanoidiQ15542
KOiK03130
OMAiDCVQFHP
OrthoDBiEOG091G03SP
PhylomeDBiQ15542
TreeFamiTF300669

Family and domain databases

CDDicd08044 TAF5_NTD2, 1 hit
Gene3Di1.25.40.500, 1 hit
2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR006594 LisH
IPR007582 TFIID_NTD2
IPR037264 TFIID_NTD2_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF04494 TFIID_NTD2, 1 hit
PF00400 WD40, 6 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF160897 SSF160897, 1 hit
SSF50978 SSF50978, 2 hits
PROSITEiView protein in PROSITE
PS50896 LISH, 1 hit
PS00678 WD_REPEATS_1, 3 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q15542-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN
60 70 80 90 100
VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAGA PHDRQTLLAV
110 120 130 140 150
LQFLRQSKLR EAEEALRREA GLLEEAVAGS GAPGEVDSAG AEVTSALLSR
160 170 180 190 200
VTASAPGPAA PDPPGTGASG ATVVSGSASG PAAPGKVGSV AVEDQPDVSA
210 220 230 240 250
VLSAYNQQGD PTMYEEYYSG LKHFIECSLD CHRAELSQLF YPLFVHMYLE
260 270 280 290 300
LVYNQHENEA KSFFEKFHGD QECYYQDDLR VLSSLTKKEH MKGNETMLDF
310 320 330 340 350
RTSKFVLRIS RDSYQLLKRH LQEKQNNQIW NIVQEHLYID IFDGMPRSKQ
360 370 380 390 400
QIDAMVGSLA GEAKREANKS KVFFGLLKEP EIEVPLDDED EEGENEEGKP
410 420 430 440 450
KKKKPKKDSI GSKSKKQDPN APPQNRIPLP ELKDSDKLDK IMNMKETTKR
460 470 480 490 500
VRLGPDCLPS ICFYTFLNAY QGLTAVDVTD DSSLIAGGFA DSTVRVWSVT
510 520 530 540 550
PKKLRSVKQA SDLSLIDKES DDVLERIMDE KTASELKILY GHSGPVYGAS
560 570 580 590 600
FSPDRNYLLS SSEDGTVRLW SLQTFTCLVG YKGHNYPVWD TQFSPYGYYF
610 620 630 640 650
VSGGHDRVAR LWATDHYQPL RIFAGHLADV NCTRFHPNSN YVATGSADRT
660 670 680 690 700
VRLWDVLNGN CVRIFTGHKG PIHSLTFSPN GRFLATGATD GRVLLWDIGH
710 720 730 740 750
GLMVGELKGH TDTVCSLRFS RDGEILASGS MDNTVRLWDA IKAFEDLETD
760 770 780 790 800
DFTTATGHIN LPENSQELLL GTYMTKSTPV VHLHFTRRNL VLAAGAYSPQ
Length:800
Mass (Da):86,830
Last modified:May 10, 2005 - v3
Checksum:i87A178BFB7071992
GO
Isoform Short (identifier: Q15542-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     556-610: Missing.

Show »
Length:745
Mass (Da):80,508
Checksum:iE97ADB7163F99617
GO

Sequence cautioni

The sequence AAC50902 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD97335 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126A → T in CAA64777 (PubMed:8758937).Curated1
Sequence conflicti126A → T in BAD97335 (Ref. 5) Curated1
Sequence conflicti300F → L in CAA64777 (PubMed:8758937).Curated1
Sequence conflicti455P → S in CAA64777 (PubMed:8758937).Curated1
Sequence conflicti469A → V in CAA64777 (PubMed:8758937).Curated1
Sequence conflicti778Missing in CAA64777 (PubMed:8758937).Curated1
Sequence conflicti787R → L in AAH52268 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018462130S → A4 PublicationsCorresponds to variant dbSNP:rs10883859Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006787556 – 610Missing in isoform Short. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95525 mRNA Translation: CAA64777.1
U75309 mRNA Translation: AAC50902.1 Different initiation.
U80191 mRNA Translation: AAC51215.1
AK223615 mRNA Translation: BAD97335.1 Different initiation.
AK291229 mRNA Translation: BAF83918.1
AL591408 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49645.1
CH471066 Genomic DNA Translation: EAW49646.1
BC052268 mRNA Translation: AAH52268.2
BC136340 mRNA Translation: AAI36341.1
BC136348 mRNA Translation: AAI36349.1
CCDSiCCDS7547.1 [Q15542-1]
RefSeqiNP_008882.2, NM_006951.4 [Q15542-1]
NP_620640.1, NM_139052.2 [Q15542-2]
UniGeneiHs.96103

Genome annotation databases

EnsembliENST00000369839; ENSP00000358854; ENSG00000148835 [Q15542-1]
GeneIDi6877
KEGGihsa:6877
UCSCiuc001kwv.5 human [Q15542-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTAF5_HUMAN
AccessioniPrimary (citable) accession number: Q15542
Secondary accession number(s): A8K5B4
, B2RMR0, B7ZKJ6, Q53EM4, Q5SYD5, Q86UZ7, Q9Y4K5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 182 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health