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Q15532

- SSXT_HUMAN

UniProt

Q15532 - SSXT_HUMAN

Protein

Protein SSXT

Gene

SS18

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei366 – 3672Breakpoint for translocation to form the SSXT-SSX1 fusion protein (rare)
    Sitei410 – 4112Breakpoint for translocation to form the SSXT-SSX1 or SSXT-SSX2 fusion proteins

    GO - Molecular functioni

    1. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. cell morphogenesis Source: Ensembl
    2. ephrin receptor signaling pathway Source: Ensembl
    3. microtubule cytoskeleton organization Source: Ensembl
    4. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    5. response to drug Source: Ensembl
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SSXT
    Alternative name(s):
    Protein SYT
    Synovial sarcoma translocated to X chromosome protein
    Gene namesi
    Name:SS18
    Synonyms:SSXT, SYT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:11340. SS18.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytoplasmic microtubule Source: Ensembl
    2. nucleus Source: ProtInc

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).

    Keywords - Diseasei

    Proto-oncogene

    Organism-specific databases

    Orphaneti3273. Synovial sarcoma.
    PharmGKBiPA36164.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 418417Protein SSXTPRO_0000181823Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ15532.
    PaxDbiQ15532.
    PRIDEiQ15532.

    PTM databases

    PhosphoSiteiQ15532.

    Expressioni

    Tissue specificityi

    Fairly ubiquitously expressed. Expressed in synovial sarcomas and in other human cell lines. The fusion genes SSXT-SSX1 and SSXT-SSX2 are expressed only in synovial sarcomas.

    Gene expression databases

    ArrayExpressiQ15532.
    BgeeiQ15532.
    CleanExiHS_SS18.
    GenevestigatoriQ15532.

    Organism-specific databases

    HPAiHPA055741.
    HPA059539.

    Interactioni

    Subunit structurei

    Interacts with MLLT10. Isoform 1 interacts with RBM14 isoform 1. Isoform 2 interacts with RBM14 isoform 1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATF2P153362EBI-2560599,EBI-1170906

    Protein-protein interaction databases

    BioGridi112638. 21 interactions.
    IntActiQ15532. 16 interactions.
    STRINGi9606.ENSP00000269138.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15532.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati344 – 356131Add
    BLAST
    Repeati357 – 369132Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 186185Transcriptional activationAdd
    BLAST
    Regioni344 – 369262 X 13 AA imperfect tandem repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 534SH2-bindingSequence Analysis
    Motifi374 – 3774SH2-bindingSequence Analysis
    Motifi392 – 40110SH3-bindingSequence Analysis
    Motifi413 – 4164SH2-bindingSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi95 – 995Poly-Pro
    Compositional biasi175 – 418244Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SS18 family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG274014.
    HOVERGENiHBG003892.
    InParanoidiQ15532.
    KOiK15623.
    OMAiNQLIQCI.
    OrthoDBiEOG7B31R1.
    PhylomeDBiQ15532.
    TreeFamiTF330999.

    Family and domain databases

    InterProiIPR007726. SS18_fam.
    [Graphical view]
    PANTHERiPTHR23107. PTHR23107. 1 hit.
    PfamiPF05030. SSXT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q15532-1) [UniParc]FASTAAdd to Basket

    Also known as: SYTins, SYT-L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVAFAAPRQ RGKGEITPAA IQKMLDDNNH LIQCIMDSQN KGKTSECSQY    50
    QQMLHTNLVY LATIADSNQN MQSLLPAPPT QNMPMGPGGM NQSGPPPPPR 100
    SHNMPSDGMV GGGPPAPHMQ NQMNGQMPGP NHMPMQGPGP NQLNMTNSSM 150
    NMPSSSHGSM GGYNHSVPSS QSMPVQNQMT MSQGQPMGNY GPRPNMSMQP 200
    NQGPMMHQQP PSQQYNMPQG GGQHYQGQQP PMGMMGQVNQ GNHMMGQRQI 250
    PPYRPPQQGP PQQYSGQEDY YGDQYSHGGQ GPPEGMNQQY YPDGHNDYGY 300
    QQPSYPEQGY DRPYEDSSQH YYEGGNSQYG QQQDAYQGPP PQQGYPPQQQ 350
    QYPGQQGYPG QQQGYGPSQG GPGPQYPNYP QGQGQQYGGY RPTQPGPPQP 400
    PQQRPYGYDQ GQYGNYQQ 418
    Length:418
    Mass (Da):45,929
    Last modified:January 31, 2002 - v3
    Checksum:i7E089D557538252F
    GO
    Isoform 2 (identifier: Q15532-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         295-325: Missing.

    Show »
    Length:387
    Mass (Da):42,216
    Checksum:iA1D64C2C588C532A
    GO

    Sequence cautioni

    The sequence AAK21314.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA55792.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei295 – 32531Missing in isoform 2. 1 PublicationVSP_006258Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79201 mRNA. Translation: CAA55792.1. Different initiation.
    X79200 mRNA. No translation available.
    AF343880 mRNA. Translation: AAK21314.1. Different initiation.
    EF445031 Genomic DNA. Translation: ACA06073.1.
    CH471088 Genomic DNA. Translation: EAX01210.1.
    BC096223 mRNA. Translation: AAH96223.1.
    S79894 mRNA. Translation: AAB35674.1. Different termination.
    CCDSiCCDS32807.1. [Q15532-1]
    CCDS54183.1. [Q15532-2]
    PIRiS46269.
    RefSeqiNP_001007560.1. NM_001007559.1. [Q15532-1]
    NP_005628.2. NM_005637.2. [Q15532-2]
    UniGeneiHs.129261.

    Genome annotation databases

    GeneIDi6760.
    KEGGihsa:6760.
    UCSCiuc002kvm.3. human. [Q15532-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Chromosomal rearrangement

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79201 mRNA. Translation: CAA55792.1 . Different initiation.
    X79200 mRNA. No translation available.
    AF343880 mRNA. Translation: AAK21314.1 . Different initiation.
    EF445031 Genomic DNA. Translation: ACA06073.1 .
    CH471088 Genomic DNA. Translation: EAX01210.1 .
    BC096223 mRNA. Translation: AAH96223.1 .
    S79894 mRNA. Translation: AAB35674.1 . Different termination.
    CCDSi CCDS32807.1. [Q15532-1 ]
    CCDS54183.1. [Q15532-2 ]
    PIRi S46269.
    RefSeqi NP_001007560.1. NM_001007559.1. [Q15532-1 ]
    NP_005628.2. NM_005637.2. [Q15532-2 ]
    UniGenei Hs.129261.

    3D structure databases

    ProteinModelPortali Q15532.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112638. 21 interactions.
    IntActi Q15532. 16 interactions.
    STRINGi 9606.ENSP00000269138.

    PTM databases

    PhosphoSitei Q15532.

    Proteomic databases

    MaxQBi Q15532.
    PaxDbi Q15532.
    PRIDEi Q15532.

    Protocols and materials databases

    DNASUi 6760.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6760.
    KEGGi hsa:6760.
    UCSCi uc002kvm.3. human. [Q15532-1 ]

    Organism-specific databases

    CTDi 6760.
    GeneCardsi GC18M023596.
    HGNCi HGNC:11340. SS18.
    HPAi HPA055741.
    HPA059539.
    MIMi 600192. gene.
    neXtProti NX_Q15532.
    Orphaneti 3273. Synovial sarcoma.
    PharmGKBi PA36164.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG274014.
    HOVERGENi HBG003892.
    InParanoidi Q15532.
    KOi K15623.
    OMAi NQLIQCI.
    OrthoDBi EOG7B31R1.
    PhylomeDBi Q15532.
    TreeFami TF330999.

    Miscellaneous databases

    ChiTaRSi SS18. human.
    GeneWikii SS18.
    GenomeRNAii 6760.
    NextBioi 26388.
    PROi Q15532.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15532.
    Bgeei Q15532.
    CleanExi HS_SS18.
    Genevestigatori Q15532.

    Family and domain databases

    InterProi IPR007726. SS18_fam.
    [Graphical view ]
    PANTHERi PTHR23107. PTHR23107. 1 hit.
    Pfami PF05030. SSXT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma."
      Clark J., Rocques P.J., Crew A.J., Gill S., Shipley J., Chan A.M.-L., Gusterson B.A., Cooper C.S.
      Nat. Genet. 7:502-508(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SSXT-SSX2 FUSION PROTEIN.
      Tissue: Synovial sarcoma.
    2. "Cloning and characterization of spliced fusion transcript variants of synovial sarcoma: SYT/SSX4, SYT/SSX4v, and SYT/SSX2v. Possible regulatory role of the fusion gene product in wild type SYT expression."
      Brodin B., Haslam K., Yang K., Bartolazzi A., Xie Y., Starborg M., Lundeberg J., Larsson O.
      Gene 268:173-182(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. NHLBI resequencing and genotyping service (RS&G)
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript."
      Fligman I., Lonardo F., Jhanwar S.C., Gerald W.L., Woodruff J., Ladanyi M.
      Am. J. Pathol. 147:1592-1599(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-410 (SSXT-SSX2 FUSION PROTEIN).
    7. "The synovial sarcoma associated protein SYT interacts with the acute leukemia associated protein AF10."
      de Bruijn D.R., dos Santos N.R., Thijssen J., Balemans M., Debernardi S., Linder B., Young B.D., Geurts van Kessel A.
      Oncogene 20:3281-3289(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MLLT10.
    8. "Synovial sarcoma translocation (SYT) encodes a nuclear receptor coactivator."
      Iwasaki T., Koibuchi N., Chin W.W.
      Endocrinology 146:3892-3899(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ALTERNATIVE SPLICING, INTERACTION WITH RBM14.
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSSXT_HUMAN
    AccessioniPrimary (citable) accession number: Q15532
    Secondary accession number(s): B0YJ95
    , Q16404, Q4VAX1, Q9BXC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3