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Q15532 (SSXT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SSXT
Alternative name(s):
Protein SYT
Synovial sarcoma translocated to X chromosome protein
Gene names
Name:SS18
Synonyms:SSXT, SYT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation. Ref.8

Subunit structure

Interacts with MLLT10. Isoform 1 interacts with RBM14 isoform 1. Isoform 2 interacts with RBM14 isoform 1. Ref.7 Ref.8

Subcellular location

Nucleus Probable.

Tissue specificity

Fairly ubiquitously expressed. Expressed in synovial sarcomas and in other human cell lines. The fusion genes SSXT-SSX1 and SSXT-SSX2 are expressed only in synovial sarcomas.

Involvement in disease

A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).

Sequence similarities

Belongs to the SS18 family.

Sequence caution

The sequence AAK21314.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA55792.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATF2P153362EBI-2560599,EBI-1170906

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15532-1)

Also known as: SYTins; SYT-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15532-2)

The sequence of this isoform differs from the canonical sequence as follows:
     295-325: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 418417Protein SSXT
PRO_0000181823

Regions

Repeat344 – 356131
Repeat357 – 369132
Region2 – 186185Transcriptional activation
Region344 – 369262 X 13 AA imperfect tandem repeats
Motif50 – 534SH2-binding Potential
Motif374 – 3774SH2-binding Potential
Motif392 – 40110SH3-binding Potential
Motif413 – 4164SH2-binding Potential
Compositional bias95 – 995Poly-Pro
Compositional bias175 – 418244Gln-rich

Sites

Site366 – 3672Breakpoint for translocation to form the SSXT-SSX1 fusion protein (rare)
Site410 – 4112Breakpoint for translocation to form the SSXT-SSX1 or SSXT-SSX2 fusion proteins

Amino acid modifications

Modified residue21N-acetylserine Ref.9

Natural variations

Alternative sequence295 – 32531Missing in isoform 2.
VSP_006258

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SYTins) (SYT-L) [UniParc].

Last modified January 31, 2002. Version 3.
Checksum: 7E089D557538252F

FASTA41845,929
        10         20         30         40         50         60 
MSVAFAAPRQ RGKGEITPAA IQKMLDDNNH LIQCIMDSQN KGKTSECSQY QQMLHTNLVY 

        70         80         90        100        110        120 
LATIADSNQN MQSLLPAPPT QNMPMGPGGM NQSGPPPPPR SHNMPSDGMV GGGPPAPHMQ 

       130        140        150        160        170        180 
NQMNGQMPGP NHMPMQGPGP NQLNMTNSSM NMPSSSHGSM GGYNHSVPSS QSMPVQNQMT 

       190        200        210        220        230        240 
MSQGQPMGNY GPRPNMSMQP NQGPMMHQQP PSQQYNMPQG GGQHYQGQQP PMGMMGQVNQ 

       250        260        270        280        290        300 
GNHMMGQRQI PPYRPPQQGP PQQYSGQEDY YGDQYSHGGQ GPPEGMNQQY YPDGHNDYGY 

       310        320        330        340        350        360 
QQPSYPEQGY DRPYEDSSQH YYEGGNSQYG QQQDAYQGPP PQQGYPPQQQ QYPGQQGYPG 

       370        380        390        400        410 
QQQGYGPSQG GPGPQYPNYP QGQGQQYGGY RPTQPGPPQP PQQRPYGYDQ GQYGNYQQ 

« Hide

Isoform 2 [UniParc].

Checksum: A1D64C2C588C532A
Show »

FASTA38742,216

References

« Hide 'large scale' references
[1]"Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma."
Clark J., Rocques P.J., Crew A.J., Gill S., Shipley J., Chan A.M.-L., Gusterson B.A., Cooper C.S.
Nat. Genet. 7:502-508(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SSXT-SSX2 FUSION PROTEIN.
Tissue: Synovial sarcoma.
[2]"Cloning and characterization of spliced fusion transcript variants of synovial sarcoma: SYT/SSX4, SYT/SSX4v, and SYT/SSX2v. Possible regulatory role of the fusion gene product in wild type SYT expression."
Brodin B., Haslam K., Yang K., Bartolazzi A., Xie Y., Starborg M., Lundeberg J., Larsson O.
Gene 268:173-182(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]NHLBI resequencing and genotyping service (RS&G)
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Molecular diagnosis of synovial sarcoma and characterization of a variant SYT-SSX2 fusion transcript."
Fligman I., Lonardo F., Jhanwar S.C., Gerald W.L., Woodruff J., Ladanyi M.
Am. J. Pathol. 147:1592-1599(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-410 (SSXT-SSX2 FUSION PROTEIN).
[7]"The synovial sarcoma associated protein SYT interacts with the acute leukemia associated protein AF10."
de Bruijn D.R., dos Santos N.R., Thijssen J., Balemans M., Debernardi S., Linder B., Young B.D., Geurts van Kessel A.
Oncogene 20:3281-3289(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MLLT10.
[8]"Synovial sarcoma translocation (SYT) encodes a nuclear receptor coactivator."
Iwasaki T., Koibuchi N., Chin W.W.
Endocrinology 146:3892-3899(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ALTERNATIVE SPLICING, INTERACTION WITH RBM14.
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79201 mRNA. Translation: CAA55792.1. Different initiation.
X79200 mRNA. No translation available.
AF343880 mRNA. Translation: AAK21314.1. Different initiation.
EF445031 Genomic DNA. Translation: ACA06073.1.
CH471088 Genomic DNA. Translation: EAX01210.1.
BC096223 mRNA. Translation: AAH96223.1.
S79894 mRNA. Translation: AAB35674.1. Different termination.
PIRS46269.
RefSeqNP_001007560.1. NM_001007559.1.
NP_005628.2. NM_005637.2.
UniGeneHs.129261.

3D structure databases

ProteinModelPortalQ15532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112638. 21 interactions.
IntActQ15532. 16 interactions.
STRING9606.ENSP00000269138.

PTM databases

PhosphoSiteQ15532.

Proteomic databases

PaxDbQ15532.
PRIDEQ15532.

Protocols and materials databases

DNASU6760.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000269137; ENSP00000269137; ENSG00000141380. [Q15532-2]
ENST00000415083; ENSP00000414516; ENSG00000141380. [Q15532-1]
GeneID6760.
KEGGhsa:6760.
UCSCuc002kvm.3. human. [Q15532-1]

Organism-specific databases

CTD6760.
GeneCardsGC18M023596.
HGNCHGNC:11340. SS18.
HPAHPA055741.
HPA059539.
MIM600192. gene.
neXtProtNX_Q15532.
Orphanet3273. Synovial sarcoma.
PharmGKBPA36164.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG274014.
HOVERGENHBG003892.
InParanoidQ15532.
KOK15623.
OMANQLIQCI.
OrthoDBEOG7B31R1.
PhylomeDBQ15532.
TreeFamTF330999.

Gene expression databases

ArrayExpressQ15532.
BgeeQ15532.
CleanExHS_SS18.
GenevestigatorQ15532.

Family and domain databases

InterProIPR007726. SS18_fam.
[Graphical view]
PANTHERPTHR23107. PTHR23107. 1 hit.
PfamPF05030. SSXT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSS18. human.
GeneWikiSS18.
GenomeRNAi6760.
NextBio26388.
PROQ15532.
SOURCESearch...

Entry information

Entry nameSSXT_HUMAN
AccessionPrimary (citable) accession number: Q15532
Secondary accession number(s): B0YJ95 expand/collapse secondary AC list , Q16404, Q4VAX1, Q9BXC6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 31, 2002
Last modified: April 16, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM