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Q15528 (MED22_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name=Surf-5
Gene names
Name:MED22
Synonyms:SURF5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Ref.8

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 22 family.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Traceable author statement Ref.1. Source: ProtInc

mediator complex

Inferred from direct assay PubMed 14638676. Source: MGI

   Molecular_functionRNA polymerase II transcription cofactor activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction Ref.7. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MED11Q9P0863EBI-394687,EBI-394704
Med30Q9CQI94EBI-394687,EBI-309220From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Surf5B (identifier: Q15528-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Surf5A (identifier: Q15528-2)

The sequence of this isoform differs from the canonical sequence as follows:
     138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Mediator of RNA polymerase II transcription subunit 22
PRO_0000178838

Regions

Coiled coil93 – 12230 Potential
Compositional bias136 – 1405Poly-Ser

Natural variations

Alternative sequence138 – 20063SSSLC…PTEHA → RYK in isoform Surf5A.
VSP_006309

Sequences

Sequence LengthMass (Da)Tools
Isoform Surf5B [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 113BBF7C35B03F7B

FASTA20022,221
        10         20         30         40         50         60 
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE 

        70         80         90        100        110        120 
MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRTLQE ECDRKLITLR 

       130        140        150        160        170        180 
DEISIDLYEL EEEYYSSSSS LCEANDLPLC EAYGRLDLDT DSADGLSAPL LASPEPSAGP 

       190        200 
LQVAAPAHSH AGGPGPTEHA 

« Hide

Isoform Surf5A [UniParc].

Checksum: 8D720D36F1171AE1
Show »

FASTA14016,480

References

« Hide 'large scale' references
[1]"Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene."
Garson K., Duhig T., Armes N., Colombo P., Fried M.
Genomics 30:163-170(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SURF5A).
[2]"The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts."
Angiolillo A., Russo G., Porcellini A., Smaldone S., D'Alessandro F., Pietropaolo C.
Gene 284:169-178(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS SURF5B AND SURF5A).
Tissue: Cerebellum and Testis.
[4]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SURF5A).
Tissue: Muscle and Skin.
[7]"Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED10; MED11 AND MED30.
[8]"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X85178 mRNA. Translation: CAA59462.1.
AJ224639 Genomic DNA. Translation: CAA12052.1.
AJ224639 Genomic DNA. Translation: CAA12053.1.
AJ224358 mRNA. Translation: CAA11915.1.
AJ224359 mRNA. Translation: CAA11916.1.
AK124518 mRNA. Translation: BAG54045.1.
AK126069 mRNA. Translation: BAG54286.1.
AL158826 Genomic DNA. Translation: CAI12827.1.
CH471090 Genomic DNA. Translation: EAW88056.1.
CH471090 Genomic DNA. Translation: EAW88060.1.
BC024225 mRNA. Translation: AAH24225.1.
BC040111 mRNA. Translation: AAH40111.1.
CCDSCCDS6963.1. [Q15528-1]
CCDS6964.1. [Q15528-2]
RefSeqNP_598395.1. NM_133640.4. [Q15528-1]
NP_852468.1. NM_181491.2. [Q15528-2]
UniGeneHs.78354.

3D structure databases

ProteinModelPortalQ15528.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112704. 26 interactions.
IntActQ15528. 13 interactions.
MINTMINT-1397525.
STRING9606.ENSP00000342343.

PTM databases

PhosphoSiteQ15528.

Polymorphism databases

DMDM6175074.

Proteomic databases

MaxQBQ15528.
PaxDbQ15528.
PRIDEQ15528.

Protocols and materials databases

DNASU6837.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000343730; ENSP00000342343; ENSG00000148297. [Q15528-1]
ENST00000344469; ENSP00000341761; ENSG00000148297. [Q15528-2]
ENST00000476080; ENSP00000419098; ENSG00000148297. [Q15528-2]
ENST00000491289; ENSP00000420393; ENSG00000148297. [Q15528-1]
ENST00000564120; ENSP00000456245; ENSG00000261579. [Q15528-2]
ENST00000565731; ENSP00000454651; ENSG00000261579. [Q15528-1]
ENST00000566494; ENSP00000456763; ENSG00000261579. [Q15528-2]
ENST00000567581; ENSP00000454853; ENSG00000261579. [Q15528-1]
ENST00000570094; ENSP00000456911; ENSG00000261579. [Q15528-2]
GeneID6837.
KEGGhsa:6837.
UCSCuc004cdc.3. human. [Q15528-1]
uc004cdd.3. human. [Q15528-2]

Organism-specific databases

CTD6837.
GeneCardsGC09M136205.
HGNCHGNC:11477. MED22.
HPAHPA020391.
MIM185641. gene.
neXtProtNX_Q15528.
PharmGKBPA162395486.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG271715.
HOGENOMHOG000006377.
HOVERGENHBG058487.
InParanoidQ15528.
KOK15139.
OMAEQDHYEM.
PhylomeDBQ15528.
TreeFamTF323390.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.

Gene expression databases

ArrayExpressQ15528.
BgeeQ15528.
CleanExHS_MED22.
GenevestigatorQ15528.

Family and domain databases

InterProIPR009332. Mediator_Med22.
[Graphical view]
PANTHERPTHR12434. PTHR12434. 1 hit.
PfamPF06179. Med22. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiMED22.
GenomeRNAi6837.
NextBio26691.
PROQ15528.
SOURCESearch...

Entry information

Entry nameMED22_HUMAN
AccessionPrimary (citable) accession number: Q15528
Secondary accession number(s): B3KW83 expand/collapse secondary AC list , B3KWX4, O76072, Q5T8U0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM