Q15528 (MED22_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mediator of RNA polymerase II transcription subunit 22

Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name=Surf-5

Gene names

Name:	MED22
Synonyms:	SURF5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subunit structure	Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Ref.8
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the Mediator complex subunit 22 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Traceable author statement Ref.1. Source: ProtInc mediator complex Inferred from direct assay PubMed 14638676. Source: MGI
Molecular_function	RNA polymerase II transcription cofactor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MED11	Q9P086	3	EBI-394687,EBI-394704
Med30	Q9CQI9	4	EBI-394687,EBI-309220	From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Surf5B (identifier: Q15528-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Surf5A (identifier: Q15528-2)

The sequence of this isoform differs from the canonical sequence as follows:
     138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Mediator of RNA polymerase II transcription subunit 22		PRO_0000178838
Regions
	Coiled coil	93 – 122	30	Potential
	Compositional bias	136 – 140	5	Poly-Ser
Natural variations
	Alternative sequence	138 – 200	63	SSSLC…PTEHA → RYK in isoform Surf5A.		VSP_006309

Sequences

	Sequence		Length	Mass (Da)
	Isoform Surf5B [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 113BBF7C35B03F7B	FASTA	200	22,221
	10 20 30 40 50 60 MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE 70 80 90 100 110 120 MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRTLQE ECDRKLITLR 130 140 150 160 170 180 DEISIDLYEL EEEYYSSSSS LCEANDLPLC EAYGRLDLDT DSADGLSAPL LASPEPSAGP 190 200 LQVAAPAHSH AGGPGPTEHA
	Isoform Surf5A [UniParc]. Checksum: 8D720D36F1171AE1	FASTA	140	16,480
	10 20 30 40 50 60 MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE 70 80 90 100 110 120 MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRTLQE ECDRKLITLR 130 140 DEISIDLYEL EEEYYSSRYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene." Garson K., Duhig T., Armes N., Colombo P., Fried M. Genomics 30:163-170(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SURF5A).
[2]	"The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts." Angiolillo A., Russo G., Porcellini A., Smaldone S., D'Alessandro F., Pietropaolo C. Gene 284:169-178(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS SURF5B AND SURF5A). Tissue: Cerebellum and Testis.
[4]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SURF5A). Tissue: Muscle and Skin.
[7]	"Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3." Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W. J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MED10; MED11 AND MED30.
[8]	"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription." Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G. Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X85178 mRNA. Translation: CAA59462.1. AJ224639 Genomic DNA. Translation: CAA12052.1. AJ224639 Genomic DNA. Translation: CAA12053.1. AJ224358 mRNA. Translation: CAA11915.1. AJ224359 mRNA. Translation: CAA11916.1. AK124518 mRNA. Translation: BAG54045.1. AK126069 mRNA. Translation: BAG54286.1. AL158826 Genomic DNA. Translation: CAI12827.1. CH471090 Genomic DNA. Translation: EAW88056.1. CH471090 Genomic DNA. Translation: EAW88060.1. BC024225 mRNA. Translation: AAH24225.1. BC040111 mRNA. Translation: AAH40111.1.
IPI	IPI00018040. IPI00219228.
RefSeq	NP_598395.1. NM_133640.3. NP_852468.1. NM_181491.1.
UniGene	Hs.78354.
3D structure databases
ProteinModelPortal	Q15528.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15528. 12 interactions.
MINT	MINT-1397525.
STRING	9606.ENSP00000342343.
PTM databases
PhosphoSite	Q15528.
Polymorphism databases
DMDM	6175074.
Proteomic databases
PaxDb	Q15528.
PRIDE	Q15528.
Protocols and materials databases
DNASU	6837.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000343730; ENSP00000342343; ENSG00000148297. ENST00000344469; ENSP00000341761; ENSG00000148297. ENST00000476080; ENSP00000419098; ENSG00000148297. ENST00000486395; ENSP00000420276; ENSG00000148297. ENST00000491289; ENSP00000420393; ENSG00000148297. ENST00000564120; ENSP00000456245; ENSG00000261579. ENST00000565731; ENSP00000454651; ENSG00000261579. ENST00000566494; ENSP00000456763; ENSG00000261579. ENST00000567581; ENSP00000454853; ENSG00000261579. ENST00000570094; ENSP00000456911; ENSG00000261579.
GeneID	6837.
KEGG	hsa:6837.
UCSC	uc004cdc.3. human. uc004cdd.3. human.
Organism-specific databases
CTD	6837.
GeneCards	GC09M136205.
HGNC	HGNC:11477. MED22.
HPA	HPA020391.
MIM	185641. gene.
neXtProt	NX_Q15528.
PharmGKB	PA162395486.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271715.
HOGENOM	HOG000006377.
HOVERGEN	HBG058487.
InParanoid	Q15528.
KO	K15139.
OMA	VKSIMDN.
OrthoDB	EOG4X3H2K.
PhylomeDB	Q15528.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology.
Gene expression databases
ArrayExpress	Q15528.
Bgee	Q15528.
CleanEx	HS_MED22.
Genevestigator	Q15528.
GermOnline	ENSG00000148297. Homo sapiens.
Family and domain databases
InterPro	IPR009332. Mediator_Med22. [Graphical view]
PANTHER	PTHR12434. PTHR12434. 1 hit.
Pfam	PF06179. Med22. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6837.
NextBio	26691.
SOURCE	Search...

Entry information

Entry name

MED22_HUMAN

Accession

Primary (citable) accession number: Q15528
Secondary accession number(s): B3KW83 , B3KWX4, O76072, Q5T8U0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.