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Q15528

- MED22_HUMAN

UniProt

Q15528 - MED22_HUMAN

Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

MED22

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA polymerase II transcription cofactor activity Source: InterPro

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mediator of RNA polymerase II transcription subunit 22
    Alternative name(s):
    Mediator complex subunit 22
    Surfeit locus protein 5
    Short name:
    Surf-5
    Gene namesi
    Name:MED22
    Synonyms:SURF5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 9

    Organism-specific databases

    HGNCiHGNC:11477. MED22.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. cytoplasm Source: ProtInc
    2. mediator complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395486.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Mediator of RNA polymerase II transcription subunit 22PRO_0000178838Add
    BLAST

    Proteomic databases

    MaxQBiQ15528.
    PaxDbiQ15528.
    PRIDEiQ15528.

    PTM databases

    PhosphoSiteiQ15528.

    Expressioni

    Gene expression databases

    ArrayExpressiQ15528.
    BgeeiQ15528.
    CleanExiHS_MED22.
    GenevestigatoriQ15528.

    Organism-specific databases

    HPAiHPA020391.

    Interactioni

    Subunit structurei

    Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MED11Q9P0863EBI-394687,EBI-394704
    Med30Q9CQI94EBI-394687,EBI-309220From a different organism.

    Protein-protein interaction databases

    BioGridi112704. 26 interactions.
    IntActiQ15528. 13 interactions.
    MINTiMINT-1397525.
    STRINGi9606.ENSP00000342343.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15528.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili93 – 12230Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi136 – 1405Poly-Ser

    Sequence similaritiesi

    Belongs to the Mediator complex subunit 22 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG271715.
    HOGENOMiHOG000006377.
    HOVERGENiHBG058487.
    InParanoidiQ15528.
    KOiK15139.
    OMAiEQDHYEM.
    PhylomeDBiQ15528.
    TreeFamiTF323390.

    Family and domain databases

    InterProiIPR009332. Mediator_Med22.
    [Graphical view]
    PANTHERiPTHR12434. PTHR12434. 1 hit.
    PfamiPF06179. Med22. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Surf5B (identifier: Q15528-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR    50
    ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ 100
    RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSSSS LCEANDLPLC 150
    EAYGRLDLDT DSADGLSAPL LASPEPSAGP LQVAAPAHSH AGGPGPTEHA 200
    Length:200
    Mass (Da):22,221
    Last modified:July 15, 1999 - v2
    Checksum:i113BBF7C35B03F7B
    GO
    Isoform Surf5A (identifier: Q15528-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK

    Show »
    Length:140
    Mass (Da):16,480
    Checksum:i8D720D36F1171AE1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei138 – 20063SSSLC…PTEHA → RYK in isoform Surf5A. 3 PublicationsVSP_006309Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85178 mRNA. Translation: CAA59462.1.
    AJ224639 Genomic DNA. Translation: CAA12052.1.
    AJ224639 Genomic DNA. Translation: CAA12053.1.
    AJ224358 mRNA. Translation: CAA11915.1.
    AJ224359 mRNA. Translation: CAA11916.1.
    AK124518 mRNA. Translation: BAG54045.1.
    AK126069 mRNA. Translation: BAG54286.1.
    AL158826 Genomic DNA. Translation: CAI12827.1.
    CH471090 Genomic DNA. Translation: EAW88056.1.
    CH471090 Genomic DNA. Translation: EAW88060.1.
    BC024225 mRNA. Translation: AAH24225.1.
    BC040111 mRNA. Translation: AAH40111.1.
    CCDSiCCDS6963.1. [Q15528-1]
    CCDS6964.1. [Q15528-2]
    RefSeqiNP_598395.1. NM_133640.4. [Q15528-1]
    NP_852468.1. NM_181491.2. [Q15528-2]
    UniGeneiHs.78354.

    Genome annotation databases

    EnsembliENST00000343730; ENSP00000342343; ENSG00000148297. [Q15528-1]
    GeneIDi6837.
    KEGGihsa:6837.
    UCSCiuc004cdc.3. human. [Q15528-1]
    uc004cdd.3. human. [Q15528-2]

    Polymorphism databases

    DMDMi6175074.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X85178 mRNA. Translation: CAA59462.1 .
    AJ224639 Genomic DNA. Translation: CAA12052.1 .
    AJ224639 Genomic DNA. Translation: CAA12053.1 .
    AJ224358 mRNA. Translation: CAA11915.1 .
    AJ224359 mRNA. Translation: CAA11916.1 .
    AK124518 mRNA. Translation: BAG54045.1 .
    AK126069 mRNA. Translation: BAG54286.1 .
    AL158826 Genomic DNA. Translation: CAI12827.1 .
    CH471090 Genomic DNA. Translation: EAW88056.1 .
    CH471090 Genomic DNA. Translation: EAW88060.1 .
    BC024225 mRNA. Translation: AAH24225.1 .
    BC040111 mRNA. Translation: AAH40111.1 .
    CCDSi CCDS6963.1. [Q15528-1 ]
    CCDS6964.1. [Q15528-2 ]
    RefSeqi NP_598395.1. NM_133640.4. [Q15528-1 ]
    NP_852468.1. NM_181491.2. [Q15528-2 ]
    UniGenei Hs.78354.

    3D structure databases

    ProteinModelPortali Q15528.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112704. 26 interactions.
    IntActi Q15528. 13 interactions.
    MINTi MINT-1397525.
    STRINGi 9606.ENSP00000342343.

    PTM databases

    PhosphoSitei Q15528.

    Polymorphism databases

    DMDMi 6175074.

    Proteomic databases

    MaxQBi Q15528.
    PaxDbi Q15528.
    PRIDEi Q15528.

    Protocols and materials databases

    DNASUi 6837.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000343730 ; ENSP00000342343 ; ENSG00000148297 . [Q15528-1 ]
    GeneIDi 6837.
    KEGGi hsa:6837.
    UCSCi uc004cdc.3. human. [Q15528-1 ]
    uc004cdd.3. human. [Q15528-2 ]

    Organism-specific databases

    CTDi 6837.
    GeneCardsi GC09M136205.
    HGNCi HGNC:11477. MED22.
    HPAi HPA020391.
    MIMi 185641. gene.
    neXtProti NX_Q15528.
    PharmGKBi PA162395486.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG271715.
    HOGENOMi HOG000006377.
    HOVERGENi HBG058487.
    InParanoidi Q15528.
    KOi K15139.
    OMAi EQDHYEM.
    PhylomeDBi Q15528.
    TreeFami TF323390.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_27161. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    GeneWikii MED22.
    GenomeRNAii 6837.
    NextBioi 26691.
    PROi Q15528.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15528.
    Bgeei Q15528.
    CleanExi HS_MED22.
    Genevestigatori Q15528.

    Family and domain databases

    InterProi IPR009332. Mediator_Med22.
    [Graphical view ]
    PANTHERi PTHR12434. PTHR12434. 1 hit.
    Pfami PF06179. Med22. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene."
      Garson K., Duhig T., Armes N., Colombo P., Fried M.
      Genomics 30:163-170(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SURF5A).
    2. "The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts."
      Angiolillo A., Russo G., Porcellini A., Smaldone S., D'Alessandro F., Pietropaolo C.
      Gene 284:169-178(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS SURF5B AND SURF5A).
      Tissue: Cerebellum and Testis.
    4. "DNA sequence and analysis of human chromosome 9."
      Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
      , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
      Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SURF5A).
      Tissue: Muscle and Skin.
    7. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
      Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
      J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED10; MED11 AND MED30.
    8. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
      Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
      Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMED22_HUMAN
    AccessioniPrimary (citable) accession number: Q15528
    Secondary accession number(s): B3KW83
    , B3KWX4, O76072, Q5T8U0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 9
      Human chromosome 9: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3