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Q15528

- MED22_HUMAN

UniProt

Q15528 - MED22_HUMAN

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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

MED22

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name:
Surf-5
Gene namesi
Name:MED22
Synonyms:SURF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 9

Organism-specific databases

HGNCiHGNC:11477. MED22.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: ProtInc
  2. mediator complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162395486.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Mediator of RNA polymerase II transcription subunit 22PRO_0000178838Add
BLAST

Proteomic databases

MaxQBiQ15528.
PaxDbiQ15528.
PRIDEiQ15528.

PTM databases

PhosphoSiteiQ15528.

Expressioni

Gene expression databases

BgeeiQ15528.
CleanExiHS_MED22.
ExpressionAtlasiQ15528. baseline and differential.
GenevestigatoriQ15528.

Organism-specific databases

HPAiHPA020391.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MED11Q9P0863EBI-394687,EBI-394704
Med30Q9CQI94EBI-394687,EBI-309220From a different organism.

Protein-protein interaction databases

BioGridi112704. 28 interactions.
IntActiQ15528. 13 interactions.
MINTiMINT-1397525.
STRINGi9606.ENSP00000342343.

Structurei

3D structure databases

ProteinModelPortaliQ15528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili93 – 12230Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi136 – 1405Poly-Ser

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG271715.
GeneTreeiENSGT00390000004339.
HOGENOMiHOG000006377.
HOVERGENiHBG058487.
InParanoidiQ15528.
KOiK15139.
OMAiEQDHYEM.
PhylomeDBiQ15528.
TreeFamiTF323390.

Family and domain databases

InterProiIPR009332. Mediator_Med22.
[Graphical view]
PANTHERiPTHR12434. PTHR12434. 1 hit.
PfamiPF06179. Med22. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Surf5B (identifier: Q15528-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYGRLDLDT DSADGLSAPL LASPEPSAGP LQVAAPAHSH AGGPGPTEHA
Length:200
Mass (Da):22,221
Last modified:July 15, 1999 - v2
Checksum:i113BBF7C35B03F7B
GO
Isoform Surf5A (identifier: Q15528-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK

Show »
Length:140
Mass (Da):16,480
Checksum:i8D720D36F1171AE1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei138 – 20063SSSLC…PTEHA → RYK in isoform Surf5A. 3 PublicationsVSP_006309Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85178 mRNA. Translation: CAA59462.1.
AJ224639 Genomic DNA. Translation: CAA12052.1.
AJ224639 Genomic DNA. Translation: CAA12053.1.
AJ224358 mRNA. Translation: CAA11915.1.
AJ224359 mRNA. Translation: CAA11916.1.
AK124518 mRNA. Translation: BAG54045.1.
AK126069 mRNA. Translation: BAG54286.1.
AL158826 Genomic DNA. Translation: CAI12827.1.
CH471090 Genomic DNA. Translation: EAW88056.1.
CH471090 Genomic DNA. Translation: EAW88060.1.
BC024225 mRNA. Translation: AAH24225.1.
BC040111 mRNA. Translation: AAH40111.1.
CCDSiCCDS6963.1. [Q15528-1]
CCDS6964.1. [Q15528-2]
RefSeqiNP_598395.1. NM_133640.4. [Q15528-1]
NP_852468.1. NM_181491.2. [Q15528-2]
UniGeneiHs.78354.

Genome annotation databases

EnsembliENST00000343730; ENSP00000342343; ENSG00000148297. [Q15528-1]
ENST00000610672; ENSP00000482438; ENSG00000148297. [Q15528-1]
ENST00000610888; ENSP00000478773; ENSG00000148297. [Q15528-2]
ENST00000614493; ENSP00000481493; ENSG00000148297. [Q15528-2]
GeneIDi6837.
KEGGihsa:6837.
UCSCiuc004cdc.3. human. [Q15528-1]
uc004cdd.3. human. [Q15528-2]

Polymorphism databases

DMDMi6175074.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85178 mRNA. Translation: CAA59462.1 .
AJ224639 Genomic DNA. Translation: CAA12052.1 .
AJ224639 Genomic DNA. Translation: CAA12053.1 .
AJ224358 mRNA. Translation: CAA11915.1 .
AJ224359 mRNA. Translation: CAA11916.1 .
AK124518 mRNA. Translation: BAG54045.1 .
AK126069 mRNA. Translation: BAG54286.1 .
AL158826 Genomic DNA. Translation: CAI12827.1 .
CH471090 Genomic DNA. Translation: EAW88056.1 .
CH471090 Genomic DNA. Translation: EAW88060.1 .
BC024225 mRNA. Translation: AAH24225.1 .
BC040111 mRNA. Translation: AAH40111.1 .
CCDSi CCDS6963.1. [Q15528-1 ]
CCDS6964.1. [Q15528-2 ]
RefSeqi NP_598395.1. NM_133640.4. [Q15528-1 ]
NP_852468.1. NM_181491.2. [Q15528-2 ]
UniGenei Hs.78354.

3D structure databases

ProteinModelPortali Q15528.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 112704. 28 interactions.
IntActi Q15528. 13 interactions.
MINTi MINT-1397525.
STRINGi 9606.ENSP00000342343.

PTM databases

PhosphoSitei Q15528.

Polymorphism databases

DMDMi 6175074.

Proteomic databases

MaxQBi Q15528.
PaxDbi Q15528.
PRIDEi Q15528.

Protocols and materials databases

DNASUi 6837.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000343730 ; ENSP00000342343 ; ENSG00000148297 . [Q15528-1 ]
ENST00000610672 ; ENSP00000482438 ; ENSG00000148297 . [Q15528-1 ]
ENST00000610888 ; ENSP00000478773 ; ENSG00000148297 . [Q15528-2 ]
ENST00000614493 ; ENSP00000481493 ; ENSG00000148297 . [Q15528-2 ]
GeneIDi 6837.
KEGGi hsa:6837.
UCSCi uc004cdc.3. human. [Q15528-1 ]
uc004cdd.3. human. [Q15528-2 ]

Organism-specific databases

CTDi 6837.
GeneCardsi GC09M136205.
HGNCi HGNC:11477. MED22.
HPAi HPA020391.
MIMi 185641. gene.
neXtProti NX_Q15528.
PharmGKBi PA162395486.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG271715.
GeneTreei ENSGT00390000004339.
HOGENOMi HOG000006377.
HOVERGENi HBG058487.
InParanoidi Q15528.
KOi K15139.
OMAi EQDHYEM.
PhylomeDBi Q15528.
TreeFami TF323390.

Enzyme and pathway databases

Reactomei REACT_116145. PPARA activates gene expression.
REACT_27161. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

GeneWikii MED22.
GenomeRNAii 6837.
NextBioi 26691.
PROi Q15528.
SOURCEi Search...

Gene expression databases

Bgeei Q15528.
CleanExi HS_MED22.
ExpressionAtlasi Q15528. baseline and differential.
Genevestigatori Q15528.

Family and domain databases

InterProi IPR009332. Mediator_Med22.
[Graphical view ]
PANTHERi PTHR12434. PTHR12434. 1 hit.
Pfami PF06179. Med22. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene."
    Garson K., Duhig T., Armes N., Colombo P., Fried M.
    Genomics 30:163-170(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SURF5A).
  2. "The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts."
    Angiolillo A., Russo G., Porcellini A., Smaldone S., D'Alessandro F., Pietropaolo C.
    Gene 284:169-178(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS SURF5B AND SURF5A).
    Tissue: Cerebellum and Testis.
  4. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SURF5A).
    Tissue: Muscle and Skin.
  7. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."
    Sato S., Tomomori-Sato C., Banks C.A.S., Sorokina I., Parmely T.J., Kong S.E., Jin J., Cai Y., Lane W.S., Brower C.S., Conaway R.C., Conaway J.W.
    J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED10; MED11 AND MED30.
  8. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."
    Zhang X., Krutchinsky A., Fukuda A., Chen W., Yamamura S., Chait B.T., Roeder R.G.
    Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMED22_HUMAN
AccessioniPrimary (citable) accession number: Q15528
Secondary accession number(s): B3KW83
, B3KWX4, O76072, Q5T8U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3