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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

MED22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781. PPARA activates gene expression.
R-HSA-381340. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name:
Surf-5
Gene namesi
Name:MED22
Synonyms:SURF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:11477. MED22.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • mediator complex Source: MGI

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000148297.
PharmGKBiPA162395486.

Polymorphism and mutation databases

DMDMi6175074.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001788381 – 200Mediator of RNA polymerase II transcription subunit 22Add BLAST200

Proteomic databases

EPDiQ15528.
MaxQBiQ15528.
PaxDbiQ15528.
PeptideAtlasiQ15528.
PRIDEiQ15528.
TopDownProteomicsiQ15528-2. [Q15528-2]

PTM databases

iPTMnetiQ15528.
PhosphoSitePlusiQ15528.

Expressioni

Gene expression databases

BgeeiENSG00000148297.
CleanExiHS_MED22.
ExpressionAtlasiQ15528. baseline and differential.
GenevisibleiQ15528. HS.

Organism-specific databases

HPAiHPA020391.
HPA063694.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi112704. 45 interactors.
IntActiQ15528. 23 interactors.
MINTiMINT-1397525.
STRINGi9606.ENSP00000342343.

Structurei

3D structure databases

ProteinModelPortaliQ15528.
SMRiQ15528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 122Sequence analysisAdd BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi136 – 140Poly-Ser5

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3304. Eukaryota.
ENOG410XS6U. LUCA.
GeneTreeiENSGT00390000004339.
HOGENOMiHOG000006377.
HOVERGENiHBG058487.
InParanoidiQ15528.
KOiK15139.
OMAiKQAECDQ.
OrthoDBiEOG091G0SIM.
PhylomeDBiQ15528.
TreeFamiTF323390.

Family and domain databases

InterProiView protein in InterPro
IPR009332. Med22.
PANTHERiPTHR12434. PTHR12434. 1 hit.
PfamiView protein in Pfam
PF06179. Med22. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Surf5B (identifier: Q15528-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYGRLDLDT DSADGLSAPL LASPEPSAGP LQVAAPAHSH AGGPGPTEHA
Length:200
Mass (Da):22,221
Last modified:July 15, 1999 - v2
Checksum:i113BBF7C35B03F7B
GO
Isoform Surf5A (identifier: Q15528-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK

Show »
Length:140
Mass (Da):16,480
Checksum:i8D720D36F1171AE1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006309138 – 200SSSLC…PTEHA → RYK in isoform Surf5A. 3 PublicationsAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85178 mRNA. Translation: CAA59462.1.
AJ224639 Genomic DNA. Translation: CAA12052.1.
AJ224639 Genomic DNA. Translation: CAA12053.1.
AJ224358 mRNA. Translation: CAA11915.1.
AJ224359 mRNA. Translation: CAA11916.1.
AK124518 mRNA. Translation: BAG54045.1.
AK126069 mRNA. Translation: BAG54286.1.
AL158826 Genomic DNA. Translation: CAI12827.1.
CH471090 Genomic DNA. Translation: EAW88056.1.
CH471090 Genomic DNA. Translation: EAW88060.1.
BC024225 mRNA. Translation: AAH24225.1.
BC040111 mRNA. Translation: AAH40111.1.
CCDSiCCDS6963.1. [Q15528-1]
CCDS6964.1. [Q15528-2]
RefSeqiNP_598395.1. NM_133640.4. [Q15528-1]
NP_852468.1. NM_181491.2. [Q15528-2]
UniGeneiHs.78354.

Genome annotation databases

EnsembliENST00000343730; ENSP00000342343; ENSG00000148297. [Q15528-1]
ENST00000610672; ENSP00000482438; ENSG00000148297. [Q15528-1]
ENST00000610888; ENSP00000478773; ENSG00000148297. [Q15528-2]
ENST00000614493; ENSP00000481493; ENSG00000148297. [Q15528-2]
ENST00000626118; ENSP00000485715; ENSG00000281022. [Q15528-2]
ENST00000629522; ENSP00000485880; ENSG00000281022. [Q15528-1]
ENST00000629975; ENSP00000486722; ENSG00000281022. [Q15528-2]
ENST00000631196; ENSP00000487580; ENSG00000281022. [Q15528-1]
GeneIDi6837.
KEGGihsa:6837.
UCSCiuc004cdc.5. human. [Q15528-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED22_HUMAN
AccessioniPrimary (citable) accession number: Q15528
Secondary accession number(s): B3KW83
, B3KWX4, O76072, Q5T8U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: August 30, 2017
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families