UniProtKB - Q15528 (MED22_HUMAN)
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Protein
Mediator of RNA polymerase II transcription subunit 22
Gene
MED22
Organism
Homo sapiens (Human)
Status
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
GO - Molecular functioni
- RNA polymerase II transcription cofactor activity Source: InterPro
GO - Biological processi
- transcription, DNA-templated Source: UniProtKB-KW
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-HSA-1989781. PPARA activates gene expression. R-HSA-381340. Transcriptional regulation of white adipocyte differentiation. |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 22Alternative name(s): Mediator complex subunit 22 Surfeit locus protein 5 Short name: Surf-5 |
Gene namesi | Name:MED22 Synonyms:SURF5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000148297.15. |
HGNCi | HGNC:11477. MED22. |
MIMi | 185641. gene. |
neXtProti | NX_Q15528. |
Pathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000148297. |
PharmGKBi | PA162395486. |
Polymorphism and mutation databases
DMDMi | 6175074. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000178838 | 1 – 200 | Mediator of RNA polymerase II transcription subunit 22Add BLAST | 200 |
Proteomic databases
EPDi | Q15528. |
MaxQBi | Q15528. |
PaxDbi | Q15528. |
PeptideAtlasi | Q15528. |
PRIDEi | Q15528. |
TopDownProteomicsi | Q15528-2. [Q15528-2] |
PTM databases
iPTMneti | Q15528. |
PhosphoSitePlusi | Q15528. |
Expressioni
Gene expression databases
Bgeei | ENSG00000148297. |
CleanExi | HS_MED22. |
ExpressionAtlasi | Q15528. baseline and differential. |
Genevisiblei | Q15528. HS. |
Organism-specific databases
HPAi | HPA020391. HPA063694. |
Interactioni
Subunit structurei
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.1 Publication
Binary interactionsi
Protein-protein interaction databases
BioGridi | 112704. 45 interactors. |
CORUMi | Q15528. |
IntActi | Q15528. 23 interactors. |
STRINGi | 9606.ENSP00000342343. |
Structurei
3D structure databases
ProteinModelPortali | Q15528. |
SMRi | Q15528. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 93 – 122 | Sequence analysisAdd BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 136 – 140 | Poly-Ser | 5 |
Sequence similaritiesi
Belongs to the Mediator complex subunit 22 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3304. Eukaryota. ENOG410XS6U. LUCA. |
GeneTreei | ENSGT00390000004339. |
HOGENOMi | HOG000006377. |
HOVERGENi | HBG058487. |
InParanoidi | Q15528. |
KOi | K15139. |
OMAi | KQAECDQ. |
OrthoDBi | EOG091G0SIM. |
PhylomeDBi | Q15528. |
TreeFami | TF323390. |
Family and domain databases
Gene3Di | 1.20.58.1600. 1 hit. |
InterProi | View protein in InterPro IPR009332. Med22. IPR038427. MED22_sf. |
PANTHERi | PTHR12434. PTHR12434. 1 hit. |
Pfami | View protein in Pfam PF06179. Med22. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform Surf5B (identifier: Q15528-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRTLQE ECDRKLITLR DEISIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYGRLDLDT DSADGLSAPL LASPEPSAGP LQVAAPAHSH AGGPGPTEHA
Isoform Surf5A (identifier: Q15528-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
138-200: SSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA → RYK
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006309 | 138 – 200 | SSSLC…PTEHA → RYK in isoform Surf5A. 3 PublicationsAdd BLAST | 63 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X85178 mRNA. Translation: CAA59462.1. AJ224639 Genomic DNA. Translation: CAA12052.1. AJ224639 Genomic DNA. Translation: CAA12053.1. AJ224358 mRNA. Translation: CAA11915.1. AJ224359 mRNA. Translation: CAA11916.1. AK124518 mRNA. Translation: BAG54045.1. AK126069 mRNA. Translation: BAG54286.1. AL158826 Genomic DNA. Translation: CAI12827.1. CH471090 Genomic DNA. Translation: EAW88056.1. CH471090 Genomic DNA. Translation: EAW88060.1. BC024225 mRNA. Translation: AAH24225.1. BC040111 mRNA. Translation: AAH40111.1. |
CCDSi | CCDS6963.1. [Q15528-1] CCDS6964.1. [Q15528-2] |
RefSeqi | NP_598395.1. NM_133640.4. [Q15528-1] NP_852468.1. NM_181491.2. [Q15528-2] |
UniGenei | Hs.78354. |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | MED22_HUMAN | |
Accessioni | Q15528Primary (citable) accession number: Q15528 Secondary accession number(s): B3KW83 Q5T8U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | July 15, 1999 | |
Last modified: | March 28, 2018 | |
This is version 148 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |