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Protein

Regucalcin

Gene

RGN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca2+ signaling, and Ca2+-dependent cellular processes and enzyme activities (By similarity).By similarity

Catalytic activityi

D-glucono-1,5-lactone + H2O = D-gluconate.2 Publications

Cofactori

Zn2+2 Publications, Mn2+2 Publications, Ca2+2 Publications, Mg2+2 PublicationsNote: Binds 1 divalent metal cation per subunit. Most active with Zn2+ and Mn2+ ions. The physiological cofactor is most likely Ca2+ or Mg2+.2 Publications

Kineticsi

  1. KM=2.7 mM for gluconolactone (with Zn2+ as cofactor)1 Publication
  2. KM=0.6 mM for gluconolactone (with Mn2+ as cofactor)1 Publication
  3. KM=1.3 mM for gluconolactone (with Mg2+ as cofactor)1 Publication
  4. KM=3.7 mM for gluconolactone (with Ca2+ as cofactor)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi18Divalent metal cation1 Publication1
    Binding sitei101Substrate1
    Binding sitei103Substrate1
    Binding sitei121SubstrateBy similarity1
    Metal bindingi154Divalent metal cation1 Publication1
    Active sitei204Proton donor/acceptor1 Publication1
    Metal bindingi204Divalent metal cation1 Publication1

    GO - Molecular functioni

    • calcium ion binding Source: UniProtKB
    • enzyme regulator activity Source: InterPro
    • gluconolactonase activity Source: UniProtKB
    • zinc ion binding Source: UniProtKB

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000130988-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regucalcin
    Short name:
    RC
    Alternative name(s):
    Gluconolactonase (EC:3.1.1.17)
    Short name:
    GNL
    Senescence marker protein 30
    Short name:
    SMP-30
    Gene namesi
    Name:RGN
    Synonyms:SMP30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome X

    Organism-specific databases

    HGNCiHGNC:9989. RGN.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi18E → A: Reduces enzyme activity by about 90%. 1 Publication1
    Mutagenesisi103N → A: Reduces enzyme activity by about 95%. 1 Publication1
    Mutagenesisi154N → A: Reduces enzyme activity by about 95%. 1 Publication1
    Mutagenesisi204D → A: Reduces enzyme activity by over 98%. 1 Publication1

    Organism-specific databases

    DisGeNETi9104.
    OpenTargetsiENSG00000130988.
    PharmGKBiPA34359.

    Polymorphism and mutation databases

    BioMutaiRGN.
    DMDMi3334328.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001730461 – 299RegucalcinAdd BLAST299

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei144N6-succinyllysineBy similarity1
    Modified residuei244N6-succinyllysineBy similarity1
    Modified residuei253N6-succinyllysineBy similarity1

    Proteomic databases

    PaxDbiQ15493.
    PeptideAtlasiQ15493.
    PRIDEiQ15493.

    PTM databases

    iPTMnetiQ15493.
    PhosphoSitePlusiQ15493.

    Expressioni

    Gene expression databases

    BgeeiENSG00000130988.
    CleanExiHS_RGN.
    ExpressionAtlasiQ15493. baseline and differential.
    GenevisibleiQ15493. HS.

    Organism-specific databases

    HPAiHPA029102.
    HPA029103.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi114557. 7 interactors.
    MINTiMINT-3031653.
    STRINGi9606.ENSP00000253303.

    Structurei

    Secondary structure

    1299
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi5 – 10Combined sources6
    Beta strandi15 – 23Combined sources9
    Turni24 – 27Combined sources4
    Beta strandi28 – 33Combined sources6
    Turni34 – 37Combined sources4
    Beta strandi38 – 43Combined sources6
    Turni44 – 46Combined sources3
    Beta strandi49 – 53Combined sources5
    Beta strandi58 – 64Combined sources7
    Beta strandi67 – 73Combined sources7
    Beta strandi76 – 81Combined sources6
    Turni82 – 85Combined sources4
    Beta strandi86 – 92Combined sources7
    Beta strandi98 – 107Combined sources10
    Beta strandi113 – 119Combined sources7
    Beta strandi132 – 137Combined sources6
    Beta strandi143 – 158Combined sources16
    Beta strandi164 – 169Combined sources6
    Helixi170 – 172Combined sources3
    Beta strandi174 – 180Combined sources7
    Turni182 – 184Combined sources3
    Beta strandi187 – 195Combined sources9
    Helixi198 – 200Combined sources3
    Beta strandi202 – 209Combined sources8
    Beta strandi214 – 219Combined sources6
    Turni220 – 222Combined sources3
    Beta strandi223 – 227Combined sources5
    Turni229 – 231Combined sources3
    Beta strandi234 – 239Combined sources6
    Beta strandi241 – 243Combined sources3
    Beta strandi245 – 252Combined sources8
    Helixi253 – 255Combined sources3
    Beta strandi257 – 263Combined sources7
    Helixi269 – 274Combined sources6
    Turni276 – 279Combined sources4
    Beta strandi281 – 285Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3G4EX-ray1.42A/B3-299[»]
    3G4HX-ray1.92A/B3-299[»]
    4GNBX-ray1.50A/B1-299[»]
    4GNCX-ray1.75A/B1-299[»]
    ProteinModelPortaliQ15493.
    SMRiQ15493.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ15493.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SMP-30/CGR1 family.Curated

    Phylogenomic databases

    eggNOGiKOG4499. Eukaryota.
    COG3386. LUCA.
    GeneTreeiENSGT00390000014995.
    HOGENOMiHOG000220627.
    HOVERGENiHBG004347.
    InParanoidiQ15493.
    KOiK01053.
    OMAiCARDGMD.
    OrthoDBiEOG091G131G.
    PhylomeDBiQ15493.
    TreeFamiTF323663.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR008367. Regucalcin.
    IPR013658. SGL.
    IPR005511. SMP-30.
    [Graphical view]
    PfamiPF08450. SGL. 1 hit.
    [Graphical view]
    PRINTSiPR01791. REGUCALCIN.
    PR01790. SMP30FAMILY.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q15493-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ
    60 70 80 90 100
    RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN
    110 120 130 140 150
    RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV
    160 170 180 190 200
    DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE
    210 220 230 240 250
    QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF
    260 270 280 290
    GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG
    Length:299
    Mass (Da):33,253
    Last modified:November 1, 1996 - v1
    Checksum:i95BA1C73B7B77635
    GO
    Isoform 2 (identifier: Q15493-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         116-187: Missing.
         188-188: S → A

    Note: No experimental confirmation available.
    Show »
    Length:227
    Mass (Da):24,968
    Checksum:i19BC938A52DA72D4
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti292 – 293IA → TS in BAD97080 (Ref. 4) Curated2

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_025456116 – 187Missing in isoform 2. 1 PublicationAdd BLAST72
    Alternative sequenceiVSP_025457188S → A in isoform 2. 1 Publication1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D31815 mRNA. Translation: BAA06602.1.
    AB028125 mRNA. Translation: BAA78693.1.
    AB032064 mRNA. Translation: BAA84082.1.
    AK290136 mRNA. Translation: BAF82825.1.
    AK223360 mRNA. Translation: BAD97080.1.
    AL513366 Genomic DNA. Translation: CAI41697.1.
    BC050371 mRNA. Translation: AAH50371.1.
    BC073173 mRNA. Translation: AAH73173.1.
    CCDSiCCDS14272.1. [Q15493-1]
    CCDS75968.1. [Q15493-2]
    PIRiS60035.
    RefSeqiNP_001269777.1. NM_001282848.1.
    NP_001269778.1. NM_001282849.1. [Q15493-2]
    NP_004674.1. NM_004683.5. [Q15493-1]
    NP_690608.1. NM_152869.3. [Q15493-1]
    XP_006724630.1. XM_006724567.2. [Q15493-2]
    UniGeneiHs.77854.

    Genome annotation databases

    EnsembliENST00000336169; ENSP00000338400; ENSG00000130988. [Q15493-1]
    ENST00000352078; ENSP00000253303; ENSG00000130988. [Q15493-1]
    ENST00000397180; ENSP00000380365; ENSG00000130988. [Q15493-1]
    ENST00000457380; ENSP00000406568; ENSG00000130988. [Q15493-2]
    GeneIDi9104.
    KEGGihsa:9104.
    UCSCiuc010nhp.2. human. [Q15493-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D31815 mRNA. Translation: BAA06602.1.
    AB028125 mRNA. Translation: BAA78693.1.
    AB032064 mRNA. Translation: BAA84082.1.
    AK290136 mRNA. Translation: BAF82825.1.
    AK223360 mRNA. Translation: BAD97080.1.
    AL513366 Genomic DNA. Translation: CAI41697.1.
    BC050371 mRNA. Translation: AAH50371.1.
    BC073173 mRNA. Translation: AAH73173.1.
    CCDSiCCDS14272.1. [Q15493-1]
    CCDS75968.1. [Q15493-2]
    PIRiS60035.
    RefSeqiNP_001269777.1. NM_001282848.1.
    NP_001269778.1. NM_001282849.1. [Q15493-2]
    NP_004674.1. NM_004683.5. [Q15493-1]
    NP_690608.1. NM_152869.3. [Q15493-1]
    XP_006724630.1. XM_006724567.2. [Q15493-2]
    UniGeneiHs.77854.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3G4EX-ray1.42A/B3-299[»]
    3G4HX-ray1.92A/B3-299[»]
    4GNBX-ray1.50A/B1-299[»]
    4GNCX-ray1.75A/B1-299[»]
    ProteinModelPortaliQ15493.
    SMRiQ15493.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114557. 7 interactors.
    MINTiMINT-3031653.
    STRINGi9606.ENSP00000253303.

    PTM databases

    iPTMnetiQ15493.
    PhosphoSitePlusiQ15493.

    Polymorphism and mutation databases

    BioMutaiRGN.
    DMDMi3334328.

    Proteomic databases

    PaxDbiQ15493.
    PeptideAtlasiQ15493.
    PRIDEiQ15493.

    Protocols and materials databases

    DNASUi9104.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000336169; ENSP00000338400; ENSG00000130988. [Q15493-1]
    ENST00000352078; ENSP00000253303; ENSG00000130988. [Q15493-1]
    ENST00000397180; ENSP00000380365; ENSG00000130988. [Q15493-1]
    ENST00000457380; ENSP00000406568; ENSG00000130988. [Q15493-2]
    GeneIDi9104.
    KEGGihsa:9104.
    UCSCiuc010nhp.2. human. [Q15493-1]

    Organism-specific databases

    CTDi9104.
    DisGeNETi9104.
    GeneCardsiRGN.
    HGNCiHGNC:9989. RGN.
    HPAiHPA029102.
    HPA029103.
    MIMi300212. gene.
    neXtProtiNX_Q15493.
    OpenTargetsiENSG00000130988.
    PharmGKBiPA34359.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG4499. Eukaryota.
    COG3386. LUCA.
    GeneTreeiENSGT00390000014995.
    HOGENOMiHOG000220627.
    HOVERGENiHBG004347.
    InParanoidiQ15493.
    KOiK01053.
    OMAiCARDGMD.
    OrthoDBiEOG091G131G.
    PhylomeDBiQ15493.
    TreeFamiTF323663.

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000130988-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ15493.
    GeneWikiiRGN_(gene).
    GenomeRNAii9104.
    PROiQ15493.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000130988.
    CleanExiHS_RGN.
    ExpressionAtlasiQ15493. baseline and differential.
    GenevisibleiQ15493. HS.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR008367. Regucalcin.
    IPR013658. SGL.
    IPR005511. SMP-30.
    [Graphical view]
    PfamiPF08450. SGL. 1 hit.
    [Graphical view]
    PRINTSiPR01791. REGUCALCIN.
    PR01790. SMP30FAMILY.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRGN_HUMAN
    AccessioniPrimary (citable) accession number: Q15493
    Secondary accession number(s): A4FTW1
    , A8K271, Q53FC9, Q5JRR5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Gluconolactonase catalyzes a key step in ascorbic acid (vitamin C) biosynthesis, but primates lack the last enzyme in the pathway and are unable to synthesize vitamin C.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.