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Protein

Ficolin-2

Gene

FCN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi249CalciumCombined sources1 Publication1
Metal bindingi251CalciumCombined sources1 Publication1
Metal bindingi253Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi255Calcium; via carbonyl oxygenCombined sources1 Publication1

GO - Molecular functioni

  • antigen binding Source: UniProtKB
  • calcium-dependent protein binding Source: UniProtKB
  • mannan binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • proteoglycan binding Source: UniProtKB
  • serine-type endopeptidase activity Source: Reactome

GO - Biological processi

  • complement activation Source: Reactome
  • complement activation, lectin pathway Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • opsonization Source: UniProtKB
  • recognition of apoptotic cell Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Complement activation lectin pathway, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Lectin, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160339-MONOMER.
ReactomeiR-HSA-166662. Lectin pathway of complement activation.
R-HSA-166663. Initial triggering of complement.
R-HSA-2855086. Ficolins bind to repetitive carbohydrate structures on the target cell surface.

Names & Taxonomyi

Protein namesi
Recommended name:
Ficolin-2
Alternative name(s):
37 kDa elastin-binding protein
Collagen/fibrinogen domain-containing protein 2
EBP-37
Ficolin-B
Ficolin-beta
Hucolin
L-ficolin
Serum lectin p35
Gene namesi
Name:FCN2
Synonyms:FCNL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:3624. FCN2.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: UniProtKB
  • collagen trimer Source: UniProtKB-KW
  • extracellular exosome Source: UniProtKB
  • extracellular region Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi2220.
OpenTargetsiENSG00000160339.
PharmGKBiPA28070.

Polymorphism and mutation databases

BioMutaiFCN2.
DMDMi166214934.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000913926 – 313Ficolin-2Add BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei77Hydroxyproline1 Publication1
Modified residuei80Hydroxyproline1 Publication1
Modified residuei86Hydroxyproline1 Publication1
Disulfide bondi98 ↔ 126By similarity
Disulfide bondi105 ↔ 133Combined sources1 Publication
Glycosylationi240N-linked (GlcNAc...)Sequence analysis1 Publication1
Disulfide bondi257 ↔ 270Combined sources1 Publication
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

MaxQBiQ15485.
PaxDbiQ15485.
PeptideAtlasiQ15485.
PRIDEiQ15485.

Expressioni

Tissue specificityi

Expressed by the liver and secreted in plasma.

Gene expression databases

BgeeiENSG00000160339.
CleanExiHS_FCN2.
GenevisibleiQ15485. HS.

Interactioni

Subunit structurei

Homotrimer (PubMed:17215869). Interacts with elastin. Interacts with MASP1 and MASP2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LRP1Q079542EBI-7468784,EBI-1046087
PTX3P260227EBI-7468784,EBI-11574553

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108514. 3 interactors.
IntActiQ15485. 5 interactors.
MINTiMINT-5223224.
STRINGi9606.ENSP00000291744.

Structurei

Secondary structure

1313
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni98 – 100Combined sources3
Helixi105 – 110Combined sources6
Beta strandi115 – 122Combined sources8
Beta strandi124 – 126Combined sources3
Beta strandi128 – 134Combined sources7
Helixi137 – 139Combined sources3
Beta strandi142 – 151Combined sources10
Helixi159 – 164Combined sources6
Beta strandi166 – 168Combined sources3
Helixi177 – 182Combined sources6
Beta strandi185 – 187Combined sources3
Beta strandi191 – 196Combined sources6
Beta strandi198 – 200Combined sources3
Beta strandi202 – 208Combined sources7
Beta strandi210 – 212Combined sources3
Turni215 – 219Combined sources5
Beta strandi221 – 223Combined sources3
Beta strandi226 – 228Combined sources3
Helixi236 – 238Combined sources3
Beta strandi246 – 248Combined sources3
Beta strandi251 – 255Combined sources5
Helixi258 – 261Combined sources4
Beta strandi263 – 265Combined sources3
Beta strandi268 – 270Combined sources3
Beta strandi272 – 274Combined sources3
Beta strandi285 – 288Combined sources4
Beta strandi290 – 293Combined sources4
Turni294 – 297Combined sources4
Beta strandi299 – 302Combined sources4
Beta strandi304 – 310Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J0GX-ray2.85A/B/C/D/E/F97-313[»]
2J0HX-ray2.85A/B/C/D/E/F97-313[»]
2J0YX-ray2.35A/B/C/D/E/F97-313[»]
2J1GX-ray1.95A/B/C/D/E/F97-313[»]
2J2PX-ray2.80A/B/C/D/E/F97-313[»]
2J3FX-ray2.80A/B/C/D/E/F95-313[»]
2J3GX-ray2.50A/B/C/D/E/F97-313[»]
2J3OX-ray2.65A/B/C/D/E/F95-313[»]
2J3UX-ray2.15A/B/C/D/E/F97-313[»]
2J61X-ray2.70A/B97-313[»]
4NYTX-ray2.25A/B/C97-313[»]
4R9JX-ray2.10A/B/G97-313[»]
4R9TX-ray2.25A/B/C97-313[»]
ProteinModelPortaliQ15485.
SMRiQ15485.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15485.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 92Collagen-likeAdd BLAST42
Domaini96 – 313Fibrinogen C-terminalPROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni241 – 242Carbohydrate binding1 Publication2

Domaini

The fibrinogen-like domain (FBG) contains calcium-binding sites that may be involved in carbohydrate binding.1 Publication

Sequence similaritiesi

Belongs to the ficolin lectin family.Curated
Contains 1 collagen-like domain.Curated
Contains 1 fibrinogen C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000037127.
HOVERGENiHBG001644.
InParanoidiQ15485.
KOiK10104.
OMAiNTGNCAV.
OrthoDBiEOG091G03M1.
PhylomeDBiQ15485.
TreeFamiTF329953.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR008160. Collagen.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15485-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELDRAVGVL GAATLLLSFL GMAWALQAAD TCPEVKMVGL EGSDKLTILR
60 70 80 90 100
GCPGLPGAPG PKGEAGTNGK RGERGPPGPP GKAGPPGPNG APGEPQPCLT
110 120 130 140 150
GPRTCKDLLD RGHFLSGWHT IYLPDCRPLT VLCDMDTDGG GWTVFQRRVD
160 170 180 190 200
GSVDFYRDWA TYKQGFGSRL GEFWLGNDNI HALTAQGTSE LRVDLVDFED
210 220 230 240 250
NYQFAKYRSF KVADEAEKYN LVLGAFVEGS AGDSLTFHNN QSFSTKDQDN
260 270 280 290 300
DLNTGNCAVM FQGAWWYKNC HVSNLNGRYL RGTHGSFANG INWKSGKGYN
310
YSYKVSEMKV RPA
Length:313
Mass (Da):34,001
Last modified:January 15, 2008 - v2
Checksum:iD363029846CCB3C9
GO
Isoform 2 (identifier: Q15485-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     34-71: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):30,228
Checksum:i033A008F64CFA4C0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61P → D in BAA08352 (PubMed:8884275).Curated1
Sequence conflicti61P → D in ABB01005 (PubMed:8576206).Curated1
Sequence conflicti98C → A AA sequence (PubMed:8586615).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03634280P → L in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_049072113H → Y.1 PublicationCorresponds to variant rs17549179dbSNPEnsembl.1
Natural variantiVAR_049073117G → S.Corresponds to variant rs12684476dbSNPEnsembl.1
Natural variantiVAR_049074236T → M.Corresponds to variant rs17549193dbSNPEnsembl.1
Natural variantiVAR_049075258A → S.Corresponds to variant rs7851696dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03002734 – 71Missing in isoform 2. CuratedAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63160 Genomic DNA. Translation: BAA09636.1.
D49353 mRNA. Translation: BAA08352.1.
DQ217935 Genomic DNA. Translation: ABB01005.1.
AK290843 mRNA. Translation: BAF83532.1.
AL603650 Genomic DNA. Translation: CAI39861.1.
CH471090 Genomic DNA. Translation: EAW88133.1.
CH471090 Genomic DNA. Translation: EAW88135.1.
BC069572 mRNA. Translation: AAH69572.1.
BC069825 mRNA. Translation: AAH69825.1.
CCDSiCCDS6983.1. [Q15485-1]
PIRiPH0263.
S68005.
RefSeqiNP_004099.2. NM_004108.2. [Q15485-1]
NP_056652.1. NM_015837.2. [Q15485-2]
UniGeneiHs.54517.

Genome annotation databases

EnsembliENST00000291744; ENSP00000291744; ENSG00000160339. [Q15485-1]
ENST00000350339; ENSP00000291741; ENSG00000160339. [Q15485-2]
GeneIDi2220.
KEGGihsa:2220.
UCSCiuc004cfg.1. human. [Q15485-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63160 Genomic DNA. Translation: BAA09636.1.
D49353 mRNA. Translation: BAA08352.1.
DQ217935 Genomic DNA. Translation: ABB01005.1.
AK290843 mRNA. Translation: BAF83532.1.
AL603650 Genomic DNA. Translation: CAI39861.1.
CH471090 Genomic DNA. Translation: EAW88133.1.
CH471090 Genomic DNA. Translation: EAW88135.1.
BC069572 mRNA. Translation: AAH69572.1.
BC069825 mRNA. Translation: AAH69825.1.
CCDSiCCDS6983.1. [Q15485-1]
PIRiPH0263.
S68005.
RefSeqiNP_004099.2. NM_004108.2. [Q15485-1]
NP_056652.1. NM_015837.2. [Q15485-2]
UniGeneiHs.54517.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J0GX-ray2.85A/B/C/D/E/F97-313[»]
2J0HX-ray2.85A/B/C/D/E/F97-313[»]
2J0YX-ray2.35A/B/C/D/E/F97-313[»]
2J1GX-ray1.95A/B/C/D/E/F97-313[»]
2J2PX-ray2.80A/B/C/D/E/F97-313[»]
2J3FX-ray2.80A/B/C/D/E/F95-313[»]
2J3GX-ray2.50A/B/C/D/E/F97-313[»]
2J3OX-ray2.65A/B/C/D/E/F95-313[»]
2J3UX-ray2.15A/B/C/D/E/F97-313[»]
2J61X-ray2.70A/B97-313[»]
4NYTX-ray2.25A/B/C97-313[»]
4R9JX-ray2.10A/B/G97-313[»]
4R9TX-ray2.25A/B/C97-313[»]
ProteinModelPortaliQ15485.
SMRiQ15485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108514. 3 interactors.
IntActiQ15485. 5 interactors.
MINTiMINT-5223224.
STRINGi9606.ENSP00000291744.

Polymorphism and mutation databases

BioMutaiFCN2.
DMDMi166214934.

Proteomic databases

MaxQBiQ15485.
PaxDbiQ15485.
PeptideAtlasiQ15485.
PRIDEiQ15485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291744; ENSP00000291744; ENSG00000160339. [Q15485-1]
ENST00000350339; ENSP00000291741; ENSG00000160339. [Q15485-2]
GeneIDi2220.
KEGGihsa:2220.
UCSCiuc004cfg.1. human. [Q15485-1]

Organism-specific databases

CTDi2220.
DisGeNETi2220.
GeneCardsiFCN2.
HGNCiHGNC:3624. FCN2.
MIMi601624. gene.
neXtProtiNX_Q15485.
OpenTargetsiENSG00000160339.
PharmGKBiPA28070.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2579. Eukaryota.
ENOG410ZYS4. LUCA.
GeneTreeiENSGT00830000128240.
HOGENOMiHOG000037127.
HOVERGENiHBG001644.
InParanoidiQ15485.
KOiK10104.
OMAiNTGNCAV.
OrthoDBiEOG091G03M1.
PhylomeDBiQ15485.
TreeFamiTF329953.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000160339-MONOMER.
ReactomeiR-HSA-166662. Lectin pathway of complement activation.
R-HSA-166663. Initial triggering of complement.
R-HSA-2855086. Ficolins bind to repetitive carbohydrate structures on the target cell surface.

Miscellaneous databases

EvolutionaryTraceiQ15485.
GeneWikiiFCN2.
GenomeRNAii2220.
PROiQ15485.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160339.
CleanExiHS_FCN2.
GenevisibleiQ15485. HS.

Family and domain databases

CDDicd00087. FReD. 1 hit.
Gene3Di3.90.215.10. 1 hit.
4.10.530.10. 1 hit.
InterProiIPR008160. Collagen.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR002181. Fibrinogen_a/b/g_C_dom.
IPR020837. Fibrinogen_CS.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTiSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMiSSF56496. SSF56496. 1 hit.
PROSITEiPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFCN2_HUMAN
AccessioniPrimary (citable) accession number: Q15485
Secondary accession number(s): A6NFG7
, A8K478, Q6IS69, Q7M4P4, Q9UC57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 15, 2008
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.