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Protein

Cytohesin-1

Gene

CYTH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei292Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei302Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei351Phosphatidylinositol 3,4,5-trisphosphateBy similarity1

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • regulation of ARF protein signal transduction Source: InterPro
  • regulation of cell adhesion Source: MGI
  • vesicle-mediated transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108669-MONOMER.
ReactomeiR-HSA-6811438. Intra-Golgi traffic.
SIGNORiQ15438.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-1
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
SEC7 homolog B2-1
Gene namesi
Name:CYTH1
Synonyms:D17S811E, PSCD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:9501. CYTH1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
  • Golgi membrane Source: Reactome
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157E → A or K: Reduces guanine exchange factor activity by over 90%. 1 Publication1
Mutagenesisi187Y → A: Reduces guanine exchange factor activity by over 90%. 1 Publication1
Mutagenesisi195M → A: Reduces guanine exchange factor activity by over 90%. 1 Publication1

Organism-specific databases

DisGeNETi9267.
OpenTargetsiENSG00000108669.
PharmGKBiPA164718528.

Polymorphism and mutation databases

BioMutaiCYTH1.
DMDMi2498175.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201941 – 398Cytohesin-1Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ15438.
MaxQBiQ15438.
PaxDbiQ15438.
PeptideAtlasiQ15438.
PRIDEiQ15438.

PTM databases

iPTMnetiQ15438.
PhosphoSitePlusiQ15438.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000108669.
CleanExiHS_CYTH1.
ExpressionAtlasiQ15438. baseline and differential.
GenevisibleiQ15438. HS.

Organism-specific databases

HPAiHPA047498.

Interactioni

Subunit structurei

Interacts with TRIM23 and CYTIP.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCDC120Q96HB54EBI-997830,EBI-744556
CYTIPO607592EBI-997830,EBI-997814

Protein-protein interaction databases

BioGridi114688. 19 interactors.
IntActiQ15438. 11 interactors.
MINTiMINT-3031502.
STRINGi9606.ENSP00000354398.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 75Combined sources12
Helixi77 – 86Combined sources10
Helixi94 – 103Combined sources10
Helixi109 – 116Combined sources8
Helixi121 – 133Combined sources13
Turni137 – 139Combined sources3
Helixi141 – 150Combined sources10
Helixi158 – 175Combined sources18
Helixi183 – 201Combined sources19
Beta strandi203 – 205Combined sources3
Helixi211 – 217Combined sources7
Turni218 – 221Combined sources4
Helixi229 – 241Combined sources13
Beta strandi246 – 248Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BC9NMR-A58-256[»]
4A4PX-ray2.00A/B63-248[»]
ProteinModelPortaliQ15438.
SMRiQ15438.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15438.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 202SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini260 – 377PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni269 – 277Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity9
Regioni388 – 396C-terminal autoinhibitory regionBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 67Sequence analysisAdd BLAST58

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.By similarity

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SEC7 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiQ15438.
KOiK18441.
OMAiMVLKTEE.
OrthoDBiEOG091G0RZS.
PhylomeDBiQ15438.
TreeFamiTF352091.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15438-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKDE IAEVANEIEN
60 70 80 90 100
LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENDLL KNTCEDIAQF
110 120 130 140 150
LYKGEGLNKT AIGDYLGERD EFNIQVLHAF VELHEFTDLN LVQALRQFLW
160 170 180 190 200
SFRLPGEAQK IDRMMEAFAQ RYCQCNNGVF QSTDTCYVLS FAIIMLNTSL
210 220 230 240 250
HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD
260 270 280 290 300
GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE
310 320 330 340 350
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG
360 370 380 390
NHTVYRISAP TPEEKEEWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
Length:398
Mass (Da):46,413
Last modified:November 1, 1996 - v1
Checksum:i067FEE0FEA7A4C86
GO
Isoform 2 (identifier: Q15438-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Show »
Length:397
Mass (Da):46,356
Checksum:iCBD984BC348E6B15
GO
Isoform 3 (identifier: Q15438-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-59: Missing.

Note: No experimental confirmation available.
Show »
Length:339
Mass (Da):39,470
Checksum:iC08318AD7469BD02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353 – 361TVYRISAPT → MFTGSQLRR in AAF37737 (PubMed:15489334).Curated9
Sequence conflicti353 – 361TVYRISAPT → MFTGSQLRR in AAF37738 (PubMed:15489334).Curated9

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0558841 – 59Missing in isoform 3. 1 PublicationAdd BLAST59
Alternative sequenceiVSP_006034273Missing in isoform 2. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85169 mRNA. Translation: AAA36602.1.
AK293894 mRNA. Translation: BAH11620.1.
AK316277 mRNA. Translation: BAH14648.1.
AC022966 Genomic DNA. No translation available.
AC099804 Genomic DNA. No translation available.
BC038385 mRNA. Translation: AAH38385.1.
BC050452 mRNA. Translation: AAH50452.1.
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37737.1.
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125358, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37738.1.
CCDSiCCDS32754.1. [Q15438-2]
CCDS42392.2. [Q15438-1]
PIRiS24168.
RefSeqiNP_001278947.1. NM_001292018.1. [Q15438-3]
NP_001278948.1. NM_001292019.1. [Q15438-3]
NP_004753.1. NM_004762.3. [Q15438-1]
NP_059430.2. NM_017456.3. [Q15438-2]
XP_011523779.1. XM_011525477.1. [Q15438-3]
UniGeneiHs.191215.

Genome annotation databases

EnsembliENST00000361101; ENSP00000354398; ENSG00000108669. [Q15438-1]
ENST00000446868; ENSP00000389095; ENSG00000108669. [Q15438-1]
ENST00000585509; ENSP00000465940; ENSG00000108669. [Q15438-3]
ENST00000589297; ENSP00000466512; ENSG00000108669. [Q15438-3]
ENST00000591455; ENSP00000465665; ENSG00000108669. [Q15438-2]
GeneIDi9267.
KEGGihsa:9267.
UCSCiuc002jvw.4. human. [Q15438-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85169 mRNA. Translation: AAA36602.1.
AK293894 mRNA. Translation: BAH11620.1.
AK316277 mRNA. Translation: BAH14648.1.
AC022966 Genomic DNA. No translation available.
AC099804 Genomic DNA. No translation available.
BC038385 mRNA. Translation: AAH38385.1.
BC050452 mRNA. Translation: AAH50452.1.
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37737.1.
AF125362
, AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125358, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37738.1.
CCDSiCCDS32754.1. [Q15438-2]
CCDS42392.2. [Q15438-1]
PIRiS24168.
RefSeqiNP_001278947.1. NM_001292018.1. [Q15438-3]
NP_001278948.1. NM_001292019.1. [Q15438-3]
NP_004753.1. NM_004762.3. [Q15438-1]
NP_059430.2. NM_017456.3. [Q15438-2]
XP_011523779.1. XM_011525477.1. [Q15438-3]
UniGeneiHs.191215.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BC9NMR-A58-256[»]
4A4PX-ray2.00A/B63-248[»]
ProteinModelPortaliQ15438.
SMRiQ15438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114688. 19 interactors.
IntActiQ15438. 11 interactors.
MINTiMINT-3031502.
STRINGi9606.ENSP00000354398.

PTM databases

iPTMnetiQ15438.
PhosphoSitePlusiQ15438.

Polymorphism and mutation databases

BioMutaiCYTH1.
DMDMi2498175.

Proteomic databases

EPDiQ15438.
MaxQBiQ15438.
PaxDbiQ15438.
PeptideAtlasiQ15438.
PRIDEiQ15438.

Protocols and materials databases

DNASUi9267.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361101; ENSP00000354398; ENSG00000108669. [Q15438-1]
ENST00000446868; ENSP00000389095; ENSG00000108669. [Q15438-1]
ENST00000585509; ENSP00000465940; ENSG00000108669. [Q15438-3]
ENST00000589297; ENSP00000466512; ENSG00000108669. [Q15438-3]
ENST00000591455; ENSP00000465665; ENSG00000108669. [Q15438-2]
GeneIDi9267.
KEGGihsa:9267.
UCSCiuc002jvw.4. human. [Q15438-1]

Organism-specific databases

CTDi9267.
DisGeNETi9267.
GeneCardsiCYTH1.
HGNCiHGNC:9501. CYTH1.
HPAiHPA047498.
MIMi182115. gene.
neXtProtiNX_Q15438.
OpenTargetsiENSG00000108669.
PharmGKBiPA164718528.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOGENOMiHOG000253023.
HOVERGENiHBG002647.
InParanoidiQ15438.
KOiK18441.
OMAiMVLKTEE.
OrthoDBiEOG091G0RZS.
PhylomeDBiQ15438.
TreeFamiTF352091.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000108669-MONOMER.
ReactomeiR-HSA-6811438. Intra-Golgi traffic.
SIGNORiQ15438.

Miscellaneous databases

ChiTaRSiCYTH1. human.
EvolutionaryTraceiQ15438.
GeneWikiiCYTH1.
GenomeRNAii9267.
PROiQ15438.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108669.
CleanExiHS_CYTH1.
ExpressionAtlasiQ15438. baseline and differential.
GenevisibleiQ15438. HS.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYH1_HUMAN
AccessioniPrimary (citable) accession number: Q15438
Secondary accession number(s): A6NFW7
, B7Z1T4, Q9P123, Q9P124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.