Q15438 (CYH1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytohesin-1

Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
SEC7 homolog B2-1

Gene names

Name:	CYTH1
Synonyms:	D17S811E, PSCD1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
Subunit structure	Interacts with TRIM23 and CYTIP. Ref.5 Ref.6
Subcellular location	Cell membrane Ref.7.
Tissue specificity	Ubiquitous.
Sequence similarities	Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of epithelial cell polarity Inferred from electronic annotation. Source: Compara regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell adhesion Inferred from direct assay PubMed 12606567. Source: MGI vesicle-mediated transport Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay PubMed 12606567. Source: MGI plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB tight junction Inferred from electronic annotation. Source: Compara
Molecular_function	ARF guanyl-nucleotide exchange factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CYTIP	O60759	2	EBI-997830,EBI-997814

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15438-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15438-2)

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 398

398

Cytohesin-1

PRO_0000120194

Regions

Domain

73 – 202

130

SEC7

Domain

260 – 377

118

PH

Coiled coil

10 – 67

58

Potential

Natural variations

Alternative sequence

273

1

Missing in isoform 2.

VSP_006034

Experimental info

Sequence conflict

353 – 361

9

TVYRISAPT → MFTGSQLRR in AAF37737. Ref.4

Sequence conflict

353 – 361

9

TVYRISAPT → MFTGSQLRR in AAF37738. Ref.4

Secondary structure

1

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398

Helix Strand Turn

Details...

Helix

64 – 75

12

Helix

77 – 86

10

Helix

94 – 103

10

Helix

109 – 116

8

Helix

121 – 133

13

Turn

137 – 139

3

Helix

141 – 150

10

Helix

158 – 175

18

Helix

183 – 201

19

Beta strand

203 – 205

3

Helix

211 – 217

7

Turn

218 – 221

4

Helix

229 – 241

13

Beta strand

246 – 248

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 067FEE0FEA7A4C86	FASTA	398	46,413
	10 20 30 40 50 60 MEEDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKDE IAEVANEIEN LGSTEERKNM 70 80 90 100 110 120 QRNKQVAMGR KKFNMDPKKG IQFLIENDLL KNTCEDIAQF LYKGEGLNKT AIGDYLGERD 130 140 150 160 170 180 EFNIQVLHAF VELHEFTDLN LVQALRQFLW SFRLPGEAQK IDRMMEAFAQ RYCQCNNGVF 190 200 210 220 230 240 QSTDTCYVLS FAIIMLNTSL HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK 250 260 270 280 290 300 NEPFKIPEDD GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE 310 320 330 340 350 360 PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG NHTVYRISAP 370 380 390 TPEEKEEWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
	Isoform 2 [UniParc]. Checksum: CBD984BC348E6B15	FASTA	397	46,356
	10 20 30 40 50 60 MEEDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKDE IAEVANEIEN LGSTEERKNM 70 80 90 100 110 120 QRNKQVAMGR KKFNMDPKKG IQFLIENDLL KNTCEDIAQF LYKGEGLNKT AIGDYLGERD 130 140 150 160 170 180 EFNIQVLHAF VELHEFTDLN LVQALRQFLW SFRLPGEAQK IDRMMEAFAQ RYCQCNNGVF 190 200 210 220 230 240 QSTDTCYVLS FAIIMLNTSL HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK 250 260 270 280 290 300 NEPFKIPEDD GNDLTHTFFN PDREGWLLKL GGRVKTWKRR WFILTDNCLY YFEYTTDKEP 310 320 330 340 350 360 RGIIPLENLS IREVEDSKKP NCFELYIPDN KDQVIKACKT EADGRVVEGN HTVYRISAPT 370 380 390 PEEKEEWIKC IKAAISRDPF YEMLAARKKK VSSTKRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a human cDNA from cytolytic NK/T cells with homology to yeast SEC7." Liu L., Pohajdak B. Biochim. Biophys. Acta 1132:75-78(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin and Testis.
[4]	"Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors." Ogasawara M., Kim S.C., Adamik R., Togawa A., Ferrans V.J., Takeda K., Kirby M., Moss J., Vaughan M. J. Biol. Chem. 275:3221-3230(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-398, ALTERNATIVE SPLICING.
[5]	"Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)." Vitale N., Pacheco-Rodriguez G., Ferrans V.J., Riemenschneider W., Moss J., Vaughan M. J. Biol. Chem. 275:21331-21339(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH TRIM23.
[6]	"Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity." Tang P., Cheng T.P., Agnello D., Wu C.-Y., Hissong B.D., Watford W.T., Ahn H.-J., Galon J., Moss J., Vaughan M., O'Shea J.J., Gadina M. Proc. Natl. Acad. Sci. U.S.A. 99:2625-2629(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CYTIP.
[7]	"The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane." Hofmann I., Thompson A., Sanderson C.M., Munro S. Curr. Biol. 17:711-716(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Solution structure of the cytohesin-1 (B2-1) Sec7 domain and its interaction with the GTPase ADP ribosylation factor 1." Betz S.F., Schnuchel A., Wang H., Olejniczak E.T., Meadows R.P., Lipsky B.P., Harris E.A., Staunton D.E., Fesik S.W. Proc. Natl. Acad. Sci. U.S.A. 95:7909-7914(1998) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 58-256.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M85169 mRNA. Translation: AAA36602.1.
AC022966 Genomic DNA. No translation available.
BC038385 mRNA. Translation: AAH38385.1.
BC050452 mRNA. Translation: AAH50452.1.
AF125362 , AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37737.1.
AF125362 , AF125350, AF125351, AF125352, AF125353, AF125354, AF125355, AF125356, AF125357, AF125358, AF125359, AF125360, AF125361 Genomic DNA. Translation: AAF37738.1.

IPI

IPI00328228.
IPI00478608.

PIR

S24168.

RefSeq

NP_004753.1. NM_004762.2.
NP_059430.2. NM_017456.2.

UniGene

Hs.191215.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BC9	NMR	-	A	58-248	[»]
4A4P	X-ray	2.00	A/B	63-248	[»]

ProteinModelPortal

Q15438.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15438. 1 interaction.

MINT

MINT-3031502.

STRING

9606.ENSP00000354398.

PTM databases

PhosphoSite

Q15438.

Polymorphism databases

DMDM

2498175.

Proteomic databases

PaxDb

Q15438.

PRIDE

Q15438.

Protocols and materials databases

DNASU

9267.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361101; ENSP00000354398; ENSG00000108669.
ENST00000446868; ENSP00000389095; ENSG00000108669.
ENST00000591455; ENSP00000465665; ENSG00000108669.

GeneID

9267.

KEGG

hsa:9267.

UCSC

uc002jvw.3. human.
uc010wtw.1. human.

Organism-specific databases

CTD

9267.

GeneCards

GC17M076670.

HGNC

HGNC:9501. CYTH1.

MIM

182115. gene.

neXtProt

NX_Q15438.

PharmGKB

PA164718528.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5307.

HOGENOM

HOG000253023.

HOVERGEN

HBG002647.

OMA

SEFSMPE.

OrthoDB

EOG4J1187.

Enzyme and pathway databases

Pathway_Interaction_DB

pi3kcipathway. Class I PI3K signaling events.

Gene expression databases

ArrayExpress

Q15438.

Bgee

Q15438.

CleanEx

HS_CYTH1.

Genevestigator

Q15438.

GermOnline

ENSG00000108669. Homo sapiens.

Family and domain databases

Gene3D

1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.

InterPro

IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000904. Sec7.
IPR023394. SEC7_alpha_orthog.
[Graphical view]

Pfam

PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]

SMART

SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]

SUPFAM

SSF48425. Sec7. 1 hit.

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CYTH1. human.

EvolutionaryTrace

Q15438.

GenomeRNAi

9267.

NextBio

34739.

SOURCE

Search...

Entry information

Entry name

CYH1_HUMAN

Accession

Primary (citable) accession number: Q15438
Secondary accession number(s): A6NFW7, Q9P123, Q9P124

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.