Q15428 (SF3A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Splicing factor 3A subunit 2

Alternative name(s):
SF3a66
Spliceosome-associated protein 62
Short name=SAP 62

Gene names

Name:	SF3A2
Synonyms:	SAP62

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Subunit structure	Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1. Ref.7 Ref.9
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the SF3A2 family. Contains 1 matrin-type zinc finger.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	mRNA 3'-splice site recognition Traceable author statement PubMed 15647371. Source: HGNC
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay Ref.9. Source: UniProtKB nucleoplasm Traceable author statement. Source: Reactome small nuclear ribonucleoprotein complex Traceable author statement Ref.1. Source: ProtInc
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Splicing factor 3A subunit 2		PRO_0000174315
Regions
	Zinc finger	54 – 84	31	Matrin-type
	Compositional bias	217 – 462	246	Pro-rich
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.6
Experimental info
	Sequence conflict	29	1	R → P in AAA60301. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q15428 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: FA46F064A55EA2CE	FASTA	464	49,256
	10 20 30 40 50 60 MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL 70 80 90 100 110 120 TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE KVKVEVKKFV KIGRPGYKVT 130 140 150 160 170 180 KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR FMSAYEQRIE PPDRRWQYLL MAAEPYETIA 190 200 210 220 230 240 FKVPSREIDK AEGKFWTHWN RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG 250 260 270 280 290 300 LPPRPPLPES LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG 310 320 330 340 350 360 VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV HPPAPGVHPP 370 380 390 400 410 420 APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM HPQAPGVHPQ PPGVHPSAPG 430 440 450 460 VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP SEGPGNIPPP PPTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Correspondence between a mammalian spliceosome component and an essential yeast splicing factor." Bennett M., Reed R. Science 262:105-108(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Lymph.
[6]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-10; 151-158 AND 205-213, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[7]	"CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif." Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr. Mol. Cell. Biol. 18:5000-5009(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HTATSF1.
[8]	"Functional association of U2 snRNP with the ATP-independent spliceosomal complex E." Das R., Zhou Z., Reed R. Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
[9]	"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L21990 Genomic DNA. Translation: AAA60301.1. AK314815 mRNA. Translation: BAG37338.1. AC005263 Genomic DNA. Translation: AAC25613.1. CH471139 Genomic DNA. Translation: EAW69398.1. CH471139 Genomic DNA. Translation: EAW69400.1. CH471139 Genomic DNA. Translation: EAW69401.1. CH471139 Genomic DNA. Translation: EAW69402.1. BC004434 mRNA. Translation: AAH04434.1. BC009903 mRNA. Translation: AAH09903.1.
IPI	IPI00017341.
PIR	A47655.
RefSeq	NP_009096.2. NM_007165.4.
UniGene	Hs.115232.
3D structure databases
ProteinModelPortal	Q15428.
SMR	Q15428. Positions 128-216.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15428. 3 interactions.
MINT	MINT-1190499.
STRING	9606.ENSP00000221494.
PTM databases
PhosphoSite	Q15428.
Polymorphism databases
DMDM	20141793.
Proteomic databases
PaxDb	Q15428.
PRIDE	Q15428.
Protocols and materials databases
DNASU	8175.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000221494; ENSP00000221494; ENSG00000104897.
GeneID	8175.
KEGG	hsa:8175.
UCSC	uc002lvg.3. human.
Organism-specific databases
CTD	8175.
GeneCards	GC19P002236.
HGNC	HGNC:10766. SF3A2.
HPA	HPA042843. HPA049439.
MIM	600796. gene.
neXtProt	NX_Q15428.
PharmGKB	PA35684.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5246.
HOVERGEN	HBG060566.
InParanoid	Q15428.
KO	K12826.
OMA	GIHPQAP.
OrthoDB	EOG4P8FK1.
PhylomeDB	Q15428.
Enzyme and pathway databases
Reactome	REACT_1675. mRNA Processing. REACT_71. Gene Expression.
Gene expression databases
Bgee	Q15428.
CleanEx	HS_SF3A2.
Genevestigator	Q15428.
GermOnline	ENSG00000104897. Homo sapiens.
Family and domain databases
InterPro	IPR019134. Cactin_C. IPR015880. Znf_C2H2-like. IPR000690. Znf_C2H2_matrin. IPR003604. Znf_U1. [Graphical view]
SMART	SM01050. CactinC_cactus. 1 hit. SM00355. ZnF_C2H2. 1 hit. SM00451. ZnF_U1. 1 hit. [Graphical view]
PROSITE	PS50171. ZF_MATRIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	8175.
NextBio	30856.
SOURCE	Search...

Entry information

Entry name

SF3A2_HUMAN

Accession

Primary (citable) accession number: Q15428
Secondary accession number(s): B2RBU1, D6W605, O75245

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 27, 2002
Last modified:	May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.