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Protein

Splicing factor 3A subunit 2

Gene

SF3A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • gene expression Source: Reactome
  • mRNA 3'-splice site recognition Source: HGNC
  • mRNA processing Source: HGNC
  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: Reactome
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 2
Alternative name(s):
SF3a66
Spliceosome-associated protein 62
Short name:
SAP 62
Gene namesi
Name:SF3A2
Synonyms:SAP62
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10766. SF3A2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • nucleoplasm Source: Reactome
  • small nuclear ribonucleoprotein complex Source: ProtInc
  • spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35684.

Polymorphism and mutation databases

BioMutaiSF3A2.
DMDMi20141793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Splicing factor 3A subunit 2PRO_0000174315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei10 – 101N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15428.
PaxDbiQ15428.
PRIDEiQ15428.

PTM databases

PhosphoSiteiQ15428.

Expressioni

Gene expression databases

BgeeiQ15428.
CleanExiHS_SF3A2.
ExpressionAtlasiQ15428. baseline and differential.
GenevisibleiQ15428. HS.

Organism-specific databases

HPAiHPA042843.
HPA049439.

Interactioni

Subunit structurei

Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-2462271,EBI-374880
DHX15O431432EBI-2462271,EBI-1237044
SF3A1Q154593EBI-2462271,EBI-1054743

Protein-protein interaction databases

BioGridi113826. 137 interactions.
IntActiQ15428. 107 interactions.
MINTiMINT-1190499.
STRINGi9606.ENSP00000221494.

Structurei

3D structure databases

ProteinModelPortaliQ15428.
SMRiQ15428. Positions 128-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi217 – 462246Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the SF3A2 family.Curated
Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5246.
GeneTreeiENSGT00720000108823.
HOVERGENiHBG060566.
InParanoidiQ15428.
KOiK12826.
OMAiASKFFSY.
OrthoDBiEOG7034J2.
PhylomeDBiQ15428.
TreeFamiTF314370.

Family and domain databases

InterProiIPR019134. Cactin_C.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM01050. CactinC_cactus. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH
60 70 80 90 100
LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE
110 120 130 140 150
KVKVEVKKFV KIGRPGYKVT KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR
160 170 180 190 200
FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN
210 220 230 240 250
RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPES
260 270 280 290 300
LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG
310 320 330 340 350
VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV
360 370 380 390 400
HPPAPGVHPP APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM
410 420 430 440 450
HPQAPGVHPQ PPGVHPSAPG VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP
460
SEGPGNIPPP PPTN
Length:464
Mass (Da):49,256
Last modified:March 27, 2002 - v2
Checksum:iFA46F064A55EA2CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291R → P in AAA60301 (PubMed:8211113).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1.
AK314815 mRNA. Translation: BAG37338.1.
AC005263 Genomic DNA. Translation: AAC25613.1.
CH471139 Genomic DNA. Translation: EAW69398.1.
CH471139 Genomic DNA. Translation: EAW69400.1.
CH471139 Genomic DNA. Translation: EAW69401.1.
CH471139 Genomic DNA. Translation: EAW69402.1.
BC004434 mRNA. Translation: AAH04434.1.
BC009903 mRNA. Translation: AAH09903.1.
CCDSiCCDS12084.1.
PIRiA47655.
RefSeqiNP_009096.2. NM_007165.4.
UniGeneiHs.115232.

Genome annotation databases

EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
GeneIDi8175.
KEGGihsa:8175.
UCSCiuc002lvg.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1.
AK314815 mRNA. Translation: BAG37338.1.
AC005263 Genomic DNA. Translation: AAC25613.1.
CH471139 Genomic DNA. Translation: EAW69398.1.
CH471139 Genomic DNA. Translation: EAW69400.1.
CH471139 Genomic DNA. Translation: EAW69401.1.
CH471139 Genomic DNA. Translation: EAW69402.1.
BC004434 mRNA. Translation: AAH04434.1.
BC009903 mRNA. Translation: AAH09903.1.
CCDSiCCDS12084.1.
PIRiA47655.
RefSeqiNP_009096.2. NM_007165.4.
UniGeneiHs.115232.

3D structure databases

ProteinModelPortaliQ15428.
SMRiQ15428. Positions 128-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113826. 137 interactions.
IntActiQ15428. 107 interactions.
MINTiMINT-1190499.
STRINGi9606.ENSP00000221494.

PTM databases

PhosphoSiteiQ15428.

Polymorphism and mutation databases

BioMutaiSF3A2.
DMDMi20141793.

Proteomic databases

MaxQBiQ15428.
PaxDbiQ15428.
PRIDEiQ15428.

Protocols and materials databases

DNASUi8175.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
GeneIDi8175.
KEGGihsa:8175.
UCSCiuc002lvg.3. human.

Organism-specific databases

CTDi8175.
GeneCardsiGC19P002236.
HGNCiHGNC:10766. SF3A2.
HPAiHPA042843.
HPA049439.
MIMi600796. gene.
neXtProtiNX_Q15428.
PharmGKBiPA35684.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5246.
GeneTreeiENSGT00720000108823.
HOVERGENiHBG060566.
InParanoidiQ15428.
KOiK12826.
OMAiASKFFSY.
OrthoDBiEOG7034J2.
PhylomeDBiQ15428.
TreeFamiTF314370.

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSF3A2. human.
GeneWikiiSF3A2.
GenomeRNAii8175.
NextBioi30856.
PROiQ15428.
SOURCEiSearch...

Gene expression databases

BgeeiQ15428.
CleanExiHS_SF3A2.
ExpressionAtlasiQ15428. baseline and differential.
GenevisibleiQ15428. HS.

Family and domain databases

InterProiIPR019134. Cactin_C.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM01050. CactinC_cactus. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Correspondence between a mammalian spliceosome component and an essential yeast splicing factor."
    Bennett M., Reed R.
    Science 262:105-108(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Lymph.
  6. Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-10; 151-158 AND 205-213, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Ovarian carcinoma.
  7. "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif."
    Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr.
    Mol. Cell. Biol. 18:5000-5009(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HTATSF1.
  8. "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."
    Das R., Zhou Z., Reed R.
    Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
  9. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
    Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
    RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSF3A2_HUMAN
AccessioniPrimary (citable) accession number: Q15428
Secondary accession number(s): B2RBU1, D6W605, O75245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: June 24, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.