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Q15428

- SF3A2_HUMAN

UniProt

Q15428 - SF3A2_HUMAN

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Protein

Splicing factor 3A subunit 2

Gene
SF3A2, SAP62
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typeAdd
BLAST

GO - Molecular functioni

  1. poly(A) RNA binding Source: UniProtKB
  2. protein binding Source: IntAct
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. gene expression Source: Reactome
  2. mRNA 3'-splice site recognition Source: HGNC
  3. mRNA processing Source: HGNC
  4. mRNA splicing, via spliceosome Source: UniProtKB
  5. RNA splicing Source: Reactome
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_467. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 2
Alternative name(s):
SF3a66
Spliceosome-associated protein 62
Short name:
SAP 62
Gene namesi
Name:SF3A2
Synonyms:SAP62
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:10766. SF3A2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. catalytic step 2 spliceosome Source: UniProtKB
  2. nucleoplasm Source: Reactome
  3. small nuclear ribonucleoprotein complex Source: ProtInc
  4. spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35684.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Splicing factor 3A subunit 2PRO_0000174315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei10 – 101N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15428.
PaxDbiQ15428.
PRIDEiQ15428.

PTM databases

PhosphoSiteiQ15428.

Expressioni

Gene expression databases

ArrayExpressiQ15428.
BgeeiQ15428.
CleanExiHS_SF3A2.
GenevestigatoriQ15428.

Organism-specific databases

HPAiHPA042843.
HPA049439.

Interactioni

Subunit structurei

Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-2462271,EBI-374880
DHX15O431432EBI-2462271,EBI-1237044
SF3A1Q154593EBI-2462271,EBI-1054743

Protein-protein interaction databases

BioGridi113826. 135 interactions.
IntActiQ15428. 107 interactions.
MINTiMINT-1190499.
STRINGi9606.ENSP00000221494.

Structurei

3D structure databases

ProteinModelPortaliQ15428.
SMRiQ15428. Positions 128-216.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi217 – 462246Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the SF3A2 family.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri54 – 8431Matrin-typeAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5246.
HOVERGENiHBG060566.
InParanoidiQ15428.
KOiK12826.
OMAiASKFFSY.
OrthoDBiEOG7034J2.
PhylomeDBiQ15428.
TreeFamiTF314370.

Family and domain databases

InterProiIPR019134. Cactin_C.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
SMARTiSM01050. CactinC_cactus. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15428-1 [UniParc]FASTAAdd to Basket

« Hide

MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH    50
LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE 100
KVKVEVKKFV KIGRPGYKVT KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR 150
FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN 200
RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPES 250
LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG 300
VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV 350
HPPAPGVHPP APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM 400
HPQAPGVHPQ PPGVHPSAPG VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP 450
SEGPGNIPPP PPTN 464
Length:464
Mass (Da):49,256
Last modified:March 27, 2002 - v2
Checksum:iFA46F064A55EA2CE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291R → P in AAA60301. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1.
AK314815 mRNA. Translation: BAG37338.1.
AC005263 Genomic DNA. Translation: AAC25613.1.
CH471139 Genomic DNA. Translation: EAW69398.1.
CH471139 Genomic DNA. Translation: EAW69400.1.
CH471139 Genomic DNA. Translation: EAW69401.1.
CH471139 Genomic DNA. Translation: EAW69402.1.
BC004434 mRNA. Translation: AAH04434.1.
BC009903 mRNA. Translation: AAH09903.1.
CCDSiCCDS12084.1.
PIRiA47655.
RefSeqiNP_009096.2. NM_007165.4.
UniGeneiHs.115232.

Genome annotation databases

EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
GeneIDi8175.
KEGGihsa:8175.
UCSCiuc002lvg.3. human.

Polymorphism databases

DMDMi20141793.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1 .
AK314815 mRNA. Translation: BAG37338.1 .
AC005263 Genomic DNA. Translation: AAC25613.1 .
CH471139 Genomic DNA. Translation: EAW69398.1 .
CH471139 Genomic DNA. Translation: EAW69400.1 .
CH471139 Genomic DNA. Translation: EAW69401.1 .
CH471139 Genomic DNA. Translation: EAW69402.1 .
BC004434 mRNA. Translation: AAH04434.1 .
BC009903 mRNA. Translation: AAH09903.1 .
CCDSi CCDS12084.1.
PIRi A47655.
RefSeqi NP_009096.2. NM_007165.4.
UniGenei Hs.115232.

3D structure databases

ProteinModelPortali Q15428.
SMRi Q15428. Positions 128-216.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113826. 135 interactions.
IntActi Q15428. 107 interactions.
MINTi MINT-1190499.
STRINGi 9606.ENSP00000221494.

PTM databases

PhosphoSitei Q15428.

Polymorphism databases

DMDMi 20141793.

Proteomic databases

MaxQBi Q15428.
PaxDbi Q15428.
PRIDEi Q15428.

Protocols and materials databases

DNASUi 8175.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000221494 ; ENSP00000221494 ; ENSG00000104897 .
GeneIDi 8175.
KEGGi hsa:8175.
UCSCi uc002lvg.3. human.

Organism-specific databases

CTDi 8175.
GeneCardsi GC19P002236.
HGNCi HGNC:10766. SF3A2.
HPAi HPA042843.
HPA049439.
MIMi 600796. gene.
neXtProti NX_Q15428.
PharmGKBi PA35684.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5246.
HOVERGENi HBG060566.
InParanoidi Q15428.
KOi K12826.
OMAi ASKFFSY.
OrthoDBi EOG7034J2.
PhylomeDBi Q15428.
TreeFami TF314370.

Enzyme and pathway databases

Reactomei REACT_467. mRNA Splicing - Major Pathway.

Miscellaneous databases

GeneWikii SF3A2.
GenomeRNAii 8175.
NextBioi 30856.
PROi Q15428.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q15428.
Bgeei Q15428.
CleanExi HS_SF3A2.
Genevestigatori Q15428.

Family and domain databases

InterProi IPR019134. Cactin_C.
IPR015880. Znf_C2H2-like.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view ]
SMARTi SM01050. CactinC_cactus. 1 hit.
SM00355. ZnF_C2H2. 1 hit.
SM00451. ZnF_U1. 1 hit.
[Graphical view ]
PROSITEi PS50171. ZF_MATRIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Correspondence between a mammalian spliceosome component and an essential yeast splicing factor."
    Bennett M., Reed R.
    Science 262:105-108(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Lymph.
  6. Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.
    Submitted (DEC-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 1-10; 151-158 AND 205-213, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Ovarian carcinoma.
  7. "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif."
    Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr.
    Mol. Cell. Biol. 18:5000-5009(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HTATSF1.
  8. "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."
    Das R., Zhou Z., Reed R.
    Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
  9. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
    Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
    RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSF3A2_HUMAN
AccessioniPrimary (citable) accession number: Q15428
Secondary accession number(s): B2RBU1, D6W605, O75245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: September 3, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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