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Q15428

- SF3A2_HUMAN

UniProt

Q15428 - SF3A2_HUMAN

Protein

Splicing factor 3A subunit 2

Gene

SF3A2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. poly(A) RNA binding Source: UniProtKB
    2. protein binding Source: IntAct
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. gene expression Source: Reactome
    2. mRNA 3'-splice site recognition Source: HGNC
    3. mRNA processing Source: HGNC
    4. mRNA splicing, via spliceosome Source: UniProtKB
    5. RNA splicing Source: Reactome

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_467. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Splicing factor 3A subunit 2
    Alternative name(s):
    SF3a66
    Spliceosome-associated protein 62
    Short name:
    SAP 62
    Gene namesi
    Name:SF3A2
    Synonyms:SAP62
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:10766. SF3A2.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. catalytic step 2 spliceosome Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. small nuclear ribonucleoprotein complex Source: ProtInc
    4. spliceosomal complex Source: HGNC

    Keywords - Cellular componenti

    Nucleus, Spliceosome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35684.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Splicing factor 3A subunit 2PRO_0000174315Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei10 – 101N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ15428.
    PaxDbiQ15428.
    PRIDEiQ15428.

    PTM databases

    PhosphoSiteiQ15428.

    Expressioni

    Gene expression databases

    ArrayExpressiQ15428.
    BgeeiQ15428.
    CleanExiHS_SF3A2.
    GenevestigatoriQ15428.

    Organism-specific databases

    HPAiHPA042843.
    HPA049439.

    Interactioni

    Subunit structurei

    Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC5LQ994592EBI-2462271,EBI-374880
    DHX15O431432EBI-2462271,EBI-1237044
    SF3A1Q154593EBI-2462271,EBI-1054743

    Protein-protein interaction databases

    BioGridi113826. 135 interactions.
    IntActiQ15428. 107 interactions.
    MINTiMINT-1190499.
    STRINGi9606.ENSP00000221494.

    Structurei

    3D structure databases

    ProteinModelPortaliQ15428.
    SMRiQ15428. Positions 128-216.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi217 – 462246Pro-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the SF3A2 family.Curated
    Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri54 – 8431Matrin-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5246.
    HOVERGENiHBG060566.
    InParanoidiQ15428.
    KOiK12826.
    OMAiASKFFSY.
    OrthoDBiEOG7034J2.
    PhylomeDBiQ15428.
    TreeFamiTF314370.

    Family and domain databases

    InterProiIPR019134. Cactin_C.
    IPR015880. Znf_C2H2-like.
    IPR000690. Znf_C2H2_matrin.
    IPR003604. Znf_U1.
    [Graphical view]
    SMARTiSM01050. CactinC_cactus. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    SM00451. ZnF_U1. 1 hit.
    [Graphical view]
    PROSITEiPS50171. ZF_MATRIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q15428-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH    50
    LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE 100
    KVKVEVKKFV KIGRPGYKVT KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR 150
    FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN 200
    RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPES 250
    LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG 300
    VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV 350
    HPPAPGVHPP APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM 400
    HPQAPGVHPQ PPGVHPSAPG VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP 450
    SEGPGNIPPP PPTN 464
    Length:464
    Mass (Da):49,256
    Last modified:March 27, 2002 - v2
    Checksum:iFA46F064A55EA2CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 291R → P in AAA60301. (PubMed:8211113)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21990 Genomic DNA. Translation: AAA60301.1.
    AK314815 mRNA. Translation: BAG37338.1.
    AC005263 Genomic DNA. Translation: AAC25613.1.
    CH471139 Genomic DNA. Translation: EAW69398.1.
    CH471139 Genomic DNA. Translation: EAW69400.1.
    CH471139 Genomic DNA. Translation: EAW69401.1.
    CH471139 Genomic DNA. Translation: EAW69402.1.
    BC004434 mRNA. Translation: AAH04434.1.
    BC009903 mRNA. Translation: AAH09903.1.
    CCDSiCCDS12084.1.
    PIRiA47655.
    RefSeqiNP_009096.2. NM_007165.4.
    UniGeneiHs.115232.

    Genome annotation databases

    EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
    GeneIDi8175.
    KEGGihsa:8175.
    UCSCiuc002lvg.3. human.

    Polymorphism databases

    DMDMi20141793.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21990 Genomic DNA. Translation: AAA60301.1 .
    AK314815 mRNA. Translation: BAG37338.1 .
    AC005263 Genomic DNA. Translation: AAC25613.1 .
    CH471139 Genomic DNA. Translation: EAW69398.1 .
    CH471139 Genomic DNA. Translation: EAW69400.1 .
    CH471139 Genomic DNA. Translation: EAW69401.1 .
    CH471139 Genomic DNA. Translation: EAW69402.1 .
    BC004434 mRNA. Translation: AAH04434.1 .
    BC009903 mRNA. Translation: AAH09903.1 .
    CCDSi CCDS12084.1.
    PIRi A47655.
    RefSeqi NP_009096.2. NM_007165.4.
    UniGenei Hs.115232.

    3D structure databases

    ProteinModelPortali Q15428.
    SMRi Q15428. Positions 128-216.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113826. 135 interactions.
    IntActi Q15428. 107 interactions.
    MINTi MINT-1190499.
    STRINGi 9606.ENSP00000221494.

    PTM databases

    PhosphoSitei Q15428.

    Polymorphism databases

    DMDMi 20141793.

    Proteomic databases

    MaxQBi Q15428.
    PaxDbi Q15428.
    PRIDEi Q15428.

    Protocols and materials databases

    DNASUi 8175.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000221494 ; ENSP00000221494 ; ENSG00000104897 .
    GeneIDi 8175.
    KEGGi hsa:8175.
    UCSCi uc002lvg.3. human.

    Organism-specific databases

    CTDi 8175.
    GeneCardsi GC19P002236.
    HGNCi HGNC:10766. SF3A2.
    HPAi HPA042843.
    HPA049439.
    MIMi 600796. gene.
    neXtProti NX_Q15428.
    PharmGKBi PA35684.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5246.
    HOVERGENi HBG060566.
    InParanoidi Q15428.
    KOi K12826.
    OMAi ASKFFSY.
    OrthoDBi EOG7034J2.
    PhylomeDBi Q15428.
    TreeFami TF314370.

    Enzyme and pathway databases

    Reactomei REACT_467. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    GeneWikii SF3A2.
    GenomeRNAii 8175.
    NextBioi 30856.
    PROi Q15428.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q15428.
    Bgeei Q15428.
    CleanExi HS_SF3A2.
    Genevestigatori Q15428.

    Family and domain databases

    InterProi IPR019134. Cactin_C.
    IPR015880. Znf_C2H2-like.
    IPR000690. Znf_C2H2_matrin.
    IPR003604. Znf_U1.
    [Graphical view ]
    SMARTi SM01050. CactinC_cactus. 1 hit.
    SM00355. ZnF_C2H2. 1 hit.
    SM00451. ZnF_U1. 1 hit.
    [Graphical view ]
    PROSITEi PS50171. ZF_MATRIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Correspondence between a mammalian spliceosome component and an essential yeast splicing factor."
      Bennett M., Reed R.
      Science 262:105-108(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Umbilical cord blood.
    3. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung and Lymph.
    6. Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.
      Submitted (DEC-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-10; 151-158 AND 205-213, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Ovarian carcinoma.
    7. "CUS2, a yeast homolog of human Tat-SF1, rescues function of misfolded U2 through an unusual RNA recognition motif."
      Yan D., Perriman R., Igel H., Howe K.J., Neville M., Ares M. Jr.
      Mol. Cell. Biol. 18:5000-5009(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HTATSF1.
    8. "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E."
      Das R., Zhou Z., Reed R.
      Mol. Cell 5:779-787(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF THE SPLICEOSOME.
    9. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
      Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
      RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSF3A2_HUMAN
    AccessioniPrimary (citable) accession number: Q15428
    Secondary accession number(s): B2RBU1, D6W605, O75245
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3