Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Splicing factor 3A subunit 2

Gene

SF3A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 84Matrin-typePROSITE-ProRule annotationAdd BLAST31

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mRNA 3'-splice site recognition Source: HGNC
  • mRNA processing Source: HGNC
  • mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of neuron projection development Source: Ensembl
  • RNA splicing Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104897-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3A subunit 2
Alternative name(s):
SF3a66
Spliceosome-associated protein 62
Short name:
SAP 62
Gene namesi
Name:SF3A2
Synonyms:SAP62
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10766. SF3A2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • nuclear speck Source: Ensembl
  • nucleoplasm Source: Reactome
  • small nuclear ribonucleoprotein complex Source: ProtInc
  • spliceosomal complex Source: HGNC
  • U2 snRNP Source: GO_Central
  • U2-type prespliceosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8175.
OpenTargetsiENSG00000104897.
PharmGKBiPA35684.

Polymorphism and mutation databases

BioMutaiSF3A2.
DMDMi20141793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743151 – 464Splicing factor 3A subunit 2Add BLAST464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei10N6-acetyllysineBy similarity1
Modified residuei153PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15428.
MaxQBiQ15428.
PaxDbiQ15428.
PeptideAtlasiQ15428.
PRIDEiQ15428.

PTM databases

iPTMnetiQ15428.
PhosphoSitePlusiQ15428.

Expressioni

Gene expression databases

BgeeiENSG00000104897.
CleanExiHS_SF3A2.
ExpressionAtlasiQ15428. baseline and differential.
GenevisibleiQ15428. HS.

Organism-specific databases

HPAiHPA042843.
HPA049439.

Interactioni

Subunit structurei

Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5LQ994592EBI-2462271,EBI-374880
DHX15O431432EBI-2462271,EBI-1237044
SF1Q156372EBI-2462271,EBI-744603
SF3A1Q154593EBI-2462271,EBI-1054743

Protein-protein interaction databases

BioGridi113826. 151 interactors.
DIPiDIP-42521N.
IntActiQ15428. 117 interactors.
MINTiMINT-1190499.
STRINGi9606.ENSP00000221494.

Structurei

3D structure databases

ProteinModelPortaliQ15428.
SMRiQ15428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi217 – 462Pro-richAdd BLAST246

Sequence similaritiesi

Belongs to the SF3A2 family.Curated
Contains 1 matrin-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri54 – 84Matrin-typePROSITE-ProRule annotationAdd BLAST31

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0227. Eukaryota.
COG5246. LUCA.
GeneTreeiENSGT00720000108823.
HOVERGENiHBG060566.
InParanoidiQ15428.
KOiK12826.
OMAiGRPGYKX.
OrthoDBiEOG091G0IZX.
PhylomeDBiQ15428.
TreeFamiTF314370.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH
60 70 80 90 100
LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKEA KEAPAQPAPE
110 120 130 140 150
KVKVEVKKFV KIGRPGYKVT KQRDSEMGQQ SLLFQIDYPE IAEGIMPRHR
160 170 180 190 200
FMSAYEQRIE PPDRRWQYLL MAAEPYETIA FKVPSREIDK AEGKFWTHWN
210 220 230 240 250
RETKQFFLQF HFKMEKPPAP PSLPAGPPGV KRPPPPLMNG LPPRPPLPES
260 270 280 290 300
LPPPPPGGLP LPPMPPTGPA PSGPPGPPQL PPPAPGVHPP APVVHPPASG
310 320 330 340 350
VHPPAPGVHP PAPGVHPPAP GVHPPTSGVH PPAPGVHPPA PGVHPPAPGV
360 370 380 390 400
HPPAPGVHPP APGVHPPPSA GVHPQAPGVH PAAPAVHPQA PGVHPPAPGM
410 420 430 440 450
HPQAPGVHPQ PPGVHPSAPG VHPQPPGVHP SNPGVHPPTP MPPMLRPPLP
460
SEGPGNIPPP PPTN
Length:464
Mass (Da):49,256
Last modified:March 27, 2002 - v2
Checksum:iFA46F064A55EA2CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29R → P in AAA60301 (PubMed:8211113).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1.
AK314815 mRNA. Translation: BAG37338.1.
AC005263 Genomic DNA. Translation: AAC25613.1.
CH471139 Genomic DNA. Translation: EAW69398.1.
CH471139 Genomic DNA. Translation: EAW69400.1.
CH471139 Genomic DNA. Translation: EAW69401.1.
CH471139 Genomic DNA. Translation: EAW69402.1.
BC004434 mRNA. Translation: AAH04434.1.
BC009903 mRNA. Translation: AAH09903.1.
CCDSiCCDS12084.1.
PIRiA47655.
RefSeqiNP_009096.2. NM_007165.4.
UniGeneiHs.115232.

Genome annotation databases

EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
GeneIDi8175.
KEGGihsa:8175.
UCSCiuc002lvg.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21990 Genomic DNA. Translation: AAA60301.1.
AK314815 mRNA. Translation: BAG37338.1.
AC005263 Genomic DNA. Translation: AAC25613.1.
CH471139 Genomic DNA. Translation: EAW69398.1.
CH471139 Genomic DNA. Translation: EAW69400.1.
CH471139 Genomic DNA. Translation: EAW69401.1.
CH471139 Genomic DNA. Translation: EAW69402.1.
BC004434 mRNA. Translation: AAH04434.1.
BC009903 mRNA. Translation: AAH09903.1.
CCDSiCCDS12084.1.
PIRiA47655.
RefSeqiNP_009096.2. NM_007165.4.
UniGeneiHs.115232.

3D structure databases

ProteinModelPortaliQ15428.
SMRiQ15428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113826. 151 interactors.
DIPiDIP-42521N.
IntActiQ15428. 117 interactors.
MINTiMINT-1190499.
STRINGi9606.ENSP00000221494.

PTM databases

iPTMnetiQ15428.
PhosphoSitePlusiQ15428.

Polymorphism and mutation databases

BioMutaiSF3A2.
DMDMi20141793.

Proteomic databases

EPDiQ15428.
MaxQBiQ15428.
PaxDbiQ15428.
PeptideAtlasiQ15428.
PRIDEiQ15428.

Protocols and materials databases

DNASUi8175.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221494; ENSP00000221494; ENSG00000104897.
GeneIDi8175.
KEGGihsa:8175.
UCSCiuc002lvg.4. human.

Organism-specific databases

CTDi8175.
DisGeNETi8175.
GeneCardsiSF3A2.
HGNCiHGNC:10766. SF3A2.
HPAiHPA042843.
HPA049439.
MIMi600796. gene.
neXtProtiNX_Q15428.
OpenTargetsiENSG00000104897.
PharmGKBiPA35684.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0227. Eukaryota.
COG5246. LUCA.
GeneTreeiENSGT00720000108823.
HOVERGENiHBG060566.
InParanoidiQ15428.
KOiK12826.
OMAiGRPGYKX.
OrthoDBiEOG091G0IZX.
PhylomeDBiQ15428.
TreeFamiTF314370.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000104897-MONOMER.
ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiSF3A2. human.
GeneWikiiSF3A2.
GenomeRNAii8175.
PROiQ15428.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104897.
CleanExiHS_SF3A2.
ExpressionAtlasiQ15428. baseline and differential.
GenevisibleiQ15428. HS.

Family and domain databases

InterProiIPR031781. SF3A2_dom.
IPR000690. Znf_C2H2_matrin.
IPR003604. Znf_U1.
[Graphical view]
PfamiPF16835. SF3A2. 1 hit.
[Graphical view]
SMARTiSM00451. ZnF_U1. 1 hit.
[Graphical view]
PROSITEiPS50171. ZF_MATRIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSF3A2_HUMAN
AccessioniPrimary (citable) accession number: Q15428
Secondary accession number(s): B2RBU1, D6W605, O75245
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 27, 2002
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.