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Protein

Scaffold attachment factor B1

Gene

SAFB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity). Can inhibit cell proliferation.By similarity

GO - Molecular functioni

  • chromatin binding Source: Ensembl
  • core promoter binding Source: Ensembl
  • double-stranded DNA binding Source: ProtInc
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B1
Short name:
SAF-B
Short name:
SAF-B1
Alternative name(s):
HSP27 estrogen response element-TATA box-binding protein
Short name:
HSP27 ERE-TATA-binding protein
Gene namesi
Name:SAFB
Synonyms:HAP, HET, SAFB1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:10520. SAFB.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34928.

Polymorphism and mutation databases

BioMutaiSAFB.
DMDMi116242782.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 915914Scaffold attachment factor B1PRO_0000081905Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources1 Publication
Modified residuei24 – 241PhosphoserineBy similarity
Modified residuei55 – 551PhosphoserineCombined sources
Modified residuei79 – 791PhosphoserineCombined sources
Cross-linki172 – 172Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei188 – 1881PhosphothreonineCombined sources
Modified residuei195 – 1951PhosphoserineCombined sources
Modified residuei197 – 1971PhosphoserineCombined sources
Modified residuei209 – 2091PhosphoserineBy similarity
Cross-linki231 – 231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki294 – 294Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei383 – 3831PhosphoserineCombined sources
Modified residuei384 – 3841PhosphoserineCombined sources
Modified residuei415 – 4151PhosphoserineCombined sources
Cross-linki483 – 483Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki543 – 543Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki578 – 578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)Combined sources
Cross-linki578 – 578Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei580 – 5801PhosphoserineCombined sources
Modified residuei582 – 5821PhosphoserineCombined sources
Modified residuei601 – 6011PhosphoserineCombined sources
Modified residuei604 – 6041PhosphoserineCombined sources
Modified residuei607 – 6071N6-acetyllysineCombined sources

Post-translational modificationi

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15424.
MaxQBiQ15424.
PaxDbiQ15424.
PeptideAtlasiQ15424.
PRIDEiQ15424.

PTM databases

iPTMnetiQ15424.
PhosphoSiteiQ15424.
SwissPalmiQ15424.

Expressioni

Tissue specificityi

Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.

Gene expression databases

BgeeiENSG00000160633.
CleanExiHS_SAFB.
ExpressionAtlasiQ15424. baseline and differential.
GenevisibleiQ15424. HS.

Organism-specific databases

HPAiCAB001969.
HPA016832.
HPA020076.

Interactioni

Subunit structurei

Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with isoform 1 and isoform 2 of SRPK1 and inhibits its activity. Interacts with RBMX (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MAP1LC3BQ9GZQ83EBI-348298,EBI-373144
SAFB2Q141513EBI-348298,EBI-352869
Srek1Q9JKL73EBI-348298,EBI-6452221From a different organism.
SRPK1Q96SB4-22EBI-348298,EBI-5773439
SRPK1Q96SB4-32EBI-348298,EBI-7160164
TP53P046375EBI-348298,EBI-366083

Protein-protein interaction databases

BioGridi112201. 89 interactions.
IntActiQ15424. 30 interactions.
MINTiMINT-1033560.
STRINGi9606.ENSP00000292123.

Structurei

3D structure databases

ProteinModelPortaliQ15424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 6535SAPPROSITE-ProRule annotationAdd
BLAST
Domaini406 – 48479RRMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni528 – 792265Interaction with POLR2AAdd
BLAST
Regioni599 – 915317Interaction with SAFB2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi599 – 61618Nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi612 – 831220Arg-richAdd
BLAST
Compositional biasi625 – 70581Glu-richAdd
BLAST
Compositional biasi785 – 903119Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4661. Eukaryota.
ENOG4111F1G. LUCA.
GeneTreeiENSGT00730000110777.
HOGENOMiHOG000092533.
HOVERGENiHBG078408.
InParanoidiQ15424.
OMAiKPACRRE.
OrthoDBiEOG091G0GPT.
PhylomeDBiQ15424.
TreeFamiTF325240.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15424-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAETLSGLGD SGAAGAAALS SASSETGTRR LSDLRVIDLR AELRKRNVDS
60 70 80 90 100
SGNKSVLMER LKKAIEDEGG NPDEIEITSE GNKKTSKRSS KGRKPEEEGV
110 120 130 140 150
EDNGLEENSG DGQEDVETSL ENLQDIDIMD ISVLDEAEID NGSVADCVED
160 170 180 190 200
DDADNLQESL SDSRELVEGE MKELPEQLQE HAIEDKETIN NLDTSSSDFT
210 220 230 240 250
ILQEIEEPSL EPENEKILDI LGETCKSEPV KEESSELEQP FAQDTSSVGP
260 270 280 290 300
DRKLAEEEDL FDSAHPEEGD LDLASESTAH AQSSKADSLL AVVKREPAEQ
310 320 330 340 350
PGDGERTDCE PVGLEPAVEQ SSAASELAEA SSEELAEAPT EAPSPEARDS
360 370 380 390 400
KEDGRKFDFD ACNEVPPAPK ESSTSEGADQ KMSSPEDDSD TKRLSKEEKG
410 420 430 440 450
RSSCGRNFWV SGLSSTTRAT DLKNLFSKYG KVVGAKVVTN ARSPGARCYG
460 470 480 490 500
FVTMSTAEEA TKCINHLHKT ELHGKMISVE KAKNEPVGKK TSDKRDSDGK
510 520 530 540 550
KEKSSNSDRS TNLKRDDKCD RKDDAKKGDD GSGEKSKDQD DQKPGPSERS
560 570 580 590 600
RATKSGSRGT ERTVVMDKSK GVPVISVKTS GSKERASKSQ DRKSASREKR
610 620 630 640 650
SVVSFDKVKE PRKSRDSESH SRVRERSERE QRMQAQWERE ERERLEIARE
660 670 680 690 700
RLAFQRQRLE RERMERERLE RERMHVEHER RREQERIHRE REELRRQQEL
710 720 730 740 750
RYEQERRPAV RRPYDLDRRD DAYWPEAKRA ALDERYHSDF NRQDRFHDFD
760 770 780 790 800
HRDRGRYPDH SVDRREGSRS MMGEREGQHY PERHGGPERH GRDSRDGWGG
810 820 830 840 850
YGSDKRMSEG RGLPPPPRRD WGDHGRREDD RSWQGTADGG MMDRDHKRWQ
860 870 880 890 900
GGERSMSGHS GPGHMMNRGG MSGRGSFAPG GASRGHPIPH GGMQGGFGGQ
910
SRGSRPSDAR FTRRY
Length:915
Mass (Da):102,642
Last modified:October 17, 2006 - v4
Checksum:iE865940BC7783C4A
GO
Isoform 2 (identifier: Q15424-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-182: Missing.
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:848
Mass (Da):95,181
Checksum:i681FC59B003DBAD5
GO
Isoform 3 (identifier: Q15424-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:917
Mass (Da):102,855
Checksum:i9DB77C0D393EA6BB
GO
Isoform 4 (identifier: Q15424-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-621: Missing.
     817-817: P → PRG

Note: No experimental confirmation available.
Show »
Length:916
Mass (Da):102,768
Checksum:i6A3B946D0D5A0733
GO

Sequence cautioni

The sequence BAD92017 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161A → P in AAC00056 (PubMed:9328833).Curated
Sequence conflicti42 – 432EL → DV in AAC00056 (PubMed:9328833).Curated
Sequence conflicti197 – 1971S → P in BAH12852 (PubMed:14702039).Curated
Sequence conflicti463 – 4631C → Y in BAH12852 (PubMed:14702039).Curated
Sequence conflicti692 – 6921E → V in BAH12852 (PubMed:14702039).Curated
Sequence conflicti731 – 7311A → G in AAC00056 (PubMed:9328833).Curated
Sequence conflicti731 – 7311A → G in AAC18697 (PubMed:8600450).Curated
Sequence conflicti744 – 7441D → E in AAC00056 (PubMed:9328833).Curated
Sequence conflicti744 – 7441D → E in AAC18697 (PubMed:8600450).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei114 – 18269Missing in isoform 2. 1 PublicationVSP_045838Add
BLAST
Alternative sequencei621 – 6211Missing in isoform 4. 2 PublicationsVSP_046902
Alternative sequencei817 – 8171P → PRG in isoform 2, isoform 3 and isoform 4. 3 PublicationsVSP_045839

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72355 mRNA. Translation: AAC00056.1.
AK298707 mRNA. Translation: BAH12852.1.
AC004611 Genomic DNA. Translation: AAC14667.1.
AC011499 Genomic DNA. No translation available.
AC134303 Genomic DNA. No translation available.
BC126219 mRNA. Translation: AAI26220.1.
BC143937 mRNA. Translation: AAI43938.1.
BC143939 mRNA. Translation: AAI43940.1.
AB208780 mRNA. Translation: BAD92017.1. Different initiation.
L43631 mRNA. Translation: AAC18697.1.
CCDSiCCDS12142.1. [Q15424-1]
CCDS56077.1. [Q15424-2]
CCDS59339.1. [Q15424-3]
CCDS59340.1. [Q15424-4]
PIRiS64732.
RefSeqiNP_001188267.1. NM_001201338.1. [Q15424-3]
NP_001188268.1. NM_001201339.1. [Q15424-4]
NP_001188269.1. NM_001201340.1. [Q15424-2]
NP_001307500.1. NM_001320571.1.
NP_001307501.1. NM_001320572.1.
NP_002958.2. NM_002967.3. [Q15424-1]
UniGeneiHs.728802.

Genome annotation databases

EnsembliENST00000292123; ENSP00000292123; ENSG00000160633. [Q15424-1]
ENST00000454510; ENSP00000415895; ENSG00000160633. [Q15424-2]
ENST00000588852; ENSP00000467423; ENSG00000160633. [Q15424-3]
ENST00000592224; ENSP00000464840; ENSG00000160633. [Q15424-4]
GeneIDi6294.
KEGGihsa:6294.
UCSCiuc002mce.5. human. [Q15424-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72355 mRNA. Translation: AAC00056.1.
AK298707 mRNA. Translation: BAH12852.1.
AC004611 Genomic DNA. Translation: AAC14667.1.
AC011499 Genomic DNA. No translation available.
AC134303 Genomic DNA. No translation available.
BC126219 mRNA. Translation: AAI26220.1.
BC143937 mRNA. Translation: AAI43938.1.
BC143939 mRNA. Translation: AAI43940.1.
AB208780 mRNA. Translation: BAD92017.1. Different initiation.
L43631 mRNA. Translation: AAC18697.1.
CCDSiCCDS12142.1. [Q15424-1]
CCDS56077.1. [Q15424-2]
CCDS59339.1. [Q15424-3]
CCDS59340.1. [Q15424-4]
PIRiS64732.
RefSeqiNP_001188267.1. NM_001201338.1. [Q15424-3]
NP_001188268.1. NM_001201339.1. [Q15424-4]
NP_001188269.1. NM_001201340.1. [Q15424-2]
NP_001307500.1. NM_001320571.1.
NP_001307501.1. NM_001320572.1.
NP_002958.2. NM_002967.3. [Q15424-1]
UniGeneiHs.728802.

3D structure databases

ProteinModelPortaliQ15424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112201. 89 interactions.
IntActiQ15424. 30 interactions.
MINTiMINT-1033560.
STRINGi9606.ENSP00000292123.

PTM databases

iPTMnetiQ15424.
PhosphoSiteiQ15424.
SwissPalmiQ15424.

Polymorphism and mutation databases

BioMutaiSAFB.
DMDMi116242782.

Proteomic databases

EPDiQ15424.
MaxQBiQ15424.
PaxDbiQ15424.
PeptideAtlasiQ15424.
PRIDEiQ15424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292123; ENSP00000292123; ENSG00000160633. [Q15424-1]
ENST00000454510; ENSP00000415895; ENSG00000160633. [Q15424-2]
ENST00000588852; ENSP00000467423; ENSG00000160633. [Q15424-3]
ENST00000592224; ENSP00000464840; ENSG00000160633. [Q15424-4]
GeneIDi6294.
KEGGihsa:6294.
UCSCiuc002mce.5. human. [Q15424-1]

Organism-specific databases

CTDi6294.
GeneCardsiSAFB.
H-InvDBHIX0014682.
HGNCiHGNC:10520. SAFB.
HPAiCAB001969.
HPA016832.
HPA020076.
MIMi602895. gene.
neXtProtiNX_Q15424.
PharmGKBiPA34928.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4661. Eukaryota.
ENOG4111F1G. LUCA.
GeneTreeiENSGT00730000110777.
HOGENOMiHOG000092533.
HOVERGENiHBG078408.
InParanoidiQ15424.
OMAiKPACRRE.
OrthoDBiEOG091G0GPT.
PhylomeDBiQ15424.
TreeFamiTF325240.

Miscellaneous databases

ChiTaRSiSAFB. human.
GeneWikiiSAFB.
GenomeRNAii6294.
PROiQ15424.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160633.
CleanExiHS_SAFB.
ExpressionAtlasiQ15424. baseline and differential.
GenevisibleiQ15424. HS.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAFB1_HUMAN
AccessioniPrimary (citable) accession number: Q15424
Secondary accession number(s): A0AV56
, B7Z5B6, B7ZLP6, F5H0H3, O60406, Q59HH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: October 17, 2006
Last modified: September 7, 2016
This is version 162 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.