Reviewed,
UniProtKB/Swiss-Prot Q15424 (SAFB1_HUMAN)
Last modified
June 16, 2009.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Scaffold attachment factor B1 Alternative name(s): SAB-B1 SAF-B HSP27 estrogen response element-TATA box-binding protein Short name=HSP27 ERE-TATA-binding protein | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 915 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing By similarity. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. |
| Subunit structure | Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 By similarity. |
| Subcellular location | |
| Tissue specificity | Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines. |
| Sequence similarities | Contains 1 RRM (RNA recognition motif) domain. Contains 1 SAP domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Ligand | DNA-binding RNA-binding |
| Molecular function | Repressor |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | establishment or maintenance of chromatin architecture Traceable author statement. Source: ProtInc regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Traceable author statement. Source: ProtInc |
| Molecular function | RNA binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded DNA bindingTraceable author statement. Source: ProtInc nucleotide bindingInferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 915 | 915 | Scaffold attachment factor B1 | PRO_0000081905 | |||||
Regions | |||||||||
| Domain | 31 – 65 | 35 | SAP | ||||||
| Domain | 406 – 484 | 79 | RRM | ||||||
| Region | 528 – 792 | 265 | Interaction with POLR2A | ||||||
| Region | 599 – 915 | 317 | Interaction with SAFB2 | ||||||
| Motif | 599 – 616 | 18 | Nuclear localization signal Potential | ||||||
| Compositional bias | 612 – 831 | 220 | Arg-rich | ||||||
| Compositional bias | 625 – 705 | 81 | Glu-rich | ||||||
| Compositional bias | 785 – 903 | 119 | Gly-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 55 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 288 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 344 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 373 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 383 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 384 | 1 | Phosphoserine Ref.11 Ref.7 | ||||||
| Modified residue | 555 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 557 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 589 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 601 | 1 | Phosphoserine Ref.11 Ref.8 | ||||||
| Modified residue | 604 | 1 | Phosphoserine Ref.11 Ref.8 Ref.7 Ref.9 | ||||||
| Modified residue | 808 | 1 | Phosphoserine Ref.7 | ||||||
Experimental info | |||||||||
| Sequence conflict | 16 | 1 | A → P in AAC00056. Ref.1 | ||||||
| Sequence conflict | 42 – 43 | 2 | EL → DV in AAC00056. Ref.1 | ||||||
| Sequence conflict | 621 | 1 | Missing in BAD92017. Ref.3 | ||||||
| Sequence conflict | 731 | 1 | A → G Ref.1 | ||||||
| Sequence conflict | 731 | 1 | A → G Ref.4 | ||||||
| Sequence conflict | 744 | 1 | D → E Ref.1 | ||||||
| Sequence conflict | 744 | 1 | D → E Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Novel nuclear matrix protein HET binds to and influences activity of the HSP27 promoter in human breast cancer cells." Oesterreich S., Lee A.V., Sullivan T.M., Samuel S.K., Davie J.R., Fuqua S.A.W. J. Cell. Biochem. 67:275-286(1997) [PubMed: 9328833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Mammary carcinoma. |
| [2] | "The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M.Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-753. Tissue: Brain. |
| [4] | "Purification and molecular cloning of the scaffold attachment factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR-DNA." Renz A., Fackelmayer F.O. Nucleic Acids Res. 24:843-849(1996) [PubMed: 8600450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 52-915, CHARACTERIZATION, PHOSPHORYLATION. Tissue: Cervix carcinoma. |
| [5] | "SAF-B couples transcription and pre-mRNA splicing to SAR/MAR elements." Nayler O., Straetling W., Bourquin J.-P., Stagljar I., Lindemann L., Jasper H., Hartmann A.M., Fackelmeyer F.O., Ullrich A., Stamm S. Nucleic Acids Res. 26:3542-3549(1998) [PubMed: 9671816] [Abstract] Cited for: INTERACTION WITH POLR2A. |
| [6] | "SAFB2, a new scaffold attachment factor homolog and estrogen receptor corepressor." Townson S.M., Dobrzycka K.M., Lee A.V., Air M., Deng W., Kang K., Jiang S., Kioka N., Michaelis K., Oesterreich S. J. Biol. Chem. 278:20059-20068(2003) [PubMed: 12660241] [Abstract] Cited for: CHARACTERIZATION. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384; SER-589; SER-604 AND SER-808, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288; SER-601 AND SER-604, MASS SPECTROMETRY. Tissue: Epithelium. |
| [9] | "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-604, MASS SPECTROMETRY. Tissue: Epithelium. |
| [10] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-555 AND SER-557, MASS SPECTROMETRY. |
| [11] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-373; SER-383; SER-384; SER-601 AND SER-604, MASS SPECTROMETRY. |
| [12] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U72355 mRNA. Translation: AAC00056.1. AC004611 Genomic DNA. Translation: AAC14667.1. AC011499 Genomic DNA. No translation available. AC134303 Genomic DNA. No translation available. AB208780 mRNA. Translation: BAD92017.1. Different initiation. L43631 mRNA. Translation: AAC18697.1. | |
| IPI | IPI00300631. |
| PIR | S64732. |
| RefSeq | NP_002958.2. |
| UniGene | Hs.23978 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HD1 based on UniProtKB Q14103. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q15424. 2 interactions. |
PTM databases | |
| PhosphoSite | Q15424. |
Proteomic databases | |
| PRIDE | Q15424. |
Genome annotation databases | |
| Ensembl | ENSG00000160633. Homo sapiens. [Contig view] |
| GeneID | 6294. |
| KEGG | hsa:6294. |
Organism-specific databases | |
| GeneCards | GC19P005574. |
| HGNC | HGNC:10520. SAFB. |
| HPA | CAB001969. HPA016832. |
| MIM | 602895. gene. |
| PharmGKB | PA34928. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | Q15424. |
| HOVERGEN | Q15424. |
| OMA | Q15424. EDDRAWQ. |
Gene expression databases | |
| ArrayExpress | Q15424. |
| Bgee | Q15424. |
| CleanEx | HS_SAFB. |
| GermOnline | ENSG00000160633. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR012677. a_b_plait_nuc_bd. IPR000504. RRM_RNP1. IPR003034. SAP_DNA_bd. [Graphical view] |
| Gene3D | G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit. |
| Pfam | PF00076. RRM_1. 1 hit. PF02037. SAP. 1 hit. [Graphical view] |
| SMART | SM00360. RRM. 1 hit. SM00513. SAP. 1 hit. [Graphical view] |
| PROSITE | PS50102. RRM. 1 hit. PS50800. SAP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 24435. |
| SOURCE | Search... |
Entry information
| Entry name | SAFB1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15424 Secondary accession number(s): O60406, Q59HH8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


