Q15415 (RBY1F_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding motif protein, Y chromosome, family 1 member F/J

Alternative name(s):
Y chromosome RNA recognition motif 2

Gene names

Name:	RBMY1F
Synonyms:	YRRM2
AND
Name:	RBMY1J

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA-binding protein which may be involved in spermatogenesis. Required for sperm development, possibly by participating in pre-mRNA splicing in the testis. Ref.1
Subunit structure	Interacts with splicing factor proteins SFRS3/SRP20, TRA2B/SFRS10, KHDRBS1/SAM68 and KHDRBS3.
Subcellular location	Nucleus.
Tissue specificity	Testis-specific. Ref.1
Developmental stage	Expressed in all of the transcriptionally active stages of germ cell development from spermatogonia through spermatocytes to round spermatids.
Miscellaneous	The RBMY1 proteins are encoded by repeated regions of the Y chromosome, mostly within the AZFb region. The exact number of functional copies is unclear and may vary between individuals, and some of them may represent pseudogenes. The proteins are very similar, which makes the characterization of each protein difficult. Thus, most experiments do not discriminate between the different members. One can therefore suppose that reported interactions with a RBMY1 protein involve all the proteins.
Sequence similarities	Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Non-traceable author statement Ref.1. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15415-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15415-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-419: YGTSHGAPPARGPRMSYGGSTCH → DLSWCTTCKRASDVLWWKHLPRI
     420-496: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	RNA-binding motif protein, Y chromosome, family 1 member F/J		PRO_0000314164
Regions
	Domain	8 – 85	78	RRM
	Compositional bias	254 – 340	87	Arg-rich
Natural variations
	Alternative sequence	397 – 419	23	YGTSH…GSTCH → DLSWCTTCKRASDVLWWKHL PRI in isoform 2.		VSP_030217
	Alternative sequence	420 – 496	77	Missing in isoform 2.		VSP_030218
Experimental info
	Sequence conflict	7	1	P → A in CAA53660. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 66AC50828D44E9A8	FASTA	496	55,728
	10 20 30 40 50 60 MVEADHPGKL FIGGLNRETN EKMLKAVFGK HGPISEVLLI KDRTSKSRGF AFITFENPAD 70 80 90 100 110 120 AKNAAKDMNG TSLHGKAIKV EQAKKPSFQS GGRRRPPASS RNRSPSGSLR SARGSSGGTR 130 140 150 160 170 180 GWLPSHEGHL DDGGYTPDLK MSYSRGLIPV KRGPSSRSGG PPPKKSAPSA VARSNSWMGS 190 200 210 220 230 240 QGPMSQRREN YGVPPRRATI SSWRNDRMST RHDGYATNDG NHPSCQETRD YAPPSRGYAY 250 260 270 280 290 300 RDNGHSNRDE HSSRGYRNHR SSRETRDYAP PSRGHAYRDY GHSRRDESYS RGYRNHRSSR 310 320 330 340 350 360 ETREYAPPSR GHGYRDYGHS RRHESYSRGY RNHPSSRETR DYAPPHRDYA YRDYGHSSWD 370 380 390 400 410 420 EHSSRGYSYH DGYGEALGRD HSEHLSGSSY RDALQRYGTS HGAPPARGPR MSYGGSTCHA 430 440 450 460 470 480 YSNTRDRYGR SWESYSSCGD FHYCDREHVC RKDQRNPPSL GRVLPDPREA YGSSSYVASI 490 VDGGESRSEK GDSSRY
	Isoform 2 [UniParc]. Checksum: 82D82D712CD1934E	FASTA	419	47,454
	10 20 30 40 50 60 MVEADHPGKL FIGGLNRETN EKMLKAVFGK HGPISEVLLI KDRTSKSRGF AFITFENPAD 70 80 90 100 110 120 AKNAAKDMNG TSLHGKAIKV EQAKKPSFQS GGRRRPPASS RNRSPSGSLR SARGSSGGTR 130 140 150 160 170 180 GWLPSHEGHL DDGGYTPDLK MSYSRGLIPV KRGPSSRSGG PPPKKSAPSA VARSNSWMGS 190 200 210 220 230 240 QGPMSQRREN YGVPPRRATI SSWRNDRMST RHDGYATNDG NHPSCQETRD YAPPSRGYAY 250 260 270 280 290 300 RDNGHSNRDE HSSRGYRNHR SSRETRDYAP PSRGHAYRDY GHSRRDESYS RGYRNHRSSR 310 320 330 340 350 360 ETREYAPPSR GHGYRDYGHS RRHESYSRGY RNHPSSRETR DYAPPHRDYA YRDYGHSSWD 370 380 390 400 410 EHSSRGYSYH DGYGEALGRD HSEHLSGSSY RDALQRDLSW CTTCKRASDV LWWKHLPRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Y chromosome gene family with RNA-binding protein homology: candidates for the azoospermia factor AZF controlling human spermatogenesis." Ma K., Inglis J.D., Sharkey A., Bickmore W.A., Hill R.E., Prosser E.J., Speedson R.M., Thomson E.J., Jobling M. Cell 75:1287-1295(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[3]	"The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes." Skaletsky H., Kuroda-Kawaguchi T., Minx P.J., Cordum H.S., Hillier L.W., Brown L.G., Repping S., Pyntikova T., Ali J., Bieri T., Chinwalla A., Delehaunty A., Delehaunty K., Du H., Fewell G., Fulton L., Fulton R., Graves T.A. , Hou S.-F., Latrielle P., Leonard S., Mardis E., Maupin R., McPherson J., Miner T., Nash W., Nguyen C., Ozersky P., Pepin K., Rock S., Rohlfing T., Scott K., Schultz B., Strong C., Tin-Wollam A., Yang S.-P., Waterston R.H., Wilson R.K., Rozen S., Page D.C. Nature 423:825-837(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76060 mRNA. Translation: CAA53660.1. AK313597 mRNA. Translation: BAG36363.1. AC023342 Genomic DNA. No translation available. AC007320 Genomic DNA. No translation available. BC030018 mRNA. Translation: AAH30018.1. BC152392 mRNA. Translation: AAI52393.1.
IPI	IPI00413949. IPI00879122.
PIR	B49418.
RefSeq	NP_001006117.2. NM_001006117.2. NP_689798.1. NM_152585.1.
UniGene	Hs.567746.
3D structure databases
HSSP	HSSP built from PDB template 1X5S based on UniProtKB Q14011.
ProteinModelPortal	Q15415.
SMR	Q15415. Positions 1-108.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000250831.
PTM databases
PhosphoSite	Q15415.
Proteomic databases
PaxDb	Q15415.
PRIDE	Q15415.
Protocols and materials databases
DNASU	159163. 378951.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250831; ENSP00000250831; ENSG00000226941. ENST00000303766; ENSP00000307155; ENSG00000169800. ENST00000414629; ENSP00000405745; ENSG00000226941.
GeneID	159163. 378951.
KEGG	hsa:159163. hsa:378951.
UCSC	uc004fva.3. human. uc010nxh.3. human.
Organism-specific databases
CTD	159163. 378951.
GeneCards	GC0YM024314. GC0YP024456.
HGNC	HGNC:23974. RBMY1F. HGNC:23917. RBMY1J.
HPA	HPA001534.
MIM	400006. gene.
neXtProt	NX_Q15415.
PharmGKB	PA134946298.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG140744.
HOGENOM	HOG000276235.
HOVERGEN	HBG063314.
InParanoid	Q15415.
OMA	KDMNGTS.
OrthoDB	EOG4TF0N7.
PhylomeDB	Q15415.
Gene expression databases
Bgee	Q15415.
CleanEx	HS_RBMY1F.
Genevestigator	Q15415.
Family and domain databases
Gene3D	3.30.70.330. 1 hit.
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR012604. RBM1CTR. IPR000504. RRM_dom. [Graphical view]
Pfam	PF08081. RBM1CTR. 1 hit. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
PROSITE	PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	87890.
SOURCE	Search...

Entry information

Entry name

RBY1F_HUMAN

Accession

Primary (citable) accession number: Q15415
Secondary accession number(s): B2R916

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.