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Protein

Disks large-associated protein 5

Gene

DLGAP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.3 Publications

GO - Molecular functioni

  • phosphoprotein phosphatase activity Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • mitotic chromosome movement towards spindle pole Source: UniProtKB
  • positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB
  • signaling Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciZFISH:ENSG00000126787-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Disks large-associated protein 5
Short name:
DAP-5
Alternative name(s):
Discs large homolog 7
Disks large-associated protein DLG7
Hepatoma up-regulated protein
Short name:
HURP
Gene namesi
Name:DLGAP5
Synonyms:DLG7, KIAA0008
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:16864. DLGAP5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • microtubule organizing center Source: HPA
  • nucleus Source: UniProtKB
  • spindle pole centrosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9787.
OpenTargetsiENSG00000126787.
PharmGKBiPA162383761.

Polymorphism and mutation databases

BioMutaiDLGAP5.
DMDMi82592583.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001742991 – 846Disks large-associated protein 5Add BLAST846

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei202PhosphoserineCombined sources1
Modified residuei326PhosphothreonineCombined sources1
Modified residuei329Phosphothreonine; by CDK1Combined sources1 Publication1
Modified residuei338PhosphothreonineCombined sources1
Modified residuei401Phosphothreonine; by CDK11 Publication1
Modified residuei402Phosphothreonine; by CDK11 Publication1
Modified residuei618Phosphoserine; by CDK1Combined sources1 Publication1
Modified residuei627Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei629PhosphoserineCombined sources1
Modified residuei634PhosphoserineCombined sources1
Modified residuei639Phosphothreonine; by CDK11 Publication1
Modified residuei642Phosphoserine; by CDK11 Publication1
Modified residuei662PhosphoserineCombined sources1
Modified residuei725Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei757Phosphoserine; by AURKA1 Publication1
Modified residuei759Phosphothreonine; by CDK11 Publication1
Modified residuei774PhosphoserineCombined sources1
Modified residuei777PhosphoserineCombined sources1
Modified residuei784PhosphothreonineCombined sources1
Modified residuei806PhosphoserineCombined sources1
Modified residuei812PhosphoserineCombined sources1
Modified residuei830Phosphoserine; by AURKACombined sources1 Publication1
Modified residuei839Phosphoserine; by CDK1Combined sources1 Publication1

Post-translational modificationi

Ubiquitinated, leading to its degradation.1 Publication
Decreased phosphorylation levels are associated with the differentiation of intestinal epithelial cells.3 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15398.
PaxDbiQ15398.
PeptideAtlasiQ15398.
PRIDEiQ15398.

PTM databases

iPTMnetiQ15398.
PhosphoSitePlusiQ15398.

Expressioni

Tissue specificityi

Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.1 Publication

Developmental stagei

Elevated levels of expression detected in the G2/M phase of synchronized cultures of HeLa cells.1 Publication

Gene expression databases

BgeeiENSG00000126787.
CleanExiHS_DLGAP5.
ExpressionAtlasiQ15398. baseline and differential.
GenevisibleiQ15398. HS.

Organism-specific databases

HPAiHPA005546.

Interactioni

Subunit structurei

Interacts with CDK1. Interacts with the C-terminal proline-rich region of FBXO7. Recruited by FBXO7 to a SCF (SKP1-CUL1-F-box) protein complex in a CDK1/Cyclin B-phosphorylation dependent manner. Interacts with CDH1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GOLGA2Q083793EBI-748280,EBI-618309
MTUS2Q5JR593EBI-748280,EBI-742948

Protein-protein interaction databases

BioGridi115131. 20 interactors.
IntActiQ15398. 8 interactors.
MINTiMINT-1465344.
STRINGi9606.ENSP00000247191.

Structurei

3D structure databases

ProteinModelPortaliQ15398.
SMRiQ15398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili90 – 120Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the SAPAP family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOGENOMiHOG000054220.
HOVERGENiHBG062172.
InParanoidiQ15398.
KOiK16804.
OMAiYRPDMPC.
OrthoDBiEOG091G0224.
PhylomeDBiQ15398.
TreeFamiTF321382.

Family and domain databases

InterProiIPR005026. SAPAP.
[Graphical view]
PfamiPF03359. GKAP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15398-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSHFASRH RKDISTEMIR TKIAHRKSLS QKENRHKEYE RNRHFGLKDV
60 70 80 90 100
NIPTLEGRIL VELDETSQGL VPEKTNVKPR AMKTILGDQR KQMLQKYKEE
110 120 130 140 150
KQLQKLKEQR EKAKRGIFKV GRYRPDMPCF LLSNQNAVKA EPKKAIPSSV
160 170 180 190 200
RITRSKAKDQ MEQTKIDNES DVRAIRPGPR QTSEKKVSDK EKKVVQPVMP
210 220 230 240 250
TSLRMTRSAT QAAKQVPRTV SSTTARKPVT RAANENEPEG KVPSKGRPAK
260 270 280 290 300
NVETKPDKGI SCKVDSEENT LNSQTNATSG MNPDGVLSKM ENLPEINTAK
310 320 330 340 350
IKGKNSFAPK DFMFQPLDGL KTYQVTPMTP RSANAFLTPS YTWTPLKTEV
360 370 380 390 400
DESQATKEIL AQKCKTYSTK TIQQDSNKLP CPLGPLTVWH EEHVLNKNEA
410 420 430 440 450
TTKNLNGLPI KEVPSLERNE GRIAQPHHGV PYFRNILQSE TEKLTSHCFE
460 470 480 490 500
WDRKLELDIP DDAKDLIRTA VGQTRLLMKE RFKQFEGLVD DCEYKRGIKE
510 520 530 540 550
TTCTDLDGFW DMVSFQIEDV IHKFNNLIKL EESGWQVNNN MNHNMNKNVF
560 570 580 590 600
RKKVVSGIAS KPKQDDAGRI AARNRLAAIK NAMRERIRQE ECAETAVSVI
610 620 630 640 650
PKEVDKIVFD AGFFRVESPV KLFSGLSVSS EGPSQRLGTP KSVNKAVSQS
660 670 680 690 700
RNEMGIPQQT TSPENAGPQN TKSEHVKKTL FLSIPESRSS IEDAQCPGLP
710 720 730 740 750
DLIEENHVVN KTDLKVDCLS SERMSLPLLA GGVADDINTN KKEGISDVVE
760 770 780 790 800
GMELNSSITS QDVLMSSPEK NTASQNSILE EGETKISQSE LFDNKSLTTE
810 820 830 840
CHLLDSPGLN CSNPFTQLER RHQEHARHIS FGGNLITFSP LQPGEF
Length:846
Mass (Da):95,115
Last modified:September 13, 2005 - v2
Checksum:i588BAF238D6FFB72
GO
Isoform 2 (identifier: Q15398-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: Missing.

Note: No experimental confirmation available.
Show »
Length:765
Mass (Da):85,668
Checksum:i00AFF91A02387EA1
GO
Isoform 3 (identifier: Q15398-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     807-846: PGLNCSNPFT...TFSPLQPGEF → VGSCYVARAG...AGTTARSKLQ

Note: No experimental confirmation available.
Show »
Length:842
Mass (Da):94,178
Checksum:iFDEF1E4B900F758C
GO

Sequence cautioni

The sequence BAA02797 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAD62583 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253E → K in BAA02797 (PubMed:7584026).Curated1
Sequence conflicti328M → T in BAG61340 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02377469G → E.1 PublicationCorresponds to variant rs2274271dbSNPEnsembl.1
Natural variantiVAR_057718324Q → H.Corresponds to variant rs8010791dbSNPEnsembl.1
Natural variantiVAR_057719469T → I.Corresponds to variant rs17128275dbSNPEnsembl.1
Natural variantiVAR_062147753E → Q.Corresponds to variant rs35954941dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0155501 – 81Missing in isoform 2. 1 PublicationAdd BLAST81
Alternative sequenceiVSP_045341807 – 846PGLNC…QPGEF → VGSCYVARAGLEVLGSSDPT TSASRVAGTTARSKLQ in isoform 3. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076695 mRNA. Translation: BAB97376.1.
D13633 mRNA. Translation: BAA02797.3. Different initiation.
BX248255 mRNA. Translation: CAD62583.1. Different initiation.
BT007344 mRNA. Translation: AAP36008.1.
AK299338 mRNA. Translation: BAG61340.1.
AL139316 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80664.1.
BC010658 mRNA. Translation: AAH10658.2.
BC016276 mRNA. Translation: AAH16276.2.
CCDSiCCDS53897.1. [Q15398-3]
CCDS9723.1. [Q15398-2]
RefSeqiNP_001139487.1. NM_001146015.1. [Q15398-3]
NP_055565.3. NM_014750.4. [Q15398-2]
XP_016877329.1. XM_017021840.1. [Q15398-2]
UniGeneiHs.77695.

Genome annotation databases

EnsembliENST00000247191; ENSP00000247191; ENSG00000126787. [Q15398-2]
ENST00000395425; ENSP00000378815; ENSG00000126787. [Q15398-3]
GeneIDi9787.
KEGGihsa:9787.
UCSCiuc001xbs.4. human. [Q15398-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB076695 mRNA. Translation: BAB97376.1.
D13633 mRNA. Translation: BAA02797.3. Different initiation.
BX248255 mRNA. Translation: CAD62583.1. Different initiation.
BT007344 mRNA. Translation: AAP36008.1.
AK299338 mRNA. Translation: BAG61340.1.
AL139316 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80664.1.
BC010658 mRNA. Translation: AAH10658.2.
BC016276 mRNA. Translation: AAH16276.2.
CCDSiCCDS53897.1. [Q15398-3]
CCDS9723.1. [Q15398-2]
RefSeqiNP_001139487.1. NM_001146015.1. [Q15398-3]
NP_055565.3. NM_014750.4. [Q15398-2]
XP_016877329.1. XM_017021840.1. [Q15398-2]
UniGeneiHs.77695.

3D structure databases

ProteinModelPortaliQ15398.
SMRiQ15398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115131. 20 interactors.
IntActiQ15398. 8 interactors.
MINTiMINT-1465344.
STRINGi9606.ENSP00000247191.

PTM databases

iPTMnetiQ15398.
PhosphoSitePlusiQ15398.

Polymorphism and mutation databases

BioMutaiDLGAP5.
DMDMi82592583.

Proteomic databases

EPDiQ15398.
PaxDbiQ15398.
PeptideAtlasiQ15398.
PRIDEiQ15398.

Protocols and materials databases

DNASUi9787.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000247191; ENSP00000247191; ENSG00000126787. [Q15398-2]
ENST00000395425; ENSP00000378815; ENSG00000126787. [Q15398-3]
GeneIDi9787.
KEGGihsa:9787.
UCSCiuc001xbs.4. human. [Q15398-2]

Organism-specific databases

CTDi9787.
DisGeNETi9787.
GeneCardsiDLGAP5.
H-InvDBHIX0011684.
HGNCiHGNC:16864. DLGAP5.
HPAiHPA005546.
neXtProtiNX_Q15398.
OpenTargetsiENSG00000126787.
PharmGKBiPA162383761.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3971. Eukaryota.
ENOG4111JKV. LUCA.
GeneTreeiENSGT00550000074473.
HOGENOMiHOG000054220.
HOVERGENiHBG062172.
InParanoidiQ15398.
KOiK16804.
OMAiYRPDMPC.
OrthoDBiEOG091G0224.
PhylomeDBiQ15398.
TreeFamiTF321382.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000126787-MONOMER.

Miscellaneous databases

GeneWikiiDLGAP5.
GenomeRNAii9787.
PROiQ15398.

Gene expression databases

BgeeiENSG00000126787.
CleanExiHS_DLGAP5.
ExpressionAtlasiQ15398. baseline and differential.
GenevisibleiQ15398. HS.

Family and domain databases

InterProiIPR005026. SAPAP.
[Graphical view]
PfamiPF03359. GKAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLGP5_HUMAN
AccessioniPrimary (citable) accession number: Q15398
Secondary accession number(s): A8MTM6
, B4DRM8, Q86T11, Q8NG58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It was localized to the spindle and the spindle pole (PubMed:12527899) but was later found to be localized to the spindle and to be excluded from the spindle pole (PubMed:15561729).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.