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Protein

Pumilio homolog 3

Gene

PUM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress (PubMed:21266351). Binds to double-stranded RNA or DNA without sequence specificity (PubMed:25512524). Involved in development of the eye and of primordial germ cells (By similarity).By similarity2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • mRNA binding Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • regulation of protein ADP-ribosylation Source: UniProtKB
  • regulation of translation Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000080608-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 3Curated
Alternative name(s):
HBV X-transactivated gene 5 protein
HBV XAg-transactivated protein 5
Minor histocompatibility antigen HA-8
Short name:
HLA-HA8
Gene namesi
Name:PUM3Imported
Synonyms:cPERP-C1 Publication, KIAA0020Imported, PUF-A1 Publication, XTP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:29676. PUM3.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9933.
OpenTargetsiENSG00000080608.
PharmGKBiPA134895115.

Polymorphism and mutation databases

BioMutaiKIAA0020.
DMDMi81175177.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759291 – 648Pumilio homolog 3Add BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ15397.
MaxQBiQ15397.
PaxDbiQ15397.
PeptideAtlasiQ15397.
PRIDEiQ15397.

2D gel databases

SWISS-2DPAGEQ15397.

PTM databases

iPTMnetiQ15397.
PhosphoSitePlusiQ15397.
SwissPalmiQ15397.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000080608.
CleanExiHS_KIAA0020.
ExpressionAtlasiQ15397. baseline and differential.
GenevisibleiQ15397. HS.

Organism-specific databases

HPAiHPA002353.

Interactioni

Subunit structurei

Interacts with PARP1 (via catalytic domain).1 Publication

Protein-protein interaction databases

BioGridi115259. 126 interactors.
IntActiQ15397. 12 interactors.
MINTiMINT-1404790.
STRINGi9606.ENSP00000380982.

Structurei

Secondary structure

1648
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 145Combined sources14
Beta strandi147 – 149Combined sources3
Helixi152 – 166Combined sources15
Turni167 – 169Combined sources3
Helixi170 – 175Combined sources6
Turni177 – 179Combined sources3
Helixi180 – 189Combined sources10
Helixi192 – 202Combined sources11
Helixi203 – 205Combined sources3
Helixi206 – 210Combined sources5
Helixi213 – 225Combined sources13
Helixi228 – 238Combined sources11
Turni239 – 241Combined sources3
Helixi242 – 245Combined sources4
Helixi249 – 261Combined sources13
Helixi265 – 272Combined sources8
Helixi273 – 276Combined sources4
Helixi278 – 283Combined sources6
Beta strandi286 – 288Combined sources3
Helixi291 – 297Combined sources7
Helixi299 – 301Combined sources3
Helixi302 – 313Combined sources12
Helixi314 – 318Combined sources5
Helixi320 – 323Combined sources4
Helixi326 – 338Combined sources13
Helixi341 – 351Combined sources11
Turni352 – 354Combined sources3
Helixi355 – 358Combined sources4
Helixi362 – 374Combined sources13
Helixi377 – 386Combined sources10
Turni387 – 390Combined sources4
Helixi391 – 395Combined sources5
Turni398 – 400Combined sources3
Helixi401 – 410Combined sources10
Helixi414 – 427Combined sources14
Helixi429 – 433Combined sources5
Helixi436 – 446Combined sources11
Turni451 – 453Combined sources3
Helixi456 – 463Combined sources8
Turni464 – 467Combined sources4
Helixi475 – 496Combined sources22
Helixi498 – 502Combined sources5
Helixi507 – 509Combined sources3
Helixi510 – 517Combined sources8
Helixi523 – 531Combined sources9
Helixi548 – 550Combined sources3
Helixi554 – 570Combined sources17
Helixi577 – 585Combined sources9
Helixi587 – 592Combined sources6
Helixi593 – 595Combined sources3
Helixi597 – 607Combined sources11
Helixi612 – 622Combined sources11
Helixi623 – 625Combined sources3
Helixi626 – 629Combined sources4
Helixi637 – 645Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WZRX-ray2.15A/B123-648[»]
4WZWX-ray2.95A129-648[»]
ProteinModelPortaliQ15397.
SMRiQ15397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 510PUM-HDPROSITE-ProRule annotationAdd BLAST368
Repeati177 – 212Pumilio 11 PublicationAdd BLAST36
Repeati213 – 248Pumilio 21 PublicationAdd BLAST36
Repeati249 – 277Pumilio 31 PublicationAdd BLAST29
Repeati289 – 325Pumilio 41 PublicationAdd BLAST37
Repeati326 – 361Pumilio 51 PublicationAdd BLAST36
Repeati362 – 397Pumilio 61 PublicationAdd BLAST36
Repeati398 – 435Pumilio 71 PublicationAdd BLAST38
Repeati436 – 504Pumilio 81 PublicationAdd BLAST69
Repeati505 – 551Pumilio 91 PublicationAdd BLAST47
Repeati552 – 596Pumilio 101 PublicationAdd BLAST45
Repeati597 – 636Pumilio 111 PublicationAdd BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 297HA-89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 118Nuclear localization signal1 PublicationAdd BLAST13

Domaini

The HA-8 region can be cleaved and exposed at the cell surface where it plays a role as a minor histocompatibility HLA-A*0201-restricted antigen.
A 90 degree bend between Pumilio repeats 3 and 4 gives rise to a L-shaped protein.1 Publication

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 11 pumilio repeats.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
GeneTreeiENSGT00390000015757.
HOVERGENiHBG052166.
InParanoidiQ15397.
KOiK14844.
OMAiYLQEHAQ.
OrthoDBiEOG091G06QP.
PhylomeDBiQ15397.
TreeFamiTF312954.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 5 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKGKKQFT GKSTKTAQEK NRFHKNSDSG SSKTFPTRKV AKEGGPKVTS
60 70 80 90 100
RNFEKSITKL GKKGVKQFKN KQQGDKSPKN KFQPANKFNK KRKFQPDGRS
110 120 130 140 150
DESAAKKPKW DDFKKKKKEL KQSRQLSDKT NYDIVVRAKQ MWEILRRKDC
160 170 180 190 200
DKEKRVKLMS DLQKLIQGKI KTIAFAHDST RVIQCYIQYG NEEQRKQAFE
210 220 230 240 250
ELRDDLVELS KAKYSRNIVK KFLMYGSKPQ IAEIIRSFKG HVRKMLRHAE
260 270 280 290 300
ASAIVEYAYN DKAILEQRNM LTEELYGNTF QLYKSADHRT LDKVLEVQPE
310 320 330 340 350
KLELIMDEMK QILTPMAQKE AVIKHSLVHK VFLDFFTYAP PKLRSEMIEA
360 370 380 390 400
IREAVVYLAH THDGARVAMH CLWHGTPKDR KVIVKTMKTY VEKVANGQYS
410 420 430 440 450
HLVLLAAFDC IDDTKLVKQI IISEIISSLP SIVNDKYGRK VLLYLLSPRD
460 470 480 490 500
PAHTVREIIE VLQKGDGNAH SKKDTEVRRR ELLESISPAL LSYLQEHAQE
510 520 530 540 550
VVLDKSACVL VSDILGSATG DVQPTMNAIA SLAATGLHPG GKDGELHIAE
560 570 580 590 600
HPAGHLVLKW LIEQDKKMKE NGREGCFAKT LVEHVGMKNL KSWASVNRGA
610 620 630 640
IILSSLLQSC DLEVANKVKA ALKSLIPTLE KTKSTSKGIE ILLEKLST
Length:648
Mass (Da):73,584
Last modified:November 8, 2005 - v3
Checksum:i449BFBF22F1BBA86
GO

Sequence cautioni

The sequence BAA02808 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI12924 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI15123 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti625L → S in CAG33238 (Ref. 8) Curated1

Polymorphismi

The following alleles of HA-8 are known: HA-8R, HA-8P, HA-8PL, of which only HA-8R leads to specific cytotoxic T lymphocyte (CTL) recognition. The lack of CTL recognition of cells expressing HA-8P may be due to impaired transport associated with antigen processing. The sequence shown is that of HA-8R.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05161313S → N.1 PublicationCorresponds to variant rs10968457dbSNPEnsembl.1
Natural variantiVAR_051614264I → V.Corresponds to variant rs35869387dbSNPEnsembl.1
Natural variantiVAR_023772289R → P in allele HA-8P and allele HA-8PL. 2 PublicationsCorresponds to variant rs2173904dbSNPEnsembl.1
Natural variantiVAR_023773297V → L in allele HA-8PL. 2 PublicationsCorresponds to variant rs2270891dbSNPEnsembl.1
Natural variantiVAR_051615414T → S.Corresponds to variant rs3736390dbSNPEnsembl.1
Natural variantiVAR_051616480R → Q.Corresponds to variant rs2270889dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292169 mRNA. Translation: BAF84858.1.
AL354723, AL589675 Genomic DNA. Translation: CAI15123.2. Sequence problems.
AL589675, AL354723 Genomic DNA. Translation: CAI12924.2. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58802.1.
BC016137 mRNA. Translation: AAH16137.2.
D13645 mRNA. Translation: BAA02808.1. Different initiation.
AF490254 mRNA. Translation: AAO85462.1.
CR456957 mRNA. Translation: CAG33238.1.
AY047588 mRNA. Translation: AAL06072.1.
AY047589 mRNA. Translation: AAL06073.1.
AY047590 mRNA. Translation: AAL06074.1.
CCDSiCCDS6448.2.
RefSeqiNP_055693.4. NM_014878.4.
UniGeneiHs.493309.

Genome annotation databases

EnsembliENST00000397885; ENSP00000380982; ENSG00000080608.
GeneIDi9933.
KEGGihsa:9933.
UCSCiuc003zhp.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292169 mRNA. Translation: BAF84858.1.
AL354723, AL589675 Genomic DNA. Translation: CAI15123.2. Sequence problems.
AL589675, AL354723 Genomic DNA. Translation: CAI12924.2. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58802.1.
BC016137 mRNA. Translation: AAH16137.2.
D13645 mRNA. Translation: BAA02808.1. Different initiation.
AF490254 mRNA. Translation: AAO85462.1.
CR456957 mRNA. Translation: CAG33238.1.
AY047588 mRNA. Translation: AAL06072.1.
AY047589 mRNA. Translation: AAL06073.1.
AY047590 mRNA. Translation: AAL06074.1.
CCDSiCCDS6448.2.
RefSeqiNP_055693.4. NM_014878.4.
UniGeneiHs.493309.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WZRX-ray2.15A/B123-648[»]
4WZWX-ray2.95A129-648[»]
ProteinModelPortaliQ15397.
SMRiQ15397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115259. 126 interactors.
IntActiQ15397. 12 interactors.
MINTiMINT-1404790.
STRINGi9606.ENSP00000380982.

PTM databases

iPTMnetiQ15397.
PhosphoSitePlusiQ15397.
SwissPalmiQ15397.

Polymorphism and mutation databases

BioMutaiKIAA0020.
DMDMi81175177.

2D gel databases

SWISS-2DPAGEQ15397.

Proteomic databases

EPDiQ15397.
MaxQBiQ15397.
PaxDbiQ15397.
PeptideAtlasiQ15397.
PRIDEiQ15397.

Protocols and materials databases

DNASUi9933.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397885; ENSP00000380982; ENSG00000080608.
GeneIDi9933.
KEGGihsa:9933.
UCSCiuc003zhp.2. human.

Organism-specific databases

CTDi9933.
DisGeNETi9933.
GeneCardsiKIAA0020.
HGNCiHGNC:29676. PUM3.
HPAiHPA002353.
MIMi609960. gene.
neXtProtiNX_Q15397.
OpenTargetsiENSG00000080608.
PharmGKBiPA134895115.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
GeneTreeiENSGT00390000015757.
HOVERGENiHBG052166.
InParanoidiQ15397.
KOiK14844.
OMAiYLQEHAQ.
OrthoDBiEOG091G06QP.
PhylomeDBiQ15397.
TreeFamiTF312954.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000080608-MONOMER.

Miscellaneous databases

ChiTaRSiKIAA0020. human.
GeneWikiiKIAA0020.
GenomeRNAii9933.
PROiQ15397.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000080608.
CleanExiHS_KIAA0020.
ExpressionAtlasiQ15397. baseline and differential.
GenevisibleiQ15397. HS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 5 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUM3_HUMAN
AccessioniPrimary (citable) accession number: Q15397
Secondary accession number(s): A8K804
, Q547G7, Q5SZY9, Q6IB47, Q96B27, Q96L78, Q96L79, Q96L80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.