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Q15397 (K0020_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pumilio domain-containing protein KIAA0020
Alternative name(s):
HBV X-transactivated gene 5 protein
HBV XAg-transactivated protein 5
Minor histocompatibility antigen HA-8
Short name=HLA-HA8
Gene names
Name:KIAA0020
Synonyms:XTP5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Nucleusnucleolus Ref.6 Ref.10.

Tissue specificity

Widely expressed. Ref.5

Domain

The HA-8 region can be cleaved and exposed at the cell surface where it plays a role as a minor histocompatibility HLA-A*0201-restricted antigen.

Polymorphism

The following alleles of HA-8 are known: HA-8R, HA-8P, HA-8PL, of which only HA-8R leads to specific cytotoxic T lymphocyte (CTL) recognition. The lack of CTL recognition of cells expressing HA-8P may be due to impaired transport associated with antigen processing. The sequence shown is that of HA-8R.

Sequence similarities

Contains 1 PUM-HD domain.

Contains 6 pumilio repeats.

Sequence caution

The sequence BAA02808.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAI12924.2 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI15123.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityPolymorphism
   DomainRepeat
   LigandRNA-binding
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum

Inferred from direct assay PubMed 10942595. Source: UniProtKB

nucleolus

Inferred from direct assay. Source: HPA

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22658674PubMed 22681889. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648Pumilio domain-containing protein KIAA0020
PRO_0000075929

Regions

Domain143 – 510368PUM-HD
Repeat165 – 20036Pumilio 1
Repeat201 – 23636Pumilio 2
Repeat237 – 27337Pumilio 3
Repeat350 – 38536Pumilio 4
Repeat386 – 42237Pumilio 5
Repeat424 – 46037Pumilio 6
Region289 – 2979HA-8

Amino acid modifications

Modified residue331N6-acetyllysine By similarity

Natural variations

Natural variant131S → N. Ref.4
Corresponds to variant rs10968457 [ dbSNP | Ensembl ].
VAR_051613
Natural variant2641I → V.
Corresponds to variant rs35869387 [ dbSNP | Ensembl ].
VAR_051614
Natural variant2891R → P in allele HA-8P and allele HA-8PL.
Corresponds to variant rs2173904 [ dbSNP | Ensembl ].
VAR_023772
Natural variant2971V → L in allele HA-8PL.
Corresponds to variant rs2270891 [ dbSNP | Ensembl ].
VAR_023773
Natural variant4141T → S.
Corresponds to variant rs3736390 [ dbSNP | Ensembl ].
VAR_051615
Natural variant4801R → Q.
Corresponds to variant rs2270889 [ dbSNP | Ensembl ].
VAR_051616

Experimental info

Sequence conflict6251L → S in CAG33238. Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q15397 [UniParc].

Last modified November 8, 2005. Version 3.
Checksum: 449BFBF22F1BBA86

FASTA64873,584
        10         20         30         40         50         60 
MEVKGKKQFT GKSTKTAQEK NRFHKNSDSG SSKTFPTRKV AKEGGPKVTS RNFEKSITKL 

        70         80         90        100        110        120 
GKKGVKQFKN KQQGDKSPKN KFQPANKFNK KRKFQPDGRS DESAAKKPKW DDFKKKKKEL 

       130        140        150        160        170        180 
KQSRQLSDKT NYDIVVRAKQ MWEILRRKDC DKEKRVKLMS DLQKLIQGKI KTIAFAHDST 

       190        200        210        220        230        240 
RVIQCYIQYG NEEQRKQAFE ELRDDLVELS KAKYSRNIVK KFLMYGSKPQ IAEIIRSFKG 

       250        260        270        280        290        300 
HVRKMLRHAE ASAIVEYAYN DKAILEQRNM LTEELYGNTF QLYKSADHRT LDKVLEVQPE 

       310        320        330        340        350        360 
KLELIMDEMK QILTPMAQKE AVIKHSLVHK VFLDFFTYAP PKLRSEMIEA IREAVVYLAH 

       370        380        390        400        410        420 
THDGARVAMH CLWHGTPKDR KVIVKTMKTY VEKVANGQYS HLVLLAAFDC IDDTKLVKQI 

       430        440        450        460        470        480 
IISEIISSLP SIVNDKYGRK VLLYLLSPRD PAHTVREIIE VLQKGDGNAH SKKDTEVRRR 

       490        500        510        520        530        540 
ELLESISPAL LSYLQEHAQE VVLDKSACVL VSDILGSATG DVQPTMNAIA SLAATGLHPG 

       550        560        570        580        590        600 
GKDGELHIAE HPAGHLVLKW LIEQDKKMKE NGREGCFAKT LVEHVGMKNL KSWASVNRGA 

       610        620        630        640 
IILSSLLQSC DLEVANKVKA ALKSLIPTLE KTKSTSKGIE ILLEKLST 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Synovium.
[2]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELE HA-8R).
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE HA-8PL), VARIANT ASN-13.
Tissue: Uterus.
[5]"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1."
Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S.
DNA Res. 1:27-35(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-648 (ALLELE HA-8R), TISSUE SPECIFICITY.
Tissue: Bone marrow.
[6]Bienvenut W.V.
Submitted (AUG-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 130-137; 294-301; 331-342; 345-352 AND 441-464, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Cervix carcinoma.
[7]"Cloning and identification of human gene 5 transactivated by hepatitis B virus X antigen."
Liu Y., Cheng J., Lu Y., Wang G., Li K., Chen J., Li L.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 141-648 (ALLELE HA-8R).
[8]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 141-648 (ALLELE HA-8R).
[9]"The immunogenicity of a new human minor histocompatibility antigen results from differential antigen processing."
Brickner A.G., Warren E.H., Caldwell J.A., Akatsuka Y., Golovina T.N., Zarling A.L., Shabanowitz J., Eisenlohr L.C., Hunt D.F., Engelhard V.H., Riddell S.R.
J. Exp. Med. 193:195-206(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-350 (ALLELES HA-8R; HA-8P AND HA-8PL), IDENTIFICATION BY MASS SPECTROMETRY OF HA-8.
Tissue: B-cell.
[10]"Functional proteomic analysis of human nucleolus."
Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J.
Mol. Biol. Cell 13:4100-4109(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK292169 mRNA. Translation: BAF84858.1.
AL354723, AL589675 Genomic DNA. Translation: CAI15123.2. Sequence problems.
AL589675, AL354723 Genomic DNA. Translation: CAI12924.2. Sequence problems.
CH471071 Genomic DNA. Translation: EAW58802.1.
BC016137 mRNA. Translation: AAH16137.2.
D13645 mRNA. Translation: BAA02808.1. Different initiation.
AF490254 mRNA. Translation: AAO85462.1.
CR456957 mRNA. Translation: CAG33238.1.
AY047588 mRNA. Translation: AAL06072.1.
AY047589 mRNA. Translation: AAL06073.1.
AY047590 mRNA. Translation: AAL06074.1.
CCDSCCDS6448.2.
RefSeqNP_055693.4. NM_014878.4.
UniGeneHs.493309.

3D structure databases

ProteinModelPortalQ15397.
SMRQ15397. Positions 166-464.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115259. 9 interactions.
IntActQ15397. 3 interactions.
MINTMINT-1404790.

PTM databases

PhosphoSiteQ15397.

Polymorphism databases

DMDM81175177.

2D gel databases

SWISS-2DPAGEQ15397.

Proteomic databases

MaxQBQ15397.
PaxDbQ15397.
PRIDEQ15397.

Protocols and materials databases

DNASU9933.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000397885; ENSP00000380982; ENSG00000080608.
GeneID9933.
KEGGhsa:9933.
UCSCuc003zhp.1. human.

Organism-specific databases

CTD9933.
GeneCardsGC09M002720.
HGNCHGNC:29676. KIAA0020.
HPAHPA002353.
MIM609960. gene.
neXtProtNX_Q15397.
PharmGKBPA134895115.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG273657.
HOVERGENHBG052166.
InParanoidQ15397.
KOK14844.
OMACLWHGTA.
OrthoDBEOG783MV2.
PhylomeDBQ15397.
TreeFamTF312954.

Gene expression databases

BgeeQ15397.
CleanExHS_KIAA0020.
GenevestigatorQ15397.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamPF08144. CPL. 1 hit.
[Graphical view]
SMARTSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
PROSITEPS50302. PUM. 5 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKIAA0020. human.
GeneWikiKIAA0020.
GenomeRNAi9933.
NextBio37470.
SOURCESearch...

Entry information

Entry nameK0020_HUMAN
AccessionPrimary (citable) accession number: Q15397
Secondary accession number(s): A8K804 expand/collapse secondary AC list , Q547G7, Q5SZY9, Q6IB47, Q96B27, Q96L78, Q96L79, Q96L80
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 8, 2005
Last modified: July 9, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM