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Protein

Pumilio homolog 3

Gene

PUM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress (PubMed:21266351). Binds to double-stranded RNA or DNA without sequence specificity (PubMed:25512524). Involved in development of the eye and of primordial germ cells (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • regulation of protein ADP-ribosylation Source: UniProtKB
  • regulation of translation Source: GO_Central

Keywordsi

Molecular functionDNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 3Curated
Alternative name(s):
HBV X-transactivated gene 5 protein
HBV XAg-transactivated protein 5
Minor histocompatibility antigen HA-8
Short name:
HLA-HA8
Gene namesi
Name:PUM3Imported
Synonyms:cPERP-C1 Publication, KIAA0020Imported, PUF-A1 Publication, XTP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000080608.9
HGNCiHGNC:29676 PUM3
MIMi609960 gene
neXtProtiNX_Q15397

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9933
OpenTargetsiENSG00000080608
PharmGKBiPA134895115

Polymorphism and mutation databases

BioMutaiKIAA0020
DMDMi81175177

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000759291 – 648Pumilio homolog 3Add BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ15397
MaxQBiQ15397
PaxDbiQ15397
PeptideAtlasiQ15397
PRIDEiQ15397
ProteomicsDBi60566

2D gel databases

SWISS-2DPAGEiQ15397

PTM databases

iPTMnetiQ15397
PhosphoSitePlusiQ15397
SwissPalmiQ15397

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSG00000080608
CleanExiHS_KIAA0020
ExpressionAtlasiQ15397 baseline and differential
GenevisibleiQ15397 HS

Organism-specific databases

HPAiHPA002353

Interactioni

Subunit structurei

Interacts with PARP1 (via catalytic domain).1 Publication

Protein-protein interaction databases

BioGridi115259, 134 interactors
IntActiQ15397, 14 interactors
MINTiQ15397
STRINGi9606.ENSP00000380982

Structurei

Secondary structure

1648
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 145Combined sources14
Beta strandi147 – 149Combined sources3
Helixi152 – 166Combined sources15
Turni167 – 169Combined sources3
Helixi170 – 175Combined sources6
Turni177 – 179Combined sources3
Helixi180 – 189Combined sources10
Helixi192 – 202Combined sources11
Helixi203 – 205Combined sources3
Helixi206 – 210Combined sources5
Helixi213 – 225Combined sources13
Helixi228 – 238Combined sources11
Turni239 – 241Combined sources3
Helixi242 – 245Combined sources4
Helixi249 – 261Combined sources13
Helixi265 – 272Combined sources8
Helixi273 – 276Combined sources4
Helixi278 – 283Combined sources6
Beta strandi286 – 288Combined sources3
Helixi291 – 297Combined sources7
Helixi299 – 301Combined sources3
Helixi302 – 313Combined sources12
Helixi314 – 318Combined sources5
Helixi320 – 323Combined sources4
Helixi326 – 338Combined sources13
Helixi341 – 351Combined sources11
Turni352 – 354Combined sources3
Helixi355 – 358Combined sources4
Helixi362 – 374Combined sources13
Helixi377 – 386Combined sources10
Turni387 – 390Combined sources4
Helixi391 – 395Combined sources5
Turni398 – 400Combined sources3
Helixi401 – 410Combined sources10
Helixi414 – 427Combined sources14
Helixi429 – 433Combined sources5
Helixi436 – 446Combined sources11
Turni451 – 453Combined sources3
Helixi456 – 463Combined sources8
Turni464 – 467Combined sources4
Helixi475 – 496Combined sources22
Helixi498 – 502Combined sources5
Helixi507 – 509Combined sources3
Helixi510 – 517Combined sources8
Helixi523 – 531Combined sources9
Helixi548 – 550Combined sources3
Helixi554 – 570Combined sources17
Helixi577 – 585Combined sources9
Helixi587 – 592Combined sources6
Helixi593 – 595Combined sources3
Helixi597 – 607Combined sources11
Helixi612 – 622Combined sources11
Helixi623 – 625Combined sources3
Helixi626 – 629Combined sources4
Helixi637 – 645Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WZRX-ray2.15A/B123-648[»]
4WZWX-ray2.95A129-648[»]
ProteinModelPortaliQ15397
SMRiQ15397
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 510PUM-HDPROSITE-ProRule annotationAdd BLAST368
Repeati177 – 212Pumilio 11 PublicationAdd BLAST36
Repeati213 – 248Pumilio 21 PublicationAdd BLAST36
Repeati249 – 277Pumilio 31 PublicationAdd BLAST29
Repeati289 – 325Pumilio 41 PublicationAdd BLAST37
Repeati326 – 361Pumilio 51 PublicationAdd BLAST36
Repeati362 – 397Pumilio 61 PublicationAdd BLAST36
Repeati398 – 435Pumilio 71 PublicationAdd BLAST38
Repeati436 – 504Pumilio 81 PublicationAdd BLAST69
Repeati505 – 551Pumilio 91 PublicationAdd BLAST47
Repeati552 – 596Pumilio 101 PublicationAdd BLAST45
Repeati597 – 636Pumilio 111 PublicationAdd BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni289 – 297HA-89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi106 – 118Nuclear localization signal1 PublicationAdd BLAST13

Domaini

The HA-8 region can be cleaved and exposed at the cell surface where it plays a role as a minor histocompatibility HLA-A*0201-restricted antigen.
A 90 degree bend between Pumilio repeats 3 and 4 gives rise to a L-shaped protein.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2050 Eukaryota
ENOG410XSHY LUCA
GeneTreeiENSGT00390000015757
HOVERGENiHBG052166
InParanoidiQ15397
KOiK14844
OMAiREWYGPE
OrthoDBiEOG091G06QP
PhylomeDBiQ15397
TreeFamiTF312954

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR012959 CPL_dom
IPR033133 PUM-HD
IPR001313 Pumilio_RNA-bd_rpt
PfamiView protein in Pfam
PF08144 CPL, 1 hit
SMARTiView protein in SMART
SM00025 Pumilio, 6 hits
SUPFAMiSSF48371 SSF48371, 2 hits
PROSITEiView protein in PROSITE
PS50302 PUM, 5 hits
PS50303 PUM_HD, 1 hit

Sequencei

Sequence statusi: Complete.

Q15397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKGKKQFT GKSTKTAQEK NRFHKNSDSG SSKTFPTRKV AKEGGPKVTS
60 70 80 90 100
RNFEKSITKL GKKGVKQFKN KQQGDKSPKN KFQPANKFNK KRKFQPDGRS
110 120 130 140 150
DESAAKKPKW DDFKKKKKEL KQSRQLSDKT NYDIVVRAKQ MWEILRRKDC
160 170 180 190 200
DKEKRVKLMS DLQKLIQGKI KTIAFAHDST RVIQCYIQYG NEEQRKQAFE
210 220 230 240 250
ELRDDLVELS KAKYSRNIVK KFLMYGSKPQ IAEIIRSFKG HVRKMLRHAE
260 270 280 290 300
ASAIVEYAYN DKAILEQRNM LTEELYGNTF QLYKSADHRT LDKVLEVQPE
310 320 330 340 350
KLELIMDEMK QILTPMAQKE AVIKHSLVHK VFLDFFTYAP PKLRSEMIEA
360 370 380 390 400
IREAVVYLAH THDGARVAMH CLWHGTPKDR KVIVKTMKTY VEKVANGQYS
410 420 430 440 450
HLVLLAAFDC IDDTKLVKQI IISEIISSLP SIVNDKYGRK VLLYLLSPRD
460 470 480 490 500
PAHTVREIIE VLQKGDGNAH SKKDTEVRRR ELLESISPAL LSYLQEHAQE
510 520 530 540 550
VVLDKSACVL VSDILGSATG DVQPTMNAIA SLAATGLHPG GKDGELHIAE
560 570 580 590 600
HPAGHLVLKW LIEQDKKMKE NGREGCFAKT LVEHVGMKNL KSWASVNRGA
610 620 630 640
IILSSLLQSC DLEVANKVKA ALKSLIPTLE KTKSTSKGIE ILLEKLST
Length:648
Mass (Da):73,584
Last modified:November 8, 2005 - v3
Checksum:i449BFBF22F1BBA86
GO

Sequence cautioni

The sequence BAA02808 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI12924 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAI15123 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti625L → S in CAG33238 (Ref. 8) Curated1

Polymorphismi

The following alleles of HA-8 are known: HA-8R, HA-8P, HA-8PL, of which only HA-8R leads to specific cytotoxic T lymphocyte (CTL) recognition. The lack of CTL recognition of cells expressing HA-8P may be due to impaired transport associated with antigen processing. The sequence shown is that of HA-8R.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05161313S → N1 PublicationCorresponds to variant dbSNP:rs10968457Ensembl.1
Natural variantiVAR_051614264I → V. Corresponds to variant dbSNP:rs35869387Ensembl.1
Natural variantiVAR_023772289R → P in allele HA-8P and allele HA-8PL. 2 PublicationsCorresponds to variant dbSNP:rs2173904Ensembl.1
Natural variantiVAR_023773297V → L in allele HA-8PL. 2 PublicationsCorresponds to variant dbSNP:rs2270891Ensembl.1
Natural variantiVAR_051615414T → S. Corresponds to variant dbSNP:rs3736390Ensembl.1
Natural variantiVAR_051616480R → Q. Corresponds to variant dbSNP:rs2270889Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK292169 mRNA Translation: BAF84858.1
AL354723, AL589675 Genomic DNA Translation: CAI15123.2 Sequence problems.
AL589675, AL354723 Genomic DNA Translation: CAI12924.2 Sequence problems.
CH471071 Genomic DNA Translation: EAW58802.1
BC016137 mRNA Translation: AAH16137.2
D13645 mRNA Translation: BAA02808.1 Different initiation.
AF490254 mRNA Translation: AAO85462.1
CR456957 mRNA Translation: CAG33238.1
AY047588 mRNA Translation: AAL06072.1
AY047589 mRNA Translation: AAL06073.1
AY047590 mRNA Translation: AAL06074.1
CCDSiCCDS6448.2
RefSeqiNP_055693.4, NM_014878.4
UniGeneiHs.493309

Genome annotation databases

EnsembliENST00000397885; ENSP00000380982; ENSG00000080608
GeneIDi9933
KEGGihsa:9933
UCSCiuc003zhp.2 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPUM3_HUMAN
AccessioniPrimary (citable) accession number: Q15397
Secondary accession number(s): A8K804
, Q547G7, Q5SZY9, Q6IB47, Q96B27, Q96L78, Q96L79, Q96L80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 8, 2005
Last modified: June 20, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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