Q15388 (TOM20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import receptor subunit TOM20 homolog

Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Outer mitochondrial membrane receptor Tom20

Gene names

Name:	TOMM20
Synonyms:	KIAA0016

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore By similarity.
Subunit structure	Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1. Ref.14
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the Tom20 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein targeting to mitochondrion Inferred from direct assay. Source: HGNC
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane translocase complex Non-traceable author statement Ref.2. Source: UniProtKB
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from direct assay. Source: HGNC unfolded protein binding Inferred from direct assay. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Mitochondrial import receptor subunit TOM20 homolog		PRO_0000051538
Regions
	Topological domain	1 – 6	6	Mitochondrial intermembrane Potential
	Transmembrane	7 – 24	18	Helical; Potential
	Topological domain	25 – 145	121	Cytoplasmic Potential
Amino acid modifications
	Modified residue	135	1	Phosphoserine Ref.13
	Modified residue	138	1	Phosphoserine Ref.10 Ref.12 Ref.13
Natural variations
	Natural variant	117	1	P → L. Corresponds to variant rs16991984 [ dbSNP | Ensembl ].		VAR_052366
	Natural variant	134	1	V → L. Corresponds to variant rs1049510 [ dbSNP | Ensembl ].		VAR_052367
Experimental info
	Sequence conflict	145	1	E → D in CAG32999. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q15388 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5153BD25AC5D9B3A	FASTA	145	16,298
	10 20 30 40 50 60 MVGRNSAIAA GVCGALFIGY CIYFDRKRRS DPNFKNRLRE RRKKQKLAKE RAGLSKLPDL 70 80 90 100 110 120 KDAEAVQKFF LEEIQLGEEL LAQGEYEKGV DHLTNAIAVC GQPQQLLQVL QQTLPPPVFQ 130 140 MLLTKLPTIS QRIVSAQSLA EDDVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the human mitochondrial protein import receptor, huMas20p. Complementation of delta mas20 in yeast." Goping I.S., Millar D.G., Shore G.C. FEBS Lett. 373:45-50(1995) [PubMed: 7589431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fibroblast.
[2]	"A human homolog of the mitochondrial protein import receptor Mom19 can assemble with the yeast mitochondrial receptor complex." Seki N., Moczko M., Nagase T., Zufall N., Ehmann B., Dietmeier K., Schaefer E., Nomura N., Pfanner N. FEBS Lett. 375:307-310(1995) [PubMed: 7498524] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Gene structure of the human mitochondrial outer membrane receptor tom20 and evolutionary study of its family of processed pseudogenes." Hernandez J.M., Giner P., Hernandez-Yago J. Gene 239:283-291(1999) [PubMed: 10548729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Cervix, Eye and Pancreas.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Identification of Tom5 and Tom6 in the preprotein translocase complex of human mitochondrial outer membrane." Kato H., Mihara K. Biochem. Biophys. Res. Commun. 369:958-963(2008) [PubMed: 18331822] [Abstract] Cited for: IDENTIFICATION IN TOM COMPLEX.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 AND SER-138, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[14]	"Identification and characterization of mitochondrial targeting sequence of human apurinic/apyrimidinic endonuclease 1." Li M., Zhong Z., Zhu J., Xiang D., Dai N., Cao X., Qing Y., Yang Z., Xie J., Li Z., Baugh L., Wang G., Wang D. J. Biol. Chem. 285:14871-14881(2010) [PubMed: 20231292] [Abstract] Cited for: INTERACTION WITH APEX1.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF126962 , AF126958, AF126959, AF126960, AF126961 Genomic DNA. Translation: AAF13354.1. D13641 mRNA. Translation: BAA02804.1. AK289810 mRNA. Translation: BAF82499.1. CR456718 mRNA. Translation: CAG32999.1. AL732292 Genomic DNA. Translation: CAI21937.1. CH471098 Genomic DNA. Translation: EAW70003.1. BC000882 mRNA. Translation: AAH00882.1. BC066335 mRNA. Translation: AAH66335.1. BC071994 mRNA. Translation: AAH71994.1. BC100286 mRNA. Translation: AAI00287.1. BC107851 mRNA. Translation: AAI07852.1.
IPI	IPI00016676.
PIR	S68215.
RefSeq	NP_055580.1. NM_014765.2.
UniGene	Hs.533192.
3D structure databases
ProteinModelPortal	Q15388.
SMR	Q15388. Positions 59-126.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15388. 10 interactions.
MINT	MINT-1403841.
STRING	Q15388.
PTM databases
PhosphoSite	Q15388.
Polymorphism databases
DMDM	2498697.
Proteomic databases
PeptideAtlas	Q15388.
PRIDE	Q15388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000366607; ENSP00000355566; ENSG00000173726.
GeneID	9804.
KEGG	hsa:9804.
UCSC	uc001hwl.1. human.
Organism-specific databases
CTD	9804.
GeneCards	GC01M235272.
H-InvDB	HIX0001711.
HGNC	HGNC:20947. TOMM20.
HPA	CAB005585. HPA011562.
MIM	601848. gene.
neXtProt	NX_Q15388.
PharmGKB	PA134964372.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG20020.
GeneTree	ENSGT00390000011698.
HOGENOM	HBG444091.
HOVERGEN	HBG057315.
InParanoid	Q15388.
OMA	RFFLQEV.
OrthoDB	EOG48SGVH.
PhylomeDB	Q15388.
Gene expression databases
ArrayExpress	Q15388.
Bgee	Q15388.
CleanEx	HS_TOMM20.
Genevestigator	Q15388.
GermOnline	ENSG00000173726. Homo sapiens.
Family and domain databases
InterPro	IPR002056. MAS20_rcpt-related. IPR022422. MAS20_rcpt_metazoan. IPR023392. Tom20_dom. [Graphical view]
Gene3D	G3DSA:1.20.960.10. G3DSA:1.20.960.10. 1 hit.
PANTHER	PTHR12430. MAS20_rcpt. 1 hit.
Pfam	PF02064. MAS20. 1 hit. [Graphical view]
PIRSF	PIRSF037707. MAS20_rcpt. 1 hit.
PRINTS	PR01989. EUOM20RECPTR. PR00351. OM20RECEPTOR.
SUPFAM	SSF47157. MAS20_rcpt. 1 hit.
TIGRFAMs	TIGR00985. 3a0801s04tom. 1 hit.
ProtoNet	Search...
Other
NextBio	36912.
SOURCE	Search...

Entry information

Entry name

TOM20_HUMAN

Accession

Primary (citable) accession number: Q15388
Secondary accession number(s): A8K195, Q498B3, Q6IBT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.