Reviewed,
UniProtKB/Swiss-Prot Q15370 (ELOB_HUMAN)
Last modified
November 25, 2008.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Transcription elongation factor B polypeptide 2 Alternative name(s): RNA polymerase II transcription factor SIII subunit B SIII p18 Elongin B Short name=EloB Elongin 18 kDa subunit | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 118 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. |
| Pathway | |
| Subunit structure | Heterotrimer of an A (A1, A2 or A3), B and C subunit. Part of E3 ubiquitin ligase complexes with CUL5 or CUL2, RBX1 and a substrate adapter protein that can be either SOCS1, TCEB3, VHL or WSB1 By similarity. Substrate adapter protein can be a viral protein such as HIV Vif. Interacts with VHL. |
| Subcellular location | NucleusProbable. |
| Sequence similarities | Contains 1 ubiquitin-like domain. |
| Sequence caution | The sequence AAC08452.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation Ubl conjugation pathway |
| Cellular component | Nucleus |
| PTM | Acetylation |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | RNA elongation from RNA polymerase II promoter Traceable author statement. Source: ProtInc protein complex assemblyTraceable author statement. Source: ProtInc protein modification processInferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytosol Inferred from Experiment. Source: Reactome nucleoplasmInferred from Experiment. Source: Reactome |
| Molecular function | protein binding Inferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 118 | 118 | Transcription elongation factor B polypeptide 2 | PRO_0000114914 | ||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||
| Domain | 1 – 66 | 66 | Ubiquitin-like | |||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||
| Modified residue | 1 | 1 | N-acetylmethionine By similarity | |||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||
| Beta strand | 2 – 10 | 9 | ||||||||||||||||||||||||||
| Beta strand | 12 – 19 | 8 | ||||||||||||||||||||||||||
| Helix | 24 – 35 | 12 | ||||||||||||||||||||||||||
| Helix | 39 – 41 | 3 | ||||||||||||||||||||||||||
| Beta strand | 42 – 46 | 5 | ||||||||||||||||||||||||||
| Turn | 57 – 61 | 5 | ||||||||||||||||||||||||||
| Turn | 64 – 66 | 3 | ||||||||||||||||||||||||||
| Beta strand | 73 – 79 | 7 | ||||||||||||||||||||||||||
| Beta strand | 82 – 84 | 3 | ||||||||||||||||||||||||||
| Helix | 101 – 103 | 3 | ||||||||||||||||||||||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Positive regulation of general transcription factor SIII by a tailed ubiquitin homolog." Garrett K.P., Aso T., Bradsher J.N., Foundling S.I., Lane W.S., Conaway R.C., Conaway J.W. Proc. Natl. Acad. Sci. U.S.A. 92:7172-7176(1995) [PubMed: 7638163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. |
| [2] | "The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. Pennacchio L.A.Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Placenta. |
| [4] | "Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity." Aso T., Yamazaki K., Aigaki T., Kitajima S. Biochem. Biophys. Res. Commun. 276:355-361(2000) [PubMed: 11006129] [Abstract] Cited for: INTERACTION WITH VHL. |
| [5] | "Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation." Mehle A., Goncalves J., Santa-Marta M., McPike M., Gabuzda D. Genes Dev. 18:2861-2866(2004) [PubMed: 15574592] [Abstract] Cited for: INTERACTION WITH HIV VIF. |
| [6] | "Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function." Stebbins C.E., Kaelin W.G. Jr., Pavletich N.P. Science 284:455-461(1999) [PubMed: 10205047] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH TCEB1 AND VHL. |
| [7] | "Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL." Hon W.-C., Wilson M.I., Harlos K., Claridge T.D.W., Schofield C.J., Pugh C.W., Maxwell P.H., Ratcliffe P.J., Stuart D.I., Jones E.Y. Nature 417:975-978(2002) [PubMed: 12050673] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) COMPLEX WITH TCEB1; VHL AND HIF1A. |
| [8] | "Structure of an HIF-1alpha-pVHL complex: hydroxyproline recognition in signaling." Min J.-H., Yang H., Ivan M., Gertler F., Kaelin W.G. Jr., Pavletich N.P. Science 296:1886-1889(2002) [PubMed: 12004076] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) COMPLEX WITH TCEB1; VHL AND HIF1A. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L42856 mRNA. Translation: AAA75522.1. AC004493 Genomic DNA. Translation: AAC08452.1. Sequence problems. BC013306 mRNA. Translation: AAH13306.1. BC065000 mRNA. Translation: AAH65000.1. | |||||||||||||||||||||||||||||||||||||||||||
| PIR | I59405. | ||||||||||||||||||||||||||||||||||||||||||
| RefSeq | NP_009039.1. | ||||||||||||||||||||||||||||||||||||||||||
| UniGene | Hs.172772 | ||||||||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||||||||
| IntAct | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||||||||||||||
| PeptideAtlas | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||||||||
| Ensembl | ENSG00000103363. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||||||||||||||
| GeneID | 6923. | ||||||||||||||||||||||||||||||||||||||||||
| KEGG | hsa:6923. | ||||||||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||||||||
| H-InvDB | HIX0036589. HIX0038597. | ||||||||||||||||||||||||||||||||||||||||||
| HGNC | HGNC:11619. TCEB2. | ||||||||||||||||||||||||||||||||||||||||||
| MIM | 600787. gene. | ||||||||||||||||||||||||||||||||||||||||||
| PharmGKB | PA36378. | ||||||||||||||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||||||||
| HOGENOM | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
| HOVERGEN | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||||||||||||||
| Reactome | REACT_1788. Transcription. REACT_1892. Elongation arrest and recovery. REACT_6143. Pausing and recovery of Tat-mediated HIV-1 elongation. REACT_6185. HIV Infection. REACT_6244. Pausing and recovery of HIV-1 elongation. REACT_6259. HIV-1 elongation arrest and recovery. REACT_6344. Tat-mediated HIV-1 elongation arrest and recovery. REACT_71. Gene Expression. REACT_769. Pausing and recovery of elongation. | ||||||||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||||||||
| ArrayExpress | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
| CleanEx | HS_TCEB2. | ||||||||||||||||||||||||||||||||||||||||||
| GermOnline | ENSG00000103363. Homo sapiens. | ||||||||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||||||||
| InterPro | IPR000626. Ubiquitin. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| SMART | SM00213. UBQ. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PROSITE | PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||||||||
| LinkHub | Q15370. | ||||||||||||||||||||||||||||||||||||||||||
| NextBio | 27083. | ||||||||||||||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | ELOB_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15370 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 16 Human chromosome 16: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


