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Protein

Poly(rC)-binding protein 1

Gene

PCBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943).1 Publication

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • mRNA binding Source: BHF-UCL
  • RNA binding Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: Ensembl
  • sequence-specific single stranded DNA binding Source: Ensembl
  • single-stranded DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Ribonucleoprotein, RNA-binding
Biological processViral RNA replication

Enzyme and pathway databases

ReactomeiR-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(rC)-binding protein 1
Alternative name(s):
Alpha-CP1
Heterogeneous nuclear ribonucleoprotein E1
Short name:
hnRNP E1
Nucleic acid-binding protein SUB2.3
Gene namesi
Name:PCBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:8647. PCBP1.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • cytosol Source: HPA
  • extracellular exosome Source: UniProtKB
  • membrane Source: UniProtKB
  • nuclear speck Source: HPA
  • nucleoplasm Source: HPA
  • P-body Source: HPA

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5093.
OpenTargetsiENSG00000169564.
PharmGKBiPA32986.

Polymorphism and mutation databases

BioMutaiPCBP1.
DMDMi42560548.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000500871 – 356Poly(rC)-binding protein 1Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei189PhosphoserineBy similarity1
Modified residuei190PhosphoserineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei273PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated; lowers poly(rC)-binding activity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15365.
PaxDbiQ15365.
PeptideAtlasiQ15365.
PRIDEiQ15365.
TopDownProteomicsiQ15365.

2D gel databases

OGPiQ15365.
REPRODUCTION-2DPAGEiIPI00016610.
UCD-2DPAGEiQ15365.

PTM databases

iPTMnetiQ15365.
PhosphoSitePlusiQ15365.
SwissPalmiQ15365.

Expressioni

Tissue specificityi

Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

Gene expression databases

BgeeiENSG00000169564.
CleanExiHS_PCBP1.
ExpressionAtlasiQ15365. baseline and differential.
GenevisibleiQ15365. HS.

Organism-specific databases

HPAiCAB037113.
HPA043803.
HPA068497.

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111126. 171 interactors.
DIPiDIP-38136N.
IntActiQ15365. 88 interactors.
MINTiMINT-96267.
STRINGi9606.ENSP00000305556.

Structurei

Secondary structure

1356
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 21Combined sources8
Helixi22 – 29Combined sources8
Helixi31 – 33Combined sources3
Helixi34 – 43Combined sources10
Beta strandi46 – 49Combined sources4
Beta strandi55 – 64Combined sources10
Helixi65 – 81Combined sources17
Turni82 – 84Combined sources3
Beta strandi280 – 287Combined sources8
Helixi288 – 290Combined sources3
Helixi291 – 295Combined sources5
Helixi297 – 299Combined sources3
Helixi300 – 309Combined sources10
Beta strandi312 – 315Combined sources4
Beta strandi323 – 331Combined sources9
Helixi333 – 346Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WVNX-ray2.10A279-356[»]
1ZTGX-ray3.00A/B/C/D14-85[»]
3VKEX-ray1.77A/B/C/D14-86[»]
ProteinModelPortaliQ15365.
SMRiQ15365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 75KH 1PROSITE-ProRule annotationAdd BLAST63
Domaini97 – 162KH 2PROSITE-ProRule annotationAdd BLAST66
Domaini279 – 343KH 3PROSITE-ProRule annotationAdd BLAST65

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiQ15365.
KOiK12889.
OMAiTLTKLHQ.
OrthoDBiEOG091G0DHI.
PhylomeDBiQ15365.
TreeFamiTF318292.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiView protein in InterPro
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
PfamiView protein in Pfam
PF00013. KH_1. 3 hits.
SMARTiView protein in SMART
SM00322. KH. 3 hits.
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 3 hits.

Sequencei

Sequence statusi: Complete.

Q15365-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAGVTESGL NVTLTIRLLM HGKEVGSIIG KKGESVKRIR EESGARINIS
60 70 80 90 100
EGNCPERIIT LTGPTNAIFK AFAMIIDKLE EDINSSMTNS TAASRPPVTL
110 120 130 140 150
RLVVPATQCG SLIGKGGCKI KEIRESTGAQ VQVAGDMLPN STERAITIAG
160 170 180 190 200
VPQSVTECVK QICLVMLETL SQSPQGRVMT IPYQPMPASS PVICAGGQDR
210 220 230 240 250
CSDAAGYPHA THDLEGPPLD AYSIQGQHTI SPLDLAKLNQ VARQQSHFAM
260 270 280 290 300
MHGGTGFAGI DSSSPEVKGY WASLDASTQT THELTIPNNL IGCIIGRQGA
310 320 330 340 350
NINEIRQMSG AQIKIANPVE GSSGRQVTIT GSAASISLAQ YLINARLSSE

KGMGCS
Length:356
Mass (Da):37,498
Last modified:February 16, 2004 - v2
Checksum:i6D1A261276CA206D
GO

Sequence cautioni

The sequence CAA82631 differs from that shown. Reason: Frameshift at position 301.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti205A → V in CAA55016 (PubMed:7607214).Curated1
Sequence conflicti299 – 300Missing in CAA82631 (PubMed:8152927).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78137 mRNA. Translation: CAA55016.1.
U24223 mRNA. Translation: AAA91317.1.
Z29505 mRNA. Translation: CAA82631.1. Frameshift.
BC039742 mRNA. Translation: AAH39742.1.
CCDSiCCDS1898.1.
RefSeqiNP_006187.2. NM_006196.3.
UniGeneiHs.2853.

Genome annotation databases

EnsembliENST00000303577; ENSP00000305556; ENSG00000169564.
GeneIDi5093.
KEGGihsa:5093.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPCBP1_HUMAN
AccessioniPrimary (citable) accession number: Q15365
Secondary accession number(s): Q13157, Q14975
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 16, 2004
Last modified: July 5, 2017
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references