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Protein

Transcription factor E2F5

Gene

E2F5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi47 – 118Sequence analysisAdd BLAST72

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: ProtInc
  • transcription factor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cilium biogenesis/degradation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133740-MONOMER.
ReactomeiR-HSA-1538133. G0 and Early G1.
R-HSA-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-HSA-69231. Cyclin D associated events in G1.
SignaLinkiQ15329.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F5
Short name:
E2F-5
Gene namesi
Name:E2F5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:3119. E2F5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1875.
OpenTargetsiENSG00000133740.
PharmGKBiPA27577.

Polymorphism and mutation databases

BioMutaiE2F5.
DMDMi2494230.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194691 – 346Transcription factor E2F5Add BLAST346

Proteomic databases

MaxQBiQ15329.
PaxDbiQ15329.
PeptideAtlasiQ15329.
PRIDEiQ15329.

PTM databases

iPTMnetiQ15329.
PhosphoSitePlusiQ15329.

Expressioni

Gene expression databases

BgeeiENSG00000133740.
CleanExiHS_E2F5.
ExpressionAtlasiQ15329. baseline and differential.
GenevisibleiQ15329. HS.

Organism-specific databases

HPAiHPA055723.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.3 Publications

GO - Molecular functioni

  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi108207. 9 interactors.
DIPiDIP-24229N.
IntActiQ15329. 8 interactors.
STRINGi9606.ENSP00000398124.

Structurei

3D structure databases

ProteinModelPortaliQ15329.
SMRiQ15329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 98Leucine-zipperAdd BLAST23
Regioni119 – 215DimerizationSequence analysisAdd BLAST97
Regioni287 – 346TransactivationSequence analysisAdd BLAST60
Regioni323 – 340RBL2 associationSequence analysisAdd BLAST18

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 118DEF boxAdd BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 39Poly-Pro7
Compositional biasi233 – 236Poly-Ser4

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ15329.
KOiK04682.
OMAiLFDVQCS.
OrthoDBiEOG091G0CJQ.
PhylomeDBiQ15329.
TreeFamiTF105566.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR028316. E2F5.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15329-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR
60 70 80 90 100
HEKSLGLLTT KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG
110 120 130 140 150
IDLIEKKSKN SIQWKGVGAG CNTKEVIDRL RYLKAEIEDL ELKERELDQQ
160 170 180 190 200
KLWLQQSIKN VMDDSINNRF SYVTHEDICN CFNGDTLLAI QAPSGTQLEV
210 220 230 240 250
PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT
260 270 280 290 300
QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSAGSISG
310 320 330 340
DIIDELMSSD VFPLLRLSPT PADDYNFNLD DNEGVCDLFD VQILNY
Length:346
Mass (Da):37,610
Last modified:November 1, 1996 - v1
Checksum:iF1408A755E67D879
GO
Isoform 2 (identifier: Q15329-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     295-295: Missing.

Show »
Length:345
Mass (Da):37,539
Checksum:i0CBCD335800F4863
GO
Isoform 3 (identifier: Q15329-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Note: No experimental confirmation available.
Show »
Length:185
Mass (Da):20,192
Checksum:iAECF4C7A7000F8BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153W → L in CAB01634 (PubMed:9464260).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01434818G → A.1 PublicationCorresponds to variant rs4150841dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0446601 – 161Missing in isoform 3. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_040098295Missing in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31556 mRNA. Translation: AAB00179.1.
X86097 mRNA. Translation: CAA60051.1.
U15642 mRNA. Translation: AAC50120.1.
Z78409 mRNA. Translation: CAB01634.1.
AY162833 Genomic DNA. Translation: AAN46737.1.
AL583354 mRNA. No translation available.
AC011773 Genomic DNA. No translation available.
CCDSiCCDS47885.1. [Q15329-1]
CCDS47886.1. [Q15329-2]
CCDS55254.1. [Q15329-3]
PIRiJC5833.
RefSeqiNP_001077057.1. NM_001083588.1. [Q15329-2]
NP_001077058.1. NM_001083589.1. [Q15329-3]
NP_001942.2. NM_001951.3. [Q15329-1]
UniGeneiHs.445758.

Genome annotation databases

EnsembliENST00000416274; ENSP00000398124; ENSG00000133740. [Q15329-1]
ENST00000418930; ENSP00000414312; ENSG00000133740. [Q15329-2]
ENST00000517476; ENSP00000429120; ENSG00000133740. [Q15329-3]
GeneIDi1875.
KEGGihsa:1875.
UCSCiuc003ycz.6. human. [Q15329-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31556 mRNA. Translation: AAB00179.1.
X86097 mRNA. Translation: CAA60051.1.
U15642 mRNA. Translation: AAC50120.1.
Z78409 mRNA. Translation: CAB01634.1.
AY162833 Genomic DNA. Translation: AAN46737.1.
AL583354 mRNA. No translation available.
AC011773 Genomic DNA. No translation available.
CCDSiCCDS47885.1. [Q15329-1]
CCDS47886.1. [Q15329-2]
CCDS55254.1. [Q15329-3]
PIRiJC5833.
RefSeqiNP_001077057.1. NM_001083588.1. [Q15329-2]
NP_001077058.1. NM_001083589.1. [Q15329-3]
NP_001942.2. NM_001951.3. [Q15329-1]
UniGeneiHs.445758.

3D structure databases

ProteinModelPortaliQ15329.
SMRiQ15329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108207. 9 interactors.
DIPiDIP-24229N.
IntActiQ15329. 8 interactors.
STRINGi9606.ENSP00000398124.

PTM databases

iPTMnetiQ15329.
PhosphoSitePlusiQ15329.

Polymorphism and mutation databases

BioMutaiE2F5.
DMDMi2494230.

Proteomic databases

MaxQBiQ15329.
PaxDbiQ15329.
PeptideAtlasiQ15329.
PRIDEiQ15329.

Protocols and materials databases

DNASUi1875.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000416274; ENSP00000398124; ENSG00000133740. [Q15329-1]
ENST00000418930; ENSP00000414312; ENSG00000133740. [Q15329-2]
ENST00000517476; ENSP00000429120; ENSG00000133740. [Q15329-3]
GeneIDi1875.
KEGGihsa:1875.
UCSCiuc003ycz.6. human. [Q15329-1]

Organism-specific databases

CTDi1875.
DisGeNETi1875.
GeneCardsiE2F5.
HGNCiHGNC:3119. E2F5.
HPAiHPA055723.
MIMi600967. gene.
neXtProtiNX_Q15329.
OpenTargetsiENSG00000133740.
PharmGKBiPA27577.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2577. Eukaryota.
ENOG410XNYI. LUCA.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ15329.
KOiK04682.
OMAiLFDVQCS.
OrthoDBiEOG091G0CJQ.
PhylomeDBiQ15329.
TreeFamiTF105566.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000133740-MONOMER.
ReactomeiR-HSA-1538133. G0 and Early G1.
R-HSA-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-HSA-69231. Cyclin D associated events in G1.
SignaLinkiQ15329.

Miscellaneous databases

ChiTaRSiE2F5. human.
GeneWikiiE2F5.
GenomeRNAii1875.
PROiQ15329.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133740.
CleanExiHS_E2F5.
ExpressionAtlasiQ15329. baseline and differential.
GenevisibleiQ15329. HS.

Family and domain databases

CDDicd14660. E2F_DD. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR028316. E2F5.
IPR032198. E2F_CC-MB.
IPR003316. E2F_WHTH_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
PfamiPF16421. E2F_CC-MB. 1 hit.
PF02319. E2F_TDP. 1 hit.
[Graphical view]
SMARTiSM01372. E2F_TDP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE2F5_HUMAN
AccessioniPrimary (citable) accession number: Q15329
Secondary accession number(s): E9PBN9, Q16601, Q92756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.