Q15329 (E2F5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor E2F5
Short name=E2F-5

Gene names

Name:

E2F5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation.
Subunit structure	Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Ref.9
Subcellular location	Nucleus.
Sequence similarities	Belongs to the E2F/DP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G1 phase of mitotic cell cycle Traceable author statement. Source: Reactome organ morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Traceable author statement. Source: Reactome transforming growth factor beta receptor signaling pathway Traceable author statement. Source: Reactome
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleoplasm Traceable author statement. Source: Reactome transcription factor complex Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15329-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15329-2)

The sequence of this isoform differs from the canonical sequence as follows:
     295-295: Missing.

Isoform 3 (identifier: Q15329-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Transcription factor E2F5		PRO_0000219469
Regions
	DNA binding	47 – 118	72	Potential
	Region	76 – 98	23	Leucine-zipper
	Region	119 – 215	97	Dimerization Potential
	Region	287 – 346	60	Transactivation Potential
	Region	323 – 340	18	RBL2 association Potential
	Motif	81 – 118	38	DEF box
	Compositional bias	33 – 39	7	Poly-Pro
	Compositional bias	233 – 236	4	Poly-Ser
Natural variations
	Alternative sequence	1 – 161	161	Missing in isoform 3.		VSP_044660
	Alternative sequence	295	1	Missing in isoform 2.		VSP_040098
	Natural variant	18	1	G → A. Ref.5 Corresponds to variant rs4150841 [ dbSNP | Ensembl ].		VAR_014348
Experimental info
	Sequence conflict	153	1	W → L in CAB01634. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F1408A755E67D879	FASTA	346	37,610
	10 20 30 40 50 60 MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR HEKSLGLLTT 70 80 90 100 110 120 KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG 130 140 150 160 170 180 CNTKEVIDRL RYLKAEIEDL ELKERELDQQ KLWLQQSIKN VMDDSINNRF SYVTHEDICN 190 200 210 220 230 240 CFNGDTLLAI QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV 250 260 270 280 290 300 FPVPPPDDLT QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSAGSISG 310 320 330 340 DIIDELMSSD VFPLLRLSPT PADDYNFNLD DNEGVCDLFD VQILNY
	Isoform 2 [UniParc]. Checksum: 0CBCD335800F4863	FASTA	345	37,539
	10 20 30 40 50 60 MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR HEKSLGLLTT 70 80 90 100 110 120 KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG 130 140 150 160 170 180 CNTKEVIDRL RYLKAEIEDL ELKERELDQQ KLWLQQSIKN VMDDSINNRF SYVTHEDICN 190 200 210 220 230 240 CFNGDTLLAI QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV 250 260 270 280 290 300 FPVPPPDDLT QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSGSISGD 310 320 330 340 IIDELMSSDV FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY
	Isoform 3 [UniParc]. Checksum: AECF4C7A7000F8BD	FASTA	185	20,192
	10 20 30 40 50 60 MDDSINNRFS YVTHEDICNC FNGDTLLAIQ APSGTQLEVP IPEMGQNGQK KYQINLKSHS 70 80 90 100 110 120 GPIHVLLINK ESSSSKPVVF PVPPPDDLTQ PSSQSLTPVT PQKSSMATQN LPEQHVSERS 130 140 150 160 170 180 QALQQTSATD ISSAGSISGD IIDELMSSDV FPLLRLSPTP ADDYNFNLDD NEGVCDLFDV QILNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural characterization and specificity of expression of E2F-5: a new member of the E2F family of transcription factors." Itoh A., Levinson S.F., Morita T., Kourembanas S., Brody J.S., Mitsialis S.A. Cell. Mol. Biol. Res. 41:147-154(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Fetal lung and Placenta.
[2]	"E2F-5, a new E2F family member that interacts with p130 in vivo." Hijmans E.M., Voorhoeve P.M., Beijersbergen R.L., van 't Veer L.J., Bernards R. Mol. Cell. Biol. 15:3082-3089(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH RBL2. Tissue: Colon carcinoma.
[3]	"E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle." Sardet C., Vidal M., Cobrinik D., Geng Y., Onufryk C., Chen A., Weinberg R.A. Proc. Natl. Acad. Sci. U.S.A. 92:2403-2407(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"The molecular and functional characterization of E2F-5 transcription factor." Vaishnav Y.N., Vaishnav M.Y., Pant V. Biochem. Biophys. Res. Commun. 242:586-592(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[5]	NIEHS SNPs program Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-18.
[6]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: B-cell.
[7]	"DNA sequence and analysis of human chromosome 8." Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T. , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S. Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release." Rubin S.M., Gall A.-L., Zheng N., Pavletich N.P. Cell 123:1093-1106(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH WITH RB1 AND TFDP1.
[9]	"Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence." Litovchick L., Sadasivam S., Florens L., Zhu X., Swanson S.K., Velmurugan S., Chen R., Washburn M.P., Liu X.S., DeCaprio J.A. Mol. Cell 26:539-551(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE DREAM COMPLEX.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31556 mRNA. Translation: AAB00179.1. X86097 mRNA. Translation: CAA60051.1. U15642 mRNA. Translation: AAC50120.1. Z78409 mRNA. Translation: CAB01634.1. AY162833 Genomic DNA. Translation: AAN46737.1. AL583354 mRNA. No translation available. AC011773 Genomic DNA. No translation available.
IPI	IPI00795388. IPI00845238. IPI00940123.
PIR	JC5833.
RefSeq	NP_001077057.1. NM_001083588.1. NP_001077058.1. NM_001083589.1. NP_001942.2. NM_001951.3.
UniGene	Hs.445758.
3D structure databases
ProteinModelPortal	Q15329.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24229N.
IntAct	Q15329. 3 interactions.
STRING	9606.ENSP00000398124.
PTM databases
PhosphoSite	Q15329.
Polymorphism databases
DMDM	2494230.
Proteomic databases
PaxDb	Q15329.
PRIDE	Q15329.
Protocols and materials databases
DNASU	1875.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000416274; ENSP00000398124; ENSG00000133740. ENST00000418930; ENSP00000414312; ENSG00000133740. ENST00000517476; ENSP00000429120; ENSG00000133740.
GeneID	1875.
KEGG	hsa:1875.
UCSC	uc003ycz.4. human. uc003yda.4. human.
Organism-specific databases
CTD	1875.
GeneCards	GC08P086089.
HGNC	HGNC:3119. E2F5.
MIM	600967. gene.
neXtProt	NX_Q15329.
PharmGKB	PA27577.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG289227.
HOGENOM	HOG000232045.
HOVERGEN	HBG002227.
InParanoid	Q15329.
KO	K04682.
Enzyme and pathway databases
Pathway_Interaction_DB	smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling.
Reactome	REACT_111102. Signal Transduction. REACT_115566. Cell Cycle. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q15329.
Bgee	Q15329.
CleanEx	HS_E2F5.
Genevestigator	Q15329.
GermOnline	ENSG00000133740. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR015633. E2F. IPR003316. E2F_TDP. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR12081. PTHR12081. 1 hit.
Pfam	PF02319. E2F_TDP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	E2F5. human.
GenomeRNAi	1875.
NextBio	7663.
SOURCE	Search...

Entry information

Entry name

E2F5_HUMAN

Accession

Primary (citable) accession number: Q15329
Secondary accession number(s): E9PBN9, Q16601, Q92756

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.