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Q15329

- E2F5_HUMAN

UniProt

Q15329 - E2F5_HUMAN

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Protein

Transcription factor E2F5

Gene
E2F5
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi47 – 11872 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. protein binding Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: ProtInc
  4. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. gene expression Source: Reactome
  2. mitotic cell cycle Source: Reactome
  3. organ morphogenesis Source: Ensembl
  4. positive regulation of transcription from RNA polymerase II promoter Source: Reactome
  5. regulation of cell cycle Source: InterPro
  6. transcription, DNA-templated Source: Reactome
  7. transcription initiation from RNA polymerase II promoter Source: Reactome
  8. transforming growth factor beta receptor signaling pathway Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_111214. G0 and Early G1.
REACT_120734. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_821. Cyclin D associated events in G1.
SignaLinkiQ15329.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F5
Short name:
E2F-5
Gene namesi
Name:E2F5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:3119. E2F5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleoplasm Source: Reactome
  3. transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27577.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Transcription factor E2F5PRO_0000219469Add
BLAST

Proteomic databases

MaxQBiQ15329.
PaxDbiQ15329.
PRIDEiQ15329.

PTM databases

PhosphoSiteiQ15329.

Expressioni

Gene expression databases

ArrayExpressiQ15329.
BgeeiQ15329.
CleanExiHS_E2F5.
GenevestigatoriQ15329.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.1 Publication

Protein-protein interaction databases

BioGridi108207. 6 interactions.
DIPiDIP-24229N.
IntActiQ15329. 4 interactions.
STRINGi9606.ENSP00000398124.

Structurei

3D structure databases

ProteinModelPortaliQ15329.
SMRiQ15329. Positions 49-115.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 9823Leucine-zipperAdd
BLAST
Regioni119 – 21597Dimerization Reviewed predictionAdd
BLAST
Regioni287 – 34660Transactivation Reviewed predictionAdd
BLAST
Regioni323 – 34018RBL2 association Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 11838DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi33 – 397Poly-Pro
Compositional biasi233 – 2364Poly-Ser

Sequence similaritiesi

Belongs to the E2F/DP family.

Phylogenomic databases

eggNOGiNOG289227.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiQ15329.
KOiK04682.
OrthoDBiEOG7WHHB1.
PhylomeDBiQ15329.
TreeFamiTF105566.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR028316. E2F5.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q15329-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR    50
HEKSLGLLTT KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG 100
IDLIEKKSKN SIQWKGVGAG CNTKEVIDRL RYLKAEIEDL ELKERELDQQ 150
KLWLQQSIKN VMDDSINNRF SYVTHEDICN CFNGDTLLAI QAPSGTQLEV 200
PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV FPVPPPDDLT 250
QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSAGSISG 300
DIIDELMSSD VFPLLRLSPT PADDYNFNLD DNEGVCDLFD VQILNY 346
Length:346
Mass (Da):37,610
Last modified:November 1, 1996 - v1
Checksum:iF1408A755E67D879
GO
Isoform 2 (identifier: Q15329-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     295-295: Missing.

Show »
Length:345
Mass (Da):37,539
Checksum:i0CBCD335800F4863
GO
Isoform 3 (identifier: Q15329-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Note: No experimental confirmation available.

Show »
Length:185
Mass (Da):20,192
Checksum:iAECF4C7A7000F8BD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti18 – 181G → A.1 Publication
Corresponds to variant rs4150841 [ dbSNP | Ensembl ].
VAR_014348

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 161161Missing in isoform 3. VSP_044660Add
BLAST
Alternative sequencei295 – 2951Missing in isoform 2. VSP_040098

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531W → L in CAB01634. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31556 mRNA. Translation: AAB00179.1.
X86097 mRNA. Translation: CAA60051.1.
U15642 mRNA. Translation: AAC50120.1.
Z78409 mRNA. Translation: CAB01634.1.
AY162833 Genomic DNA. Translation: AAN46737.1.
AL583354 mRNA. No translation available.
AC011773 Genomic DNA. No translation available.
CCDSiCCDS47885.1. [Q15329-1]
CCDS47886.1. [Q15329-2]
CCDS55254.1. [Q15329-3]
PIRiJC5833.
RefSeqiNP_001077057.1. NM_001083588.1. [Q15329-2]
NP_001077058.1. NM_001083589.1. [Q15329-3]
NP_001942.2. NM_001951.3. [Q15329-1]
XP_006716497.1. XM_006716434.1. [Q15329-3]
UniGeneiHs.445758.

Genome annotation databases

EnsembliENST00000416274; ENSP00000398124; ENSG00000133740. [Q15329-1]
ENST00000418930; ENSP00000414312; ENSG00000133740. [Q15329-2]
ENST00000517476; ENSP00000429120; ENSG00000133740. [Q15329-3]
GeneIDi1875.
KEGGihsa:1875.
UCSCiuc003ycz.4. human. [Q15329-1]
uc003yda.4. human. [Q15329-2]

Polymorphism databases

DMDMi2494230.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31556 mRNA. Translation: AAB00179.1 .
X86097 mRNA. Translation: CAA60051.1 .
U15642 mRNA. Translation: AAC50120.1 .
Z78409 mRNA. Translation: CAB01634.1 .
AY162833 Genomic DNA. Translation: AAN46737.1 .
AL583354 mRNA. No translation available.
AC011773 Genomic DNA. No translation available.
CCDSi CCDS47885.1. [Q15329-1 ]
CCDS47886.1. [Q15329-2 ]
CCDS55254.1. [Q15329-3 ]
PIRi JC5833.
RefSeqi NP_001077057.1. NM_001083588.1. [Q15329-2 ]
NP_001077058.1. NM_001083589.1. [Q15329-3 ]
NP_001942.2. NM_001951.3. [Q15329-1 ]
XP_006716497.1. XM_006716434.1. [Q15329-3 ]
UniGenei Hs.445758.

3D structure databases

ProteinModelPortali Q15329.
SMRi Q15329. Positions 49-115.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 108207. 6 interactions.
DIPi DIP-24229N.
IntActi Q15329. 4 interactions.
STRINGi 9606.ENSP00000398124.

PTM databases

PhosphoSitei Q15329.

Polymorphism databases

DMDMi 2494230.

Proteomic databases

MaxQBi Q15329.
PaxDbi Q15329.
PRIDEi Q15329.

Protocols and materials databases

DNASUi 1875.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000416274 ; ENSP00000398124 ; ENSG00000133740 . [Q15329-1 ]
ENST00000418930 ; ENSP00000414312 ; ENSG00000133740 . [Q15329-2 ]
ENST00000517476 ; ENSP00000429120 ; ENSG00000133740 . [Q15329-3 ]
GeneIDi 1875.
KEGGi hsa:1875.
UCSCi uc003ycz.4. human. [Q15329-1 ]
uc003yda.4. human. [Q15329-2 ]

Organism-specific databases

CTDi 1875.
GeneCardsi GC08P086089.
HGNCi HGNC:3119. E2F5.
MIMi 600967. gene.
neXtProti NX_Q15329.
PharmGKBi PA27577.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG289227.
HOGENOMi HOG000232045.
HOVERGENi HBG002227.
InParanoidi Q15329.
KOi K04682.
OrthoDBi EOG7WHHB1.
PhylomeDBi Q15329.
TreeFami TF105566.

Enzyme and pathway databases

Reactomei REACT_111214. G0 and Early G1.
REACT_120734. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
REACT_821. Cyclin D associated events in G1.
SignaLinki Q15329.

Miscellaneous databases

ChiTaRSi E2F5. human.
GeneWikii E2F5.
GenomeRNAii 1875.
NextBioi 7663.
PROi Q15329.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q15329.
Bgeei Q15329.
CleanExi HS_E2F5.
Genevestigatori Q15329.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR015633. E2F.
IPR028316. E2F5.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR12081. PTHR12081. 1 hit.
PTHR12081:SF35. PTHR12081:SF35. 1 hit.
Pfami PF02319. E2F_TDP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural characterization and specificity of expression of E2F-5: a new member of the E2F family of transcription factors."
    Itoh A., Levinson S.F., Morita T., Kourembanas S., Brody J.S., Mitsialis S.A.
    Cell. Mol. Biol. Res. 41:147-154(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Fetal lung and Placenta.
  2. "E2F-5, a new E2F family member that interacts with p130 in vivo."
    Hijmans E.M., Voorhoeve P.M., Beijersbergen R.L., van 't Veer L.J., Bernards R.
    Mol. Cell. Biol. 15:3082-3089(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH RBL2.
    Tissue: Colon carcinoma.
  3. "E2F-4 and E2F-5, two members of the E2F family, are expressed in the early phases of the cell cycle."
    Sardet C., Vidal M., Cobrinik D., Geng Y., Onufryk C., Chen A., Weinberg R.A.
    Proc. Natl. Acad. Sci. U.S.A. 92:2403-2407(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  4. "The molecular and functional characterization of E2F-5 transcription factor."
    Vaishnav Y.N., Vaishnav M.Y., Pant V.
    Biochem. Biophys. Res. Commun. 242:586-592(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  5. NIEHS SNPs program
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ALA-18.
  6. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: B-cell.
  7. "DNA sequence and analysis of human chromosome 8."
    Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M., Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S., Asakawa T.
    , Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A., Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III, Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K., Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P., Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H., Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B., O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K., Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L., Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G., Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W., Platzer M., Shimizu N., Lander E.S.
    Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release."
    Rubin S.M., Gall A.-L., Zheng N., Pavletich N.P.
    Cell 123:1093-1106(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WITH RB1 AND TFDP1.
  9. "Evolutionarily conserved multisubunit RBL2/p130 and E2F4 protein complex represses human cell cycle-dependent genes in quiescence."
    Litovchick L., Sadasivam S., Florens L., Zhu X., Swanson S.K., Velmurugan S., Chen R., Washburn M.P., Liu X.S., DeCaprio J.A.
    Mol. Cell 26:539-551(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE DREAM COMPLEX.

Entry informationi

Entry nameiE2F5_HUMAN
AccessioniPrimary (citable) accession number: Q15329
Secondary accession number(s): E9PBN9, Q16601, Q92756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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