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Protein

RalA-binding protein 1

Gene

RALBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.3 Publications

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATPase activity, coupled to movement of substances Source: UniProtKB
  • GTPase activator activity Source: UniProtKB
  • Rac GTPase binding Source: UniProtKB
  • Ral GTPase binding Source: UniProtKB
  • transmembrane transporter activity Source: ParkinsonsUK-UCL

GO - Biological processi

  • chemotaxis Source: ProtInc
  • endocytosis Source: GO_Central
  • positive regulation of GTPase activity Source: UniProtKB
  • regulation of GTPase activity Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: Reactome
  • signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: UniProtKB
  • transmembrane transport Source: UniProtKB
  • transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000017797-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ15311.

Protein family/group databases

TCDBi9.A.1.1.1. the non abc multidrug exporter (n-mde) family.

Names & Taxonomyi

Protein namesi
Recommended name:
RalA-binding protein 1
Short name:
RalBP1
Alternative name(s):
76 kDa Ral-interacting protein
Dinitrophenyl S-glutathione ATPase
Short name:
DNP-SG ATPase
Ral-interacting protein 1
Gene namesi
Name:RALBP1
Synonyms:RLIP1, RLIP76
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:9841. RALBP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10928.
OpenTargetsiENSG00000017797.
PharmGKBiPA34199.

Chemistry databases

DrugBankiDB00564. Carbamazepine.
DB00997. Doxorubicin.
DB00398. Sorafenib.
DB00541. Vincristine.

Polymorphism and mutation databases

BioMutaiRALBP1.
DMDMi34098413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000567332 – 655RalA-binding protein 1Add BLAST654

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei44PhosphothreonineCombined sources1
Modified residuei48PhosphoserineCombined sources1
Modified residuei62PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei93PhosphoserineCombined sources1
Modified residuei461PhosphoserineCombined sources1
Modified residuei463PhosphoserineCombined sources1
Modified residuei645PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15311.
MaxQBiQ15311.
PaxDbiQ15311.
PeptideAtlasiQ15311.
PRIDEiQ15311.

PTM databases

iPTMnetiQ15311.
PhosphoSitePlusiQ15311.

Expressioni

Tissue specificityi

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.1 Publication

Gene expression databases

BgeeiENSG00000017797.
CleanExiHS_RALBP1.
ExpressionAtlasiQ15311. baseline and differential.
GenevisibleiQ15311. HS.

Organism-specific databases

HPAiCAB046010.
HPA046651.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Q9H6F03EBI-749285,EBI-10307481
AMOTL2Q9Y2J4-43EBI-749285,EBI-10187270
C1orf216Q8TAB55EBI-749285,EBI-747505
CCDC6Q162044EBI-749285,EBI-1045350
CCL20P785562EBI-749285,EBI-3913209
CEP57Q86XR84EBI-749285,EBI-308614
GPS2Q132277EBI-749285,EBI-713355
KANK2Q63ZY34EBI-749285,EBI-2556193
PPLO604373EBI-749285,EBI-368321
SMARCE1Q969G35EBI-749285,EBI-455078
TAX1BP1Q86VP13EBI-749285,EBI-529518
TBRG1Q05BL03EBI-749285,EBI-10223693
TFPTG5E9B55EBI-749285,EBI-10178002
TNIP1Q150253EBI-749285,EBI-357849
ZNF707Q96C283EBI-749285,EBI-748111

GO - Molecular functioni

  • Rac GTPase binding Source: UniProtKB
  • Ral GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116131. 82 interactors.
IntActiQ15311. 46 interactors.
MINTiMINT-140436.
STRINGi9606.ENSP00000019317.

Structurei

Secondary structure

1655
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi184 – 187Combined sources4
Helixi194 – 200Combined sources7
Helixi211 – 222Combined sources12
Turni223 – 225Combined sources3
Turni228 – 232Combined sources5
Helixi237 – 248Combined sources12
Helixi255 – 257Combined sources3
Helixi260 – 273Combined sources14
Helixi280 – 291Combined sources12
Helixi296 – 309Combined sources14
Helixi312 – 334Combined sources23
Helixi339 – 350Combined sources12
Helixi354 – 367Combined sources14
Beta strandi384 – 386Combined sources3
Helixi395 – 414Combined sources20
Turni417 – 419Combined sources3
Helixi424 – 444Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KWHNMR-A393-446[»]
2KWINMR-B393-446[»]
2MBGNMR-A184-446[»]
ProteinModelPortaliQ15311.
SMRiQ15311.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15311.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 380Rho-GAPPROSITE-ProRule annotationAdd BLAST189

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni403 – 499Interaction with RalAAdd BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi164 – 171Poly-Lys8

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4370. Eukaryota.
ENOG410XRJ9. LUCA.
GeneTreeiENSGT00840000129752.
HOGENOMiHOG000007929.
HOVERGENiHBG044496.
InParanoidiQ15311.
KOiK08773.
OMAiYDELMEW.
OrthoDBiEOG091G01CT.
PhylomeDBiQ15311.
TreeFamiTF315411.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTECFLPPTS SPSEHRRVEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP
60 70 80 90 100
HDILHEPPDV VSDDEKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP
110 120 130 140 150
SKMKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK
160 170 180 190 200
SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ IDVPNLKPIF GIPLADAVER
210 220 230 240 250
TMMYDGIRLP AVFRECIDYV EKYGMKCEGI YRVSGIKSKV DELKAAYDRE
260 270 280 290 300
ESTNLEDYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGRTTETEKV
310 320 330 340 350
QEFQRLLKEL PECNYLLISW LIVHMDHVIA KELETKMNIQ NISIVLSPTV
360 370 380 390 400
QISNRVLYVF FTHVQELFGN VVLKQVMKPL RWSNMATMPT LPETQAGIKE
410 420 430 440 450
EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE
460 470 480 490 500
CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME
510 520 530 540 550
QFLRRQIASE KEEIERLRAE IAEIQSRQQH GRSETEEYSS ESESESEDEE
560 570 580 590 600
ELQIILEDLQ RQNEELEIKN NHLNQAIHEE REAIIELRVQ LRLLQMQRAK
610 620 630 640 650
AEQQAQEDEE PEWRGGAVQP PRDGVLEPKA AKEQPKAGKE PAKPSPSRDR

KETSI
Length:655
Mass (Da):76,063
Last modified:January 23, 2007 - v3
Checksum:iEC6F75329FD8D062
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049147617A → V.Corresponds to variant rs35867116dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42542 mRNA. Translation: AAB00103.1.
CH471113 Genomic DNA. Translation: EAX01601.1.
CH471113 Genomic DNA. Translation: EAX01602.1.
CH471113 Genomic DNA. Translation: EAX01604.1.
CH471113 Genomic DNA. Translation: EAX01605.1.
BC013126 mRNA. Translation: AAH13126.1.
CCDSiCCDS11845.1.
PIRiF59435.
RefSeqiNP_006779.1. NM_006788.3.
UniGeneiHs.528993.

Genome annotation databases

EnsembliENST00000019317; ENSP00000019317; ENSG00000017797.
ENST00000383432; ENSP00000372924; ENSG00000017797.
GeneIDi10928.
KEGGihsa:10928.
UCSCiuc002kob.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42542 mRNA. Translation: AAB00103.1.
CH471113 Genomic DNA. Translation: EAX01601.1.
CH471113 Genomic DNA. Translation: EAX01602.1.
CH471113 Genomic DNA. Translation: EAX01604.1.
CH471113 Genomic DNA. Translation: EAX01605.1.
BC013126 mRNA. Translation: AAH13126.1.
CCDSiCCDS11845.1.
PIRiF59435.
RefSeqiNP_006779.1. NM_006788.3.
UniGeneiHs.528993.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KWHNMR-A393-446[»]
2KWINMR-B393-446[»]
2MBGNMR-A184-446[»]
ProteinModelPortaliQ15311.
SMRiQ15311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116131. 82 interactors.
IntActiQ15311. 46 interactors.
MINTiMINT-140436.
STRINGi9606.ENSP00000019317.

Chemistry databases

DrugBankiDB00564. Carbamazepine.
DB00997. Doxorubicin.
DB00398. Sorafenib.
DB00541. Vincristine.

Protein family/group databases

TCDBi9.A.1.1.1. the non abc multidrug exporter (n-mde) family.

PTM databases

iPTMnetiQ15311.
PhosphoSitePlusiQ15311.

Polymorphism and mutation databases

BioMutaiRALBP1.
DMDMi34098413.

Proteomic databases

EPDiQ15311.
MaxQBiQ15311.
PaxDbiQ15311.
PeptideAtlasiQ15311.
PRIDEiQ15311.

Protocols and materials databases

DNASUi10928.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000019317; ENSP00000019317; ENSG00000017797.
ENST00000383432; ENSP00000372924; ENSG00000017797.
GeneIDi10928.
KEGGihsa:10928.
UCSCiuc002kob.4. human.

Organism-specific databases

CTDi10928.
DisGeNETi10928.
GeneCardsiRALBP1.
HGNCiHGNC:9841. RALBP1.
HPAiCAB046010.
HPA046651.
MIMi605801. gene.
neXtProtiNX_Q15311.
OpenTargetsiENSG00000017797.
PharmGKBiPA34199.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4370. Eukaryota.
ENOG410XRJ9. LUCA.
GeneTreeiENSGT00840000129752.
HOGENOMiHOG000007929.
HOVERGENiHBG044496.
InParanoidiQ15311.
KOiK08773.
OMAiYDELMEW.
OrthoDBiEOG091G01CT.
PhylomeDBiQ15311.
TreeFamiTF315411.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000017797-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ15311.

Miscellaneous databases

ChiTaRSiRALBP1. human.
EvolutionaryTraceiQ15311.
GeneWikiiRALBP1.
GenomeRNAii10928.
PROiQ15311.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000017797.
CleanExiHS_RALBP1.
ExpressionAtlasiQ15311. baseline and differential.
GenevisibleiQ15311. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBP1_HUMAN
AccessioniPrimary (citable) accession number: Q15311
Secondary accession number(s): D3DUI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.