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Q15311

- RBP1_HUMAN

UniProt

Q15311 - RBP1_HUMAN

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Protein

RalA-binding protein 1

Gene
RALBP1, RLIP1, RLIP76
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.3 Publications

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB
  2. ATPase activity, coupled to movement of substances Source: UniProtKB
  3. GTPase activator activity Source: UniProtKB
  4. protein binding Source: UniProtKB
  5. Rac GTPase activator activity Source: UniProtKB
  6. Rac GTPase binding Source: UniProtKB
  7. Ral GTPase binding Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. chemotaxis Source: ProtInc
  3. positive regulation of Cdc42 GTPase activity Source: UniProtKB
  4. positive regulation of Rac GTPase activity Source: GOC
  5. regulation of GTPase activity Source: UniProtKB
  6. regulation of small GTPase mediated signal transduction Source: Reactome
  7. signal transduction Source: ProtInc
  8. small GTPase mediated signal transduction Source: UniProtKB
  9. transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
SignaLinkiQ15311.

Protein family/group databases

TCDBi9.A.1.1.1. the non abc multidrug exporter (n-mde) family.

Names & Taxonomyi

Protein namesi
Recommended name:
RalA-binding protein 1
Short name:
RalBP1
Alternative name(s):
76 kDa Ral-interacting protein
Dinitrophenyl S-glutathione ATPase
Short name:
DNP-SG ATPase
Ral-interacting protein 1
Gene namesi
Name:RALBP1
Synonyms:RLIP1, RLIP76
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:9841. RALBP1.

Subcellular locationi

Membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34199.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 655654RalA-binding protein 1PRO_0000056733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication
Modified residuei29 – 291Phosphoserine By similarity
Modified residuei62 – 621Phosphoserine1 Publication
Modified residuei92 – 921Phosphoserine2 Publications
Modified residuei93 – 931Phosphoserine2 Publications
Modified residuei463 – 4631Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ15311.
PaxDbiQ15311.
PeptideAtlasiQ15311.
PRIDEiQ15311.

PTM databases

PhosphoSiteiQ15311.

Expressioni

Tissue specificityi

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.1 Publication

Gene expression databases

ArrayExpressiQ15311.
BgeeiQ15311.
CleanExiHS_RALBP1.
GenevestigatoriQ15311.

Organism-specific databases

HPAiCAB046010.
HPA046651.
HPA054654.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCL20P785562EBI-749285,EBI-3913209
TAX1BP1Q86VP13EBI-749285,EBI-529518

Protein-protein interaction databases

BioGridi116131. 22 interactions.
IntActiQ15311. 8 interactions.
MINTiMINT-140436.
STRINGi9606.ENSP00000019317.

Structurei

Secondary structure

1
655
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi184 – 1874
Helixi194 – 2007
Helixi211 – 22212
Turni223 – 2253
Turni228 – 2325
Helixi237 – 24812
Helixi255 – 2573
Helixi260 – 27314
Helixi280 – 29112
Helixi296 – 30914
Helixi312 – 33423
Helixi339 – 35012
Helixi354 – 36714
Beta strandi384 – 3863
Helixi395 – 41420
Turni417 – 4193
Helixi424 – 44421

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KWHNMR-A393-446[»]
2KWINMR-B393-446[»]
2MBGNMR-A184-446[»]
ProteinModelPortaliQ15311.
SMRiQ15311. Positions 183-446.

Miscellaneous databases

EvolutionaryTraceiQ15311.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 380189Rho-GAPAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni403 – 49997Interacts with RalAAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi164 – 1718Poly-Lys

Sequence similaritiesi

Contains 1 Rho-GAP domain.

Phylogenomic databases

eggNOGiNOG295787.
HOGENOMiHOG000007929.
HOVERGENiHBG044496.
InParanoidiQ15311.
KOiK08773.
OMAiTMMYDGV.
OrthoDBiEOG7V49ZT.
PhylomeDBiQ15311.
TreeFamiTF315411.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q15311-1 [UniParc]FASTAAdd to Basket

« Hide

MTECFLPPTS SPSEHRRVEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP    50
HDILHEPPDV VSDDEKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP 100
SKMKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK 150
SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ IDVPNLKPIF GIPLADAVER 200
TMMYDGIRLP AVFRECIDYV EKYGMKCEGI YRVSGIKSKV DELKAAYDRE 250
ESTNLEDYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGRTTETEKV 300
QEFQRLLKEL PECNYLLISW LIVHMDHVIA KELETKMNIQ NISIVLSPTV 350
QISNRVLYVF FTHVQELFGN VVLKQVMKPL RWSNMATMPT LPETQAGIKE 400
EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE 450
CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME 500
QFLRRQIASE KEEIERLRAE IAEIQSRQQH GRSETEEYSS ESESESEDEE 550
ELQIILEDLQ RQNEELEIKN NHLNQAIHEE REAIIELRVQ LRLLQMQRAK 600
AEQQAQEDEE PEWRGGAVQP PRDGVLEPKA AKEQPKAGKE PAKPSPSRDR 650
KETSI 655
Length:655
Mass (Da):76,063
Last modified:January 23, 2007 - v3
Checksum:iEC6F75329FD8D062
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti617 – 6171A → V.
Corresponds to variant rs35867116 [ dbSNP | Ensembl ].
VAR_049147

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42542 mRNA. Translation: AAB00103.1.
CH471113 Genomic DNA. Translation: EAX01601.1.
CH471113 Genomic DNA. Translation: EAX01602.1.
CH471113 Genomic DNA. Translation: EAX01604.1.
CH471113 Genomic DNA. Translation: EAX01605.1.
BC013126 mRNA. Translation: AAH13126.1.
CCDSiCCDS11845.1.
PIRiF59435.
RefSeqiNP_006779.1. NM_006788.3.
XP_006722358.1. XM_006722295.1.
XP_006722359.1. XM_006722296.1.
UniGeneiHs.528993.

Genome annotation databases

EnsembliENST00000019317; ENSP00000019317; ENSG00000017797.
ENST00000383432; ENSP00000372924; ENSG00000017797.
GeneIDi10928.
KEGGihsa:10928.
UCSCiuc002kob.3. human.

Polymorphism databases

DMDMi34098413.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42542 mRNA. Translation: AAB00103.1 .
CH471113 Genomic DNA. Translation: EAX01601.1 .
CH471113 Genomic DNA. Translation: EAX01602.1 .
CH471113 Genomic DNA. Translation: EAX01604.1 .
CH471113 Genomic DNA. Translation: EAX01605.1 .
BC013126 mRNA. Translation: AAH13126.1 .
CCDSi CCDS11845.1.
PIRi F59435.
RefSeqi NP_006779.1. NM_006788.3.
XP_006722358.1. XM_006722295.1.
XP_006722359.1. XM_006722296.1.
UniGenei Hs.528993.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KWH NMR - A 393-446 [» ]
2KWI NMR - B 393-446 [» ]
2MBG NMR - A 184-446 [» ]
ProteinModelPortali Q15311.
SMRi Q15311. Positions 183-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116131. 22 interactions.
IntActi Q15311. 8 interactions.
MINTi MINT-140436.
STRINGi 9606.ENSP00000019317.

Protein family/group databases

TCDBi 9.A.1.1.1. the non abc multidrug exporter (n-mde) family.

PTM databases

PhosphoSitei Q15311.

Polymorphism databases

DMDMi 34098413.

Proteomic databases

MaxQBi Q15311.
PaxDbi Q15311.
PeptideAtlasi Q15311.
PRIDEi Q15311.

Protocols and materials databases

DNASUi 10928.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000019317 ; ENSP00000019317 ; ENSG00000017797 .
ENST00000383432 ; ENSP00000372924 ; ENSG00000017797 .
GeneIDi 10928.
KEGGi hsa:10928.
UCSCi uc002kob.3. human.

Organism-specific databases

CTDi 10928.
GeneCardsi GC18P009465.
HGNCi HGNC:9841. RALBP1.
HPAi CAB046010.
HPA046651.
HPA054654.
MIMi 605801. gene.
neXtProti NX_Q15311.
PharmGKBi PA34199.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG295787.
HOGENOMi HOG000007929.
HOVERGENi HBG044496.
InParanoidi Q15311.
KOi K08773.
OMAi TMMYDGV.
OrthoDBi EOG7V49ZT.
PhylomeDBi Q15311.
TreeFami TF315411.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.
SignaLinki Q15311.

Miscellaneous databases

ChiTaRSi RALBP1. human.
EvolutionaryTracei Q15311.
GeneWikii RALBP1.
GenomeRNAii 10928.
NextBioi 41515.
PROi Q15311.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q15311.
Bgeei Q15311.
CleanExi HS_RALBP1.
Genevestigatori Q15311.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
InterProi IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view ]
Pfami PF00620. RhoGAP. 1 hit.
[Graphical view ]
SMARTi SM00324. RhoGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS50238. RHOGAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity."
    Jullien-Flores V., Dorseuil O., Romero F., Letourneur F., Saragosti S., Berger R., Tavitian A., Gacon G., Camonis J.H.
    J. Biol. Chem. 270:22473-22477(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RALA.
  2. "Novel function of human RLIP76: ATP-dependent transport of glutathione conjugates and doxorubicin."
    Awasthi S., Cheng J., Singhal S.S., Saini M.K., Pandya U., Pikula S., Bandorowicz-Pikula J., Singh S.V., Zimniak P., Awasthi Y.C.
    Biochemistry 39:9327-9334(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, PROTEIN SEQUENCE OF 2-15; 409-438 AND 472-486.
    Tissue: Bone marrow.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. "Identification and characterization of a novel protein interacting with Ral-binding protein 1, a putative effector protein of Ral."
    Ikeda M., Ishida O., Hinoi T., Kishida S., Kikuchi A.
    J. Biol. Chem. 273:814-821(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH REPS2.
    Tissue: Brain.
  6. "RLIP, an effector of the Ral GTPases, is a platform for Cdk1 to phosphorylate epsin during the switch off of endocytosis in mitosis."
    Rosse C., L'Hoste S., Offner N., Picard A., Camonis J.
    J. Biol. Chem. 278:30597-30604(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CCNB1; CDK1; EPN1; NUMB AND TFAP2A.
  7. "RLIP76 is the major ATP-dependent transporter of glutathione-conjugates and doxorubicin in human erythrocytes."
    Sharma R., Singhal S.S., Cheng J., Yang Y., Sharma A., Zimniak P., Awasthi S., Awasthi Y.C.
    Arch. Biochem. Biophys. 391:171-179(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "AIP1/DAB2IP, a novel member of the Ras-GAP family, transduces TRAF2-induced ASK1-JNK activation."
    Zhang H., Zhang R., Luo Y., D'Alessio A., Pober J.S., Min W.
    J. Biol. Chem. 279:44955-44965(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DAB2IP.
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Platelet.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-93, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92 AND SER-93, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  16. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-463, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. "The RalB-RLIP76 complex reveals a novel mode of ral-effector interaction."
    Fenwick R.B., Campbell L.J., Rajasekar K., Prasannan S., Nietlispach D., Camonis J., Owen D., Mott H.R.
    Structure 18:985-995(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 393-446 IN COMPLEX WITH RALB, INTERACTION WITH RALB.

Entry informationi

Entry nameiRBP1_HUMAN
AccessioniPrimary (citable) accession number: Q15311
Secondary accession number(s): D3DUI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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