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Protein

Reticulocalbin-1

Gene

RCN1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi92 – 103121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi128 – 139122; possibly ancestralPROSITE-ProRule annotationAdd
BLAST
Calcium bindingi179 – 190123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi216 – 227124PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi257 – 268125PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi293 – 304126; possibly ancestralPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Reticulocalbin-1
Gene namesi
Name:RCN1
Synonyms:RCN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:9934. RCN1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: HPA
  • endoplasmic reticulum lumen Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34303.

Polymorphism and mutation databases

BioMutaiRCN1.
DMDMi2493462.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence AnalysisAdd
BLAST
Chaini30 – 331302Reticulocalbin-1PRO_0000004145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi53 – 531N-linked (GlcNAc...)1 Publication
Modified residuei55 – 551Phosphoserine1 Publication
Modified residuei76 – 761Phosphothreonine2 Publications
Modified residuei80 – 801Phosphoserine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ15293.
PeptideAtlasiQ15293.
PRIDEiQ15293.

PTM databases

PhosphoSiteiQ15293.

Expressioni

Gene expression databases

BgeeiQ15293.
CleanExiHS_RCN1.
ExpressionAtlasiQ15293. baseline and differential.
GenevisibleiQ15293. HS.

Organism-specific databases

HPAiHPA038474.
HPA062104.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ATXN1P542533EBI-948278,EBI-930964

Protein-protein interaction databases

BioGridi111887. 71 interactions.
IntActiQ15293. 27 interactions.
MINTiMINT-2862561.
STRINGi9606.ENSP00000054950.

Structurei

3D structure databases

ProteinModelPortaliQ15293.
SMRiQ15293. Positions 47-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 11436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini115 – 15036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini166 – 20136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 23836EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini244 – 27936EF-hand 5PROSITE-ProRule annotationAdd
BLAST
Domaini280 – 31536EF-hand 6PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi328 – 3314Prevents secretion from ER

Sequence similaritiesi

Belongs to the CREC family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG271367.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000230934.
HOVERGENiHBG002834.
InParanoidiQ15293.
OMAiDMFAHEE.
PhylomeDBiQ15293.
TreeFamiTF314849.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR027241. Rcn1.
[Graphical view]
PANTHERiPTHR10827:SF17. PTHR10827:SF17. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 6 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15293-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARGGRGRRL GLALGLLLAL VLAPRVLRAK PTVRKERVVR PDSELGERPP
60 70 80 90 100
EDNQSFQYDH EAFLGKEDSK TFDQLTPDES KERLGKIVDR IDNDGDGFVT
110 120 130 140 150
TEELKTWIKR VQKRYIFDNV AKVWKDYDRD KDDKISWEEY KQATYGYYLG
160 170 180 190 200
NPAEFHDSSD HHTFKKMLPR DERRFKAADL NGDLTATREE FTAFLHPEEF
210 220 230 240 250
EHMKEIVVLE TLEDIDKNGD GFVDQDEYIA DMFSHEENGP EPDWVLSERE
260 270 280 290 300
QFNEFRDLNK DGKLDKDEIR HWILPQDYDH AQAEARHLVY ESDKNKDEKL
310 320 330
TKEEILENWN MFVGSQATNY GEDLTKNHDE L
Length:331
Mass (Da):38,890
Last modified:November 1, 1997 - v1
Checksum:i608AAD536963F789
GO
Isoform 2 (identifier: Q15293-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: MARGGRGRRL...TPDESKERLG → MTGGEGPLPTAKQATCSPPSSRERNPRRRGSRPR

Note: No experimental confirmation available.
Show »
Length:280
Mass (Da):33,026
Checksum:iE62F120400DF5918
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti71 – 711T → N.
Corresponds to variant rs1061145 [ dbSNP | Ensembl ].
VAR_011964
Natural varianti73 – 731D → Y.
Corresponds to variant rs1804281 [ dbSNP | Ensembl ].
VAR_011965
Natural varianti74 – 741Q → E.
Corresponds to variant rs1061143 [ dbSNP | Ensembl ].
VAR_011966
Natural varianti117 – 1171F → L in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035460
Natural varianti188 – 1881R → P.
Corresponds to variant rs1061080 [ dbSNP | Ensembl ].
VAR_011967

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8585MARGG…KERLG → MTGGEGPLPTAKQATCSPPS SRERNPRRRGSRPR in isoform 2. 1 PublicationVSP_055511Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42073 mRNA. Translation: BAA07670.1.
AK293652 mRNA. Translation: BAH11557.1.
AL035078 Genomic DNA. No translation available.
AL049635 Genomic DNA. No translation available.
AL078612 Genomic DNA. No translation available.
AL138970 Genomic DNA. No translation available.
Z95332 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68227.1.
CH471064 Genomic DNA. Translation: EAW68228.1.
BC010120 mRNA. Translation: AAH10120.1.
CCDSiCCDS7876.1. [Q15293-1]
PIRiJC4173.
RefSeqiNP_002892.1. NM_002901.2. [Q15293-1]
UniGeneiHs.97887.

Genome annotation databases

EnsembliENST00000054950; ENSP00000054950; ENSG00000049449. [Q15293-1]
ENST00000532942; ENSP00000436422; ENSG00000049449. [Q15293-2]
GeneIDi5954.
KEGGihsa:5954.
UCSCiuc010reb.2. human. [Q15293-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42073 mRNA. Translation: BAA07670.1.
AK293652 mRNA. Translation: BAH11557.1.
AL035078 Genomic DNA. No translation available.
AL049635 Genomic DNA. No translation available.
AL078612 Genomic DNA. No translation available.
AL138970 Genomic DNA. No translation available.
Z95332 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68227.1.
CH471064 Genomic DNA. Translation: EAW68228.1.
BC010120 mRNA. Translation: AAH10120.1.
CCDSiCCDS7876.1. [Q15293-1]
PIRiJC4173.
RefSeqiNP_002892.1. NM_002901.2. [Q15293-1]
UniGeneiHs.97887.

3D structure databases

ProteinModelPortaliQ15293.
SMRiQ15293. Positions 47-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111887. 71 interactions.
IntActiQ15293. 27 interactions.
MINTiMINT-2862561.
STRINGi9606.ENSP00000054950.

PTM databases

PhosphoSiteiQ15293.

Polymorphism and mutation databases

BioMutaiRCN1.
DMDMi2493462.

Proteomic databases

PaxDbiQ15293.
PeptideAtlasiQ15293.
PRIDEiQ15293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000054950; ENSP00000054950; ENSG00000049449. [Q15293-1]
ENST00000532942; ENSP00000436422; ENSG00000049449. [Q15293-2]
GeneIDi5954.
KEGGihsa:5954.
UCSCiuc010reb.2. human. [Q15293-1]

Organism-specific databases

CTDi5954.
GeneCardsiGC11P031815.
HGNCiHGNC:9934. RCN1.
HPAiHPA038474.
HPA062104.
MIMi602735. gene.
neXtProtiNX_Q15293.
PharmGKBiPA34303.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG271367.
GeneTreeiENSGT00550000074546.
HOGENOMiHOG000230934.
HOVERGENiHBG002834.
InParanoidiQ15293.
OMAiDMFAHEE.
PhylomeDBiQ15293.
TreeFamiTF314849.

Miscellaneous databases

GeneWikiiRCN1.
GenomeRNAii5954.
NextBioi23188.
PROiQ15293.
SOURCEiSearch...

Gene expression databases

BgeeiQ15293.
CleanExiHS_RCN1.
ExpressionAtlasiQ15293. baseline and differential.
GenevisibleiQ15293. HS.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR027241. Rcn1.
[Graphical view]
PANTHERiPTHR10827:SF17. PTHR10827:SF17. 1 hit.
PfamiPF13405. EF-hand_6. 1 hit.
PF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 6 hits.
PS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a human homologue of mouse reticulocalbin reveals conservation of structural domains in the novel endoplasmic reticulum resident Ca(2+)-binding protein with multiple EF-hand motifs."
    Ozawa M.
    J. Biochem. 117:1113-1119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Cerebellum.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Ovary.
  6. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-53.
    Tissue: Liver.
  7. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-76, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; THR-76 AND SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  10. Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-117.

Entry informationi

Entry nameiRCN1_HUMAN
AccessioniPrimary (citable) accession number: Q15293
Secondary accession number(s): B7Z1M1, D3DR00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.