Q15293 (RCN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulocalbin-1

Gene names

Name:	RCN1
Synonyms:	RCN

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Subcellular location	Endoplasmic reticulum lumen.
Miscellaneous	This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium By similarity.
Sequence similarities	Belongs to the CREC family. Contains 6 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
Ligand	Calcium
PTM	Acetylation Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum lumen Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN1	P54253	3	EBI-948278,EBI-930964

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 331	302	Reticulocalbin-1		PRO_0000004145
Regions
	Domain	79 – 114	36	EF-hand 1
	Domain	115 – 150	36	EF-hand 2
	Domain	166 – 201	36	EF-hand 3
	Domain	203 – 238	36	EF-hand 4
	Domain	244 – 279	36	EF-hand 5
	Domain	280 – 315	36	EF-hand 6
	Calcium binding	92 – 103	12	1 Potential
	Calcium binding	128 – 139	12	2; possibly ancestral Potential
	Calcium binding	179 – 190	12	3 Potential
	Calcium binding	216 – 227	12	4 Potential
	Calcium binding	257 – 268	12	5 Potential
	Calcium binding	293 – 304	12	6; possibly ancestral Potential
	Motif	328 – 331	4	Prevents secretion from ER
Amino acid modifications
	Modified residue	86	1	N6-acetyllysine Ref.6
	Glycosylation	53	1	N-linked (GlcNAc...) Ref.5
Natural variations
	Natural variant	71	1	T → N. Corresponds to variant rs1061145 [ dbSNP | Ensembl ].		VAR_011964
	Natural variant	73	1	D → Y. Corresponds to variant rs1804281 [ dbSNP | Ensembl ].		VAR_011965
	Natural variant	74	1	Q → E. Corresponds to variant rs1061143 [ dbSNP | Ensembl ].		VAR_011966
	Natural variant	117	1	F → L in a colorectal cancer sample; somatic mutation. Ref.8		VAR_035460
	Natural variant	188	1	R → P. Corresponds to variant rs1061080 [ dbSNP | Ensembl ].		VAR_011967

Sequences

	Sequence		Length	Mass (Da)
	Q15293 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 608AAD536963F789	FASTA	331	38,890
	10 20 30 40 50 60 MARGGRGRRL GLALGLLLAL VLAPRVLRAK PTVRKERVVR PDSELGERPP EDNQSFQYDH 70 80 90 100 110 120 EAFLGKEDSK TFDQLTPDES KERLGKIVDR IDNDGDGFVT TEELKTWIKR VQKRYIFDNV 130 140 150 160 170 180 AKVWKDYDRD KDDKISWEEY KQATYGYYLG NPAEFHDSSD HHTFKKMLPR DERRFKAADL 190 200 210 220 230 240 NGDLTATREE FTAFLHPEEF EHMKEIVVLE TLEDIDKNGD GFVDQDEYIA DMFSHEENGP 250 260 270 280 290 300 EPDWVLSERE QFNEFRDLNK DGKLDKDEIR HWILPQDYDH AQAEARHLVY ESDKNKDEKL 310 320 330 TKEEILENWN MFVGSQATNY GEDLTKNHDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human homologue of mouse reticulocalbin reveals conservation of structural domains in the novel endoplasmic reticulum resident Ca(2+)-binding protein with multiple EF-hand motifs." Ozawa M. J. Biochem. 117:1113-1119(1995) [PubMed: 8586628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-53, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-86, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] LEU-117.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D42073 mRNA. Translation: BAA07670.1. AL078612 Genomic DNA. No translation available. CH471064 Genomic DNA. Translation: EAW68227.1. CH471064 Genomic DNA. Translation: EAW68228.1. BC010120 mRNA. Translation: AAH10120.1.
IPI	IPI00015842.
PIR	JC4173.
RefSeq	NP_002892.1. NM_002901.2.
UniGene	Hs.97887.
3D structure databases
ProteinModelPortal	Q15293.
SMR	Q15293. Positions 51-311.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15293. 14 interactions.
MINT	MINT-2862561.
STRING	Q15293.
PTM databases
PhosphoSite	Q15293.
Polymorphism databases
DMDM	2493462.
Proteomic databases
PeptideAtlas	Q15293.
PRIDE	Q15293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000054950; ENSP00000054950; ENSG00000049449.
GeneID	5954.
KEGG	hsa:5954.
UCSC	uc001mtj.1. human.
Organism-specific databases
CTD	5954.
GeneCards	GC11P031815.
H-InvDB	HIX0009530. HIX0017761.
HGNC	HGNC:9934. RCN1.
HPA	HPA038474.
MIM	602735. gene.
neXtProt	NX_Q15293.
PharmGKB	PA34303.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG07156.
HOGENOM	HBG388084.
HOVERGEN	HBG002834.
InParanoid	Q15293.
OMA	YIADMFA.
OrthoDB	EOG42RD7R.
PhylomeDB	Q15293.
Gene expression databases
ArrayExpress	Q15293.
Bgee	Q15293.
CleanEx	HS_RCN1.
Genevestigator	Q15293.
GermOnline	ENSG00000049449. Homo sapiens.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca-bd. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 2 hits.
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 4 hits. PS50222. EF_HAND_2. 6 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	23188.
SOURCE	Search...

Entry information

Entry name

RCN1_HUMAN

Accession

Primary (citable) accession number: Q15293
Secondary accession number(s): D3DR00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.