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Protein

Retinoblastoma-binding protein 5

Gene

RBBP5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.By similarity1 Publication

GO - Molecular functioni

  • histone-lysine N-methyltransferase activity Source: Reactome
  • methylated histone binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

  • beta-catenin-TCF complex assembly Source: Reactome
  • cellular response to DNA damage stimulus Source: MGI
  • histone H3-K4 methylation Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to estrogen Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117222-MONOMER.
ReactomeiR-HSA-201722. Formation of the beta-catenin:TCF transactivating complex.
R-HSA-3214841. PKMTs methylate histone lysines.
R-HSA-3769402. Deactivation of the beta-catenin transactivating complex.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
SignaLinkiQ15291.
SIGNORiQ15291.

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoblastoma-binding protein 5
Short name:
RBBP-5
Alternative name(s):
Retinoblastoma-binding protein RBQ-3
Gene namesi
Name:RBBP5
Synonyms:RBQ3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9888. RBBP5.

Subcellular locationi

GO - Cellular componenti

  • histone methyltransferase complex Source: UniProtKB
  • MLL1 complex Source: UniProtKB
  • MLL3/4 complex Source: UniProtKB
  • nucleolus Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • Set1C/COMPASS complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5929.
OpenTargetsiENSG00000117222.
PharmGKBiPA34252.

Chemistry databases

ChEMBLiCHEMBL3137282.

Polymorphism and mutation databases

BioMutaiRBBP5.
DMDMi209572664.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511941 – 538Retinoblastoma-binding protein 5Add BLAST538

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252Phosphothreonine; by CDK11 Publication1
Modified residuei350PhosphoserineCombined sources1
Modified residuei388PhosphoserineCombined sources1
Modified residuei389PhosphoserineCombined sources1
Modified residuei497Phosphoserine; by CDK11 Publication1
Modified residuei525PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ15291.
MaxQBiQ15291.
PaxDbiQ15291.
PeptideAtlasiQ15291.
PRIDEiQ15291.

PTM databases

iPTMnetiQ15291.
PhosphoSitePlusiQ15291.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSG00000117222.
CleanExiHS_RBBP5.
ExpressionAtlasiQ15291. baseline and differential.
GenevisibleiQ15291. HS.

Organism-specific databases

HPAiHPA049042.
HPA058085.

Interactioni

Subunit structurei

Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin. Interacts with WDR5 and ASH2L; the interaction is direct. Interacts with WDR82 and SETD1A. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ASH2LQ9UBL330EBI-592823,EBI-540797
CHD8Q9HCK82EBI-592823,EBI-1169146
CUL4AQ136193EBI-592823,EBI-456106
CXXC1Q9P0U46EBI-592823,EBI-949911
KMT2AQ031646EBI-592823,EBI-591370
PAXIP1Q6ZW4911EBI-592823,EBI-743225
SETD1AO150473EBI-592823,EBI-540779
WDR5P6196412EBI-592823,EBI-540834

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111864. 82 interactors.
DIPiDIP-29224N.
IntActiQ15291. 41 interactors.
MINTiMINT-3031197.
STRINGi9606.ENSP00000264515.

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni349 – 352Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P4FX-ray2.35B371-381[»]
4X8NX-ray2.10B347-356[»]
4X8PX-ray2.20B344-355[»]
5F6KX-ray2.41D/F330-356[»]
5F6LX-ray1.90J330-356[»]
ProteinModelPortaliQ15291.
SMRiQ15291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 63WD 1Add BLAST42
Repeati64 – 103WD 2Add BLAST40
Repeati148 – 188WD 3Add BLAST41
Repeati196 – 235WD 4Add BLAST40
Repeati249 – 291WD 5Add BLAST43
Repeati293 – 331WD 6Add BLAST39

Sequence similaritiesi

Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1273. Eukaryota.
ENOG410XTA2. LUCA.
GeneTreeiENSGT00530000064100.
HOVERGENiHBG054324.
InParanoidiQ15291.
KOiK14961.
OMAiEQGVIEW.
OrthoDBiEOG091G038T.
PhylomeDBiQ15291.
TreeFamiTF313289.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15291-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLELLESFG QNYPEEADGT LDCISMALTC TFNRWGTLLA VGCNDGRIVI
60 70 80 90 100
WDFLTRGIAK IISAHIHPVC SLCWSRDGHK LVSASTDNIV SQWDVLSGDC
110 120 130 140 150
DQRFRFPSPI LKVQYHPRDQ NKVLVCPMKS APVMLTLSDS KHVVLPVDDD
160 170 180 190 200
SDLNVVASFD RRGEYIYTGN AKGKILVLKT DSQDLVASFR VTTGTSNTTA
210 220 230 240 250
IKSIEFARKG SCFLINTADR IIRVYDGREI LTCGRDGEPE PMQKLQDLVN
260 270 280 290 300
RTPWKKCCFS GDGEYIVAGS ARQHALYIWE KSIGNLVKIL HGTRGELLLD
310 320 330 340 350
VAWHPVRPII ASISSGVVSI WAQNQVENWS AFAPDFKELD ENVEYEERES
360 370 380 390 400
EFDIEDEDKS EPEQTGADAA EDEEVDVTSV DPIAAFCSSD EELEDSKALL
410 420 430 440 450
YLPIAPEVED PEENPYGPPP DAVQTSLMDE GASSEKKRQS SADGSQPPKK
460 470 480 490 500
KPKTTNIELQ GVPNDEVHPL LGVKGDGKSK KKQAGRPKGS KGKEKDSPFK
510 520 530
PKLYKGDRGL PLEGSAKGKV QAELSQPLTA GGAISELL
Length:538
Mass (Da):59,153
Last modified:October 14, 2008 - v2
Checksum:i095CCB41613CBED9
GO
Isoform 2 (identifier: Q15291-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     492-529: Missing.

Show »
Length:500
Mass (Da):55,072
Checksum:i45E9CCD4E2448FA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti206F → Y in BAF82826 (PubMed:14702039).Curated1
Sequence conflicti244K → E in CAA59446 (PubMed:7558034).Curated1
Sequence conflicti351E → G in CAA59446 (PubMed:7558034).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035583492 – 529Missing in isoform 2. 2 PublicationsAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85134 mRNA. Translation: CAA59446.1.
AK290137 mRNA. Translation: BAF82826.1.
AL583832, AC093422 Genomic DNA. Translation: CAI15286.1.
AL583832, AC093422 Genomic DNA. Translation: CAI15287.1.
CH471067 Genomic DNA. Translation: EAW91537.1.
CH471067 Genomic DNA. Translation: EAW91538.1.
BC037284 mRNA. Translation: AAH37284.1.
BC053856 mRNA. Translation: AAH53856.1.
BC075059 mRNA. Translation: AAH75059.1.
BC075060 mRNA. Translation: AAH75060.1.
CCDSiCCDS30983.1. [Q15291-1]
CCDS53463.1. [Q15291-2]
PIRiA57624.
RefSeqiNP_001180201.1. NM_001193272.1. [Q15291-2]
NP_001180202.1. NM_001193273.1.
NP_005048.2. NM_005057.3. [Q15291-1]
UniGeneiHs.519230.

Genome annotation databases

EnsembliENST00000264515; ENSP00000264515; ENSG00000117222. [Q15291-1]
ENST00000367164; ENSP00000356132; ENSG00000117222. [Q15291-2]
GeneIDi5929.
KEGGihsa:5929.
UCSCiuc001hbu.3. human. [Q15291-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85134 mRNA. Translation: CAA59446.1.
AK290137 mRNA. Translation: BAF82826.1.
AL583832, AC093422 Genomic DNA. Translation: CAI15286.1.
AL583832, AC093422 Genomic DNA. Translation: CAI15287.1.
CH471067 Genomic DNA. Translation: EAW91537.1.
CH471067 Genomic DNA. Translation: EAW91538.1.
BC037284 mRNA. Translation: AAH37284.1.
BC053856 mRNA. Translation: AAH53856.1.
BC075059 mRNA. Translation: AAH75059.1.
BC075060 mRNA. Translation: AAH75060.1.
CCDSiCCDS30983.1. [Q15291-1]
CCDS53463.1. [Q15291-2]
PIRiA57624.
RefSeqiNP_001180201.1. NM_001193272.1. [Q15291-2]
NP_001180202.1. NM_001193273.1.
NP_005048.2. NM_005057.3. [Q15291-1]
UniGeneiHs.519230.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P4FX-ray2.35B371-381[»]
4X8NX-ray2.10B347-356[»]
4X8PX-ray2.20B344-355[»]
5F6KX-ray2.41D/F330-356[»]
5F6LX-ray1.90J330-356[»]
ProteinModelPortaliQ15291.
SMRiQ15291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111864. 82 interactors.
DIPiDIP-29224N.
IntActiQ15291. 41 interactors.
MINTiMINT-3031197.
STRINGi9606.ENSP00000264515.

Chemistry databases

ChEMBLiCHEMBL3137282.

PTM databases

iPTMnetiQ15291.
PhosphoSitePlusiQ15291.

Polymorphism and mutation databases

BioMutaiRBBP5.
DMDMi209572664.

Proteomic databases

EPDiQ15291.
MaxQBiQ15291.
PaxDbiQ15291.
PeptideAtlasiQ15291.
PRIDEiQ15291.

Protocols and materials databases

DNASUi5929.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264515; ENSP00000264515; ENSG00000117222. [Q15291-1]
ENST00000367164; ENSP00000356132; ENSG00000117222. [Q15291-2]
GeneIDi5929.
KEGGihsa:5929.
UCSCiuc001hbu.3. human. [Q15291-1]

Organism-specific databases

CTDi5929.
DisGeNETi5929.
GeneCardsiRBBP5.
H-InvDBHIX0023636.
HGNCiHGNC:9888. RBBP5.
HPAiHPA049042.
HPA058085.
MIMi600697. gene.
neXtProtiNX_Q15291.
OpenTargetsiENSG00000117222.
PharmGKBiPA34252.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1273. Eukaryota.
ENOG410XTA2. LUCA.
GeneTreeiENSGT00530000064100.
HOVERGENiHBG054324.
InParanoidiQ15291.
KOiK14961.
OMAiEQGVIEW.
OrthoDBiEOG091G038T.
PhylomeDBiQ15291.
TreeFamiTF313289.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000117222-MONOMER.
ReactomeiR-HSA-201722. Formation of the beta-catenin:TCF transactivating complex.
R-HSA-3214841. PKMTs methylate histone lysines.
R-HSA-3769402. Deactivation of the beta-catenin transactivating complex.
R-HSA-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
SignaLinkiQ15291.
SIGNORiQ15291.

Miscellaneous databases

GeneWikiiRBBP5.
GenomeRNAii5929.
PROiQ15291.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117222.
CleanExiHS_RBBP5.
ExpressionAtlasiQ15291. baseline and differential.
GenevisibleiQ15291. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBBP5_HUMAN
AccessioniPrimary (citable) accession number: Q15291
Secondary accession number(s): A8K272, Q7Z6D8, Q8NDZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: October 14, 2008
Last modified: November 30, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.