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Protein

Ras GTPase-activating protein 2

Gene

RASA2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri708 – 74437Btk-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • GTPase activator activity Source: ProtInc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • negative regulation of Ras protein signal transduction Source: GO_Central
  • positive regulation of GTPase activity Source: GOC
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ras GTPase-activating protein 2
Alternative name(s):
GTPase-activating protein 1m
Short name:
GAP1m
Gene namesi
Name:RASA2
Synonyms:GAP1M, RASGAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:9872. RASA2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

Orphaneti648. Noonan syndrome.
PharmGKBiPA34233.

Polymorphism and mutation databases

BioMutaiRASA2.
DMDMi519668674.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 850849Ras GTPase-activating protein 2PRO_0000056638Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ15283.
PaxDbiQ15283.
PRIDEiQ15283.

PTM databases

PhosphoSiteiQ15283.

Expressioni

Gene expression databases

BgeeiQ15283.
CleanExiHS_RASA2.
ExpressionAtlasiQ15283. baseline and differential.
GenevisibleiQ15283. HS.

Organism-specific databases

HPAiHPA035374.
HPA035375.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000286364.

Structurei

3D structure databases

ProteinModelPortaliQ15283.
SMRiQ15283. Positions 30-598, 608-739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 12298C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini158 – 273116C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini356 – 550195Ras-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini604 – 706103PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 2625Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 Btk-type zinc finger.PROSITE-ProRule annotation
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri708 – 74437Btk-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG323905.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ15283.
KOiK08053.
OrthoDBiEOG7J17Z6.
PhylomeDBiQ15283.
TreeFamiTF105302.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH-like_dom.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSiPR00402. TECBTKDOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15283-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAPAAAA ASSEAPAASA TAEPEAGDQD SREVRVLQSL RGKICEAKNL
60 70 80 90 100
LPYLGPHKMR DCFCTINLDQ EEVYRTQVVE KSLSPFFSEE FYFEIPRTFQ
110 120 130 140 150
YLSFYVYDKN VLQRDLRIGK VAIKKEDLCN HSGKETWFSL QPVDSNSEVQ
160 170 180 190 200
GKVHLELKLN ELITENGTVC QQLVVHIKAC HGLPLINGQS CDPYATVSLV
210 220 230 240 250
GPSRNDQKKT KVKKKTSNPQ FNEIFYFEVT RSSSYTRKSQ FQVEEEDIEK
260 270 280 290 300
LEIRIDLWNN GNLVQDVFLG EIKVPVNVLR TDSSHQAWYL LQPRDNGNKS
310 320 330 340 350
SKTDDLGSLR LNICYTEDYV LPSEYYGPLK TLLLKSPDVQ PISASAAYIL
360 370 380 390 400
SEICRDKNDA VLPLVRLLLH HDKLVPFATA VAELDLKDTQ DANTIFRGNS
410 420 430 440 450
LATRCLDEMM KIVGGHYLKV TLKPILDEIC DSSKSCEIDP IKLKEGDNVE
460 470 480 490 500
NNKENLRYYV DKLFNTIVKS SMSCPTVMCD IFYSLRQMAT QRFPNDPHVQ
510 520 530 540 550
YSAVSSFVFL RFFAVAVVSP HTFHLRPHHP DAQTIRTLTL ISKTIQTLGS
560 570 580 590 600
WGSLSKSKSS FKETFMCEFF KMFQEEGYII AVKKFLDEIS STETKESSGT
610 620 630 640 650
SEPVHLKEGE MYKRAQGRTR IGKKNFKKRW FCLTSRELTY HKQPGSKDAI
660 670 680 690 700
YTIPVKNILA VEKLEESSFN KKNMFQVIHT EKPLYVQANN CVEANEWIDV
710 720 730 740 750
LCRVSRCNQN RLSFYHPSVY LNGNWLCCQE TGENTLGCKP CTAGVPADIQ
760 770 780 790 800
IDIDEDRETE RIYSLFTLSL LKLQKMEEAC GTIAVYQGPQ KEPDDYSNFV
810 820 830 840 850
IEDSVTTFKT IQQIKSIIEK LDEPHEKYRK KRSSSAKYGS KENPIVGKAS
Length:850
Mass (Da):96,614
Last modified:June 26, 2013 - v3
Checksum:i1F357940CA106E5E
GO
Isoform 2 (identifier: Q15283-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     646-646: Missing.

Show »
Length:849
Mass (Da):96,527
Checksum:iA4B491DFF5C4CB76
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161T → A in BAA11230 (PubMed:8917095).Curated
Sequence conflicti225 – 2251F → S in BAF84705 (PubMed:14702039).Curated
Sequence conflicti645 – 6451G → EFIER in BAA11621 (PubMed:8812506).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei646 – 6461Missing in isoform 2. 2 PublicationsVSP_046545

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78155 mRNA. Translation: BAA11230.1.
D78156 Genomic DNA. Translation: BAA11231.1.
D82880 mRNA. Translation: BAA11621.1.
D82881 Genomic DNA. Translation: BAA11622.1.
AF115573 mRNA. Translation: AAD09821.1.
AC010184 Genomic DNA. No translation available.
AC092977 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78999.1.
AK292016 mRNA. Translation: BAF84705.1.
CCDSiCCDS3117.1. [Q15283-2]
PIRiJC5047.
RefSeqiNP_001290174.1. NM_001303245.1. [Q15283-1]
NP_001290175.1. NM_001303246.1.
NP_006497.2. NM_006506.3. [Q15283-2]
UniGeneiHs.655941.
Hs.98445.

Genome annotation databases

EnsembliENST00000286364; ENSP00000286364; ENSG00000155903. [Q15283-2]
GeneIDi5922.
KEGGihsa:5922.
UCSCiuc003etz.1. human. [Q15283-1]
uc003eua.1. human.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78155 mRNA. Translation: BAA11230.1.
D78156 Genomic DNA. Translation: BAA11231.1.
D82880 mRNA. Translation: BAA11621.1.
D82881 Genomic DNA. Translation: BAA11622.1.
AF115573 mRNA. Translation: AAD09821.1.
AC010184 Genomic DNA. No translation available.
AC092977 Genomic DNA. No translation available.
CH471052 Genomic DNA. Translation: EAW78999.1.
AK292016 mRNA. Translation: BAF84705.1.
CCDSiCCDS3117.1. [Q15283-2]
PIRiJC5047.
RefSeqiNP_001290174.1. NM_001303245.1. [Q15283-1]
NP_001290175.1. NM_001303246.1.
NP_006497.2. NM_006506.3. [Q15283-2]
UniGeneiHs.655941.
Hs.98445.

3D structure databases

ProteinModelPortaliQ15283.
SMRiQ15283. Positions 30-598, 608-739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000286364.

PTM databases

PhosphoSiteiQ15283.

Polymorphism and mutation databases

BioMutaiRASA2.
DMDMi519668674.

Proteomic databases

MaxQBiQ15283.
PaxDbiQ15283.
PRIDEiQ15283.

Protocols and materials databases

DNASUi5922.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286364; ENSP00000286364; ENSG00000155903. [Q15283-2]
GeneIDi5922.
KEGGihsa:5922.
UCSCiuc003etz.1. human. [Q15283-1]
uc003eua.1. human.

Organism-specific databases

CTDi5922.
GeneCardsiGC03P141205.
HGNCiHGNC:9872. RASA2.
HPAiHPA035374.
HPA035375.
MIMi601589. gene.
neXtProtiNX_Q15283.
Orphaneti648. Noonan syndrome.
PharmGKBiPA34233.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG323905.
GeneTreeiENSGT00760000119092.
HOGENOMiHOG000007183.
HOVERGENiHBG055643.
InParanoidiQ15283.
KOiK08053.
OrthoDBiEOG7J17Z6.
PhylomeDBiQ15283.
TreeFamiTF105302.

Miscellaneous databases

ChiTaRSiRASA2. human.
GenomeRNAii5922.
NextBioi23062.
PROiQ15283.
SOURCEiSearch...

Gene expression databases

BgeeiQ15283.
CleanExiHS_RASA2.
ExpressionAtlasiQ15283. baseline and differential.
GenevisibleiQ15283. HS.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR011993. PH-like_dom.
IPR001849. PH_domain.
IPR023152. RasGAP_CS.
IPR001936. RasGAP_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR001562. Znf_Btk_motif.
[Graphical view]
PfamiPF00779. BTK. 1 hit.
PF00168. C2. 2 hits.
PF00169. PH. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]
PRINTSiPR00402. TECBTKDOMAIN.
SMARTiSM00107. BTK. 1 hit.
SM00239. C2. 2 hits.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
PS51113. ZF_BTK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human rasGTPase-activating protein (human counterpart of GAP1m): sequence of the cDNA, primary structure of the protein, production and chromosomal localization."
    Kobayashi M., Masui T., Kusuda J., Kameoka Y., Hashimoto K., Iwashita S.
    Gene 175:173-177(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
  2. "cDNA cloning and chromosomal mapping of a novel human GAP (GAP1M), a GTPase-activating protein of Ras."
    Li S., Satoh H., Watanabe T., Nakamura S., Hattori S.
    Genomics 35:625-627(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Distinct subcellular localisations of the putative inositol 1,3,4,5-tetrakisphosphate receptors GAP1(IP4BP) and GAP1m result from the GAP1(IP4BP) PH domain directing plasma membrane targeting."
    Lockyer P.J., Bottomley J.R., Reynolds J.S., McNulty T.J., Venkateswarlu K., Potter B.V.L., Dempsey C.E., Cullen P.J.
    Curr. Biol. 7:1007-1010(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Blood.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spleen.
  5. "The DNA sequence, annotation and analysis of human chromosome 3."
    Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
    , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
    Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRASA2_HUMAN
AccessioniPrimary (citable) accession number: Q15283
Secondary accession number(s): A8K7K1
, G3V0F9, O00695, Q15284, Q92594, Q99577, Q9UEQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: June 26, 2013
Last modified: July 22, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.