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Reviewed, UniProtKB/Swiss-Prot Q15173 (2A5B_HUMAN)

Last modified February 9, 2010. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
Alternative name(s):
    PP2A B subunit isoform B'-beta
    PP2A B subunit isoform B56-beta
    PP2A B subunit isoform PR61-beta
    PP2A B subunit isoform R5-beta
Gene names
Name: PPP2R5B
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Subunit structure

PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. Ref.5

Subcellular location

Cytoplasm Ref.4.

Tissue specificity

Highest expression in brain.

Induction

By retinoic acid; in neuroblastoma cell lines.

Post-translational modification

At least isoform Beta-1 is phosphorylated on serine residues. Ref.4

Sequence similarities

Belongs to the phosphatase 2A regulatory subunit B56 family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processsignal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm Ref.4

Traceable author statement. Source: ProtInc

protein phosphatase type 2A complex

Inferred from electronic annotation. Source: InterPro

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

protein phosphatase type 2A regulator activity Ref.4

Traceable author statement. Source: ProtInc

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

CHEK2O960171EBI-1369497,EBI-1180783

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Beta-1 (identifier: Q15173-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta-2 (identifier: Q15173-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: METKLPPASTPTSPSSPGL → MITVNPPLPQDTVNLF

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
PRO_0000071450

Natural variations

Alternative sequence1 – 1919METKL…SSPGL → MITVNPPLPQDTVNLF in isoform Beta-2.
VSP_005109

Experimental info

Sequence conflict57 – 582QE → IF AA sequence Ref.2
Sequence conflict177 – 1782ES → GA AA sequence Ref.2
Sequence conflict1811F → M AA sequence Ref.2
Sequence conflict1841S → M AA sequence Ref.2
Sequence conflict4611W → E AA sequence Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform Beta-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8BEF84F20A77982D

FASTA49757,393
        10         20         30         40         50         60 
METKLPPAST PTSPSSPGLS PVPPPDKVDG FSRRSLRRAR PRRSHSSSQF RYQSNQQELT 

        70         80         90        100        110        120 
PLPLLKDVPA SELHELLSRK LAQCGVMFDF LDCVADLKGK EVKRAALNEL VECVGSTRGV 

       130        140        150        160        170        180 
LIEPVYPDII RMISVNIFRT LPPSENPEFD PEEDEPNLEP SWPHLQLVYE FFLRFLESPD 

       190        200        210        220        230        240 
FQPSVAKRYV DQKFVLMLLE LFDSEDPRER EYLKTILHRV YGKFLGLRAY IRKQCNHIFL 

       250        260        270        280        290        300 
RFIYEFEHFN GVAELLEILG SIINGFALPL KTEHKQFLVR VLIPLHSVKS LSVFHAQLAY 

       310        320        330        340        350        360 
CVVQFLEKDA TLTEHVIRGL LKYWPKTCTQ KEVMFLGEME EILDVIEPSQ FVKIQEPLFK 

       370        380        390        400        410        420 
QVARCVSSPH FQVAERALYF WNNEYILSLI EDNCHTVLPA VFGTLYQVSK EHWNQTIVSL 

       430        440        450        460        470        480 
IYNVLKTFME MNGKLFDELT ASYKLEKQQE QQKAQERQEL WQGLEELRLR RLQGTQGAKE 

       490 
APLQRLTPQV AASGGQS

« Hide

Isoform Beta-2.

Checksum: 5FADF4C0DD9B5507
Show »

FASTA49457,294

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References

« Hide 'large scale' references
[1]"Identification of a new family of protein phosphatase 2A regulatory subunits."
McCright B., Virshup D.M.
J. Biol. Chem. 270:26123-26128(1995) [PubMed: 7592815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-1).
Tissue: Fetal brain.
[2]"The variable subunit associated with protein phosphatase 2A0 defines a novel multimember family of regulatory subunits."
Zolnierowicz S., van Hoof C., Andjelkovic N., Cron P., Stevens I., Merlevede W., Goris J., Hemmings B.A.
Biochem. J. 317:187-194(1996) [PubMed: 8694763] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-2), PARTIAL PROTEIN SEQUENCE.
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA-1).
Tissue: Brain.
[4]"The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm."
McCright B., Rivers A.M., Audlin S., Virshup D.M.
J. Biol. Chem. 271:22081-22089(1996) [PubMed: 8703017] [Abstract]
Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.
[5]"Shugoshin collaborates with protein phosphatase 2A to protect cohesin."
Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y.
Nature 441:46-52(2006) [PubMed: 16541025] [Abstract]
Cited for: INTERACTION WITH SGOL1.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42374 mRNA. Translation: AAC37602.1.
Z69028 mRNA. Translation: CAA93152.1.
BC045619 mRNA. Translation: AAH45619.1.
IPIIPI00014980.
IPI00219690.
PIRI70147.
RefSeqNP_006235.1.
UniGeneHs.75199

3D structure databases

SMRQ15173. Positions 65-437.
ModBaseSearch...

Protein-protein interaction databases

IntActQ15173. 3 interactions.
STRINGQ15173.

PTM databases

PhosphoSiteQ15173.

Proteomic databases

PRIDEQ15173.

Genome annotation databases

EnsemblENST00000164133; ENSP00000164133; ENSG00000068971; Homo sapiens. [Genome view]
GeneID5526.
KEGGhsa:5526.
UCSCuc001oby.1. human.

Organism-specific databases

CTD5526.
GeneCardsGC11P064448.
H-InvDBHIX0009775.
HGNCHGNC:9310. PPP2R5B.
MIM601644. gene.
PharmGKBPA33673.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG17395.
HOGENOMHBG602178.
HOVERGENQ15173.
InParanoidQ15173.
OMAPQVATSG.
OrthoDBEOG970X2R.
PhylomeDBQ15173.

Enzyme and pathway databases

ReactomeREACT_11045. Signaling by Wnt.
REACT_152. Cell Cycle, Mitotic.
REACT_6900. Signaling in Immune system.

Gene expression databases

ArrayExpressQ15173.
BgeeQ15173.
CleanExHS_PPP2R5B.
GenevestigatorQ15173.
GermOnlineENSG00000068971. Homo sapiens.

Family and domain databases

InterProIPR002554. PP2A_B56.
[Graphical view]
PANTHERPTHR10257. B56. 1 hit.
PfamPF01603. B56. 1 hit.
[Graphical view]
PIRSFPIRSF028043. PP2A_B56. 1 hit.
ProtoNetSearch...

Other Resources

NextBio21396.
SOURCESearch...

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Entry information

Entry name2A5B_HUMAN
AccessionPrimary (citable) accession number: Q15173
Secondary accession number(s): Q13853
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents