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Reviewed, UniProtKB/Swiss-Prot Q15154 (PCM1_HUMAN)

Last modified November 25, 2008. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Pericentriolar material 1 protein
      Short name=PCM-1
      Short name=hPCM-1
Gene names
Name: PCM1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length2024 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome.

Subunit structure

Self-associates By similarity. Interacts with BBS4, BBS8, CETN3, HAP1, NDE1 and NDEL1.

Subcellular location

Cytoplasmcytoskeleton. Centrosome. Cytoplasmic granule. Note= Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.

Tissue specificity

Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.

Induction

Expression is reduced in breast and ovarian cancer.

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR.

Involvement in disease

A chromosomal aberration involving PCM1 is found in thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.

A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.

Sequence similarities

Belongs to the PCM1 family.

Sequence caution

The sequence AAH27477.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

The sequence AAH65022.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords

   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityAlternative splicing
Chromosomal rearrangement
Polymorphism
   DiseaseProto-oncogene
   DomainCoiled coil
   PTMPhosphoprotein

Gene Ontology (GO)

   Biological processcentrosome organization

Inferred from mutant phenotype. Source: UniProtKB

cilium biogenesis Ref.8

Inferred from mutant phenotype. Source: UniProtKB

   Cellular componentcentriolar satellite

Inferred from direct assay. Source: UniProtKB

pericentriolar material Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionprotein binding Ref.8 Ref.9

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BBS4Q96RK42EBI-741421,EBI-1805814

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15154-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15154-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1315-1370: RYESASMSSTCEPCKSRNRHSAQTEEPVQAKVFSRKNHEQLEKIIKCNRSTEISSE → K
Isoform 3 (identifier: Q15154-3)

The sequence of this isoform differs from the canonical sequence as follows:
     263-263: R → RENEEEDVRTIDSAVGSGSVAESTSLNIDVQSEASDTTAR
     492-2024: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20242024Pericentriolar material 1 protein
PRO_0000274037

Regions

Region1279 – 1799521Interaction with HAP1
Region1913 – 2024112Interaction with BBS4
Coiled coil218 – 30184 Potential
Coiled coil400 – 42425 Potential
Coiled coil487 – 54357 Potential
Coiled coil651 – 68232 Potential
Coiled coil726 – 76944 Potential
Coiled coil824 – 85835 Potential
Coiled coil1063 – 108927 Potential
Coiled coil1515 – 153925 Potential

Sites

Site1314 – 13152Breakpoint for translocation to form PCM1-JAK2 fusion protein
Site1369 – 13702Breakpoint for translocation to form PCM1-JAK2 fusion protein
Site1470 – 14712Breakpoint for translocation to form PCM1-JAK2 fusion protein
Site1609 – 16102Breakpoint for translocation to form PCM1-RET fusion protein
Site1947 – 19482Breakpoint for translocation to form PCM1-JAK2 fusion protein

Amino acid modifications

Modified residue651Phosphoserine
Modified residue681Phosphoserine
Modified residue691Phosphoserine
Modified residue931Phosphoserine
Modified residue1101Phosphoserine
Modified residue3561Phosphoserine
Modified residue4311Phosphoserine
Modified residue8611Phosphoserine
Modified residue8661Phosphoserine
Modified residue8691Phosphoserine
Modified residue8721Phosphoserine
Modified residue8771Phosphothreonine
Modified residue9601Phosphoserine
Modified residue9911Phosphoserine
Modified residue12311Phosphoserine
Modified residue12571Phosphoserine
Modified residue12601Phosphoserine
Modified residue12621Phosphoserine
Modified residue17301Phosphoserine
Modified residue17651Phosphoserine
Modified residue17681Phosphoserine
Modified residue17761Phosphoserine

Natural variations

Alternative sequence2631R → RENEEEDVRTIDSAVGSGSV AESTSLNIDVQSEASDTTAR in isoform 3.
VSP_022609
Alternative sequence492 – 20241533Missing in isoform 3.
VSP_022610
Alternative sequence1315 – 137056RYESA…EISSE → K in isoform 2.
VSP_022611
Natural variant1591S → N: dbSNP rs412750.
VAR_030164
Natural variant1761A → D: dbSNP rs2285302.
VAR_030165
Natural variant5971V → M: dbSNP rs208753.
VAR_030166
Natural variant6001S → P: dbSNP rs34325017.
VAR_047381
Natural variant6911A → S: dbSNP rs17635381.
VAR_030167
Natural variant8711G → V: dbSNP rs7009117.
VAR_030168
Natural variant12511R → H: dbSNP rs17514547.
VAR_030169
Natural variant13261E → D: dbSNP rs34932823.
VAR_047382
Natural variant15431T → I: dbSNP rs370429.
VAR_030170
Natural variant17011K → N: dbSNP rs36113670.
VAR_047383
Natural variant18651N → D: dbSNP rs35789133.
VAR_047384

Experimental info

Sequence conflict2941R → RG in AAA60120. Ref.1
Sequence conflict311 – 3122EQ → DE in AAA60120. Ref.1
Sequence conflict4051E → K in AAH27477. Ref.3
Sequence conflict4081Q → K in AAH27477 and AAH65022. Ref.3
Sequence conflict4301A → S in AAA60120. Ref.1
Sequence conflict447 – 4482SV → CL in AAA60120. Ref.1
Sequence conflict7601Q → H in AAA60120. Ref.1
Sequence conflict9461G → R in AAA60120. Ref.1
Sequence conflict9521R → T in AAA60120. Ref.1
Sequence conflict10041Missing in AAA60120. Ref.1
Sequence conflict10861Q → R in AAA60120. Ref.1
Sequence conflict11681Q → R in AAA60120. Ref.1
Sequence conflict11691N → I in AAA60120. Ref.1
Sequence conflict11701S → L in AAA60120. Ref.1
Sequence conflict13421V → L in AAA60120. Ref.1
Sequence conflict13821R → Q in AAA60120. Ref.1
Sequence conflict15321T → A in BAC03656. Ref.4
Sequence conflict18491S → G in BAC03656. Ref.4
Sequence conflict1853 – 186412PLERE…SKNDQ → HWNEKPLVKMTK in AAA60120. Ref.1
Sequence conflict18721C → S in AAA60120. Ref.1
Sequence conflict19881E → V in BAC03656. Ref.4
Sequence conflict19981I → M in AAA60120. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 25, 2008. Version 3.
Checksum: 60E74303D3531E42

FASTA2,024228,485
        10         20         30         40         50         60 
MATGGGPFED GMNDQDLPNW SNENVDDRLN NMDWGAQQKK ANRSSEKNKK KFGVESDKRV 

        70         80         90        100        110        120 
TNDISPESSP GVGRRRTKTP HTFPHSRYMS QMSVPEQAEL EKLKQRINFS DLDQRSIGSD 

       130        140        150        160        170        180 
SQGRATAANN KRQLSENRKP FNFLPMQINT NKSKDASTSP PNRETIGSAQ CKELFASALS 

       190        200        210        220        230        240 
NDLLQNCQVS EEDGRGEPAM ESSQIVSRLV QIRDYITKAS SMREDLVEKN ERSANVERLT 

       250        260        270        280        290        300 
HLIDHLKEQE KSYMKFLKKI LARDPQQEPM EEIENLKKQH DLLKRMLQQQ EQLRALQGRQ 

       310        320        330        340        350        360 
AALLALQHKA EQAIAVMDDS VVAETAGSLS GVSITSELNE ELNDLIQRFH NQLRDSQPPA 

       370        380        390        400        410        420 
VPDNRRQAES LSLTREVSQS RKPSASERLP DEKVELFSKM RVLQEKKQKM DKLLGELHTL 

       430        440        450        460        470        480 
RDQHLNNSSA SPQRSVDQRS TSAPSASVGL APVVNGESNS LTSSVPYPTA SLVSQNESEN 

       490        500        510        520        530        540 
EGHLNPSEKL QKLNEVRKRL NELRELVHYY EQTSDMMTDA VNENRKDEET EESEYDSEHE 

       550        560        570        580        590        600 
NSEPVTNIRN PQVASTWNEV NSHSNAQCVS NNRDGRTVNS NCEINNRSAA NIRALNVPPS 

       610        620        630        640        650        660 
LDCRYNREGE QEIHVAQGED DEEEEEEAEE EGVSGASLSS HRSSLVDEHP EDAEFEQKIN 

       670        680        690        700        710        720 
RLMAAKQKLR QLQDLVAMVQ DDDAAQGVIS ASASNLDDFY PAEEDTKQNS NNTRGNANKT 

       730        740        750        760        770        780 
QKDTGVNEKA REKFYEAKLQ QQQRELKQLQ EERKKLIDIQ EKIQALQTAC PDLQLSAASV 

       790        800        810        820        830        840 
GNCPTKKYMP AVTSTPTVNQ HETSTSKSVF EPEDSSIVDN ELWSEMRRHE MLREELRQRR 

       850        860        870        880        890        900 
KQLEALMAEH QRRQGLAETA SPVAVSLRSD GSENLCTPQQ SRTEKTMATW GGSTQCALDE 

       910        920        930        940        950        960 
EGDEDGYLSE GIVRTDEEEE EEQDASSNDN FSVCPSNSVN HNSYNGKETK NRWKNNCPFS 

       970        980        990       1000       1010       1020 
ADENYRPLAK TRQQNISMQR QENLRWVSEL SYVEEKEQWQ EQINQLKKQL DFSVSICQTL 

      1030       1040       1050       1060       1070       1080 
MQDQQTLSCL LQTLLTGPYS VMPSNVASPQ VHFIMHQLNQ CYTQLTWQQN NVQRLKQMLN 

      1090       1100       1110       1120       1130       1140 
ELMRQQNQHP EKPGGKERGS SASHPPSPSL FCPFSFPTQP VNLFNIPGFT NFSSFAPGMN 

      1150       1160       1170       1180       1190       1200 
FSPLFPSNFG DFSQNISTPS EQQQPLAQNS SGKTEYMAFP KPFESSSSIG AEKPRNKKLP 

      1210       1220       1230       1240       1250       1260 
EEEVESSRTP WLYEQEGEVE KPFIKTGFSV SVEKSTSSNR KNQLDTNGRR RQFDEESLES 

      1270       1280       1290       1300       1310       1320 
FSSMPDPVDP TTVTKTFKTR KASAQASLAS KDKTPKSKSK KRNSTQLKSR VKNIRYESAS 

      1330       1340       1350       1360       1370       1380 
MSSTCEPCKS RNRHSAQTEE PVQAKVFSRK NHEQLEKIIK CNRSTEISSE TGSDFSMFEA 

      1390       1400       1410       1420       1430       1440 
LRDTIYSEVA TLISQNESRP HFLIELFHEL QLLNTDYLRQ RALYALQDIV SRHISESHEK 

      1450       1460       1470       1480       1490       1500 
GENVKSVNSG TWIASNSELT PSESLATTDD ETFEKNFERE THKISEQNDA DNASVLSVSS 

      1510       1520       1530       1540       1550       1560 
NFEPFATDDL GNTVIHLDQA LARMREYERM KTEAESNSNM RCTCRIIEDG DGAGAGTTVN 

      1570       1580       1590       1600       1610       1620 
NLEETPVIEN RSSQQPVSEV STIPCPRIDT QQLDRQIKAI MKEVIPFLKE HMDEVCSSQL 

      1630       1640       1650       1660       1670       1680 
LTSVRRMVLT LTQQNDESKE FVKFFHKQLG SILQDSLAKF AGRKLKDCGE DLLVEISEVL 

      1690       1700       1710       1720       1730       1740 
FNELAFFKLM QDLDNNSITV KQRCKRKIEA TGVIQSCAKE AKRILEDHGS PAGEIDDEDK 

      1750       1760       1770       1780       1790       1800 
DKDETETVKQ TQTSEVYDGP KNVRSDISDQ EEDEESEGCP VSINLSKAET QALTNYGSGE 

      1810       1820       1830       1840       1850       1860 
DENEDEEMEE FEEGPVDVQT SLQANTEATE ENEHDEQVLQ RDFKKTAESK NVPLEREATS 

      1870       1880       1890       1900       1910       1920 
KNDQNNCPVK PCYLNILEDE QPLNSAAHKE SPPTVDSTQQ PNPLPLRLPE MEPLVPRVKE 

      1930       1940       1950       1960       1970       1980 
VKSAQETPES SLAGSPDTES PVLVNDYEAE SGNISQKSDE EDFVKVEDLP LKLTIYSEAD 

      1990       2000       2010       2020 
LRKKMVEEEQ KNHLSGEICE MQTEELAGNS ETLKEPETVG AQSI 

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Isoform 2 [UniParc].

Checksum: 89E7E3C696BB65DD
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