Reviewed, UniProtKB/Swiss-Prot Q15147 (PLCB4_HUMAN)

Names and origin

Protein names

Recommended name:
    1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4
    EC=3.1.4.11
Alternative name(s):
    Phosphoinositide phospholipase C-beta-4
    Phospholipase C-beta-4
      Short name=PLC-beta-4

Gene names

Name:

PLCB4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This form has a role in retina signal transduction.
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.
Cofactor	Calcium.
Tissue specificity	Preferentially expressed in the retina.
Sequence similarities	Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain.

Ontologies

Keywords
Biological process	Lipid degradation
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	Calcium
Molecular function	Hydrolase Transducer
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from Experiment. Source: Reactome
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoinositide phospholipase C activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRIN1	Q05586	1	EBI-998637,EBI-998542

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 2 (identifier: Q15147-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q15147-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-153: Missing.
     154-167: LAFMTNTNGKIPVR → MNNNWNVCFFLFCP

Isoform 3 (identifier: Q15147-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1154-1175: AKEMQQMVKLEAEMDRRPATVV → LLKSCHAVSQTQGEGDAADGEIGSRDGPQTSNSSMKLQNAN

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 1175	1174	1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-4		PRO_0000088495
Regions
	Domain	313 – 463	151	PI-PLC X-box
	Domain	565 – 681	117	PI-PLC Y-box
	Domain	688 – 786	99	C2
Sites
	Active site	328	1	By similarity
	Active site	375	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.4
	Modified residue	620	1	Phosphoserine Ref.5
	Modified residue	623	1	Phosphotyrosine Ref.5
	Modified residue	890	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 153	153	Missing in isoform 1.		VSP_004721
	Alternative sequence	154 – 167	14	LAFMT…KIPVR → MNNNWNVCFFLFCP in isoform 1.		VSP_004722
	Alternative sequence	1154 – 1175	22	AKEMQ…PATVV → LLKSCHAVSQTQGEGDAADG EIGSRDGPQTSNSSMKLQNA N in isoform 3.		VSP_037818
	Natural variant	21	1	A → T: dbSNP rs6077510. Ref.3		VAR_056694
	Natural variant	710	1	G → S: dbSNP rs6118603.		VAR_056695
Experimental info
	Sequence conflict	447	1	A → P in AAB02027. Ref.1
	Sequence conflict	757	1	F → L in AAB02027. Ref.1
	Sequence conflict	787	1	L → P in AAB02027. Ref.1
	Sequence conflict	840	1	K → T in AAB02027. Ref.1
	Sequence conflict	902	1	A → P in AAB02027. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified December 5, 2001. Version 3. Checksum: AB2C8EB99EF57357	FASTA	1,175	134,464
	10 20 30 40 50 60 MAKPYEFNWQ KEVPSFLQEG AVFDRYEEES FVFEPNCLFK VDEFGFFLTW RSEGKEGQVL 70 80 90 100 110 120 ECSLINSIRS GAIPKDPKIL AALEAVGKSE NDLEGRIVCV CSGTDLVNIS FTYMVAENPE 130 140 150 160 170 180 VTKQWVEGLR SIIHNFRANN VSPMTCLKKH WMKLAFMTNT NGKIPVRSIT RTFASGKTEK 190 200 210 220 230 240 VIFQALKELG LPSGKNDEIE PTAFSYEKFY ELTQKICPRT DIEDLFKKIN GDKTDYLTVD 250 260 270 280 290 300 QLVSFLNEHQ RDPRLNEILF PFYDAKRAMQ IIEMYEPDED LKKKGLISSD GFCRYLMSDE 310 320 330 340 350 360 NAPVFLDRLE LYQEMDHPLA HYFISSSHNT YLTGRQFGGK SSVEMYRQVL LAGCRCVELD 370 380 390 400 410 420 CWDGKGEDQE PIITHGKAMC TDILFKDVIQ AIKETAFVTS EYPVILSFEN HCSKYQQYKM 430 440 450 460 470 480 SKYCEDLFGD LLLKQALESH PLEPGRALPS PNDLKRKILI KNKRLKPEVE KKQLEALRSM 490 500 510 520 530 540 MEAGESASPA NILEDDNEEE IESADQEEEA HPEFKFGNEL SADDLGHKEA VANSVKKGLV 550 560 570 580 590 600 TVEDEQAWMA SYKYVGATTN IHPYLSTMIN YAQPVKFQGF HVAEERNIHY NMSSFNESVG 610 620 630 640 650 660 LGYLKTHAIE FVNYNKRQMS RIYPKGGRVD SSNYMPQIFW NAGCQMVSLN YQTPDLAMQL 670 680 690 700 710 720 NQGKFEYNGS CGYLLKPDFM RRPDRTFDPF SETPVDGVIA ATCSVQVISG QFLSDKKIGT 730 740 750 760 770 780 YVEVDMYGLP TDTIRKEFRT RMVMNNGLNP VYNEESFVFR KVILPDLAVL RIAVYDDNNK 790 800 810 820 830 840 LIGQRILPLD GLQAGYRHIS LRNEGNKPLS LPTIFCNIVL KTYVPDGFGD IVDALSDPKK 850 860 870 880 890 900 FLSITEKRAD QMRAMGIETS DIADVPSDTS KNDKKGKANT AKANVTPQSS SELRPTTTAA 910 920 930 940 950 960 LASGVEAKKG IELIPQVRIE DLKQMKAYLK HLKKQQKELN SLKKKHAKEH STMQKLHCTQ 970 980 990 1000 1010 1020 VDKIVAQYDK EKSTHEKILE KAMKKKGGSN CLEMKKETEI KIQTLTSDHK SKVKEIVAQH 1030 1040 1050 1060 1070 1080 TKEWSEMINT HSAEEQEIRD LHLSQQCELL KKLLINAHEQ QTQQLKLSHD RESKEMRAHQ 1090 1100 1110 1120 1130 1140 AKISMENSKA ISQDKSIKNK AERERRVREL NSSNTKKFLE ERKRLAMKQS KEMDQLKKVQ 1150 1160 1170 LEHLEFLEKQ NEQAKEMQQM VKLEAEMDRR PATVV
	Isoform 1. Checksum: 4A6A14188161A46C	FASTA	1,022	117,162
	10 20 30 40 50 60 MNNNWNVCFF LFCPSITRTF ASGKTEKVIF QALKELGLPS GKNDEIEPTA FSYEKFYELT 70 80 90 100 110 120 QKICPRTDIE DLFKKINGDK TDYLTVDQLV SFLNEHQRDP RLNEILFPFY DAKRAMQIIE 130 140 150 160 170 180 MYEPDEDLKK KGLISSDGFC RYLMSDENAP VFLDRLELYQ EMDHPLAHYF ISSSHNTYLT 190 200 210 220 230 240 GRQFGGKSSV EMYRQVLLAG CRCVELDCWD GKGEDQEPII THGKAMCTDI LFKDVIQAIK 250 260 270 280 290 300 ETAFVTSEYP VILSFENHCS KYQQYKMSKY CEDLFGDLLL KQALESHPLE PGRALPSPND 310 320 330 340 350 360 LKRKILIKNK RLKPEVEKKQ LEALRSMMEA GESASPANIL EDDNEEEIES ADQEEEAHPE 370 380 390 400 410 420 FKFGNELSAD DLGHKEAVAN SVKKGLVTVE DEQAWMASYK YVGATTNIHP YLSTMINYAQ 430 440 450 460 470 480 PVKFQGFHVA EERNIHYNMS SFNESVGLGY LKTHAIEFVN YNKRQMSRIY PKGGRVDSSN 490 500 510 520 530 540 YMPQIFWNAG CQMVSLNYQT PDLAMQLNQG KFEYNGSCGY LLKPDFMRRP DRTFDPFSET 550 560 570 580 590 600 PVDGVIAATC SVQVISGQFL SDKKIGTYVE VDMYGLPTDT IRKEFRTRMV MNNGLNPVYN 610 620 630 640 650 660 EESFVFRKVI LPDLAVLRIA VYDDNNKLIG QRILPLDGLQ AGYRHISLRN EGNKPLSLPT 670 680 690 700 710 720 IFCNIVLKTY VPDGFGDIVD ALSDPKKFLS ITEKRADQMR AMGIETSDIA DVPSDTSKND 730 740 750 760 770 780 KKGKANTAKA NVTPQSSSEL RPTTTAALAS GVEAKKGIEL IPQVRIEDLK QMKAYLKHLK 790 800 810 820 830 840 KQQKELNSLK KKHAKEHSTM QKLHCTQVDK IVAQYDKEKS THEKILEKAM KKKGGSNCLE 850 860 870 880 890 900 MKKETEIKIQ TLTSDHKSKV KEIVAQHTKE WSEMINTHSA EEQEIRDLHL SQQCELLKKL 910 920 930 940 950 960 LINAHEQQTQ QLKLSHDRES KEMRAHQAKI SMENSKAISQ DKSIKNKAER ERRVRELNSS 970 980 990 1000 1010 1020 NTKKFLEERK RLAMKQSKEM DQLKKVQLEH LEFLEKQNEQ AKEMQQMVKL EAEMDRRPAT VV
	Isoform 3. Checksum: E6A0912E517D182C	FASTA	1,194	136,106
	10 20 30 40 50 60 MAKPYEFNWQ KEVPSFLQEG AVFDRYEEES FVFEPNCLFK VDEFGFFLTW RSEGKEGQVL 70 80 90 100 110 120 ECSLINSIRS GAIPKDPKIL AALEAVGKSE NDLEGRIVCV CSGTDLVNIS FTYMVAENPE 130 140 150 160 170 180 VTKQWVEGLR SIIHNFRANN VSPMTCLKKH WMKLAFMTNT NGKIPVRSIT RTFASGKTEK 190 200 210 220 230 240 VIFQALKELG LPSGKNDEIE PTAFSYEKFY ELTQKICPRT DIEDLFKKIN GDKTDYLTVD 250 260 270 280 290 300 QLVSFLNEHQ RDPRLNEILF PFYDAKRAMQ IIEMYEPDED LKKKGLISSD GFCRYLMSDE 310 320 330 340 350 360 NAPVFLDRLE LYQEMDHPLA HYFISSSHNT YLTGRQFGGK SSVEMYRQVL LAGCRCVELD 370 380 390 400 410 420 CWDGKGEDQE PIITHGKAMC TDILFKDVIQ AIKETAFVTS EYPVILSFEN HCSKYQQYKM 430 440 450 460 470 480 SKYCEDLFGD LLLKQALESH PLEPGRALPS PNDLKRKILI KNKRLKPEVE KKQLEALRSM 490 500 510 520 530 540 MEAGESASPA NILEDDNEEE IESADQEEEA HPEFKFGNEL SADDLGHKEA VANSVKKGLV 550 560 570 580 590 600 TVEDEQAWMA SYKYVGATTN IHPYLSTMIN YAQPVKFQGF HVAEERNIHY NMSSFNESVG 610 620 630 640 650 660 LGYLKTHAIE FVNYNKRQMS RIYPKGGRVD SSNYMPQIFW NAGCQMVSLN YQTPDLAMQL 670 680 690 700 710 720 NQGKFEYNGS CGYLLKPDFM RRPDRTFDPF SETPVDGVIA ATCSVQVISG QFLSDKKIGT 730 740 750 760 770 780 YVEVDMYGLP TDTIRKEFRT RMVMNNGLNP VYNEESFVFR KVILPDLAVL RIAVYDDNNK 790 800 810 820 830 840 LIGQRILPLD GLQAGYRHIS LRNEGNKPLS LPTIFCNIVL KTYVPDGFGD IVDALSDPKK 850 860 870 880 890 900 FLSITEKRAD QMRAMGIETS DIADVPSDTS KNDKKGKANT AKANVTPQSS SELRPTTTAA 910 920 930 940 950 960 LASGVEAKKG IELIPQVRIE DLKQMKAYLK HLKKQQKELN SLKKKHAKEH STMQKLHCTQ 970 980 990 1000 1010 1020 VDKIVAQYDK EKSTHEKILE KAMKKKGGSN CLEMKKETEI KIQTLTSDHK SKVKEIVAQH 1030 1040 1050 1060 1070 1080 TKEWSEMINT HSAEEQEIRD LHLSQQCELL KKLLINAHEQ QTQQLKLSHD RESKEMRAHQ 1090 1100 1110 1120 1130 1140 AKISMENSKA ISQDKSIKNK AERERRVREL NSSNTKKFLE ERKRLAMKQS KEMDQLKKVQ 1150 1160 1170 1180 1190 LEHLEFLEKQ NEQLLKSCHA VSQTQGEGDA ADGEIGSRDG PQTSNSSMKL QNAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." Alvarez R.A., Ghalayini A.J., Xu P., Hardcastle A., Bhattacharya S., Rao P.N., Pettenati M.J., Anderson R.E., Baehr W. Genomics 29:53-61(1995) [PubMed: 8530101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Retina.
[2]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-21. Tissue: Brain.
[4]	Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-11; 79-88; 154-163; 181-195; 220-227; 255-266; 366-377; 435-455; 516-528; 577-586; 606-717; 762-780; 786-797; 822-847; 854-871; 883-908; 910-918; 1023-1039 AND 1139-1149, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-620 AND TYR-623, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-890, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L41349 mRNA. Translation: AAB02027.1. AL031652, AL023805 Genomic DNA. Translation: CAI42213.1. AL023805, AL031652 Genomic DNA. Translation: CAI43087.1. AL031652, AL023805 Genomic DNA. Translation: CAI42218.2. AL023805, AL031652 Genomic DNA. Translation: CAI43092.2. BC117458 mRNA. Translation: AAI17459.1. BC143868 mRNA. Translation: AAI43869.1.
IPI	IPI00014897. IPI00783004. IPI00883983.
RefSeq	NP_000924.2. NP_877949.1.
UniGene	Hs.472101
3D structure databases
SMR	Q15147. Positions 12-821.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15147. 1 interaction.
STRING	Q15147.
PTM databases
PhosphoSite	Q15147.
Proteomic databases
PeptideAtlas	Q5JYT3.
PRIDE	Q15147.
Genome annotation databases
Ensembl	ENST00000278655; ENSP00000278655; ENSG00000101333; Homo sapiens. [Genome view] ENST00000378493; ENSP00000367754; ENSG00000101333; Homo sapiens. [Genome view]
GeneID	5332.
KEGG	hsa:5332.
UCSC	uc002wnf.1. human. uc002wnh.1. human.
Organism-specific databases
CTD	5332.
GeneCards	GC20P009024.
H-InvDB	HIX0015635.
HGNC	HGNC:9059. PLCB4.
MIM	600810. gene.
PharmGKB	PA33387.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15690.
HOVERGEN	Q15147.
OMA	FLTWRSE.
PhylomeDB	Q15147.
Enzyme and pathway databases
BRENDA	3.1.4.11. 247.
Reactome	REACT_14797. Signaling by GPCR. REACT_15295. Opioid Signalling.
Gene expression databases
ArrayExpress	Q15147.
Bgee	Q15147.
Genevestigator	Q15147.
GermOnline	ENSG00000101333. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR018029. C2_membr_targeting. IPR011992. EF-hand-like_dom. IPR015359. Phospholipase_C_EF-hand-like. IPR001192. Phospholipase_C_Pinositol-sp_C. IPR001711. Phospholipase_C_Pinositol-sp_Y. IPR016280. PLC-beta. IPR017946. PLC-like_Pdiesterase_TIM-brl. IPR000909. PLipase_C_PInositol-sp_X_dom. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit. G3DSA:3.20.20.190. PLC-like_Pdiesterase_TIM-brl. 1 hit.
Pfam	PF00168. C2. 1 hit. PF09279. efhand_like. 1 hit. PF00388. PI-PLC-X. 1 hit. PF00387. PI-PLC-Y. 1 hit. [Graphical view]
PIRSF	PIRSF000956. PLC-beta. 1 hit.
PRINTS	PR00390. PHPHLIPASEC.
SMART	SM00239. C2. 1 hit. SM00148. PLCXc. 1 hit. SM00149. PLCYc. 1 hit. [Graphical view]
PROSITE	PS50004. C2. 1 hit. PS50007. PIPLC_X_DOMAIN. 1 hit. PS50008. PIPLC_Y_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	20648.
SOURCE	Search...

Entry information

Entry name

PLCB4_HUMAN

Accession

Primary (citable) accession number: Q15147
Secondary accession number(s): B7ZLK6 , Q17R56, Q5JYS8, Q5JYT0, Q5JYT3, Q5JYT4, Q9BQW5, Q9BQW6, Q9BQW8, Q9UJQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	December 5, 2001
Last modified:	February 9, 2010
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.