Q15131 (CDK10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 10
EC=2.7.11.22

Alternative name(s):
Cell division protein kinase 10
Serine/threonine-protein kinase PISSLRE

Gene names

Name:

CDK10

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of cell proliferation Traceable author statement Ref.1. Source: ProtInc traversing start control point of mitotic cell cycle Traceable author statement Ref.1. Source: ProtInc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Traceable author statement Ref.3. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ETS2	P15036	2	EBI-1646959,EBI-1646991
HSP90AB1	P08238	2	EBI-1646959,EBI-352572
PIN1	Q13526	5	EBI-1646959,EBI-714158

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q15131-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q15131-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q15131-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: Missing.
     306-311: Missing.

Isoform 4 (identifier: Q15131-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-71: Missing.
     329-360: PCEPELMPTFPHHRNKRAAPATSEGQSKRCKP → RLPISGVCEGCREPG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Cyclin-dependent kinase 10		PRO_0000085809
Regions
	Domain	39 – 323	285	Protein kinase
	Nucleotide binding	45 – 53	9	ATP By similarity
Sites
	Active site	163	1	Proton acceptor By similarity
	Binding site	68	1	ATP By similarity
Amino acid modifications
	Modified residue	196	1	Phosphothreonine Ref.10 Ref.11
Natural variations
	Alternative sequence	1 – 71	71	Missing in isoform 2, isoform 3 and isoform 4.		VSP_021642
	Alternative sequence	306 – 311	6	Missing in isoform 3.		VSP_021643
	Alternative sequence	329 – 360	32	PCEPE…KRCKP → RLPISGVCEGCREPG in isoform 4.		VSP_021644
	Natural variant	96	1	P → L. Ref.12		VAR_041983
	Natural variant	168	1	N → S. Ref.12 Corresponds to variant rs56340740 [ dbSNP | Ensembl ].		VAR_041984
	Natural variant	342	1	R → H. Ref.12 Corresponds to variant rs55757604 [ dbSNP | Ensembl ].		VAR_041985
	Natural variant	358	1	C → Y. Ref.12 Corresponds to variant rs56242003 [ dbSNP | Ensembl ].		VAR_041986

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 25B28560961103F4	FASTA	360	41,038
	10 20 30 40 50 60 MAEPDLECEQ IRLKCIRKEG FFTVPPEHRL GRCRSVKEFE KLNRIGEGTY GIVYRARDTQ 70 80 90 100 110 120 TDEIVALKKV RMDKEKDGIP ISSLREITLL LRLRHPNIVE LKEVVVGNHL ESIFLVMGYC 130 140 150 160 170 180 EQDLASLLEN MPTPFSEAQV KCIVLQVLRG LQYLHRNFII HRDLKVSNLL MTDKGCVKTA 190 200 210 220 230 240 DFGLARAYGV PVKPMTPKVV TLWYRAPELL LGTTTQTTSI DMWAVGCILA ELLAHRPLLP 250 260 270 280 290 300 GTSEIHQIDL IVQLLGTPSE NIWPGFSKLP LVGQYSLRKQ PYNNLKHKFP WLSEAGLRLL 310 320 330 340 350 360 HFLFMYDPKK RATAGDCLES SYFKEKPLPC EPELMPTFPH HRNKRAAPAT SEGQSKRCKP
	Isoform 2 [UniParc]. Checksum: 4C1854D5E1858B32	FASTA	289	32,731
	10 20 30 40 50 60 MDKEKDGIPI SSLREITLLL RLRHPNIVEL KEVVVGNHLE SIFLVMGYCE QDLASLLENM 70 80 90 100 110 120 PTPFSEAQVK CIVLQVLRGL QYLHRNFIIH RDLKVSNLLM TDKGCVKTAD FGLARAYGVP 130 140 150 160 170 180 VKPMTPKVVT LWYRAPELLL GTTTQTTSID MWAVGCILAE LLAHRPLLPG TSEIHQIDLI 190 200 210 220 230 240 VQLLGTPSEN IWPGFSKLPL VGQYSLRKQP YNNLKHKFPW LSEAGLRLLH FLFMYDPKKR 250 260 270 280 ATAGDCLESS YFKEKPLPCE PELMPTFPHH RNKRAAPATS EGQSKRCKP
	Isoform 3 [UniParc]. Checksum: C6B5F44BF84F9B88	FASTA	283	31,944
	10 20 30 40 50 60 MDKEKDGIPI SSLREITLLL RLRHPNIVEL KEVVVGNHLE SIFLVMGYCE QDLASLLENM 70 80 90 100 110 120 PTPFSEAQVK CIVLQVLRGL QYLHRNFIIH RDLKVSNLLM TDKGCVKTAD FGLARAYGVP 130 140 150 160 170 180 VKPMTPKVVT LWYRAPELLL GTTTQTTSID MWAVGCILAE LLAHRPLLPG TSEIHQIDLI 190 200 210 220 230 240 VQLLGTPSEN IWPGFSKLPL VGQYSLRKQP YNNLKHKFPW LSEAGLRLLH FLFMATAGDC 250 260 270 280 LESSYFKEKP LPCEPELMPT FPHHRNKRAA PATSEGQSKR CKP
	Isoform 4 [UniParc]. Checksum: 6F30BB76F69F772D	FASTA	272	30,702
	10 20 30 40 50 60 MDKEKDGIPI SSLREITLLL RLRHPNIVEL KEVVVGNHLE SIFLVMGYCE QDLASLLENM 70 80 90 100 110 120 PTPFSEAQVK CIVLQVLRGL QYLHRNFIIH RDLKVSNLLM TDKGCVKTAD FGLARAYGVP 130 140 150 160 170 180 VKPMTPKVVT LWYRAPELLL GTTTQTTSID MWAVGCILAE LLAHRPLLPG TSEIHQIDLI 190 200 210 220 230 240 VQLLGTPSEN IWPGFSKLPL VGQYSLRKQP YNNLKHKFPW LSEAGLRLLH FLFMYDPKKR 250 260 270 ATAGDCLESS YFKEKPLRLP ISGVCEGCRE PG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PISSLRE, a human novel CDC2-related protein kinase." Grana X., Claudio P.P., De Luca A., Sang N., Giordano A. Oncogene 9:2097-2103(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
[2]	Grana X., Claudio P.P., De Luca A., Sang N., Giordano A. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Molecular cloning of PISSLRE, a novel putative member of the cdk family of protein serine/threonine kinases." Brambilla R., Draetta G. Oncogene 9:3037-3041(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"The PISSLRE gene: structure, exon skipping, and exclusion as tumor suppressor in breast cancer." Crawford J., Ianzano L., Savino M., Whitmore S., Cleton-Jansen A.-M., Settasatiani C., D'Apolito M., Seshadri R., Pronk J.C., Auerbach A.D., Verlander P.C., Mathew C.G., Tipping A.J., Doggett N.A., Zelante L., Callen D.F., Savoia A. Genomics 56:90-97(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Colon.
[6]	Bechtel S., Schupp I., Duda A., Wellenreuther R., Mehrle A., Ruschke V., Poustka A., Wiemann S. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[7]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: Colon and Lung.
[10]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-196, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-196, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-96; SER-168; HIS-342 AND TYR-358.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L33264 mRNA. Translation: AAA60092.2. X78342 mRNA. Translation: CAA55137.1. AJ010341 , AJ010342, AJ010343, AJ010344 Genomic DNA. Translation: CAB37619.1. AM392903 mRNA. Translation: CAL37781.1. AK296631 mRNA. Translation: BAH12406.1. AM393177 mRNA. Translation: CAL38055.1. AM393204 mRNA. Translation: CAL38082.1. AC010538 Genomic DNA. No translation available. CH471184 Genomic DNA. Translation: EAW66701.1. CH471184 Genomic DNA. Translation: EAW66703.1. CH471184 Genomic DNA. Translation: EAW66704.1. CH471184 Genomic DNA. Translation: EAW66706.1. CH471184 Genomic DNA. Translation: EAW66707.1. CH471184 Genomic DNA. Translation: EAW66708.1. BC017342 mRNA. Translation: AAH17342.1. BC025301 mRNA. Translation: AAH25301.1.
IPI	IPI00014873. IPI00642595. IPI00788960. IPI01015440.
PIR	S49330.
RefSeq	NP_001092003.2. NM_001098533.2. NP_001153839.1. NM_001160367.1. NP_443713.2. NM_052987.3. NP_443714.3. NM_052988.4.
UniGene	Hs.699177.
3D structure databases
ProteinModelPortal	Q15131.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15131. 4 interactions.
STRING	9606.ENSP00000338673.
PTM databases
PhosphoSite	Q15131.
Polymorphism databases
DMDM	6226784.
Proteomic databases
PaxDb	Q15131.
PRIDE	Q15131.
Protocols and materials databases
DNASU	8558.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000353379; ENSP00000338673; ENSG00000185324. ENST00000505473; ENSP00000424415; ENSG00000185324.
GeneID	8558.
KEGG	hsa:8558.
UCSC	uc002fod.3. human. uc002foe.3. human. uc002fof.3. human.
Organism-specific databases
CTD	8558.
GeneCards	GC16P089753.
HGNC	HGNC:1770. CDK10.
MIM	603464. gene.
neXtProt	NX_Q15131.
PharmGKB	PA26307.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOVERGEN	HBG014652.
InParanoid	Q15131.
KO	K02449.
OMA	MEYCEQD.
OrthoDB	EOG4WSW9V.
PhylomeDB	Q15131.
Enzyme and pathway databases
BRENDA	2.7.11.22. 2681.
Gene expression databases
ArrayExpress	Q15131.
Bgee	Q15131.
CleanEx	HS_CDK10.
Genevestigator	Q15131.
GermOnline	ENSG00000185324. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1795191.
GenomeRNAi	8558.
NextBio	32077.
SOURCE	Search...

Entry information

Entry name

CDK10_HUMAN

Accession

Primary (citable) accession number: Q15131
Secondary accession number(s): A8MXU6 , B7Z420, D3DX82, D3DX83, Q0VGZ7, Q15130, Q6PJC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.