Reviewed, UniProtKB/Swiss-Prot Q15126 (PMVK_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphomevalonate kinase
      Short name=PMKase
    EC=2.7.4.2

Gene names

Name:	PMVK
Synonyms:	PMKI

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate.
Pathway	Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via mevalonic acid pathway; isopentenyl-PP from (R)-mevalonic acid: step 2/3.
Subunit structure	Monomer By similarity.
Subcellular location	Peroxisome.
Tissue specificity	Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta, and lung.
Induction	By sterol.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Peroxisome
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.4 Traceable author statement. Source: ProtInc
Cellular component	peroxisome Ref.4 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphomevalonate kinase activity Ref.4 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CENPH	Q9H3R5	1	EBI-1055562,EBI-1003700
MLF2	Q15773	1	EBI-1055562,EBI-1051875
MPDU1	O75352	1	EBI-1055562,EBI-1046501
PITPNA	Q00169	1	EBI-1055562,EBI-1042490
RGS20	O76081	1	EBI-1055562,EBI-1052678
SSB	P05455	1	EBI-1055562,EBI-358037
TBPL1	P62380	1	EBI-1055562,EBI-716225

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 192	191	Phosphomevalonate kinase		PRO_0000058472
Regions
	Motif	190 – 192	3	Microbody targeting signal Potential
Natural variations
	Natural variant	125	1	V → M: dbSNP rs16836525. Ref.2		VAR_051283

Sequences

	Sequence		Length	Mass (Da)
	Q15126-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D7E720D0DDCCA249	FASTA	192	21,995
	10 20 30 40 50 60 MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ 70 80 90 100 110 120 RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE 130 140 150 160 170 180 AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ 190 LENLIEFIRS RL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence." Chambliss K.L., Slaughter C.A., Schreiner R., Hoffmann G.F., Gibson K.M. J. Biol. Chem. 271:17330-17334(1996) [PubMed: 8663599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-125.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin and Uterus.
[4]	"Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting." Olivier L.M., Chambliss K.L., Gibson K.M., Krisans S.K. J. Lipid Res. 40:672-679(1999) [PubMed: 10191291] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-192.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L77213 mRNA. Translation: AAC37593.1.
BT019976 mRNA. Translation: AAV38779.1.
BC006089 mRNA. Translation: AAH06089.1.
BC007694 mRNA. Translation: AAH07694.1.
AF026069 Genomic DNA. Translation: AAC60791.1.

IPI

IPI00220648.

RefSeq

NP_006547.1.

UniGene

Hs.30954

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CH4	X-ray	1.76	B	1-192	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q15126. 9 interactions.

2-D gel databases

REPRODUCTION-2DPAGE

IPI00220648.

Proteomic databases

PeptideAtlas

Q15126.

PRIDE

Q15126.

Genome annotation databases

Ensembl

ENSG00000163344. Homo sapiens. [Contig view]

GeneID

10654.

KEGG

hsa:10654.

UCSC

uc001ffq.1. human.

Organism-specific databases

GeneCards

GC01M153163.

H-InvDB

HIX0001026.
HIX0076097.

HGNC

HGNC:9141. PMVK.

MIM

607622. gene.

PharmGKB

PA33465.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q15126.

HOVERGEN

Q15126.

OMA

Q15126. QYAQEHG.

Enzyme and pathway databases

BRENDA

2.7.4.2. 247.

Reactome

REACT_602. Metabolism of lipids and lipoproteins.

Gene expression databases

ArrayExpress

Q15126.

Bgee

Q15126.

CleanEx

HS_PMVK.

GermOnline

ENSG00000163344. Homo sapiens.

Family and domain databases

InterPro

IPR005919. Pmev_kin_anim.
[Graphical view]

PANTHER

PTHR13101. Pmev_kin_anim. 1 hit.

Pfam

PF04275. P-mevalo_kinase. 1 hit.
[Graphical view]

PIRSF

PIRSF036639. PMK_anim. 1 hit.

TIGRFAMs

TIGR01223. Pmev_kin_anim. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

40503.

SOURCE

Search...

Entry information

Entry name

PMVK_HUMAN

Accession

Primary (citable) accession number: Q15126
Secondary accession number(s): Q5TZW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)