[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q15120 (PDK3_HUMAN)

Last modified June 16, 2009. Version 91. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial
    EC=2.7.11.2
Alternative name(s):
    Pyruvate dehydrogenase kinase isoform 3

Gene names

Name:

PDK3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Catalytic activity	ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate.
Subcellular location	Mitochondrion matrix.
Tissue specificity	Found almost exclusively in heart and skeletal muscle.
Sequence similarities	Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain.

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Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Mitochondrion
Coding sequence diversity	Polymorphism
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	glucose metabolic process Ref.1 Traceable author statement. Source: ProtInc peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pyruvate dehydrogenase (acetyl-transferring) kinase activity Ref.1 Traceable author statement. Source: ProtInc two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Mitochondrion Potential

Chain

? – 406

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial

PRO_0000023443

Regions

Domain

131 – 362

232

Histidine kinase

Natural variations

Natural variant

219

E → A in a head & Neck squamous cell carcinoma sample; somatic mutation. Ref.3

VAR_042297

Secondary structure

406

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q15120-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: EF2415D3F9D8D61E
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FASTA

406

46,939

        10         20         30         40         50         60 
MRLFRWLLKQ PVPKQIERYS RFSPSPLSIK QFLDFGRDNA CEKTSYMFLR KELPVRLANT 

        70         80         90        100        110        120 
MREVNLLPDN LLNRPSVGLV QSWYMQSFLE LLEYENKSPE DPQVLDNFLQ VLIKVRNRHN 

       130        140        150        160        170        180 
DVVPTMAQGV IEYKEKFGFD PFISTNIQYF LDRFYTNRIS FRMLINQHTL LFGGDTNPVH 

       190        200        210        220        230        240 
PKHIGSIDPT CNVADVVKDA YETAKMLCEQ YYLVAPELEV EEFNAKAPDK PIQVVYVPSH 

       250        260        270        280        290        300 
LFHMLFELFK NSMRATVELY EDRKEGYPAV KTLVTLGKED LSIKISDLGG GVPLRKIDRL 

       310        320        330        340        350        360 
FNYMYSTAPR PSLEPTRAAP LAGFGYGLPI SRLYARYFQG DLKLYSMEGV GTDAVIYLKA 

       370        380        390        400 
LSSESFERLP VFNKSAWRHY KTTPEADDWS NPSSEPRDAS KYKAKQ

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Diversity of the pyruvate dehydrogenase kinase gene family in humans." Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M. J. Biol. Chem. 270:28989-28994(1995) [PubMed: 7499431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[3]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-219.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

L42452 mRNA. Translation: AAC42011.1.
BC015948 mRNA. Translation: AAH15948.1.

IPI

IPI00014849.

PIR

I70160.

RefSeq

NP_001135858.1.
NP_005382.1.

UniGene

Hs.658190

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Y8N	X-ray	2.60	A	9-406	[»]
1Y8O	X-ray	2.48	A	9-406	[»]
1Y8P	X-ray	2.63	A	9-406	[»]
2PNR	X-ray	2.50	A/B/E/F	9-406	[»]
2Q8I	X-ray	2.60	A	9-406	[»]

ModBase

Search...

Proteomic databases

PRIDE

Q15120.

Genome annotation databases

Ensembl

ENSG00000067992. Homo sapiens. [Contig view]

GeneID

5165.

KEGG

hsa:5165.

Organism-specific databases

GeneCards

GC0XP024393.

H-InvDB

HIX0016707.

HGNC

HGNC:8811. PDK3.

MIM

602526. gene.

PharmGKB

PA33156.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q15120.

HOVERGEN

Q15120.

OMA

Q15120. APNKPIQ.

Enzyme and pathway databases

BRENDA

2.7.11.2. 247.

Reactome

REACT_1505. Integration of energy metabolism.

Gene expression databases

ArrayExpress

Q15120.

Bgee

Q15120.

CleanEx

HS_PDK3.

GermOnline

ENSG00000067992. Homo sapiens.

Family and domain databases

InterPro

IPR003594. ATP_bd_ATPase.
IPR018955. BCDHK/PDK_mit.
IPR005467. Sig_transdc_His_kinase_core.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.

Pfam

PF10436. BCDHK_Adom3. 1 hit.
PF02518. HATPase_c. 1 hit.
[Graphical view]

SMART

SM00387. HATPase_c. 1 hit.
[Graphical view]

PROSITE

PS50109. HIS_KIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

19982.

SOURCE

Search...

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Entry information

Entry name

PDK3_HUMAN

Accession

Primary (citable) accession number: Q15120

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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