Reviewed, UniProtKB/Swiss-Prot Q15072 (OZF_HUMAN)

Names and origin

Protein names

Recommended name:
    Zinc finger protein OZF
Alternative name(s):
    Only zinc finger protein
    Zinc finger protein 146

Gene names

Name:	ZNF146
Synonyms:	OZF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Binds DNA. Interacts with SUMO conjugating enzyme UBC9/UBE2I. Interacts with the telomeric protein TERF2IP. Ref.8 Ref.9
Subcellular location	Nucleus. Ref.5
Tissue specificity	Liver, skeletal and heart muscle, mammary cells. Very low levels in brain, lung, placenta and kidney. Ref.5 Ref.1
Post-translational modification	Sumoylated. Ref.9
Miscellaneous	Strongly overexpressed in many pancreas and colorectal cancers. Increased gene copy numbers are detected in 3 of 12 tumor cell lines and 2 of 12 primary pancreatic carcinomas. Overexpresseed in 80% of colorectal cancers.
Sequence similarities	Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 10 C2H2-type zinc fingers.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA
Molecular function	DNA binding Ref.5 Traceable author statement. Source: ProtInc heparin binding Ref.5 Traceable author statement. Source: ProtInc zinc ion binding Ref.5 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Zinc finger protein OZF		PRO_0000047276
Regions
	Zinc finger	16 – 38	23	C2H2-type 1
	Zinc finger	44 – 66	23	C2H2-type 2
	Zinc finger	72 – 94	23	C2H2-type 3
	Zinc finger	100 – 122	23	C2H2-type 4
	Zinc finger	128 – 150	23	C2H2-type 5
	Zinc finger	156 – 178	23	C2H2-type 6
	Zinc finger	184 – 206	23	C2H2-type 7
	Zinc finger	212 – 234	23	C2H2-type 8
	Zinc finger	240 – 262	23	C2H2-type 9
	Zinc finger	268 – 290	23	C2H2-type 10
	Region	212 – 292	81	Interaction with TERF2IP
Amino acid modifications
	Cross-link	157		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	169		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Natural variations
	Natural variant	8	1	R → K: dbSNP rs2070132. Ref.1 Ref.2 Ref.4		VAR_023746
Experimental info
	Mutagenesis	157	1	K → R: Induces a decrease in sumoylation. Induces a strong decrease but does not abolishes sumoylation; when associated with R-169. Ref.9
	Mutagenesis	169	1	K → R: Induces a decrease in sumoylation. Induces a strong decrease but does not abolishes sumoylation; when associated with R-157. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q15072-1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: D5062375D261B971	FASTA	292	33,308
	10 20 30 40 50 60 MSHLSQQRIY SGENPFACKV CGKVFSHKSN LTEHEHFHTR EKPFECNECG KAFSQKQYVI 70 80 90 100 110 120 KHQNTHTGEK LFECNECGKS FSQKENLLTH QKIHTGEKPF ECKDCGKAFI QKSNLIRHQR 130 140 150 160 170 180 THTGEKPFVC KECGKTFSGK SNLTEHEKIH IGEKPFKCSE CGTAFGQKKY LIKHQNIHTG 190 200 210 220 230 240 EKPYECNECG KAFSQRTSLI VHVRIHSGDK PYECNVCGKA FSQSSSLTVH VRSHTGEKPY 250 260 270 280 290 GCNECGKAFS QFSTLALHLR IHTGKKPYQC SECGKAFSQK SHHIRHQKIH TH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The OZF gene encodes a protein consisting essentially of zinc finger motifs." le Chalony C., Prosperi M.-T., Haluza R., Apiou F., Dutrillaux B., Goubin G. J. Mol. Biol. 236:399-404(1994) [PubMed: 8107129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, VARIANT LYS-8. Tissue: Mammary gland.
[2]	"Genomic organization and promoter identification of ZNF146, a gene encoding a protein consisting solely of zinc finger domains." Pibouin L., Villaudy J., Prosperi M.-T., Goubin G. Cytogenet. Cell Genet. 92:80-84(2001) [PubMed: 11306801] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT LYS-8.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LYS-8.
[5]	"Identification, nuclear localization, and binding activities of OZF, a human protein solely composed of zinc-finger motifs." Ferbus D., le Chalony C., Prosperi M.-T., Muleris M., Vincent-Salomon A., Goubin G. Eur. J. Biochem. 236:991-995(1996) [PubMed: 8665923] [Abstract] Cited for: DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]	"Amplification and over-expression of OZF, a gene encoding a zinc finger protein, in human pancreatic carcinomas." Ferbus D., Flechon A., Muleris M., Li Y., Hanash S., Terris B., Hammel P., Pibouin L., Dutrillaux B., Goubin G. Int. J. Cancer 80:369-372(1999) [PubMed: 9935176] [Abstract] Cited for: OVEREXPRESSION IN PANCREATIC CANCER.
[7]	"The zinc finger protein OZF (ZNF146) is overexpressed in colorectal cancer." Ferbus D., Bovin C., Validire P., Goubin G. J. Pathol. 200:177-182(2003) [PubMed: 12754738] [Abstract] Cited for: OVEREXPRESSION IN COLORECTAL CANCER.
[8]	"Zinc finger protein overexpressed in colon carcinoma interacts with the telomeric protein hRap1." Antoine K., Ferbus D., Kolahgar G., Prosperi M.-T., Goubin G. J. Cell. Biochem. 95:763-768(2005) [PubMed: 15838871] [Abstract] Cited for: INTERACTION WITH TERF2IP.
[9]	"A Kruppel zinc finger of ZNF 146 interacts with the SUMO-1 conjugating enzyme UBC9 and is sumoylated in vivo." Antoine K., Prosperi M.-T., Ferbus D., Boule C., Goubin G. Mol. Cell. Biochem. 271:215-223(2005) [PubMed: 15881673] [Abstract] Cited for: INTERACTION WITH UBE2I, SUMOYLATION AT LYS-157 AND LYS-169, MUTAGENESIS OF LYS-157 AND LYS-169.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70394 mRNA. Translation: CAA49844.1. AJ011806 Genomic DNA. Translation: CAB41967.1. AC012617 Genomic DNA. No translation available. BC110500 mRNA. Translation: AAI10501.1. BC110501 mRNA. Translation: AAI10502.1.
IPI	IPI00014305.
PIR	S43826.
RefSeq	NP_001093108.1. NP_001093109.1. NP_009076.2.
UniGene	Hs.643436
3D structure databases
HSSP	HSSP built from PDB template 1SP2 based on UniProtKB P08047.
ModBase	Search...
PTM databases
PhosphoSite	Q15072.
Genome annotation databases
Ensembl	ENSG00000167635. Homo sapiens. [Contig view]
GeneID	7705.
KEGG	hsa:7705.
Organism-specific databases
GeneCards	GC19P041411.
HGNC	HGNC:12931. ZNF146.
HPA	HPA003358.
MIM	601505. gene.
PharmGKB	PA37518.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q15072.
HOVERGEN	Q15072.
OMA	Q15072. GSPFACK.
Gene expression databases
ArrayExpress	Q15072.
Bgee	Q15072.
CleanEx	HS_ZNF146.
GermOnline	ENSG00000167635. Homo sapiens.
Family and domain databases
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 10 hits.
Pfam	PF00096. zf-C2H2. 10 hits. [Graphical view]
ProDom	PD000003. Znf_C2H2. 10 hits. [Graphical view] [Entries sharing at least one domain]
SMART	SM00355. ZnF_C2H2. 10 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 10 hits. PS50157. ZINC_FINGER_C2H2_2. 10 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	29868.
SOURCE	Search...

Entry information

Entry name

OZF_HUMAN

Accession

Primary (citable) accession number: Q15072
Secondary accession number(s): Q2TB94

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 6, 2007
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)