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Reviewed, UniProtKB/Swiss-Prot Q15063 (POSTN_HUMAN)

Last modified November 25, 2008. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Periostin
      Short name=PN
Alternative name(s):
    Osteoblast-specific factor 2
      Short name=OSF-2
Gene names
Name: POSTN
Synonyms: OSF2
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length836 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.

Subcellular location

Secreted › extracellular space › extracellular matrix.

Tissue specificity

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.

Post-translational modification

Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Sequence similarities

Contains 1 EMI domain.

Contains 4 FAS1 domains.

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Ontologies

Keywords

   Biological processCell adhesion
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
   LigandHeparin-binding
   PTMGamma-carboxyglutamic acid
Glycoprotein

Gene Ontology (GO)

   Biological processcell adhesion Ref.2

Inferred from direct assay. Source: UniProtKB

skeletal system development Ref.1

Traceable author statement. Source: ProtInc

   Cellular componentproteinaceous extracellular matrix

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular functionheparin binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15063-1)

Also known as: OSF-2OS;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15063-2)

Also known as: OSF-2p1;

The sequence of this isoform differs from the canonical sequence as follows:
     670-727: TTKIITKVVEPKIKVIEGSLQPIIKTEGPTLTKVKIEGEPEFRLIKEGETITEVIHGE → K
Isoform 3 (identifier: Q15063-3)

The sequence of this isoform differs from the canonical sequence as follows:
     670-697: TTKIITKVVEPKIKVIEGSLQPIIKTEG → R
     783-810: Missing.
Isoform 4 (identifier: Q15063-4)

The sequence of this isoform differs from the canonical sequence as follows:
     670-727: TTKIITKVVEPKIKVIEGSLQPIIKTEGPTLTKVKIEGEPEFRLIKEGETITEVIHGE → K
     783-810: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 836815Periostin
PRO_0000008789

Regions

Domain40 – 9455EMI
Domain97 – 230134FAS1 1
Domain234 – 365132FAS1 2
Domain368 – 492125FAS1 3
Domain496 – 628133FAS1 4

Amino acid modifications

Modified residue12414-carboxyglutamate Potential
Modified residue12514-carboxyglutamate Potential
Modified residue12714-carboxyglutamate Potential
Modified residue14014-carboxyglutamate Potential
Modified residue15414-carboxyglutamate Potential
Modified residue16014-carboxyglutamate Potential
Modified residue24214-carboxyglutamate Potential
Modified residue24414-carboxyglutamate Potential
Modified residue26114-carboxyglutamate Potential
Modified residue27714-carboxyglutamate Potential
Modified residue28014-carboxyglutamate Potential
Modified residue28814-carboxyglutamate Potential
Modified residue29814-carboxyglutamate Potential
Modified residue31314-carboxyglutamate Potential
Modified residue32214-carboxyglutamate Potential
Modified residue32514-carboxyglutamate Potential
Modified residue49614-carboxyglutamate Potential
Modified residue50114-carboxyglutamate Potential
Modified residue51714-carboxyglutamate Potential
Modified residue52314-carboxyglutamate Potential
Modified residue54714-carboxyglutamate Potential
Modified residue54814-carboxyglutamate Potential
Modified residue55014-carboxyglutamate Potential
Modified residue57814-carboxyglutamate Potential
Glycosylation5991N-linked (GlcNAc...)
Disulfide bond44 ↔ 80 By similarity
Disulfide bond60 ↔ 69 By similarity
Disulfide bond79 ↔ 92 By similarity

Natural variations

Alternative sequence670 – 72758TTKII…VIHGE → K in isoform 2 and isoform 4.
VSP_050005
Alternative sequence670 – 69728TTKII…IKTEG → R in isoform 3.
VSP_050669
Alternative sequence783 – 81028Missing in isoform 3 and isoform 4.
VSP_050670

Experimental info

Sequence conflict2901I → F in BAA02836 and BAA02837. Ref.1
Sequence conflict2901I → F in AAY15840. Ref.3
Sequence conflict4211D → V in BAA02836 and BAA02837. Ref.1
Sequence conflict4211D → V in AAY15840. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (OSF-2OS) [UniParc].

Last modified March 7, 2006. Version 2.
Checksum: 55E7B82D094824FD

FASTA83693,314
        10         20         30         40         50         60 
MIPFLPMFSL LLLLIVNPIN ANNHYDKILA HSRIRGRDQG PNVCALQQIL GTKKKYFSTC 

        70         80         90        100        110        120 
KNWYKKSICG QKTTVLYECC PGYMRMEGMK GCPAVLPIDH VYGTLGIVGA TTTQRYSDAS 

       130        140        150        160        170        180 
KLREEIEGKG SFTYFAPSNE AWDNLDSDIR RGLESNVNVE LLNALHSHMI NKRMLTKDLK 

       190        200        210        220        230        240 
NGMIIPSMYN NLGLFINHYP NGVVTVNCAR IIHGNQIATN GVVHVIDRVL TQIGTSIQDF 

       250        260        270        280        290        300 
IEAEDDLSSF RAAAITSDIL EALGRDGHFT LFAPTNEAFE KLPRGVLERI MGDKVASEAL 

       310        320        330        340        350        360 
MKYHILNTLQ CSESIMGGAV FETLEGNTIE IGCDGDSITV NGIKMVNKKD IVTNNGVIHL 

       370        380        390        400        410        420 
IDQVLIPDSA KQVIELAGKQ QTTFTDLVAQ LGLASALRPD GEYTLLAPVN NAFSDDTLSM 

       430        440        450        460        470        480 
DQRLLKLILQ NHILKVKVGL NELYNGQILE TIGGKQLRVF VYRTAVCIEN SCMEKGSKQG 

       490        500        510        520        530        540 
RNGAIHIFRE IIKPAEKSLH EKLKQDKRFS TFLSLLEAAD LKELLTQPGD WTLFVPTNDA 

       550        560        570        580        590        600 
FKGMTSEEKE ILIRDKNALQ NIILYHLTPG VFIGKGFEPG VTNILKTTQG SKIFLKEVND 

       610        620        630        640        650        660 
TLLVNELKSK ESDIMTTNGV IHVVDKLLYP ADTPVGNDQL LEILNKLIKY IQIKFVRGST 

       670        680        690        700        710        720 
FKEIPVTVYT TKIITKVVEP KIKVIEGSLQ PIIKTEGPTL TKVKIEGEPE FRLIKEGETI 

       730        740        750        760        770        780 
TEVIHGEPII KKYTKIIDGV PVEITEKETR EERIITGPEI KYTRISTGGG ETEETLKKLL 

       790        800        810        820        830 
QEEVTKVTKF IEGGDGHLFE DEEIKRLLQG DTPVRKLQAN KKVQGSRRRL REGRSQ

« Hide

Isoform 2 (OSF-2p1) [UniParc].

Checksum: C6936F32A42B8366
Show »

77987,021
Isoform 3 [UniParc].

Checksum: D082BC8F86ABAC37
Show »

78187,254
Isoform 4 [UniParc].

Checksum: 7241170E4CDC18D3
Show »

75183,850

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References

« Hide 'large scale' references
[1]"Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin I."
Takeshita S., Kikuno R., Tezuka K., Amann E.
Biochem. J. 294:271-278(1993) [PubMed: 8363580] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Osteosarcoma and Placenta.
[2]"Periostin secreted by epithelial ovarian carcinoma is a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins and promotes cell motility."
Gillan L., Matei D., Fishman D.A., Gerbin C.S., Karlan B.Y., Chang D.D.
Cancer Res. 62:5358-5364(2002) [PubMed: 12235007] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"Identification and characterization of a novel periodontal ligament-specific periostin isoform."
Yamada S., Maeda K., Matsubara K., Murakami S.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
Tissue: Periodontal ligament.
[4]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed: 15057823] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]"Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas."
Sasaki H., Dai M., Auclair D., Fukai I., Kiriyama M., Yamakawa Y., Fujii Y., Chen L.B.
Cancer 92:843-848(2001) [PubMed: 11550156] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]Erratum
Sasaki H., Dai M., Auclair D., Fukai I., Kiriyama M., Yamakawa Y., Fujii Y., Chen L.B.
Cancer 95:2580-2580(2002)
[9]"Acquired expression of periostin by human breast cancers promotes tumor angiogenesis through up-regulation of vascular endothelial growth factor receptor 2 expression."
Shao R., Bao S., Bai X., Blanchette C., Anderson R.M., Dang T., Gishizky M.L., Marks J.R., Wang X.-F.
Mol. Cell. Biol. 24:3992-4003(2004) [PubMed: 15082792] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J., Smith R.D.
J. Proteome Res. 4:2070-2080(2005) [PubMed: 16335952] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-599, MASS SPECTROMETRY.
Tissue: Plasma.
[11]"Periostin, a member of a novel family of vitamin K-dependent proteins, is expressed by mesenchymal stromal cells."
Coutu D.L., Wu J.H., Monette A., Rivard G.-E., Blostein M.D., Galipeau J.
J. Biol. Chem. 283:17991-18001(2008) [PubMed: 18450759] [Abstract]
Cited for: FUNCTION, GAMMA-CARBOXYGLUTAMATION.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D13665 mRNA. Translation: BAA02836.1.
D13666 mRNA. Translation: BAA02837.1.
AY140646 mRNA. Translation: AAN17733.1.
AY918092 mRNA. Translation: AAY15840.1.
AL138679, AL646087 Genomic DNA. Translation: CAH70107.1.
AL646087, AL138679 Genomic DNA. Translation: CAH73568.1.
AL138679, AL646087 Genomic DNA. Translation: CAH70104.1.
AL646087, AL138679 Genomic DNA. Translation: CAH73569.1.
CH471075 Genomic DNA. Translation: EAX08590.1.
BC106709 mRNA. Translation: AAI06710.1.
BC106710 mRNA. Translation: AAI06711.1.
PIRS36110.
S36111.
RefSeqNP_006466.1.
UniGeneHs.136348