Q15061 (WDR43_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WD repeat-containing protein 43

Gene names

Name:	WDR43
Synonyms:	KIAA0007

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Nucleus › nucleolus Ref.2.
Sequence similarities	Belongs to the UTP5 family. Contains 6 WD repeats.
Sequence caution	The sequence BAA05499.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA05499.1 differs from that shown. Reason: Frameshift at positions 614 and 617.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 677	677	WD repeat-containing protein 43		PRO_0000051392
Regions
	Repeat	11 – 51	41	WD 1
	Repeat	57 – 119	63	WD 2
	Repeat	124 – 163	40	WD 3
	Repeat	166 – 205	40	WD 4
	Repeat	207 – 259	53	WD 5
	Repeat	267 – 309	43	WD 6
Amino acid modifications
	Modified residue	77	1	Phosphoserine Ref.3 Ref.7
	Modified residue	394	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	431	1	Phosphoserine Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
	Modified residue	437	1	Phosphoserine Ref.7
	Modified residue	603	1	Phosphoserine Ref.3
	Modified residue	604	1	Phosphoserine Ref.3
	Modified residue	608	1	Phosphoserine Ref.3
	Modified residue	658	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q15061 [UniParc]. Last modified July 24, 2007. Version 3. Checksum: 4EEAD92C30118ACD	FASTA	677	74,891
	10 20 30 40 50 60 MAAGGGGSCD PLAPAGVPCA FSPHSQAYFA LASTDGHLRV WETANNRLHQ EYVPSAHLSG 70 80 90 100 110 120 TCTCLAWAPA RLQAKESPQR KKRKSEAVGM SNQTDLLALG TAVGSILLYS TVKGELHSKL 130 140 150 160 170 180 ISGGHDNRVN CIQWHQDSGC LYSCSDDKHI VEWNVQTCKV KCKWKGDNSS VSSLCISPDG 190 200 210 220 230 240 KMLLSAGRTI KLWVLETKEV YRHFTGHATP VSSLMFTTIR PPNESQPFDG ITGLYFLSGA 250 260 270 280 290 300 VHDRLLNVWQ VRSENKEKSA VMSFTVTDEP VYIDLTLSEN KEEPVKLAVV CRDGQVHLFE 310 320 330 340 350 360 HILNGYCKKP LTSNCTIQIA TPGKGKKSTP KPIPILAAGF CSDKMSLLLV YGSWFQPTIE 370 380 390 400 410 420 RVALNSREPH MCLVRDISNC WAPKVETAIT KVRTPVMNSE AKVLVPGIPG HHAAIKPAPP 430 440 450 460 470 480 QTEQVESKRK SGGNEVSIEE RLGAMDIDTH KKGKEDLQTN SFPVLLTQGL ESNDFEMLNK 490 500 510 520 530 540 VLQTRNVNLI KKTVLRMPLH TIIPLLQELT KRLQGHPNSA VLMVQWLKCV LTVHASYLST 550 560 570 580 590 600 LPDLVPQLGT LYQLMESRVK TFQKLSHLHG KLILLITQVT ASEKTKGATS PGQKAKLVYE 610 620 630 640 650 660 EESSEEESDD EIADKDSEDN WDEDEEESES EKDEDVEEED EDAEGKDEEN GEDRDTASEK 670 ELNGDSDLDP ENESEEE

References

[1]	"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[2]	"Functional proteomic analysis of human nucleolus." Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C., Greco A., Hochstrasser D.F., Diaz J.-J. Mol. Biol. Cell 13:4100-4109(2002) [PubMed: 12429849] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[3]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; SER-603; SER-604 AND SER-608, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[4]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, MASS SPECTROMETRY. Tissue: Cervix adenocarcinoma.
[6]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-394 AND SER-431, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Evaluation of the low-specificity protease elastase for large-scale phosphoproteome analysis." Wang B., Malik R., Nigg E.A., Korner R. Anal. Chem. 80:9526-9533(2008) [PubMed: 19007248] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77; THR-394; SER-431 AND SER-437, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-431, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26488 mRNA. Translation: BAA05499.1. Different initiation. D87716 mRNA. Translation: BAA13441.1. Frameshift.
IPI	IPI00937477.
RefSeq	NP_055946.1. NM_015131.1.
UniGene	Hs.622982. Hs.709228.
3D structure databases
ProteinModelPortal	Q15061.
SMR	Q15061. Positions 17-305.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q15061. 2 interactions.
STRING	Q15061.
PTM databases
PhosphoSite	Q15061.
Polymorphism databases
DMDM	158518532.
2D gel databases
SWISS-2DPAGE	Q15061.
Proteomic databases
PRIDE	Q15061.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000407426; ENSP00000384302; ENSG00000163811.
GeneID	23160.
KEGG	hsa:23160.
UCSC	uc002rmo.2. human.
Organism-specific databases
CTD	23160.
GeneCards	GC02P029117.
H-InvDB	HIX0001939.
HGNC	HGNC:28945. WDR43.
HPA	HPA044733.
neXtProt	NX_Q15061.
PharmGKB	PA134914163.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG14023.
HOGENOM	HBG506445.
HOVERGEN	HBG060012.
InParanoid	Q15061.
OMA	CKVKCKW.
OrthoDB	EOG46WZ83.
PhylomeDB	Q15061.
Gene expression databases
ArrayExpress	Q15061.
Bgee	Q15061.
CleanEx	HS_WDR43.
Genevestigator	Q15061.
Family and domain databases
InterPro	IPR007148. SSU_processome_Utp12. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K14546.
Pfam	PF04003. Utp12. 1 hit. PF00400. WD40. 3 hits. [Graphical view]
SMART	SM00320. WD40. 5 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 1 hit. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	44513.

Entry information

Entry name

WDR43_HUMAN

Accession

Primary (citable) accession number: Q15061
Secondary accession number(s): Q15395, Q92577

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.