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Protein

Bromodomain-containing protein 3

Gene

BRD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.By similarity1 Publication

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • lysine-acetylated histone binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169925-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 3
Alternative name(s):
RING3-like protein
Gene namesi
Name:BRD3
Synonyms:KIAA0043, RING3L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:1104. BRD3.

Subcellular locationi

  • Nucleus Curated

  • Note: Detected on chromatin.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei647 – 648Breakpoint for translocation to form BDR3-NUT fusion protein2

Organism-specific databases

DisGeNETi8019.
OpenTargetsiENSG00000169925.
PharmGKBiPA25415.

Chemistry databases

ChEMBLiCHEMBL1795186.
GuidetoPHARMACOLOGYi2725.

Polymorphism and mutation databases

BioMutaiBRD3.
DMDMi12643726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002111812 – 726Bromodomain-containing protein 3Add BLAST725

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei263PhosphoserineCombined sources1
Modified residuei281PhosphoserineCombined sources1
Modified residuei563PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ15059.
MaxQBiQ15059.
PaxDbiQ15059.
PeptideAtlasiQ15059.
PRIDEiQ15059.

PTM databases

iPTMnetiQ15059.
PhosphoSitePlusiQ15059.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000169925.
CleanExiHS_BRD3.
ExpressionAtlasiQ15059. baseline and differential.
GenevisibleiQ15059. HS.

Organism-specific databases

HPAiHPA051830.

Interactioni

Subunit structurei

Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues (By similarity). Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYCP011063EBI-1383460,EBI-447544

GO - Molecular functioni

  • lysine-acetylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi113715. 26 interactors.
DIPiDIP-39755N.
IntActiQ15059. 9 interactors.
MINTiMINT-3030809.
STRINGi9606.ENSP00000305918.

Chemistry databases

BindingDBiQ15059.

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 31Combined sources3
Helixi37 – 44Combined sources8
Helixi46 – 52Combined sources7
Helixi57 – 59Combined sources3
Turni65 – 69Combined sources5
Helixi73 – 76Combined sources4
Helixi83 – 91Combined sources9
Helixi98 – 115Combined sources18
Helixi121 – 137Combined sources17
Helixi310 – 323Combined sources14
Helixi325 – 327Combined sources3
Helixi328 – 331Combined sources4
Helixi332 – 334Combined sources3
Turni340 – 344Combined sources5
Helixi348 – 351Combined sources4
Helixi358 – 366Combined sources9
Helixi373 – 390Combined sources18
Helixi396 – 413Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E7NNMR-A306-415[»]
2NXBX-ray1.40A/B24-144[»]
2OO1X-ray1.70A/B/C/D307-416[»]
2YW5NMR-A25-155[»]
3S91X-ray2.06A24-144[»]
3S92X-ray1.36A306-416[»]
5A7CX-ray1.90A/B/C/D306-416[»]
5HFRX-ray1.70A/B/C/D306-416[»]
5HJCX-ray2.60A307-416[»]
ProteinModelPortaliQ15059.
SMRiQ15059.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ15059.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 123Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini326 – 398Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini562 – 644NETPROSITE-ProRule annotationAdd BLAST83

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili453 – 524Sequence analysisAdd BLAST72
Coiled coili645 – 684Sequence analysisAdd BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 555Lys-richAdd BLAST69
Compositional biasi676 – 725Ser-richAdd BLAST50

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ15059.
KOiK11721.
OMAiAGRQPKK.
OrthoDBiEOG091G01QC.
PhylomeDBiQ15059.
TreeFamiTF317345.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q15059-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTATTVAPA GIPATPGPVN PPPPEVSNPS KPGRKTNQLQ YMQNVVVKTL
60 70 80 90 100
WKHQFAWPFY QPVDAIKLNL PDYHKIIKNP MDMGTIKKRL ENNYYWSASE
110 120 130 140 150
CMQDFNTMFT NCYIYNKPTD DIVLMAQALE KIFLQKVAQM PQEEVELLPP
160 170 180 190 200
APKGKGRKPA AGAQSAGTQQ VAAVSSVSPA TPFQSVPPTV SQTPVIAATP
210 220 230 240 250
VPTITANVTS VPVPPAAAPP PPATPIVPVV PPTPPVVKKK GVKRKADTTT
260 270 280 290 300
PTTSAITASR SESPPPLSDP KQAKVVARRE SGGRPIKPPK KDLEDGEVPQ
310 320 330 340 350
HAGKKGKLSE HLRYCDSILR EMLSKKHAAY AWPFYKPVDA EALELHDYHD
360 370 380 390 400
IIKHPMDLST VKRKMDGREY PDAQGFAADV RLMFSNCYKY NPPDHEVVAM
410 420 430 440 450
ARKLQDVFEM RFAKMPDEPV EAPALPAPAA PMVSKGAESS RSSEESSSDS
460 470 480 490 500
GSSDSEEERA TRLAELQEQL KAVHEQLAAL SQAPVNKPKK KKEKKEKEKK
510 520 530 540 550
KKDKEKEKEK HKVKAEEEKK AKVAPPAKQA QQKKAPAKKA NSTTTAGRQL
560 570 580 590 600
KKGGKQASAS YDSEEEEEGL PMSYDEKRQL SLDINRLPGE KLGRVVHIIQ
610 620 630 640 650
SREPSLRDSN PDEIEIDFET LKPTTLRELE RYVKSCLQKK QRKPFSASGK
660 670 680 690 700
KQAAKSKEEL AQEKKKELEK RLQDVSGQLS SSKKPARKEK PGSAPSGGPS
710 720
RLSSSSSSES GSSSSSGSSS DSSDSE
Length:726
Mass (Da):79,542
Last modified:November 1, 1996 - v1
Checksum:i64F526FC3C1033AA
GO
Isoform 2 (identifier: Q15059-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     549-556: QLKKGGKQ → DHFLTCGV
     557-726: Missing.

Note: No experimental confirmation available.
Show »
Length:556
Mass (Da):60,942
Checksum:i8352F5DF1801A793
GO

Sequence cautioni

The sequence BAA05393 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04191336T → N in a renal clear cell carcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041914161A → T in a gastric adenocarcinoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_041915172A → V.1 PublicationCorresponds to variant rs34609592dbSNPEnsembl.1
Natural variantiVAR_041916435K → Q.1 PublicationCorresponds to variant rs36093130dbSNPEnsembl.1
Natural variantiVAR_041917441R → H.1 PublicationCorresponds to variant rs56017928dbSNPEnsembl.1
Natural variantiVAR_041918447S → P.1 PublicationCorresponds to variant rs55754444dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010247549 – 556QLKKGGKQ → DHFLTCGV in isoform 2. 1 Publication8
Alternative sequenceiVSP_010248557 – 726Missing in isoform 2. 1 PublicationAdd BLAST170

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26362 mRNA. Translation: BAA05393.2. Different initiation.
AY513270 mRNA. Translation: AAS82952.1.
AL445931 Genomic DNA. Translation: CAI13726.1.
AL445931 Genomic DNA. Translation: CAI13727.1.
CH471090 Genomic DNA. Translation: EAW88113.1.
CH471090 Genomic DNA. Translation: EAW88114.1.
BC032124 mRNA. Translation: AAH32124.1.
Z81330 Genomic DNA. No translation available.
CCDSiCCDS6980.1. [Q15059-1]
RefSeqiNP_031397.1. NM_007371.3. [Q15059-1]
XP_006717354.1. XM_006717291.2. [Q15059-1]
XP_011517354.1. XM_011519052.2. [Q15059-1]
UniGeneiHs.522472.
Hs.654869.

Genome annotation databases

EnsembliENST00000303407; ENSP00000305918; ENSG00000169925. [Q15059-1]
ENST00000371834; ENSP00000360900; ENSG00000169925. [Q15059-2]
GeneIDi8019.
KEGGihsa:8019.
UCSCiuc004cew.4. human. [Q15059-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26362 mRNA. Translation: BAA05393.2. Different initiation.
AY513270 mRNA. Translation: AAS82952.1.
AL445931 Genomic DNA. Translation: CAI13726.1.
AL445931 Genomic DNA. Translation: CAI13727.1.
CH471090 Genomic DNA. Translation: EAW88113.1.
CH471090 Genomic DNA. Translation: EAW88114.1.
BC032124 mRNA. Translation: AAH32124.1.
Z81330 Genomic DNA. No translation available.
CCDSiCCDS6980.1. [Q15059-1]
RefSeqiNP_031397.1. NM_007371.3. [Q15059-1]
XP_006717354.1. XM_006717291.2. [Q15059-1]
XP_011517354.1. XM_011519052.2. [Q15059-1]
UniGeneiHs.522472.
Hs.654869.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E7NNMR-A306-415[»]
2NXBX-ray1.40A/B24-144[»]
2OO1X-ray1.70A/B/C/D307-416[»]
2YW5NMR-A25-155[»]
3S91X-ray2.06A24-144[»]
3S92X-ray1.36A306-416[»]
5A7CX-ray1.90A/B/C/D306-416[»]
5HFRX-ray1.70A/B/C/D306-416[»]
5HJCX-ray2.60A307-416[»]
ProteinModelPortaliQ15059.
SMRiQ15059.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113715. 26 interactors.
DIPiDIP-39755N.
IntActiQ15059. 9 interactors.
MINTiMINT-3030809.
STRINGi9606.ENSP00000305918.

Chemistry databases

BindingDBiQ15059.
ChEMBLiCHEMBL1795186.
GuidetoPHARMACOLOGYi2725.

PTM databases

iPTMnetiQ15059.
PhosphoSitePlusiQ15059.

Polymorphism and mutation databases

BioMutaiBRD3.
DMDMi12643726.

Proteomic databases

EPDiQ15059.
MaxQBiQ15059.
PaxDbiQ15059.
PeptideAtlasiQ15059.
PRIDEiQ15059.

Protocols and materials databases

DNASUi8019.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000303407; ENSP00000305918; ENSG00000169925. [Q15059-1]
ENST00000371834; ENSP00000360900; ENSG00000169925. [Q15059-2]
GeneIDi8019.
KEGGihsa:8019.
UCSCiuc004cew.4. human. [Q15059-1]

Organism-specific databases

CTDi8019.
DisGeNETi8019.
GeneCardsiBRD3.
HGNCiHGNC:1104. BRD3.
HPAiHPA051830.
MIMi601541. gene.
neXtProtiNX_Q15059.
OpenTargetsiENSG00000169925.
PharmGKBiPA25415.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000231200.
HOVERGENiHBG004896.
InParanoidiQ15059.
KOiK11721.
OMAiAGRQPKK.
OrthoDBiEOG091G01QC.
PhylomeDBiQ15059.
TreeFamiTF317345.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000169925-MONOMER.

Miscellaneous databases

ChiTaRSiBRD3. human.
EvolutionaryTraceiQ15059.
GeneWikiiBRD3.
GenomeRNAii8019.
PROiQ15059.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169925.
CleanExiHS_BRD3.
ExpressionAtlasiQ15059. baseline and differential.
GenevisibleiQ15059. HS.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRD3_HUMAN
AccessioniPrimary (citable) accession number: Q15059
Secondary accession number(s): B1APD9
, Q4G5Y3, Q5T1R7, Q8N5M3, Q92645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.