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Q15059 (BRD3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bromodomain-containing protein 3
Alternative name(s):
RING3-like protein
Gene names
Name:BRD3
Synonyms:KIAA0043, RING3L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets By similarity. Regulates transcription of the CCND1 gene. Ref.7

Subunit structure

Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues By similarity. Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro). Ref.7 Ref.15

Subcellular location

Nucleus Probable. Note: Detected on chromatin By similarity.

Tissue specificity

Ubiquitous.

Involvement in disease

A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.

Sequence similarities

Contains 2 bromo domains.

Contains 1 NET domain.

Sequence caution

The sequence BAA05393.2 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MYCP011063EBI-1383460,EBI-447544

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q15059-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q15059-2)

The sequence of this isoform differs from the canonical sequence as follows:
     549-556: QLKKGGKQ → DHFLTCGV
     557-726: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.10
Chain2 – 726725Bromodomain-containing protein 3
PRO_0000211181

Regions

Domain51 – 12373Bromo 1
Domain326 – 39873Bromo 2
Domain562 – 64483NET
Coiled coil453 – 52472 Potential
Coiled coil645 – 68440 Potential
Compositional bias487 – 55569Lys-rich
Compositional bias676 – 72550Ser-rich

Sites

Site647 – 6482Breakpoint for translocation to form BDR3-NUT fusion protein

Amino acid modifications

Modified residue21N-acetylserine Ref.10
Modified residue2631Phosphoserine Ref.9 Ref.12 Ref.13
Modified residue5631Phosphoserine Ref.9 Ref.13

Natural variations

Alternative sequence549 – 5568QLKKGGKQ → DHFLTCGV in isoform 2.
VSP_010247
Alternative sequence557 – 726170Missing in isoform 2.
VSP_010248
Natural variant361T → N in a renal clear cell carcinoma sample; somatic mutation. Ref.16
VAR_041913
Natural variant1611A → T in a gastric adenocarcinoma sample; somatic mutation. Ref.16
VAR_041914
Natural variant1721A → V. Ref.16
Corresponds to variant rs34609592 [ dbSNP | Ensembl ].
VAR_041915
Natural variant4351K → Q. Ref.16
Corresponds to variant rs36093130 [ dbSNP | Ensembl ].
VAR_041916
Natural variant4411R → H. Ref.16
Corresponds to variant rs56017928 [ dbSNP | Ensembl ].
VAR_041917
Natural variant4471S → P. Ref.16
Corresponds to variant rs55754444 [ dbSNP | Ensembl ].
VAR_041918

Secondary structure

................................... 726
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 64F526FC3C1033AA

FASTA72679,542
        10         20         30         40         50         60 
MSTATTVAPA GIPATPGPVN PPPPEVSNPS KPGRKTNQLQ YMQNVVVKTL WKHQFAWPFY 

        70         80         90        100        110        120 
QPVDAIKLNL PDYHKIIKNP MDMGTIKKRL ENNYYWSASE CMQDFNTMFT NCYIYNKPTD 

       130        140        150        160        170        180 
DIVLMAQALE KIFLQKVAQM PQEEVELLPP APKGKGRKPA AGAQSAGTQQ VAAVSSVSPA 

       190        200        210        220        230        240 
TPFQSVPPTV SQTPVIAATP VPTITANVTS VPVPPAAAPP PPATPIVPVV PPTPPVVKKK 

       250        260        270        280        290        300 
GVKRKADTTT PTTSAITASR SESPPPLSDP KQAKVVARRE SGGRPIKPPK KDLEDGEVPQ 

       310        320        330        340        350        360 
HAGKKGKLSE HLRYCDSILR EMLSKKHAAY AWPFYKPVDA EALELHDYHD IIKHPMDLST 

       370        380        390        400        410        420 
VKRKMDGREY PDAQGFAADV RLMFSNCYKY NPPDHEVVAM ARKLQDVFEM RFAKMPDEPV 

       430        440        450        460        470        480 
EAPALPAPAA PMVSKGAESS RSSEESSSDS GSSDSEEERA TRLAELQEQL KAVHEQLAAL 

       490        500        510        520        530        540 
SQAPVNKPKK KKEKKEKEKK KKDKEKEKEK HKVKAEEEKK AKVAPPAKQA QQKKAPAKKA 

       550        560        570        580        590        600 
NSTTTAGRQL KKGGKQASAS YDSEEEEEGL PMSYDEKRQL SLDINRLPGE KLGRVVHIIQ 

       610        620        630        640        650        660 
SREPSLRDSN PDEIEIDFET LKPTTLRELE RYVKSCLQKK QRKPFSASGK KQAAKSKEEL 

       670        680        690        700        710        720 
AQEKKKELEK RLQDVSGQLS SSKKPARKEK PGSAPSGGPS RLSSSSSSES GSSSSSGSSS 


DSSDSE 

« Hide

Isoform 2 [UniParc].

Checksum: 8352F5DF1801A793
Show »

FASTA55660,942

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow.
[2]"Differentially expressed genes in endothelial differentiation."
Ishii H., Mimori K., Mori M., Vecchione A.
DNA Cell Biol. 24:432-437(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Colon.
[6]"Chromosomal localization, gene structure and transcription pattern of the ORFX gene, a homologue of the MHC-linked RING3 gene."
Thorpe K.L., Gorman P., Thomas C., Sheer D., Trowsdale J., Beck S.
Gene 200:177-183(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 363-726.
[7]"The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription."
LeRoy G., Rickards B., Flint S.J.
Mol. Cell 30:51-60(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ACETYLATED CHROMATIN.
[8]"BRD-NUT oncoproteins: a family of closely related nuclear proteins that block epithelial differentiation and maintain the growth of carcinoma cells."
French C.A., Ramirez C.L., Kolmakova J., Hickman T.T., Cameron M.J., Thyne M.E., Kutok J.L., Toretsky J.A., Tadavarthy A.K., Kees U.R., Fletcher J.A., Aster J.C.
Oncogene 27:2237-2242(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: CHROMOSOMAL TRANSLOCATION WITH NUT.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263 AND SER-563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263 AND SER-563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Solution structure of the first and second bromodomain from human bromodomain containing protein 3."
RIKEN structural genomics initiative (RSGI)
Submitted (FEB-2009) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 25-415.
[15]"Histone recognition and large-scale structural analysis of the human bromodomain family."
Filippakopoulos P., Picaud S., Mangos M., Keates T., Lambert J.P., Barsyte-Lovejoy D., Felletar I., Volkmer R., Muller S., Pawson T., Gingras A.C., Arrowsmith C.H., Knapp S.
Cell 149:214-231(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.36 ANGSTROMS) OF 24-416, SUBUNIT, DOMAIN.
[16]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASN-36; THR-161; VAL-172; GLN-435; HIS-441 AND PRO-447.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26362 mRNA. Translation: BAA05393.2. Different initiation.
AY513270 mRNA. Translation: AAS82952.1.
AL445931 Genomic DNA. Translation: CAI13726.1.
AL445931 Genomic DNA. Translation: CAI13727.1.
CH471090 Genomic DNA. Translation: EAW88113.1.
CH471090 Genomic DNA. Translation: EAW88114.1.
BC032124 mRNA. Translation: AAH32124.1.
Z81330 Genomic DNA. No translation available.
CCDSCCDS6980.1. [Q15059-1]
RefSeqNP_031397.1. NM_007371.3. [Q15059-1]
XP_006717354.1. XM_006717291.1. [Q15059-1]
UniGeneHs.522472.
Hs.654869.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2E7NNMR-A306-415[»]
2NXBX-ray1.40A/B24-144[»]
2OO1X-ray1.70A/B/C/D307-416[»]
2YW5NMR-A25-155[»]
3S91X-ray2.06A24-144[»]
3S92X-ray1.36A306-416[»]
ProteinModelPortalQ15059.
SMRQ15059. Positions 25-155, 307-416, 571-644.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113715. 9 interactions.
IntActQ15059. 3 interactions.
MINTMINT-3030809.
STRING9606.ENSP00000305918.

Chemistry

BindingDBQ15059.
ChEMBLCHEMBL1795186.

PTM databases

PhosphoSiteQ15059.

Polymorphism databases

DMDM12643726.

Proteomic databases

MaxQBQ15059.
PaxDbQ15059.
PRIDEQ15059.

Protocols and materials databases

DNASU8019.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000303407; ENSP00000305918; ENSG00000169925. [Q15059-1]
ENST00000357885; ENSP00000350557; ENSG00000169925. [Q15059-2]
ENST00000371834; ENSP00000360900; ENSG00000169925. [Q15059-2]
GeneID8019.
KEGGhsa:8019.
UCSCuc004cew.3. human. [Q15059-1]
uc004cex.2. human. [Q15059-2]

Organism-specific databases

CTD8019.
GeneCardsGC09M136897.
HGNCHGNC:1104. BRD3.
HPAHPA051830.
MIM601541. gene.
neXtProtNX_Q15059.
PharmGKBPA25415.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5076.
HOGENOMHOG000231200.
HOVERGENHBG004896.
InParanoidQ15059.
KOK11721.
OMARHCDSIL.
OrthoDBEOG7TTQ86.
PhylomeDBQ15059.
TreeFamTF317345.

Gene expression databases

ArrayExpressQ15059.
BgeeQ15059.
CleanExHS_BRD3.
GenevestigatorQ15059.

Family and domain databases

Gene3D1.20.920.10. 2 hits.
InterProIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamPF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMSSF47370. SSF47370. 2 hits.
PROSITEPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ15059.
GeneWikiBRD3.
GenomeRNAi8019.
NextBio30578.
PROQ15059.
SOURCESearch...

Entry information

Entry nameBRD3_HUMAN
AccessionPrimary (citable) accession number: Q15059
Secondary accession number(s): B1APD9 expand/collapse secondary AC list , Q4G5Y3, Q5T1R7, Q8N5M3, Q92645
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM