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Protein

Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2

Gene

ACAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6).1 Publication

Enzyme regulationi

GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri414 – 437C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114331-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2
Alternative name(s):
Centaurin-beta-2
Short name:
Cnt-b2
Gene namesi
Name:ACAP2
Synonyms:CENTB2, KIAA0041
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:16469. ACAP2.

Subcellular locationi

GO - Cellular componenti

  • endosome membrane Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi442R → Q: Loss of GAP activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000114331.
PharmGKBiPA26407.

Polymorphism and mutation databases

BioMutaiACAP2.
DMDMi39932727.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000742101 – 778Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2Add BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei384PhosphoserineCombined sources1
Modified residuei387PhosphoserineBy similarity1
Modified residuei521PhosphoserineCombined sources1
Modified residuei581PhosphoserineCombined sources1
Modified residuei584PhosphoserineBy similarity1
Modified residuei742PhosphotyrosineCombined sources1
Modified residuei775PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ15057.
MaxQBiQ15057.
PaxDbiQ15057.
PeptideAtlasiQ15057.
PRIDEiQ15057.

PTM databases

iPTMnetiQ15057.
PhosphoSitePlusiQ15057.

Expressioni

Tissue specificityi

Widely expressed. Highest level in lung.1 Publication

Gene expression databases

BgeeiENSG00000114331.
CleanExiHS_ACAP2.
ExpressionAtlasiQ15057. baseline and differential.
GenevisibleiQ15057. HS.

Organism-specific databases

HPAiHPA034807.
HPA034808.

Interactioni

Subunit structurei

Interacts with RAB35 (GTP-bound form); the interaction is direct and probably recruits ACAP2 to membranes. Interacts with MICALL1; the interaction is indirect through RAB35 (By similarity).By similarity

Protein-protein interaction databases

BioGridi117073. 8 interactors.
IntActiQ15057. 5 interactors.
STRINGi9606.ENSP00000324287.

Structurei

3D structure databases

ProteinModelPortaliQ15057.
SMRiQ15057.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 226BARAdd BLAST226
Domaini266 – 361PHPROSITE-ProRule annotationAdd BLAST96
Domaini399 – 520Arf-GAPPROSITE-ProRule annotationAdd BLAST122
Repeati640 – 669ANK 1Add BLAST30
Repeati673 – 702ANK 2Add BLAST30
Repeati706 – 735ANK 3Add BLAST30

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 BAR domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri414 – 437C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000220815.
HOVERGENiHBG050889.
InParanoidiQ15057.
KOiK12489.
OMAiIPGNTSC.
OrthoDBiEOG091G029F.
PhylomeDBiQ15057.
TreeFamiTF318315.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q15057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTVDFEEC LKDSPRFRAA LEEVEGDVAE LELKLDKLVK LCIAMIDTGK
60 70 80 90 100
AFCVANKQFM NGIRDLAQYS SNDAVVETSL TKFSDSLQEM INFHTILFDQ
110 120 130 140 150
TQRSIKAQLQ NFVKEDLRKF KDAKKQFEKV SEEKENALVK NAQVQRNKQH
160 170 180 190 200
EVEEATNILT ATRKCFRHIA LDYVLQINVL QSKRRSEILK SMLSFMYAHL
210 220 230 240 250
AFFHQGYDLF SELGPYMKDL GAQLDRLVVD AAKEKREMEQ KHSTIQQKDF
260 270 280 290 300
SSDDSKLEYN VDAANGIVME GYLFKRASNA FKTWNRRWFS IQNNQLVYQK
310 320 330 340 350
KFKDNPTVVV EDLRLCTVKH CEDIERRFCF EVVSPTKSCM LQADSEKLRQ
360 370 380 390 400
AWIKAVQTSI ATAYREKGDE SEKLDKKSSP STGSLDSGNE SKEKLLKGES
410 420 430 440 450
ALQRVQCIPG NASCCDCGLA DPRWASINLG ITLCIECSGI HRSLGVHFSK
460 470 480 490 500
VRSLTLDTWE PELLKLMCEL GNDVINRVYE ANVEKMGIKK PQPGQRQEKE
510 520 530 540 550
AYIRAKYVER KFVDKYSISL SPPEQQKKFV SKSSEEKRLS ISKFGPGDQV
560 570 580 590 600
RASAQSSVRS NDSGIQQSSD DGRESLPSTV SANSLYEPEG ERQDSSMFLD
610 620 630 640 650
SKHLNPGLQL YRASYEKNLP KMAEALAHGA DVNWANSEEN KATPLIQAVL
660 670 680 690 700
GGSLVTCEFL LQNGANVNQR DVQGRGPLHH ATVLGHTGQV CLFLKRGANQ
710 720 730 740 750
HATDEEGKDP LSIAVEAANA DIVTLLRLAR MNEEMRESEG LYGQPGDETY
760 770
QDIFRDFSQM ASNNPEKLNR FQQDSQKF
Length:778
Mass (Da):88,029
Last modified:December 15, 2003 - v3
Checksum:i64B620957FEE6195
GO

Sequence cautioni

The sequence BAA05064 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB41450 differs from that shown. Reason: Frameshift at positions 98, 106 and 111.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → G in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti42C → G in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti229V → G in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti252S → R in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti258E → K in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti296L → V in CAB41450 (PubMed:11062263).Curated1
Sequence conflicti564G → E in AAH60767 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238248 mRNA. Translation: CAB41450.1. Frameshift.
D26069 mRNA. Translation: BAA05064.2. Different initiation.
AK290369 mRNA. Translation: BAF83058.1.
CH471052 Genomic DNA. Translation: EAW78027.1.
BC031005 mRNA. Translation: AAH31005.1.
BC060767 mRNA. Translation: AAH60767.1.
CCDSiCCDS33924.1.
RefSeqiNP_036419.3. NM_012287.5.
UniGeneiHs.593373.

Genome annotation databases

EnsembliENST00000326793; ENSP00000324287; ENSG00000114331.
GeneIDi23527.
KEGGihsa:23527.
UCSCiuc003fun.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ238248 mRNA. Translation: CAB41450.1. Frameshift.
D26069 mRNA. Translation: BAA05064.2. Different initiation.
AK290369 mRNA. Translation: BAF83058.1.
CH471052 Genomic DNA. Translation: EAW78027.1.
BC031005 mRNA. Translation: AAH31005.1.
BC060767 mRNA. Translation: AAH60767.1.
CCDSiCCDS33924.1.
RefSeqiNP_036419.3. NM_012287.5.
UniGeneiHs.593373.

3D structure databases

ProteinModelPortaliQ15057.
SMRiQ15057.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117073. 8 interactors.
IntActiQ15057. 5 interactors.
STRINGi9606.ENSP00000324287.

PTM databases

iPTMnetiQ15057.
PhosphoSitePlusiQ15057.

Polymorphism and mutation databases

BioMutaiACAP2.
DMDMi39932727.

Proteomic databases

EPDiQ15057.
MaxQBiQ15057.
PaxDbiQ15057.
PeptideAtlasiQ15057.
PRIDEiQ15057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000326793; ENSP00000324287; ENSG00000114331.
GeneIDi23527.
KEGGihsa:23527.
UCSCiuc003fun.5. human.

Organism-specific databases

CTDi23527.
GeneCardsiACAP2.
HGNCiHGNC:16469. ACAP2.
HPAiHPA034807.
HPA034808.
MIMi607766. gene.
neXtProtiNX_Q15057.
OpenTargetsiENSG00000114331.
PharmGKBiPA26407.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000220815.
HOVERGENiHBG050889.
InParanoidiQ15057.
KOiK12489.
OMAiIPGNTSC.
OrthoDBiEOG091G029F.
PhylomeDBiQ15057.
TreeFamiTF318315.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000114331-MONOMER.

Miscellaneous databases

ChiTaRSiACAP2. human.
GeneWikiiCENTB2.
GenomeRNAii23527.
PROiQ15057.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114331.
CleanExiHS_ACAP2.
ExpressionAtlasiQ15057. baseline and differential.
GenevisibleiQ15057. HS.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 3 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACAP2_HUMAN
AccessioniPrimary (citable) accession number: Q15057
Secondary accession number(s): A8K2V4, Q8N5Z8, Q9UQR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 2003
Last modified: November 2, 2016
This is version 166 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.