Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Josephin-1

Gene

JOSD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36NucleophilePROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-5689877. Josephin domain DUBs.

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:JOSD1
Synonyms:JSPH1, KIAA0063
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100221.10.
HGNCiHGNC:28953. JOSD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36C → A: Loss of deubiquitination activity, no change in subcellular location. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000100221.
PharmGKBiPA142671644.

Polymorphism and mutation databases

BioMutaiJOSD1.
DMDMi3123051.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538391 – 202Josephin-1Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1

Post-translational modificationi

Monoubiquitinated (By similarity). Ubiquitination activates deubiquitination activity in vitro.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15040.
MaxQBiQ15040.
PaxDbiQ15040.
PeptideAtlasiQ15040.
PRIDEiQ15040.

PTM databases

iPTMnetiQ15040.
PhosphoSitePlusiQ15040.

Expressioni

Gene expression databases

BgeeiENSG00000100221.
CleanExiHS_JOSD1.
ExpressionAtlasiQ15040. baseline and differential.
GenevisibleiQ15040. HS.

Organism-specific databases

HPAiHPA001168.

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115257. 26 interactors.
IntActiQ15040. 27 interactors.
STRINGi9606.ENSP00000216039.

Structurei

3D structure databases

ProteinModelPortaliQ15040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 202JosephinPROSITE-ProRule annotationAdd BLAST180

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ15040.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ15040.
TreeFamiTF313660.

Family and domain databases

InterProiView protein in InterPro
IPR006155. Josephin.
PfamiView protein in Pfam
PF02099. Josephin. 1 hit.
SMARTiView protein in SMART
SM01246. Josephin. 1 hit.
PROSITEiView protein in PROSITE
PS50957. JOSEPHIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q15040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCVPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRDTLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKHHLRGK NCELLLVVPE EVEAHQSWRT

DV
Length:202
Mass (Da):23,198
Last modified:November 1, 1996 - v1
Checksum:i837268C393C0A5A9
GO

Sequence cautioni

Q15040: The sequence BAA06682 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05003148S → R. Corresponds to variant dbSNP:rs6001200Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31884 mRNA. Translation: BAA06682.2. Different initiation.
CR456473 mRNA. Translation: CAG30359.1.
AK291827 mRNA. Translation: BAF84516.1.
AL021707 Genomic DNA. No translation available.
CH471095 Genomic DNA. Translation: EAW60259.1.
BC015026 mRNA. Translation: AAH15026.1.
CCDSiCCDS13976.1.
RefSeqiNP_055691.1. NM_014876.5.
XP_005261933.1. XM_005261876.2.
XP_005261934.1. XM_005261877.2.
XP_005261935.1. XM_005261878.2.
XP_005261936.1. XM_005261879.2.
UniGeneiHs.3094.

Genome annotation databases

EnsembliENST00000216039; ENSP00000216039; ENSG00000100221.
GeneIDi9929.
KEGGihsa:9929.
UCSCiuc003awf.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiJOS1_HUMAN
AccessioniPrimary (citable) accession number: Q15040
Secondary accession number(s): A8K712
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot