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Protein

Josephin-1

Gene

JOSD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36NucleophilePROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-5689877 Josephin domain DUBs

Protein family/group databases

MEROPSiC86.004

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:JOSD1
Synonyms:JSPH1, KIAA0063
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000100221.10
HGNCiHGNC:28953 JOSD1
MIMi615323 gene
neXtProtiNX_Q15040

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36C → A: Loss of deubiquitination activity, no change in subcellular location. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000100221
PharmGKBiPA142671644

Polymorphism and mutation databases

BioMutaiJOSD1
DMDMi3123051

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538391 – 202Josephin-1Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineCombined sources1

Post-translational modificationi

Monoubiquitinated (By similarity). Ubiquitination activates deubiquitination activity in vitro.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ15040
MaxQBiQ15040
PaxDbiQ15040
PeptideAtlasiQ15040
PRIDEiQ15040
ProteomicsDBi60388

PTM databases

iPTMnetiQ15040
PhosphoSitePlusiQ15040

Expressioni

Gene expression databases

BgeeiENSG00000100221
CleanExiHS_JOSD1
ExpressionAtlasiQ15040 baseline and differential
GenevisibleiQ15040 HS

Organism-specific databases

HPAiHPA001168

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115257, 27 interactors
IntActiQ15040, 27 interactors
MINTiQ15040
STRINGi9606.ENSP00000216039

Structurei

3D structure databases

ProteinModelPortaliQ15040
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 202JosephinPROSITE-ProRule annotationAdd BLAST180

Phylogenomic databases

eggNOGiKOG2934 Eukaryota
ENOG4111I4J LUCA
GeneTreeiENSGT00390000009228
HOGENOMiHOG000005731
HOVERGENiHBG039523
InParanoidiQ15040
KOiK15235
OMAiREVGGTY
OrthoDBiEOG091G0NAU
PhylomeDBiQ15040
TreeFamiTF313660

Family and domain databases

InterProiView protein in InterPro
IPR006155 Josephin
PfamiView protein in Pfam
PF02099 Josephin, 1 hit
SMARTiView protein in SMART
SM01246 Josephin, 1 hit
PROSITEiView protein in PROSITE
PS50957 JOSEPHIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q15040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCVPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRDTLQEIF QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALTNVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKHHLRGK NCELLLVVPE EVEAHQSWRT

DV
Length:202
Mass (Da):23,198
Last modified:November 1, 1996 - v1
Checksum:i837268C393C0A5A9
GO

Sequence cautioni

The sequence BAA06682 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05003148S → R. Corresponds to variant dbSNP:rs6001200Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31884 mRNA Translation: BAA06682.2 Different initiation.
CR456473 mRNA Translation: CAG30359.1
AK291827 mRNA Translation: BAF84516.1
AL021707 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW60259.1
BC015026 mRNA Translation: AAH15026.1
CCDSiCCDS13976.1
RefSeqiNP_055691.1, NM_014876.5
XP_005261933.1, XM_005261876.2
XP_005261934.1, XM_005261877.2
XP_005261935.1, XM_005261878.2
XP_005261936.1, XM_005261879.2
UniGeneiHs.3094
Hs.680111

Genome annotation databases

EnsembliENST00000216039; ENSP00000216039; ENSG00000100221
GeneIDi9929
KEGGihsa:9929
UCSCiuc003awf.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiJOS1_HUMAN
AccessioniPrimary (citable) accession number: Q15040
Secondary accession number(s): A8K712
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 20, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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