Reviewed,
UniProtKB/Swiss-Prot Q15029 (U5S1_HUMAN)
Last modified
February 9, 2010.
Version 115.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 116 kDa U5 small nuclear ribonucleoprotein component Alternative name(s): U5 snRNP-specific protein, 116 kDa Short name=U5-116 kDa Elongation factor Tu GTP-binding domain-containing protein 2 hSNU114 | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 972 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the U5 snRNP complex required for pre-mRNA splicing. |
| Subunit structure | Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Binds GTP. Interacts with PRPF8. Ref.4 |
| Subcellular location | |
| Post-translational modification | Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.6 Ref.7 Ref.8 Ref.10 Ref.11 |
| Sequence similarities | Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing mRNA splicing |
| Cellular component | Nucleus Spliceosome |
| Coding sequence diversity | Polymorphism |
| Ligand | GTP-binding Nucleotide-binding |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | RNA splicing Ref.3 Inferred from Experiment. Source: Reactome mRNA processing Ref.3Traceable author statement. Source: ProtInc |
| Cellular component | Cajal body Inferred from direct assay. Source: UniProtKB nuclear speckInferred from direct assay. Source: UniProtKB spliceosomal complex Ref.3Traceable author statement. Source: ProtInc |
| Molecular function | GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.3Traceable author statement. Source: ProtInc protein binding Ref.4Inferred from physical interaction. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CD2BP2 | O95400 | 1 | EBI-357897,EBI-768015 | |
| GSTK1 | Q9Y2Q3 | 1 | EBI-357897,EBI-1053767 | |
| MYC | P01106 | 2 | EBI-357897,EBI-447544 | |
| PHLDA3 | Q9Y5J5 | 1 | EBI-357897,EBI-1055859 | |
| PRPF8 | Q6P2Q9 | 1 | EBI-357897,EBI-538479 | |
| RNPS1 | Q15287 | 1 | EBI-357897,EBI-395959 | |
| SNRNP40 | Q96DI7 | 2 | EBI-357897,EBI-538492 | |
| SNRPB | P14678 | 1 | EBI-357897,EBI-372458 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 972 | 972 | 116 kDa U5 small nuclear ribonucleoprotein component | PRO_0000091563 | |||||
Regions | |||||||||
| Nucleotide binding | 136 – 143 | 8 | GTP Potential | ||||||
| Nucleotide binding | 204 – 208 | 5 | GTP Potential | ||||||
| Nucleotide binding | 258 – 261 | 4 | GTP Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1 | 1 | N-acetylmethionine Ref.10 | ||||||
| Modified residue | 19 | 1 | Phosphoserine Ref.7 Ref.10 | ||||||
| Modified residue | 30 | 1 | Phosphothreonine Ref.10 | ||||||
| Modified residue | 86 | 1 | Phosphothreonine Ref.6 Ref.8 | ||||||
| Modified residue | 944 | 1 | Phosphoserine Ref.11 | ||||||
Natural variations | |||||||||
| Natural variant | 773 | 1 | G → V: dbSNP rs1056505. | VAR_014931 | |||||
Experimental info | |||||||||
| Sequence conflict | 321 | 1 | G → V in AAH02360. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1." Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S. DNA Res. 1:27-35(1994) [PubMed: 7584026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle. |
| [3] | "An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2." Fabrizio P., Laggerbauer B., Lauber J., Lane W.S., Luehrmann R. EMBO J. 16:4092-4106(1997) [PubMed: 9233818] [Abstract] Cited for: CHARACTERIZATION. |
| [4] | "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein." Achsel T., Ahrens K., Brahms H., Teigelkamp S., Luehrmann R. Mol. Cell. Biol. 18:6756-6766(1998) [PubMed: 9774689] [Abstract] Cited for: INTERACTION WITH PRPF8. |
| [5] | "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis." Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J. RNA 8:426-439(2002) [PubMed: 11991638] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX. |
| [6] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86, MASS SPECTROMETRY. Tissue: Epithelium. |
| [7] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY. Tissue: Epithelium. |
| [8] | "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86, MASS SPECTROMETRY. |
| [9] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [10] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19 AND THR-30, MASS SPECTROMETRY. |
| [11] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-944, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D21163 mRNA. Translation: BAA04699.2. Different initiation. BC002360 mRNA. Translation: AAH02360.1. |
| IPI | IPI00003519. |
| RefSeq | NP_001136077.1. NP_004238.3. |
| UniGene | Hs.151787 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q15029. 18 interactions. |
| STRING | Q15029. |
PTM databases | |
| PhosphoSite | Q15029. |
Proteomic databases | |
| PeptideAtlas | Q15029. |
| PRIDE | Q15029. |
Genome annotation databases | |
| Ensembl | ENST00000262414; ENSP00000262414; ENSG00000108883; Homo sapiens. [Genome view] ENST00000426333; ENSP00000392094; ENSG00000108883; Homo sapiens. [Genome view] |
| GeneID | 9343. |
| KEGG | hsa:9343. |
| UCSC | uc002ihn.1. human. |
Organism-specific databases | |
| CTD | 9343. |
| GeneCards | GC17M040285. |
| H-InvDB | HIX0013887. HIX0039342. |
| HGNC | HGNC:30858. EFTUD2. |
| HPA | HPA022021. |
| MIM | 603892. gene. |
| PharmGKB | PA142671915. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG07288. |
| HOGENOM | HBG737692. |
| HOVERGEN | Q15029. |
| InParanoid | Q15029. |
| OMA | WVLIEGI. |
| PhylomeDB | Q15029. |
Enzyme and pathway databases | |
| Reactome | REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_71. Gene Expression. |
Gene expression databases | |
| ArrayExpress | Q15029. |
| Bgee | Q15029. |
| CleanEx | HS_EFTUD2. |
| Genevestigator | Q15029. |
| GermOnline | ENSG00000108883. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP_bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP_bd. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view] |
| Gene3D | G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. |
| Pfam | PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view] |
| PRINTS | PR00315. ELONGATNFCT. |
| SMART | SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00231. small_GTP. 1 hit. |
| PROSITE | PS00301. EFACTOR_GTP. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 34995. |
| SOURCE | Search... |
Entry information
| Entry name | U5S1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q15029 Secondary accession number(s): Q9BUR0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


